BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060091.seq (626 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 109 3e-25 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 109 3e-25 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 89 4e-19 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 65 9e-12 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 54 2e-08 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 53 3e-08 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 49 6e-07 SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 34 0.019 SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 34 0.019 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 34 0.019 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 34 0.019 SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 28 1.3 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 27 1.7 SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 27 1.7 SPBC25H2.15 |||programmed cell death protein homolog|Schizosacch... 27 1.7 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 26 3.9 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 26 3.9 SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|c... 26 3.9 SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosacchar... 25 6.8 SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 25 6.8 SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 9.0 SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl... 25 9.0 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 109 bits (262), Expect = 3e-25 Identities = 57/86 (66%), Positives = 67/86 (77%), Gaps = 1/86 (1%) Frame = +2 Query: 254 LKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRVTDG 430 +KSTAIS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEVTAALRVTDG Sbjct: 70 IKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDG 124 Query: 431 ALXVVDCVSGVCVQTETVLRQAXAER 508 AL VVD + GVCVQTETVLRQA ER Sbjct: 125 ALVVVDTIEGVCVQTETVLRQALGER 150 Score = 105 bits (251), Expect = 7e-24 Identities = 48/70 (68%), Positives = 58/70 (82%) Frame = +3 Query: 48 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 227 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60 Query: 228 KDEQDRCITL 257 DEQ+R +T+ Sbjct: 61 ADEQERGVTI 70 Score = 37.1 bits (82), Expect = 0.002 Identities = 15/39 (38%), Positives = 24/39 (61%) Frame = +1 Query: 508 LKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVII 624 ++P++ +NK+DR YQ F R+VE+VNV+I Sbjct: 151 IRPVVVVNKVDRALLELQISQEELYQNFARVVESVNVVI 189 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 109 bits (262), Expect = 3e-25 Identities = 57/86 (66%), Positives = 67/86 (77%), Gaps = 1/86 (1%) Frame = +2 Query: 254 LKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRVTDG 430 +KSTAIS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEVTAALRVTDG Sbjct: 70 IKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDG 124 Query: 431 ALXVVDCVSGVCVQTETVLRQAXAER 508 AL VVD + GVCVQTETVLRQA ER Sbjct: 125 ALVVVDTIEGVCVQTETVLRQALGER 150 Score = 105 bits (251), Expect = 7e-24 Identities = 48/70 (68%), Positives = 58/70 (82%) Frame = +3 Query: 48 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 227 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60 Query: 228 KDEQDRCITL 257 DEQ+R +T+ Sbjct: 61 ADEQERGVTI 70 Score = 37.1 bits (82), Expect = 0.002 Identities = 15/39 (38%), Positives = 24/39 (61%) Frame = +1 Query: 508 LKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVII 624 ++P++ +NK+DR YQ F R+VE+VNV+I Sbjct: 151 IRPVVVVNKVDRALLELQISQEELYQNFARVVESVNVVI 189 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 89.4 bits (212), Expect = 4e-19 Identities = 48/85 (56%), Positives = 63/85 (74%) Frame = +2 Query: 254 LKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGA 433 +KS+AIS+FF++ I+ D++ + EK +LINLIDSPGHVDFSSEV++A R+ DGA Sbjct: 70 MKSSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSPGHVDFSSEVSSASRLCDGA 121 Query: 434 LXVVDCVSGVCVQTETVLRQAXAER 508 +VD V GVC QT TVLRQA +R Sbjct: 122 FVLVDAVEGVCSQTITVLRQAWIDR 146 Score = 66.5 bits (155), Expect = 3e-12 Identities = 30/64 (46%), Positives = 44/64 (68%) Frame = +3 Query: 66 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDR 245 +++ + + NIRN +++AHVDHGK+TL DSL++ GII+ AG RF D R+DE R Sbjct: 7 EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITR 66 Query: 246 CITL 257 IT+ Sbjct: 67 GITM 70 Score = 27.5 bits (58), Expect = 1.7 Identities = 14/39 (35%), Positives = 19/39 (48%) Frame = +1 Query: 508 LKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVII 624 +K IL +NKMDR + R+VE VN +I Sbjct: 147 IKVILVINKMDRLITELKLSPIEAHYHLLRLVEQVNAVI 185 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 64.9 bits (151), Expect = 9e-12 Identities = 33/59 (55%), Positives = 41/59 (69%) Frame = +2 Query: 317 NPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXVVDCVSGVCVQTETVLRQ 493 N Q+ EK + IN+ID+PGH+DF+ EV ALRV DGA+ V+ VSGV QT TV RQ Sbjct: 134 NEKQKTDFEKSYNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQ 192 Score = 35.1 bits (77), Expect = 0.008 Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 2/46 (4%) Frame = +3 Query: 54 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGII 185 N + E DKKR IRN+ + AH+D GK+T T+ ++ G I Sbjct: 41 NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRI 86 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 54.0 bits (124), Expect = 2e-08 Identities = 22/51 (43%), Positives = 32/51 (62%) Frame = +2 Query: 344 KGFLINLIDSPGHVDFSSEVTAALRVTDGALXVVDCVSGVCVQTETVLRQA 496 K F ID+PGHVDF EV A + ++DG + VVD + GV + T +++ A Sbjct: 207 KTFAFQCIDTPGHVDFVDEVAAPMAISDGVVLVVDVIEGVMINTTRIIKHA 257 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 53.2 bits (122), Expect = 3e-08 Identities = 24/47 (51%), Positives = 34/47 (72%) Frame = +2 Query: 356 INLIDSPGHVDFSSEVTAALRVTDGALXVVDCVSGVCVQTETVLRQA 496 INLID+PGH DF+ EV ++ V DGA+ ++D +GV QT+ V +QA Sbjct: 95 INLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAGVEAQTKVVWKQA 141 Score = 37.5 bits (83), Expect = 0.002 Identities = 21/57 (36%), Positives = 32/57 (56%), Gaps = 3/57 (5%) Frame = +3 Query: 99 NIRNMSVIAHVDHGKSTLTDSLVSKAGI---IAGARAGETRFTDTRKDEQDRCITLN 260 +IRN+ +IAH+D GK+TLT+ ++ G G+T D E+ R IT+N Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKMLYYGGFTSHFGNVDTGDT-VMDYLPAERQRGITIN 82 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 48.8 bits (111), Expect = 6e-07 Identities = 20/44 (45%), Positives = 30/44 (68%) Frame = +2 Query: 344 KGFLINLIDSPGHVDFSSEVTAALRVTDGALXVVDCVSGVCVQT 475 + +L+NLID+PGHVDF +EV +L +G + +VD G+ QT Sbjct: 122 QSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQGIQAQT 165 Score = 45.6 bits (103), Expect = 6e-06 Identities = 24/62 (38%), Positives = 38/62 (61%) Frame = +3 Query: 72 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCI 251 +RG+ + +RN +VIAH+DHGKSTL+D ++ G+I +F D + E+ R I Sbjct: 50 VRGIPQNR--VRNWAVIAHIDHGKSTLSDCILKLTGVI-NEHNFRNQFLDKLEVERRRGI 106 Query: 252 TL 257 T+ Sbjct: 107 TV 108 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.019 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +3 Query: 84 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 179 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC9B6.04c |tuf1||mitochondrial translation elongation factor EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 33.9 bits (74), Expect = 0.019 Identities = 19/57 (33%), Positives = 31/57 (54%) Frame = +3 Query: 90 KKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITLN 260 KK ++ N+ I HVDHGK+TLT ++ + A + D +E+ R IT++ Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKARGITIS 105 Score = 31.9 bits (69), Expect = 0.078 Identities = 13/37 (35%), Positives = 17/37 (45%) Frame = +2 Query: 365 IDSPGHVDFSSEVTAALRVTDGALXVVDCVSGVCVQT 475 +D PGH D+ + DGA+ VV G QT Sbjct: 121 VDCPGHADYIKNMITGAATMDGAIIVVSATDGQMPQT 157 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.019 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +3 Query: 84 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 179 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.019 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +3 Query: 84 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 179 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual Length = 686 Score = 27.9 bits (59), Expect = 1.3 Identities = 13/34 (38%), Positives = 22/34 (64%) Frame = +3 Query: 111 MSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETR 212 ++++ HVDHGK+TL D+ K+ I + G T+ Sbjct: 174 VTLMGHVDHGKTTLLDAF-RKSTIASTEHGGITQ 206 Score = 25.4 bits (53), Expect = 6.8 Identities = 13/45 (28%), Positives = 21/45 (46%) Frame = +2 Query: 356 