BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060066.seq (639 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2F7.04 |pmc2|med1|RNA polymerase II holoenzyme mediator comp... 25 9.2 >SPAC2F7.04 |pmc2|med1|RNA polymerase II holoenzyme mediator complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 454 Score = 25.0 bits (52), Expect = 9.2 Identities = 8/16 (50%), Positives = 10/16 (62%) Frame = +3 Query: 549 VTWPYLNIGHLTGLPS 596 + WP N H+ GLPS Sbjct: 284 IPWPVQNYSHMLGLPS 299 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,410,656 Number of Sequences: 5004 Number of extensions: 43612 Number of successful extensions: 108 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 103 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 108 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 285732116 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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