Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060097.seq
(684 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase, put... 62 4e-10
At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase / 3-... 59 2e-09
At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase, put... 57 9e-09
At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependen... 43 2e-04
At1g72190.1 68414.m08347 oxidoreductase family protein similar t... 39 0.003
At3g28790.1 68416.m03593 expressed protein 36 0.019
At1g79870.1 68414.m09330 oxidoreductase family protein contains ... 36 0.019
At1g15290.1 68414.m01830 tetratricopeptide repeat (TPR)-containi... 34 0.10
At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibit... 30 1.6
At5g25550.1 68418.m03040 leucine-rich repeat family protein / ex... 29 2.9
At3g24550.1 68416.m03083 protein kinase family protein contains ... 29 3.8
At3g26420.1 68416.m03295 glycine-rich RNA-binding protein simila... 28 5.0
At4g17120.1 68417.m02578 expressed protein 28 6.6
At4g15810.1 68417.m02406 chloroplast outer membrane protein, put... 28 6.6
At2g40070.1 68415.m04923 expressed protein 28 6.6
At4g25540.1 68417.m03682 DNA mismatch repair protein MSH3 (MSH3)... 27 8.8
At3g20570.1 68416.m02604 plastocyanin-like domain-containing pro... 27 8.8
At1g35510.1 68414.m04407 expressed protein contains Pfam PF03138... 27 8.8
>At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to phosphoglycerate
dehydrogenase, Arabidopsis thaliana, SP:O04130
Length = 603
Score = 61.7 bits (143), Expect = 4e-10
Identities = 41/126 (32%), Positives = 66/126 (52%), Gaps = 3/126 (2%)
Frame = +3
Query: 69 GRGTLSXVLIVDGVGAKCAELLNALRN---RHHHQGQDLQGRTSYGDTQPRRSGCAFSNS 239
G G+ +L+ + +G +LL + N ++ ++L + S D RSG
Sbjct: 57 GDGSKPTILVAEKLGDAGIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGRE 116
Query: 240 SDQRGLDAGVKLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLA 419
+ + +LKVVGRAG G+DN+D+ +A + G V+NAP AN ++A E LM +A
Sbjct: 117 VFE---SSHGRLKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMA 173
Query: 420 RHVVPA 437
R+V A
Sbjct: 174 RNVAQA 179
>At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase /
3-PGDH identical to SP|O04130
Length = 624
Score = 59.3 bits (137), Expect = 2e-09
Identities = 34/90 (37%), Positives = 52/90 (57%)
Frame = +3
Query: 168 QDLQGRTSYGDTQPRRSGCAFSNSSDQRGLDAGVKLKVVGRAGAGVDNIDVDSAGKKGVG 347
+DL+ + + D RSG + + A +LKVVGRAG G+DN+D+ +A + G
Sbjct: 114 EDLKKKVAESDALIVRSGTKVTREVFEA---AKGRLKVVGRAGVGIDNVDLQAATEHGCL 170
Query: 348 VINAPGANALSACELTCTLMLVLARHVVPA 437
V+NAP AN ++A E L+ +AR+V A
Sbjct: 171 VVNAPTANTVAAAEHGIALLASMARNVAQA 200
Score = 33.9 bits (74), Expect = 0.10
Identities = 15/32 (46%), Positives = 23/32 (71%)
Frame = +1
Query: 172 ISKEELLMEIPNHDALVVRSATQVTKEVWTQA 267
+S E+L ++ DAL+VRS T+VT+EV+ A
Sbjct: 111 LSPEDLKKKVAESDALIVRSGTKVTREVFEAA 142
>At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to SP:O04130 from