INLIDSPGHVDFSSEVTAALRVTDGALXVVDCVSGVCVQTETVLR 490 I +D+PGH+ F + + D + VV GV QT ++ Sbjct: 222 ITFLDTPGHMAFEAMRKRGANIADIVVLVVAGDDGVKPQTVEAIK 266 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 27.5 bits (58), Expect = 1.7 Identities = 13/27 (48%), Positives = 16/27 (59%) Frame = +2 Query: 368 DSPGHVDFSSEVTAALRVTDGALXVVD 448 D+PGH DF S + A D A+ VVD Sbjct: 261 DAPGHRDFISGMIAGASSADFAVLVVD 287 Score = 25.4 bits (53), Expect = 6.8 Identities = 27/104 (25%), Positives = 49/104 (47%), Gaps = 15/104 (14%) Frame = +3 Query: 27 KNHKPSKMVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGII------- 185 K P+ +V+ V EI + K + ++ V HVD GKST+ ++ + G I Sbjct: 155 KKQNPTDLVS--VPEIFEQSNPKPVV-HLVVTGHVDSGKSTMLGRIMFELGEINSRSMQK 211 Query: 186 ---AGARAGETRFT-----DTRKDEQDRCITLNLRPSLCSSSLK 293 A +G+ F+ DT ++E+ R +T+++ + S K Sbjct: 212 LHNEAANSGKGSFSYAWLLDTTEEERARGVTMDVASTTFESDKK 255 >SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1079 Score = 27.5 bits (58), Expect = 1.7 Identities = 13/43 (30%), Positives = 25/43 (58%) Frame = +2 Query: 362 LIDSPGHVDFSSEVTAALRVTDGALXVVDCVSGVCVQTETVLR 490 +ID+PGH F++ + + + A+ V+D + G+ QT +R Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVIDIMHGLEPQTIESIR 595 >SPBC25H2.15 |||programmed cell death protein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 396 Score = 27.5 bits (58), Expect = 1.7 Identities = 16/82 (19%), Positives = 40/82 (48%) Frame = +3 Query: 102 IRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITLNLRPSLCS 281 +++ I+H++ KS+ + V+ I + + G F+D ++ ++ PS S Sbjct: 112 VKSPKAISHLEEKKSSPKEKKVNPFAITSESSRGLNPFSDATSANNPFSLSTDVNPSKPS 171 Query: 282 SSLKRKI*YSSQTLTSVKRVRK 347 S++ K ++++ S+ +K Sbjct: 172 SNVFSKPSFAAKAQQSITDQQK 193 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 26.2 bits (55), Expect = 3.9 Identities = 19/71 (26%), Positives = 32/71 (45%) Frame = -3 Query: 489 RSTVSVCTHTPDTQSTTXRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVM 310 ++TVS + + T S++ +PS + + TS S+ S S S S Sbjct: 124 QTTVSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSS 183 Query: 309 NTKSFSSSSKN 277 ++ S SSSS + Sbjct: 184 SSSSSSSSSSS 194 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 26.2 bits (55), Expect = 3.9 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +3 Query: 108 NMSVIAHVDHGKSTLTDSLVSKAGII 185 N+ I HVD GKSTL +++ G++ Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV 265 >SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 26.2 bits (55), Expect = 3.9 Identities = 13/37 (35%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Frame = +1 Query: 133 ITASQPSRTRWFPR-PVSLLAREPERPVSLTRVRTNK 240 +T S T + P P S + REP P+S R+R+++ Sbjct: 48 LTPEPSSNTFYAPSSPASAVRREPLSPMSFVRMRSHR 84 >SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 598 Score = 25.4 bits (53), Expect = 6.8 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = -1 Query: 104 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQL 9 D P + +H D ++E H RW + +LLN++ Sbjct: 318 DNPHIHYHYFDF-HKECSHMRWDRVSLLLNEI 348 >SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccharomyces pombe|chr 3|||Manual Length = 1284 Score = 25.4 bits (53), Expect = 6.8 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -3 Query: 99 SASCPSSHGSRLP*NSPF*MVYDFCSIK 16 +ASCP SH L + PF + + C IK Sbjct: 417 TASCPLSHSKLLLEHRPFQTLAEACIIK 444 >SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 25.0 bits (52), Expect = 9.0 Identities = 9/19 (47%), Positives = 13/19 (68%) Frame = +3 Query: 108 NMSVIAHVDHGKSTLTDSL 164 N+ I HV HGKST+ ++ Sbjct: 25 NIGTIGHVAHGKSTVVKAI 43 >SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonuclease Cce1|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 25.0 bits (52), Expect = 9.0 Identities = 13/32 (40%), Positives = 19/32 (59%), Gaps = 1/32 (3%) Frame = -3 Query: 348 PFSLFSRWSGFVMNTK-SFSSSSKNIEMAVDL 256 P S +S W+ V+NTK SFS ++M +L Sbjct: 168 PKSTYSYWAS-VLNTKASFSKKKSRVQMVKEL 198 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,409,274 Number of Sequences: 5004 Number of extensions: 45451 Number of successful extensions: 167 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 147 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 165 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 277683324 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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