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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060085.seq
         (677 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g19900.1 68417.m02916 glycosyl transferase-related contains P...    33   0.17 
At2g40550.1 68415.m05003 expressed protein                             30   1.6  
At1g45191.2 68414.m05184 glycosyl hydrolase family 1 protein Sin...    29   2.8  
At5g43710.1 68418.m05344 glycoside hydrolase family 47 protein s...    28   4.9  
At2g40040.1 68415.m04920 defective chloroplasts and leaves prote...    27   8.6  

>At4g19900.1 68417.m02916 glycosyl transferase-related contains Pfam
           profiles PF01535: PPR repeat, PF04572: Alpha
           1,4-glycosyltransferase conserved region, PF04488:
           Glycosyltransferase sugar-binding region containing DXD
           motif; several hypothetical proteins - Arabidopsis
           thaliana
          Length = 1302

 Score = 33.1 bits (72), Expect = 0.17
 Identities = 13/36 (36%), Positives = 25/36 (69%)
 Frame = +3

Query: 18  LGLIEMFKDQFDNINVRNLIANNQTFDLVVVEAFAD 125
           LGLIE  ++ FD ++VR ++ ++ ++ L+V+  F D
Sbjct: 796 LGLIEYAENVFDEMSVRGVVPDSSSYKLMVIGCFRD 831


>At2g40550.1 68415.m05003 expressed protein
          Length = 589

 Score = 29.9 bits (64), Expect = 1.6
 Identities = 24/70 (34%), Positives = 33/70 (47%)
 Frame = -2

Query: 658 IQRXKRY*LTCKKTRLQTIWCQCSTQNLRKSLRFLTCSFSRR*PDLIWSTRTFYKMDSSA 479
           IQ  +R  L C     +  W +CS+Q L+   RFL  +   R   +         MDSS 
Sbjct: 118 IQVWERRLLYCVPVPGKNQWTECSSQELKN--RFLDLTGQNREKRVRVDEEMTDSMDSST 175

Query: 478 KILDAGRHGS 449
             L+AGR+GS
Sbjct: 176 --LEAGRNGS 183


>At1g45191.2 68414.m05184 glycosyl hydrolase family 1 protein Since
           this genomic sequence region is unfinished, the
           annotated gene may be missing a stop codon or start
           codon
          Length = 487

 Score = 29.1 bits (62), Expect = 2.8
 Identities = 23/75 (30%), Positives = 33/75 (44%), Gaps = 1/75 (1%)
 Frame = -3

Query: 519 FGQRALFTRWIPPPRYWTLGDTGRLLSNMG-CRFSKSNCTLLRSSSIVGVSGPNCCLSNA 343
           FG    F   I     +T+G      S  G C F   NCTL  SS+   + G N  L++A
Sbjct: 179 FGNHVKFWTTINEANIFTIGGYNDGNSPPGRCSFPGRNCTLGNSSTETYIVGHNLLLAHA 238

Query: 342 LDMLAKILNSLYKRI 298
              ++++    YK I
Sbjct: 239 --SVSRLYKQKYKDI 251


>At5g43710.1 68418.m05344 glycoside hydrolase family 47 protein
           similar to mannosyl-oligosaccharide
           1,2-alpha-mannosidase IB [Mus musculus][SP|P39098]
          Length = 624

 Score = 28.3 bits (60), Expect = 4.9
 Identities = 14/33 (42%), Positives = 18/33 (54%)
 Frame = -2

Query: 529 PDLIWSTRTFYKMDSSAKILDAGRHGSVVVKYG 431
           P+LI ST   YK     + LDAGR     ++YG
Sbjct: 375 PELIESTYWLYKATRDPRYLDAGRDFVASLQYG 407


>At2g40040.1 68415.m04920 defective chloroplasts and leaves
           protein-related / DCL protein-related similar to DCL
           protein, chloroplast precursor (Defective chloroplasts
           and leaves protein) (Swiss-Prot:Q42463) [Lycopersicon
           esculentum]
          Length = 839

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 15/40 (37%), Positives = 19/40 (47%)
 Frame = -3

Query: 207 QSFPPNRSQARFGLRAQDRTGGQTPTHNRQTLPRQLNQTS 88
           QS PPN+S    G   Q +T  Q+P+  R   P Q    S
Sbjct: 733 QSQPPNQSIGNGGDDFQTQTQSQSPSQTRAQSPSQAQAQS 772


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,730,342
Number of Sequences: 28952
Number of extensions: 333084
Number of successful extensions: 843
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 822
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 843
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1428369392
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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