[Arabidopsis thaliana]
Length = 588
Score = 57.2 bits (132), Expect = 9e-09
Identities = 26/56 (46%), Positives = 39/56 (69%)
Frame = +3
Query: 270 KLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPA 437
+LKVVGRAG G+DN+D+ +A + G V+NAP AN ++A E L+ +AR++ A
Sbjct: 109 RLKVVGRAGVGIDNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQA 164
Score = 28.7 bits (61), Expect = 3.8
Identities = 14/29 (48%), Positives = 21/29 (72%)
Frame = +1
Query: 172 ISKEELLMEIPNHDALVVRSATQVTKEVW 258
+S EEL +I DAL+VRS T+V ++V+
Sbjct: 75 LSLEELCTKISLCDALIVRSGTKVGRDVF 103
>At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependent
hydroxypyruvate reductase identical to hydroxypyruvate
reductase (HPR) GB:D85339 [Arabidopsis thaliana] (Plant
Cell Physiol 1997 Apr;38(4):449-55)
Length = 386
Score = 42.7 bits (96), Expect = 2e-04
Identities = 20/54 (37%), Positives = 28/54 (51%)
Frame = +3
Query: 276 KVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPA 437
K G +N+DV++A K G+ V N PG + EL +L L AR +V A
Sbjct: 88 KAFSNMAVGYNNVDVEAANKYGIAVGNTPGVLTETTAELAASLSLAAARRIVEA 141
>At1g72190.1 68414.m08347 oxidoreductase family protein similar to
D-3-phosphoglycerate dehydrogenase from Arabidopsis
thaliana [SP|O04130], glyoxylate reductase from Homo
sapiens (gi:6002730); contains Pfam D-isomer specific
2-hydroxyacid dehydrogenase, NAD binding domain PF02826
Length = 373
Score = 39.1 bits (87), Expect = 0.003
Identities = 21/69 (30%), Positives = 34/69 (49%), Gaps = 3/69 (4%)
Frame = +3
Query: 225 AFSNSSDQRGLDAGVKLKVVGRAGAGVDNIDVDSAGKKGVGVINAPG---ANALSACELT 395
A + D + +K++ + G G+D +D+D+A K G+ V P NA S E+
Sbjct: 100 AMTMQMDSNVISRASNIKLIMQYGVGLDGVDIDAATKHGIKVARIPSEGTGNAASCSEMA 159
Query: 396 CTLMLVLAR 422
LML L +
Sbjct: 160 IYLMLGLLK 168
>At3g28790.1 68416.m03593 expressed protein
Length = 608
Score = 36.3 bits (80), Expect = 0.019
Identities = 17/40 (42%), Positives = 22/40 (55%)
Frame = -3
Query: 376 SALAPGALITPTPFLPAESTSMLSTPAPARPTTFSFTPAS 257
S +P TPTP P ST STP P+ PT + TP++
Sbjct: 271 SGASPSGSPTPTPSTPTPSTPTPSTPTPSTPTPSTPTPST 310
Score = 33.5 bits (73), Expect = 0.13
Identities = 28/91 (30%), Positives = 39/91 (42%), Gaps = 6/91 (6%)
Frame = -3
Query: 511 GSCPASFAAKCTEPGPTCQLSAQWK------AGTTWRARTNINVQVSSHALSALAPGALI 350
GS + +K TE G SA+ K +G T++ T + S P
Sbjct: 229 GSIETNTGSK-TEAGSKSSSSAKTKEVSGGSSGNTYKDTTGSSSGASPSGSPTPTPS--- 284
Query: 349 TPTPFLPAESTSMLSTPAPARPTTFSFTPAS 257
TPTP P ST STP P+ PT + P++
Sbjct: 285 TPTPSTPTPSTPTPSTPTPSTPTPSTPAPST 315
Score = 30.3 bits (65), Expect = 1.2
Identities = 13/22 (59%), Positives = 14/22 (63%)
Frame = -3
Query: 349 TPTPFLPAESTSMLSTPAPARP 284
TPTP P ST STPAP+ P
Sbjct: 295 TPTPSTPTPSTPTPSTPAPSTP 316
>At1g79870.1 68414.m09330 oxidoreductase family protein contains
Pfam profile: PF02826 D-isomer specific 2-hydroxyacid
dehydrogenase, NAD binding domain; similar to glyoxylate
