BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060071.seq (659 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_38524| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 1.1 SB_50340| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.7 SB_14338| Best HMM Match : Spore_permease (HMM E-Value=0.75) 28 7.7 >SB_38524| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 433 Score = 30.7 bits (66), Expect = 1.1 Identities = 14/45 (31%), Positives = 24/45 (53%) Frame = +1 Query: 328 LKPLKRSDLYTRTDNFYFWCWLYYSIRF*MPFLFFX*LDEFTISK 462 +KPLKR L TR + W+Y + F +P +F +++ +K Sbjct: 135 VKPLKRKSLLTRPFSLLLSAWVYAGVLF-LPTFYFTKVEQLLTNK 178 >SB_50340| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 162 Score = 27.9 bits (59), Expect = 7.7 Identities = 25/77 (32%), Positives = 38/77 (49%), Gaps = 1/77 (1%) Frame = +1 Query: 298 DKQYLKAVKALKPLKRSDLYTRTDNFYFWCWLYYSI-RF*MPFLFFX*LDEFTISKLIRF 474 D+Q +K + A++ LKRS ++ R D +C L SI + P + L EF +S I Sbjct: 11 DEQKMKRIYAIRILKRSGVHVR-DIVEVYCTLIRSILEYGAP--VYSNLTEF-LSNAIER 66 Query: 475 HYSRGFRSFTLKVPYND 525 R + VPY+D Sbjct: 67 VQQRALKIIFPGVPYSD 83 >SB_14338| Best HMM Match : Spore_permease (HMM E-Value=0.75) Length = 367 Score = 27.9 bits (59), Expect = 7.7 Identities = 11/37 (29%), Positives = 22/37 (59%) Frame = +1 Query: 103 RKMALVFLSHPGCVVNFYEISFN*CRVKSESNILMNL 213 R++ L FL PG + Y I ++ CR+ + S ++++ Sbjct: 5 RRIVLSFLGEPGSITCLYLIHYHRCRLTNVSVCILSI 41 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,339,897 Number of Sequences: 59808 Number of extensions: 257207 Number of successful extensions: 326 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 313 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 326 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1693527500 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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