BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060104.seq (680 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 137 2e-33 SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 76 4e-15 SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 64 2e-11 SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 63 3e-11 SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 56 4e-09 SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 52 6e-08 SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 49 7e-07 SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc... 30 0.36 SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr ... 27 2.5 SPAC806.02c |||Par A family ATPase iron cluster assembly protein... 27 3.3 SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 26 4.4 SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces po... 26 4.4 SPCC777.11 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 26 4.4 >SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Schizosaccharomyces pombe|chr 1|||Manual Length = 272 Score = 137 bits (331), Expect = 2e-33 Identities = 69/141 (48%), Positives = 88/141 (62%) Frame = +1 Query: 91 MFRNQYDSDVTVWSPQGRLHQVGVCNGSCETWFCNHWSEE*GLCGVDSVETRCERLSAYQ 270 MFRNQYD D T WSPQGRLHQV + + + +++ E LS+YQ Sbjct: 1 MFRNQYDGDATTWSPQGRLHQVEYALEAIKQGSATVGLVSKTHAVLVALKRNAEELSSYQ 60 Query: 271 KKIIPIDEHIGISISGLTADARMLSRYMRTECLNHRYSHDAPMPVGRLISSVGNKMQICT 450 KK+I ID+HIGI+I+GL DAR+LS YM+ E L+ + P+PV RL+S V K QI T Sbjct: 61 KKLIRIDDHIGIAIAGLAPDARVLSNYMKQEALSSKTLFTRPIPVRRLMSKVAEKAQINT 120 Query: 451 QRYDKRPLGVGLLVAGYDDQG 513 Q Y +RP GVG LV GYD+ G Sbjct: 121 QEYGRRPYGVGFLVIGYDESG 141 >SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 247 Score = 76.2 bits (179), Expect = 4e-15 Identities = 49/153 (32%), Positives = 79/153 (51%), Gaps = 8/153 (5%) Frame = +1 Query: 91 MFRNQYDSDVTVWSPQGRLHQVGVCNGSCE--TWFCNHWSEE*GLCGVDSVETRCERLSA 264 M R++YD V +SP+GRL QV + + + +++ + GV+ T S Sbjct: 3 MTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKDAVVLGVEKRLTSPLMESH 62 Query: 265 YQKKIIPIDEHIGISISGLTADARMLSRYMRTECLNHRYSHDAPMPVGRLISSVGN---- 432 +K+ ID HIG +ISGLTADAR + + R + NHR+++D P + S+ + Sbjct: 63 SVEKLFEIDSHIGCAISGLTADARTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALR 122 Query: 433 --KMQICTQRYDKRPLGVGLLVAGYDDQGXSYF 525 + + +R RP GV LL+AG D+ G + Sbjct: 123 FGEGEDGEERIMSRPFGVALLIAGIDEHGPQLY 155 >SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 63.7 bits (148), Expect = 2e-11 Identities = 49/148 (33%), Positives = 72/148 (48%), Gaps = 8/148 (5%) Frame = +1 Query: 106 YDSDVTVWSPQGRLHQVGVCNGSCETWFCNHWSEE*GLCG-----VDSVETRCERL--SA 264 +D +TV+SP+GRL+QV + F N G+ G V S + ++L ++ Sbjct: 7 FDRTITVFSPEGRLYQVEYAFKA----FNNAGITSVGVTGKNCACVISQKKVPDKLIDAS 62 Query: 265 YQKKIIPIDEHIGISISGLTADARMLSRYMRTECLNHRYSHDAPMPVGRLISSVGNKMQI 444 K + PI + IG ++G ADAR R+E Y + PMP L + N Q+ Sbjct: 63 TVKHVFPITKGIGCVMTGSIADARAQVSRARSEAAEFEYKNGYPMPCDVLAKRMANIAQV 122 Query: 445 CTQRYDKRPLGVGLLVAGYDDQ-GXSYF 525 TQR RPLGV + + DD+ G S F Sbjct: 123 STQRAAMRPLGVAMTLVAVDDEIGPSLF 150 >SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces pombe|chr 2|||Manual Length = 259 Score = 63.3 bits (147), Expect = 3e-11 Identities = 45/136 (33%), Positives = 67/136 (49%), Gaps = 3/136 (2%) Frame = +1 Query: 106 YDSDVTVWSPQGRLHQVGVCNGSCE--TWFCNHWSEE*GLCGVDSVET-RCERLSAYQKK 276 YD ++V+SP GRL QV + T E + GV+ + + +S +QK Sbjct: 4 YDRALSVFSPDGRLLQVEYGQEAVRRGTTAIALRGNECIVIGVERKNVPKLQNVSNFQK- 62 Query: 277 IIPIDEHIGISISGLTADARMLSRYMRTECLNHRYSHDAPMPVGRLISSVGNKMQICTQR 456 I +D H+ ++ +GL ADAR+L R E NH+ + P+ + L V Q TQ Sbjct: 63 IAMVDNHVCLAFAGLNADARILIDKARVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQS 122 Query: 457 YDKRPLGVGLLVAGYD 504 RP GV L+AG+D Sbjct: 123 GGVRPFGVSTLIAGFD 138 >SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 248 Score = 56.4 bits (130), Expect = 4e-09 Identities = 42/141 (29%), Positives = 67/141 (47%), Gaps = 8/141 (5%) Frame = +1 Query: 106 YDSDVTVWSPQGRLHQVGVCNGSCETWFCNHWSEE*GLCGVDSVETRCERLSAYQ----- 270 YDS T++SP+GRL+QV + NH G+ D + E+ + Sbjct: 5 YDSRTTIFSPEGRLYQVEYALEAI-----NHAGVALGIVAKDGIVLAAEKKVTSKLLEQE 59 Query: 271 ---KKIIPIDEHIGISISGLTADARMLSRYMRTECLNHRYSHDAPMPVGRLISSVGNKMQ 441 +K+ I +++ +++GLTADA +L Y R + + + MP +L+ V + Q Sbjct: 60 ESAEKLYHIGDNMLCAVAGLTADANILINYARRVGQQYLQTFNEEMPCEQLVRRVCDLKQ 119 Query: 442 ICTQRYDKRPLGVGLLVAGYD 504 TQ RP GV L AG+D Sbjct: 120 GYTQYGGLRPFGVSFLYAGWD 140 >SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharomyces pombe|chr 3|||Manual Length = 253 Score = 52.4 bits (120), Expect = 6e-08 Identities = 38/131 (29%), Positives = 60/131 (45%), Gaps = 2/131 (1%) Frame = +1 Query: 127 WSPQGRLHQVGVCNGSCETWF-CNHWSEE*G-LCGVDSVETRCERLSAYQKKIIPIDEHI 300 +SP GRL Q + E C E G + ++ V T +I +D HI Sbjct: 14 FSPDGRLFQAEYAYKAVENASTCIGIKCEDGVILALEKVVTSKLLKPRVNNRIGSVDRHI 73 Query: 301 GISISGLTADARMLSRYMRTECLNHRYSHDAPMPVGRLISSVGNKMQICTQRYDKRPLGV 480 GI+ +G D + + + R E + R ++ +P+P + +GN +Q+ T RP GV Sbjct: 74 GIATTGFIPDGQHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFGV 133 Query: 481 GLLVAGYDDQG 513 VA YD +G Sbjct: 134 MSFVATYDSEG 144 >SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces pombe|chr 3|||Manual Length = 245 Score = 48.8 bits (111), Expect = 7e-07 Identities = 43/164 (26%), Positives = 73/164 (44%), Gaps = 8/164 (4%) Frame = +1 Query: 100 NQYDSDVTVWSPQGRLHQVGVCNGSCETWFCNHWSEE*GLCGVDSVETRCER-------L 258 ++Y +T +SP G+L Q+ + N G+ D V E+ + Sbjct: 3 DKYAFSLTTFSPNGKLVQIEYALNAV-----NAGVTSVGIKATDGVVLATEKKPTSELAI 57 Query: 259 SAYQKKIIPIDEHIGISISGLTADARML-SRYMRTECLNHRYSHDAPMPVGRLISSVGNK 435 A +K+ I IG+ SG+ D R+L + + ++ ++ P L+ + + Sbjct: 58 