reductase from Thermococcus litoralis [gi:13515409]
Length = 313
Score = 36.3 bits (80), Expect = 0.019
Identities = 20/78 (25%), Positives = 32/78 (41%)
Frame = +3
Query: 273 LKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPAFHCAE 452
L++V G+D ID+ +KG+ V N P +L L+L L R +
Sbjct: 68 LEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCECDRYVR 127
Query: 453 SWQVGPGSVHXAAKLAGQ 506
S + G K +G+
Sbjct: 128 SGKWKQGEFQLTTKFSGK 145
>At1g15290.1 68414.m01830 tetratricopeptide repeat (TPR)-containing
protein ESTs gb|F20110 and gb|F20109 come from this gene;
contains Pfam profile PF00515: TPR Domain
Length = 1558
Score = 33.9 bits (74), Expect = 0.10
Identities = 15/39 (38%), Positives = 22/39 (56%)
Frame = +3
Query: 141 LRNRHHHQGQDLQGRTSYGDTQPRRSGCAFSNSSDQRGL 257
L N+HH++ QD+Q + Y Q G + S SS +R L
Sbjct: 1183 LLNKHHNRNQDVQQQNIYSPLQKTSKGPSLSKSSPRRAL 1221
>At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibitor
family protein low similarity to extensin [Volvox
carteri] GI:21992
Length = 312
Score = 29.9 bits (64), Expect = 1.6
Identities = 16/47 (34%), Positives = 29/47 (61%)
Frame = -3
Query: 391 SSHALSALAPGALITPTPFLPAESTSMLSTPAPARPTTFSFTPASKP 251
SS LS+L+P ++P+P + S++ S+ +P+ P S +P+S P
Sbjct: 74 SSSPLSSLSPS--LSPSPPSSSPSSAPPSSLSPSSPPPLSLSPSSPP 118
Score = 29.1 bits (62), Expect = 2.9
Identities = 14/50 (28%), Positives = 28/50 (56%)
Frame = -3
Query: 400 VQVSSHALSALAPGALITPTPFLPAESTSMLSTPAPARPTTFSFTPASKP 251
+ SS +LS + ++P+P + S++ S+ +P+ P S +P+S P
Sbjct: 20 ITTSSSSLSPSSSSPSLSPSPPSSSPSSAPPSSLSPSSPPPLSLSPSSPP 69
>At5g25550.1 68418.m03040 leucine-rich repeat family protein /
extensin family protein similar to leucine-rich
repeat/extensin 1 (GI:13809918) [Arabidopsis thaliana];
contains Pfam PF00560: Leucine Rich Repeat domains
Length = 433
Score = 29.1 bits (62), Expect = 2.9
Identities = 19/46 (41%), Positives = 25/46 (54%), Gaps = 4/46 (8%)
Frame = -3
Query: 364 PGALITPT--PFLPAESTSM--LSTPAPARPTTFSFTPASKPLWSL 239
P + I+P+ P PA S + LSTP PARP ++P P SL
Sbjct: 383 PPSQISPSSQPLAPAPSPTSPPLSTPPPARPCPPVYSPPPPPPLSL 428
>At3g24550.1 68416.m03083 protein kinase family protein contains
Pfam domain PF00069: Protein kinase domain
Length = 652
Score = 28.7 bits (61), Expect = 3.8
Identities = 19/38 (50%), Positives = 21/38 (55%)
Frame = -3
Query: 364 PGALITPTPFLPAESTSMLSTPAPARPTTFSFTPASKP 251
PG+L TP LP S S TP+P PTT S P S P
Sbjct: 70 PGSL---TPPLPQPSPSAPITPSPPSPTTPS-NPRSPP 103
>At3g26420.1 68416.m03295 glycine-rich RNA-binding protein similar
to RNA-binding protein (RZ-1) GB:BAA12064 [Nicotiana
sylvestris]; contains Pfam profile: PF00076 RNA
recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 245
Score = 28.3 bits (60), Expect = 5.0
Identities = 22/60 (36%), Positives = 24/60 (40%), Gaps = 1/60 (1%)
Frame = +3
Query: 165 GQDLQGRTSYGD-TQPRRSGCAFSNSSDQRGLDAGVKLKVVGRAGAGVDNIDVDSAGKKG 341
G DL GRT D QP + G N D RG D G + R G D GK G
