BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060081.seq (575 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces po... 84 1e-17 SPAC20H4.08 |||phosphatase activator |Schizosaccharomyces pombe|... 28 1.1 SPAC23G3.11 |rpn6||19S proteasome regulatory subunit Rpn6|Schizo... 26 3.4 SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 26 3.4 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 26 4.5 SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 26 4.5 SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase Alg6|Schizosac... 26 4.5 SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces... 26 4.5 >SPBC16H5.02 |pfk1||6-phosphofructokinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 942 Score = 84.2 bits (199), Expect = 1e-17 Identities = 39/76 (51%), Positives = 53/76 (69%) Frame = +1 Query: 40 LTGANLFRQEWSSLLDELLENNKITKDQREKYKFLHIAGMVGSIDNDFCGTDMTIGTDSA 219 LTGA++FR +W L+ EL + IT +Q + Y+ L I G+VGSIDND TD+TIG S+ Sbjct: 301 LTGADIFRSDWPGLVKELEDTKAITPEQAKLYRHLTIVGLVGSIDNDMSSTDVTIGAFSS 360 Query: 220 LHRIIEAIDAIVALLI 267 LHRI EA+D+I + I Sbjct: 361 LHRICEAVDSISSTAI 376 Score = 61.7 bits (143), Expect = 7e-11 Identities = 34/75 (45%), Positives = 43/75 (57%), Gaps = 1/75 (1%) Frame = +3 Query: 255 STAYSHQRTFIMEVMGRNCGYXALVAALASEAYQVFIPEDPVP-NNWVXKXXXXXXXXXX 431 STA SH R FI+EVMGR+CG+ A++AALA+ A VFIPE P W + Sbjct: 373 STAISHSRAFIVEVMGRHCGWLAVLAALATGADFVFIPERPAEVGKWQDELCNSLSSVRK 432 Query: 432 XXXXXNXIIXAEGAI 476 + +I AEGAI Sbjct: 433 LGKRKSIVIVAEGAI 447 Score = 36.3 bits (80), Expect = 0.003 Identities = 17/49 (34%), Positives = 26/49 (53%) Frame = +3 Query: 255 STAYSHQRTFIMEVMGRNCGYXALVAALASEAYQVFIPEDPVPNNWVXK 401 S + S +R F+ EV G GY A V L + A ++ PED + + + K Sbjct: 743 SASASRRRVFVCEVQGGRSGYIATVGGLITGASAIYTPEDGISLDMLRK 791 >SPAC20H4.08 |||phosphatase activator |Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 27.9 bits (59), Expect = 1.1 Identities = 9/30 (30%), Positives = 19/30 (63%) Frame = +2 Query: 92 CWRTTKLLKIKGKNINFCT*LEWLVQLTMI 181 CW +++K+ G IN +WL+++T++ Sbjct: 233 CWWINEVIKVNGSLINVWRRKQWLLEVTIM 262 >SPAC23G3.11 |rpn6||19S proteasome regulatory subunit Rpn6|Schizosaccharomyces pombe|chr 1|||Manual Length = 421 Score = 26.2 bits (55), Expect = 3.4 Identities = 15/44 (34%), Positives = 22/44 (50%), Gaps = 1/44 (2%) Frame = +1 Query: 37 ELTGANLFRQEWSSLLDELLENNKI-TKDQREKYKFLHIAGMVG 165 EL + R SSL D LLE N + + + + HIA ++G Sbjct: 310 ELASDPIIRSHLSSLYDNLLEQNLLRVVEPFSRVEVSHIAELIG 353 >SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual Length = 1489 Score = 26.2 bits (55), Expect = 3.4 Identities = 9/15 (60%), Positives = 12/15 (80%) Frame = +1 Query: 49 ANLFRQEWSSLLDEL 93 AN+FR EWS+ LD + Sbjct: 491 ANMFRNEWSNFLDSV 505 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 25.8 bits (54), Expect = 4.5 Identities = 15/56 (26%), Positives = 27/56 (48%) Frame = +1 Query: 85 DELLENNKITKDQREKYKFLHIAGMVGSIDNDFCGTDMTIGTDSALHRIIEAIDAI 252 +E E ++ + +R K + S DND D T+G +AL I ++D++ Sbjct: 159 EESAERRRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTTVGIGAALAASILSVDSV 214 >SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 689 Score = 25.8 bits (54), Expect = 4.5 Identities = 8/24 (33%), Positives = 15/24 (62%) Frame = +1 Query: 70 WSSLLDELLENNKITKDQREKYKF 141 W +++D L E + + ++E YKF Sbjct: 326 WDAMIDSLYETINVEETKKETYKF 349 >SPBC342.01c |alg6|SPBC3F6.06c|glucosyltransferase Alg6|Schizosaccharomyces pombe|chr 2|||Manual Length = 506 Score = 25.8 bits (54), Expect = 4.5 Identities = 10/23 (43%), Positives = 14/23 (60%) Frame = -3 Query: 153 SYVQKFIFFPLIFSNFVVLQQFI 85 SY KF+FFP + + V +Q I Sbjct: 20 SYSSKFLFFPCLIMSLVFMQWLI 42 >SPBC20F10.03 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 25.8 bits (54), Expect = 4.5 Identities = 11/32 (34%), Positives = 20/32 (62%) Frame = +1 Query: 40 LTGANLFRQEWSSLLDELLENNKITKDQREKY 135 L G+ + +E S +D+L+E KI +RE++ Sbjct: 41 LKGSKTWLEELESTIDDLIEVKKIGAKEREEH 72 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,280,040 Number of Sequences: 5004 Number of extensions: 41993 Number of successful extensions: 111 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 107 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 111 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 246098644 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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