BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060053.seq (634 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 115 5e-27 SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 115 5e-27 SPAC3H8.07c |||prefoldin subunit 3|Schizosaccharomyces pombe|chr... 27 2.3 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 2.3 SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 26 3.9 SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra... 26 5.2 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 115 bits (277), Expect = 5e-27 Identities = 48/54 (88%), Positives = 50/54 (92%) Frame = +1 Query: 256 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 417 GHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH Sbjct: 59 GHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWH 112 Score = 69.7 bits (163), Expect = 3e-13 Identities = 32/58 (55%), Positives = 42/58 (72%) Frame = +2 Query: 80 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKE 253 ++ ARP VS+Y+ K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++ Sbjct: 1 MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57 Score = 40.7 bits (91), Expect = 2e-04 Identities = 19/29 (65%), Positives = 22/29 (75%) Frame = +2 Query: 509 IEKIPELPLVVADKVQEINKTKQAVIFLK 595 IE+IPE+PLVV D VQ KTK+AV LK Sbjct: 144 IEEIPEVPLVVDDAVQSFQKTKEAVALLK 172 Score = 34.7 bits (76), Expect = 0.011 Identities = 17/60 (28%), Positives = 30/60 (50%) Frame = +3 Query: 450 LGGSVAATGVPALVQARGHILKRFPSFPWL*PTKSKXXXXXXXXXXXXXXLKAWSDILXV 629 + +VAA+GVP+L+ ARGH ++ P P + + +KA+ D++ V Sbjct: 124 IASAVAASGVPSLLLARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIKV 183 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 115 bits (277), Expect = 5e-27 Identities = 48/54 (88%), Positives = 50/54 (92%) Frame = +1 Query: 256 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 417 GHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH Sbjct: 59 GHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWH 112 Score = 70.5 bits (165), Expect = 2e-13 Identities = 33/58 (56%), Positives = 42/58 (72%) Frame = +2 Query: 80 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKE 253 ++ ARP VS+YS K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++ Sbjct: 1 MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57 Score = 40.7 bits (91), Expect = 2e-04 Identities = 19/29 (65%), Positives = 22/29 (75%) Frame = +2 Query: 509 IEKIPELPLVVADKVQEINKTKQAVIFLK 595 IE+IPE+PLVV D VQ KTK+AV LK Sbjct: 144 IEEIPEVPLVVDDAVQSFQKTKEAVALLK 172 Score = 34.3 bits (75), Expect = 0.015 Identities = 17/60 (28%), Positives = 30/60 (50%) Frame = +3 Query: 450 LGGSVAATGVPALVQARGHILKRFPSFPWL*PTKSKXXXXXXXXXXXXXXLKAWSDILXV 629 + +VAA+GVP+L+ ARGH ++ P P + + +KA+ D++ V Sbjct: 124 ISSAVAASGVPSLLLARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVKV 183 >SPAC3H8.07c |||prefoldin subunit 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 169 Score = 27.1 bits (57), Expect = 2.3 Identities = 12/21 (57%), Positives = 15/21 (71%) Frame = +2 Query: 545 DKVQEINKTKQAVIFLKAPQG 607 DK+ +I KT Q+V FLK QG Sbjct: 55 DKIPDIRKTLQSVQFLKERQG 75 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.1 bits (57), Expect = 2.3 Identities = 12/42 (28%), Positives = 17/42 (40%) Frame = +3 Query: 381 YVRPHEALAALAPSRQPPTAESGLGGSVAATGVPALVQARGH 506 + PH L + AP QP E +V P+ +GH Sbjct: 854 FYEPHSYLESPAPEPQPSYEEESFNATVIHAPTPSTATFQGH 895 >SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 26.2 bits (55), Expect = 3.9 Identities = 10/26 (38%), Positives = 17/26 (65%) Frame = -2 Query: 141 APCTVSLFSEYTDTKGRATDRLISLL 64 A C V ++SEYT G ++ L++L+ Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515 >SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltransferase Pis1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 251 Score = 25.8 bits (54), Expect = 5.2 Identities = 14/28 (50%), Positives = 20/28 (71%), Gaps = 2/28 (7%) Frame = -3 Query: 98 KVG-LPIDSFRYFSEAIPLKY-TLIAVI 21 K+G LP+ SF Y + +PL Y TL+AV+ Sbjct: 186 KLGYLPVPSFIYSTGELPLSYPTLLAVL 213 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,430,382 Number of Sequences: 5004 Number of extensions: 46882 Number of successful extensions: 135 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 123 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 133 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 281707720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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