Sbjct: 70 GMDLDGRTITVDKAQPHQGGAGRDNDGD-RGRDRGYD-RDRSRPSGGRGGGDCFKCGKPG 127
>At4g17120.1 68417.m02578 expressed protein
Length = 1661
Score = 27.9 bits (59), Expect = 6.6
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +3
Query: 93 LIVDGVGAKCAELLNALRNR 152
L VDG+GA C+ L L NR
Sbjct: 1604 LTVDGIGASCSRCLEVLSNR 1623
>At4g15810.1 68417.m02406 chloroplast outer membrane protein,
putative similar to chloroplast protein import component
Toc159 [Pisum sativum] GI:8489806, chloroplast outer
envelope protein 86 [Pisum sativum] GI:599958,
GTP-binding protein [Pisum sativum] GI:576509
Length = 898
Score = 27.9 bits (59), Expect = 6.6
Identities = 13/41 (31%), Positives = 21/41 (51%)
Frame = +3
Query: 153 HHHQGQDLQGRTSYGDTQPRRSGCAFSNSSDQRGLDAGVKL 275
H+H +L GDT + +G +F NSS+ + V+L
Sbjct: 491 HNHTFMELDEYEGTGDTSEKLTGSSFQNSSELLSSNHSVEL 531
>At2g40070.1 68415.m04923 expressed protein
Length = 607
Score = 27.9 bits (59), Expect = 6.6
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = -2
Query: 542 GRPCQRPRMARVLPSELRCXVYRAGSNLPA 453
G P RPR PS R +Y +GS++PA
Sbjct: 417 GPPGGRPRRQSCSPSRGRAPMYSSGSSVPA 446
>At4g25540.1 68417.m03682 DNA mismatch repair protein MSH3 (MSH3)
identical to SP|O65607 DNA mismatch repair protein MSH3
(AtMsh3) {Arabidopsis thaliana}
Length = 1081
Score = 27.5 bits (58), Expect = 8.8
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = -3
Query: 307 STPAPARPTTFSFTPASKPLWSLELLNAQPER 212
STP P T SF+P+ + L S L A P++
Sbjct: 31 STPPPKISATVSFSPSKRKLLSDHLAAASPKK 62
>At3g20570.1 68416.m02604 plastocyanin-like domain-containing
protein
Length = 203
Score = 27.5 bits (58), Expect = 8.8
Identities = 16/49 (32%), Positives = 23/49 (46%)
Frame = -3
Query: 343 TPFLPAESTSMLSTPAPARPTTFSFTPASKPLWSLELLNAQPERRGWVS 197
+P PA + S S P+P TF TPA P S + N+ +V+
Sbjct: 141 SPPSPAPAPSGESAPSPPVSGTFEMTPAPTPTTSEDTPNSAASSLSFVA 189
>At1g35510.1 68414.m04407 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'growth regulator protein -related' based on similarity
to axi 1 protein (GB:X80301) (GI:559920) from [Nicotiana
tabacum], which, due to scienitific fraud was retracted.
Retraction in: Schell J. EMBO J 1999 May 17;18(10):2908.
PMID:10400497.
Length = 568
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/54 (27%), Positives = 22/54 (40%), Gaps = 3/54 (5%)
Frame = +3
Query: 309 NIDVDSAGKKGVGVINAPGA---NALSACELTCTLMLVLARHVVPAFHCAESWQ 461
N+ + G +I A G LS C+ L+ A V+P FH W+
Sbjct: 151 NVSAAGSNSNGYFIIEANGGLNQQRLSICDAVAVAGLLNATLVIPIFHLNSVWR 204
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,859,070
Number of Sequences: 28952
Number of extensions: 270558
Number of successful extensions: 999
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 918
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 988
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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