Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060100.seq
(670 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 326 2e-90
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 171 6e-44
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 171 1e-43
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 161 6e-41
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.4
SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22 |Schiz... 27 3.2
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 4.3
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 26 5.6
SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi... 25 7.5
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 7.5
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 25 9.9
SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 9.9
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 326 bits (801), Expect = 2e-90
Identities = 147/202 (72%), Positives = 170/202 (84%)
Frame = +1
Query: 64 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGQVR 243
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GG+
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 244 APRHLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQXGAGNNWAKGHYTEGAELVDSVLDVV 423
LVDLEPGTMD+V+SG FG +FRPDN ++GQ GAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 424 RKEAESCDCLQGFQLTHSLGGXHQFRYGPLLISKIREEYPDRIMNTYSVVPSPKVSDTVV 603
R+EAE+CD LQGFQLTHSLGG G LL+SKIREEYPDR+M T+SV P+PK SDTVV
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180
Query: 604 EPYNATLSVHQLVENTDETYCI 669
EPYNATLS+HQLVEN+DET+CI
Sbjct: 181 EPYNATLSMHQLVENSDETFCI 202
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 171 bits (417), Expect = 6e-44
Identities = 84/205 (40%), Positives = 124/205 (60%), Gaps = 3/205 (1%)
Frame = +1
Query: 64 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGQ 237
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E G+
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 238 VRAPRHL-VDLEPGTMDSVRSGPFGQIFRPDNFVFGQXGAGNNWAKGHYTEGAELVDSVL 414
PR + VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V
Sbjct: 61 Y-VPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVT 119
Query: 415 DVVRKEAESCDCLQGFQLTHSLGGXHQFRYGPLLISKIREEYPDRIMNTYSVVPSPKVSD 594
D +R+ A++C LQGF + HS GG +G LL+ ++ EY + +SV P+P+VS
Sbjct: 120 DKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVST 179
Query: 595 TVVEPYNATLSVHQLVENTDETYCI 669
+VVEPYN+ L+ H ++ D T+ +
Sbjct: 180 SVVEPYNSVLTTHATLDLADCTFMV 204
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 171 bits (415), Expect = 1e-43
Identities = 87/210 (41%), Positives = 129/210 (61%), Gaps = 8/210 (3%)
Frame = +1
Query: 64 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASG---- 231
MRE++ + GQ G QIG WE+ EHGI P G +S++ + N Y N+ G
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVH--KNNSYLNDGFGTFFS 58
Query: 232 --GQVR-APRHL-VDLEPGTMDSVRSGPFGQIFRPDNFVFGQXGAGNNWAKGHYTEGAEL 399
GQ + PR + VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E+
Sbjct: 59 ETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEM 118
Query: 400 VDSVLDVVRKEAESCDCLQGFQLTHSLGGXHQFRYGPLLISKIREEYPDRIMNTYSVVPS 579
+DSVL+ +R+ A++C LQGF + HS GG G LL+ ++ EY + +SV P+
Sbjct: 119 IDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPA 178
Query: 580 PKVSDTVVEPYNATLSVHQLVENTDETYCI 669
P+VS +VVEPYN+ L+ H ++N+D T+ +
Sbjct: 179 PQVSTSVVEPYNSVLTTHATLDNSDCTFMV 208
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 161 bits (392), Expect = 6e-41
Identities = 75/199 (37%), Positives = 123/199 (61%), Gaps = 3/199 (1%)
Frame = +1
Query: 67 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGQVRA 246
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 247 PRHLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQ--XGAGNNWAKGHYTEGAELVDSVLDV 420
L+DLEP ++++ S +G ++ P+N + + GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 421 VRKEAESCDCLQGFQLTHSLGGXHQFRYGPLLISKIREEYPDRIMNTYSVVP-SPKVSDT 597
+ +EA+ D L+GF L HS+ G G L+ ++ + YP +I+ TYSV P S VSD
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181
Query: 598 VVEPYNATLSVHQLVENTD 654
VV+PYN+ L++ +L N D
Sbjct: 182 VVQPYNSLLALKRLTLNAD 200
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.4
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 103 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 204
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 526
Score = 26.6 bits (56), Expect = 3.2
Identities = 17/70 (24%), Positives = 31/70 (44%)
Frame = +1
Query: 178 DSDLQLERINVYYNEASGGQVRAPRHLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQXGAG 357
DS L R++ + G + PR L++ M ++R + P N++FG+ G
Sbjct: 80 DSRPSLRRVSSAPDSHKGVEAPPPRPLIN-----MSNIRKAQVKILKNPGNYIFGRTEYG 134
Query: 358 NNWAKGHYTE 387
G+ T+
Sbjct: 135 KRTYSGNSTK 144
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.2 bits (55), Expect = 4.3
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +1
Query: 367 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 456
A+GH G ELV + D +RK++E+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 25.8 bits (54), Expect = 5.6
Identities = 14/35 (40%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +1
Query: 298 GPFGQIFRPDNFVFGQXGAG-NNWAKGHYTEGAEL 399
GP+G +F P F+F G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPBC646.12c |gap1|src1, sar1|GTPase activating protein
Gap1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 766
Score = 25.4 bits (53), Expect = 7.5
Identities = 15/46 (32%), Positives = 27/46 (58%)
Frame = -2
Query: 216 VVYIDALQLQVRVPMVSTGGVDAVLVGDDLPELSSDLVAALTSLDM 79
++Y+DA L +++ + G A V DLP L +D V++L S+ +
Sbjct: 511 ILYVDAKTLFIQLLRLLPSGHPATRVPLDLP-LIADSVSSLKSMSL 555
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 7.5
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +1
Query: 319 RPDNFVFGQXGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 450
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 25.0 bits (52), Expect = 9.9
Identities = 14/35 (40%), Positives = 16/35 (45%)
Frame = +2
Query: 191 SWSASMYTTMKPPAGKYVPRATSSTWSPAPWTLSA 295
S +AS PP +SST SPAPW A
Sbjct: 396 SGAASPQAGHPPPWAAASTSVSSSTSSPAPWAKPA 430
>SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 673
Score = 25.0 bits (52), Expect = 9.9
Identities = 15/44 (34%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Frame = -2
Query: 546 VRVLFSDFGDEEGPIPELVXAAERVC--QLESLEAITRFRFLSN 421
+R+L S FG E ELV ER+ L + E RF+++++
Sbjct: 463 IRILKSAFGSYENFPDELVPRVERISSGHLVNSELRQRFKYMAH 506
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,626,361
Number of Sequences: 5004
Number of extensions: 51618
Number of successful extensions: 150
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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