GASLEKVCAITPDIGMVYSGMGPDFRVLVDKSRKVAQTTYKKIYNEYPPTKILVQEIASV 117 Query: 436 MQICTQRYDKRPLGVGLLVAGYDDQGXSYFTRLVRQANYFDCRA 567 MQ TQ RP GV LLVAG D++G S + ++ YF +A Sbjct: 118 MQESTQSGGVRPFGVSLLVAGMDEKGPSLY-QVDPSGTYFAWKA 160 >SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 29.9 bits (64), Expect = 0.36 Identities = 21/81 (25%), Positives = 41/81 (50%), Gaps = 2/81 (2%) Frame = +1 Query: 274 KIIPIDEHIGISISGLTADARMLSRYMRTECLNHR--YSHDAPMPVGRLISSVGNKMQIC 447 K+ + ++I SG AD + ++ ++ +R + HD + ++S ++C Sbjct: 57 KLTQLTDNIWCCRSGSAADTQTVADLLKYYLSMYRIQFGHDPSVHTAATLAS-----EMC 111 Query: 448 TQRYDKRPLGVGLLVAGYDDQ 510 Q +K L GL+VAGYD++ Sbjct: 112 YQ--NKNMLSAGLIVAGYDEK 130 >SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr 1|||Manual Length = 450 Score = 27.1 bits (57), Expect = 2.5 Identities = 16/48 (33%), Positives = 21/48 (43%), Gaps = 3/48 (6%) Frame = -1 Query: 260 DNRSQRVSTLSTPHSPYSSDQWLQNQVSQLPLH---TPT*WRRP*GLH 126 D ++T P S Y S +W + QLPL T +RR G H Sbjct: 230 DEERYLIATSEQPISAYHSGEWFEKPSEQLPLKYAGYSTCYRREAGSH 277 >SPAC806.02c |||Par A family ATPase iron cluster assembly protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 608 Score = 26.6 bits (56), Expect = 3.3 Identities = 11/28 (39%), Positives = 16/28 (57%) Frame = +1 Query: 280 IPIDEHIGISISGLTADARMLSRYMRTE 363 +PID G I LT D+ ++ Y +TE Sbjct: 231 VPIDPKFGEMIENLTPDSNIVHLYSKTE 258 >SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 272 Score = 26.2 bits (55), Expect = 4.4 Identities = 19/85 (22%), Positives = 38/85 (44%) Frame = +1 Query: 271 KKIIPIDEHIGISISGLTADARMLSRYMRTECLNHRYSHDAPMPVGRLISSVGNKMQICT 450 KK+I I+ ++ +++G AD + + EC H+ + + V S+ + T Sbjct: 93 KKVIEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRNKELISV----SAASKILSNIT 148 Query: 451 QRYDKRPLGVGLLVAGYDDQGXSYF 525 Y L +G ++AG G + + Sbjct: 149 YSYKGYGLSMGTMLAGTGKGGTALY 173 >SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 594 Score = 26.2 bits (55), Expect = 4.4 Identities = 12/40 (30%), Positives = 22/40 (55%), Gaps = 1/40 (2%) Frame = +3 Query: 276 NYSY**TYWNFYIRSYCRCSYAESLHAHRVSQ-SPIFS*C 392 NYS YW+F++RS+ + +H H + + ++S C Sbjct: 14 NYSKRKNYWHFWLRSFMSNNRKFLVHIHLIPHLNSLYSIC 53 >SPCC777.11 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 128 Score = 26.2 bits (55), Expect = 4.4 Identities = 9/13 (69%), Positives = 10/13 (76%) Frame = -3 Query: 648 QXPWNVFENASSR 610 Q PWNVF+NA R Sbjct: 93 QEPWNVFQNAKKR 105 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,814,754 Number of Sequences: 5004 Number of extensions: 58380 Number of successful extensions: 168 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 153 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 157 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 313902888 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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