Bombyx BLAST Result against bee

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060056.seq
         (728 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ288391-1|ABC41341.1|  630|Apis mellifera vasa protein protein.       68   1e-13
AY350618-1|AAQ57660.1|  425|Apis mellifera complementary sex det...    22   5.2  
AF388659-4|AAK71996.1| 1308|Apis mellifera NFRKB-like protein pr...    22   6.8  
EF625897-1|ABR45904.1|  684|Apis mellifera hexamerin protein.          21   9.0  

>DQ288391-1|ABC41341.1|  630|Apis mellifera vasa protein protein.
          Length = 630

 Score = 67.7 bits (158), Expect = 1e-13
 Identities = 35/64 (54%), Positives = 48/64 (75%), Gaps = 4/64 (6%)
 Frame = +3

Query: 63  YLVLDEADRMLDMGFEPQIRKII--EQIRP--DRQTLMWSATWPKEVKKLAEDYLGDYIQ 230
           +LVLDEADRMLDMGF P I K++  E + P  +RQTLM+SAT+P EV+ LA  +L +Y+ 
Sbjct: 350 FLVLDEADRMLDMGFLPSIEKMVDHETMVPLGERQTLMFSATFPDEVQHLARRFLNNYLF 409

Query: 231 INIG 242
           + +G
Sbjct: 410 LAVG 413



 Score = 59.3 bits (137), Expect = 4e-11
 Identities = 40/146 (27%), Positives = 69/146 (47%), Gaps = 3/146 (2%)
 Frame = +2

Query: 299 HEKENKLNVLLQEIGQSQEPGAKTIIFVETKRKAENISRNIRRYGWPAVCMHGDKTQQER 478
           ++K++ L  +L+    S   G  T++FVE K+KA+ I+  +    +P   +HGD+ Q++R
Sbjct: 432 NKKKDLLKEILERENDSGTLGG-TLVFVEMKKKADFIAVFLSENNYPTTSIHGDRLQRQR 490

Query: 479 DEVLYQFKEGXXXXXXXXXXXXXXXXXXVSNMXXXXXXXXXXXXTSIV--LGRTGRSKSK 652
           +E L  FK G                  + N+               V  +GRTGR  ++
Sbjct: 491 EEALADFKSG--RMSILVATAVAARGLDIKNVSHVINYDLPKGIDEYVHRIGRTGRVGNR 548

Query: 653 GTSYAFFTPSNSRQAK-DLVSVLQEA 727
           G + +FF P      + DLV +L++A
Sbjct: 549 GRATSFFDPEEDAPLRGDLVRILKQA 574



 Score = 55.2 bits (127), Expect = 6e-10
 Identities = 23/38 (60%), Positives = 30/38 (78%)
 Frame = +1

Query: 514 SILVATDVAARGLDVDGIKYVINFDYPNSSEDYIHRIG 627
           SILVAT VAARGLD+  + +VIN+D P   ++Y+HRIG
Sbjct: 503 SILVATAVAARGLDIKNVSHVINYDLPKGIDEYVHRIG 540


>AY350618-1|AAQ57660.1|  425|Apis mellifera complementary sex
           determiner protein.
          Length = 425

 Score = 22.2 bits (45), Expect = 5.2
 Identities = 10/34 (29%), Positives = 19/34 (55%)
 Frame = +2

Query: 383 ETKRKAENISRNIRRYGWPAVCMHGDKTQQERDE 484
           E ++K+    R  R+YG  +     D+T++ER +
Sbjct: 277 EREQKSYKNEREYRKYGETSKERSRDRTERERSK 310


>AF388659-4|AAK71996.1| 1308|Apis mellifera NFRKB-like protein
            protein.
          Length = 1308

 Score = 21.8 bits (44), Expect = 6.8
 Identities = 10/29 (34%), Positives = 17/29 (58%)
 Frame = +2

Query: 233  QYRIITTSANHNILQIVDICQEHEKENKL 319
            Q  I+T  A  +ILQ  +I Q+  +++ L
Sbjct: 987  QQTIVTNQAGKSILQTANIKQQSPQQHVL 1015


>EF625897-1|ABR45904.1|  684|Apis mellifera hexamerin protein.
          Length = 684

 Score = 21.4 bits (43), Expect = 9.0
 Identities = 7/16 (43%), Positives = 12/16 (75%)
 Frame = +2

Query: 296 EHEKENKLNVLLQEIG 343
           ++  ENKLN  +++IG
Sbjct: 213 DYNLENKLNYFIEDIG 228


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 210,261
Number of Sequences: 438
Number of extensions: 4570
Number of successful extensions: 13
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 12
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 22657590
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)