BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060081.seq
(575 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces po... 84 1e-17
SPAC20H4.08 |||phosphatase activator |Schizosaccharomyces pombe|... 28 1.1
SPAC23G3.11 |rpn6||19S proteasome regulatory subunit Rpn6|Schizo... 26 3.4
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 26 3.4
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 26 4.5
SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 26 4.5
SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase Alg6|Schizosac... 26 4.5
SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces... 26 4.5
>SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 942
Score = 84.2 bits (199), Expect = 1e-17
Identities = 39/76 (51%), Positives = 53/76 (69%)
Frame = +1
Query: 40 LTGANLFRQEWSSLLDELLENNKITKDQREKYKFLHIAGMVGSIDNDFCGTDMTIGTDSA 219
LTGA++FR +W L+ EL + IT +Q + Y+ L I G+VGSIDND TD+TIG S+
Sbjct: 301 LTGADIFRSDWPGLVKELEDTKAITPEQAKLYRHLTIVGLVGSIDNDMSSTDVTIGAFSS 360
Query: 220 LHRIIEAIDAIVALLI 267
LHRI EA+D+I + I
Sbjct: 361 LHRICEAVDSISSTAI 376
Score = 61.7 bits (143), Expect = 7e-11
Identities = 34/75 (45%), Positives = 43/75 (57%), Gaps = 1/75 (1%)
Frame = +3
Query: 255 STAYSHQRTFIMEVMGRNCGYXALVAALASEAYQVFIPEDPVP-NNWVXKXXXXXXXXXX 431
STA SH R FI+EVMGR+CG+ A++AALA+ A VFIPE P W +
Sbjct: 373 STAISHSRAFIVEVMGRHCGWLAVLAALATGADFVFIPERPAEVGKWQDELCNSLSSVRK 432
Query: 432 XXXXXNXIIXAEGAI 476
+ +I AEGAI
Sbjct: 433 LGKRKSIVIVAEGAI 447
Score = 36.3 bits (80), Expect = 0.003
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = +3
Query: 255 STAYSHQRTFIMEVMGRNCGYXALVAALASEAYQVFIPEDPVPNNWVXK 401
S + S +R F+ EV G GY A V L + A ++ PED + + + K
Sbjct: 743 SASASRRRVFVCEVQGGRSGYIATVGGLITGASAIYTPEDGISLDMLRK 791
>SPAC20H4.08 |||phosphatase activator |Schizosaccharomyces pombe|chr
1|||Manual
Length = 270
Score = 27.9 bits (59), Expect = 1.1
Identities = 9/30 (30%), Positives = 19/30 (63%)
Frame = +2
Query: 92 CWRTTKLLKIKGKNINFCT*LEWLVQLTMI 181
CW +++K+ G IN +WL+++T++
Sbjct: 233 CWWINEVIKVNGSLINVWRRKQWLLEVTIM 262
>SPAC23G3.11 |rpn6||19S proteasome regulatory subunit
Rpn6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 421
Score = 26.2 bits (55), Expect = 3.4
Identities = 15/44 (34%), Positives = 22/44 (50%), Gaps = 1/44 (2%)
Frame = +1
Query: 37 ELTGANLFRQEWSSLLDELLENNKI-TKDQREKYKFLHIAGMVG 165
EL + R SSL D LLE N + + + + HIA ++G
Sbjct: 310 ELASDPIIRSHLSSLYDNLLEQNLLRVVEPFSRVEVSHIAELIG 353
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 26.2 bits (55), Expect = 3.4
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +1
Query: 49 ANLFRQEWSSLLDEL 93
AN+FR EWS+ LD +
Sbjct: 491 ANMFRNEWSNFLDSV 505
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 25.8 bits (54), Expect = 4.5
Identities = 15/56 (26%), Positives = 27/56 (48%)
Frame = +1
Query: 85 DELLENNKITKDQREKYKFLHIAGMVGSIDNDFCGTDMTIGTDSALHRIIEAIDAI 252
+E E ++ + +R K + S DND D T+G +AL I ++D++
Sbjct: 159 EESAERRRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTTVGIGAALAASILSVDSV 214
>SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 689
Score = 25.8 bits (54), Expect = 4.5
Identities = 8/24 (33%), Positives = 15/24 (62%)
Frame = +1
Query: 70 WSSLLDELLENNKITKDQREKYKF 141
W +++D L E + + ++E YKF
Sbjct: 326 WDAMIDSLYETINVEETKKETYKF 349
>SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase
Alg6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 506
Score = 25.8 bits (54), Expect = 4.5
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -3
Query: 153 SYVQKFIFFPLIFSNFVVLQQFI 85
SY KF+FFP + + V +Q I
Sbjct: 20 SYSSKFLFFPCLIMSLVFMQWLI 42
>SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 446
Score = 25.8 bits (54), Expect = 4.5
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +1
Query: 40 LTGANLFRQEWSSLLDELLENNKITKDQREKY 135
L G+ + +E S +D+L+E KI +RE++
Sbjct: 41 LKGSKTWLEELESTIDDLIEVKKIGAKEREEH 72
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,280,040
Number of Sequences: 5004
Number of extensions: 41993
Number of successful extensions: 111
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 111
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 246098644
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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