Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021959X
(492 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1) ... 87 5e-18
At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2) ... 87 5e-18
At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2) ... 62 2e-10
At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1) ... 62 2e-10
At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1) (... 58 4e-09
At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2) ... 55 3e-08
At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1) 55 3e-08
At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1) ... 49 1e-06
At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2) ... 49 1e-06
At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1) (... 43 1e-04
At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putativ... 40 7e-04
At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1) (... 40 7e-04
At3g09930.1 68416.m01188 GDSL-motif lipase/hydrolase family prot... 32 0.24
At5g03610.1 68418.m00320 GDSL-motif lipase/hydrolase family prot... 31 0.56
At4g24460.1 68417.m03506 expressed protein 28 3.9
At4g31390.1 68417.m04452 ABC1 family protein contains Pfam domai... 27 6.9
At4g00335.2 68417.m00044 zinc finger (C3HC4-type RING finger) fa... 27 6.9
At4g00335.1 68417.m00043 zinc finger (C3HC4-type RING finger) fa... 27 6.9
>At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1)
(gb|AAC32062.1)
Length = 278
Score = 87.4 bits (207), Expect = 5e-18
Identities = 40/64 (62%), Positives = 49/64 (76%)
Frame = +1
Query: 88 MFRNQYDSDVTVWSPQGRLHQVEYAMEAVKLGSATIGLKNKDYAVLIALKRAVSEFPHIR 267
MFRNQYD+DVT WSP GRL QVEYAMEAVK GSA IGL+++ + VL + +A SE +
Sbjct: 1 MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQ 60
Query: 268 RKLF 279
RK+F
Sbjct: 61 RKIF 64
Score = 70.9 bits (166), Expect = 4e-13
Identities = 29/50 (58%), Positives = 41/50 (82%)
Frame = +2
Query: 338 SRYMRTECLNHRYSHDAPMPVGRLISSVGNKMQICTQRYDKRPLGVGLLV 487
SRYMR+E +NH +++++P+PVGRL+ + +K Q+CTQR KRP GVGLLV
Sbjct: 85 SRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCTQRSWKRPYGVGLLV 134
Score = 36.3 bits (80), Expect = 0.011
Identities = 13/28 (46%), Positives = 22/28 (78%)
Frame = +3
Query: 252 ISAYQKKIIPIDEHIGIXTPGLTAEARM 335
+S++Q+KI +D+HIG+ GLTA+ R+
Sbjct: 56 LSSHQRKIFKVDDHIGVAIAGLTADGRV 83
>At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2)
(PRC2B) (PRS1) identical to GB:AAC32063 from
[Arabidopsis thaliana] (Genetics 149 (2), 677-692
(1998)); identical to cDNA proteasome subunit prc2b
GI:2511585
Length = 277
Score = 87.4 bits (207), Expect = 5e-18
Identities = 40/64 (62%), Positives = 49/64 (76%)
Frame = +1
Query: 88 MFRNQYDSDVTVWSPQGRLHQVEYAMEAVKLGSATIGLKNKDYAVLIALKRAVSEFPHIR 267
MFRNQYD+DVT WSP GRL QVEYAMEAVK GSA IGL+++ + VL + +A SE +
Sbjct: 1 MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQ 60
Query: 268 RKLF 279
RK+F
Sbjct: 61 RKIF 64
Score = 70.9 bits (166), Expect = 4e-13
Identities = 29/50 (58%), Positives = 41/50 (82%)
Frame = +2
Query: 338 SRYMRTECLNHRYSHDAPMPVGRLISSVGNKMQICTQRYDKRPLGVGLLV 487
SRYMR+E +NH +++++P+PVGRL+ + +K Q+CTQR KRP GVGLLV
Sbjct: 85 SRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCTQRSWKRPYGVGLLV 134
Score = 36.3 bits (80), Expect = 0.011
Identities = 13/28 (46%), Positives = 22/28 (78%)
Frame = +3
Query: 252 ISAYQKKIIPIDEHIGIXTPGLTAEARM 335
+S++Q+KI +D+HIG+ GLTA+ R+
Sbjct: 56 LSSHQRKIFKVDDHIGVAIAGLTADGRV 83
>At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2)
identical to 20S proteasome subunit PAE2 GB:AAC32061
from [Arabidopsis thaliana]
Length = 237
Score = 62.1 bits (144), Expect = 2e-10
Identities = 29/52 (55%), Positives = 36/52 (69%)
Frame = +1
Query: 94 RNQYDSDVTVWSPQGRLHQVEYAMEAVKLGSATIGLKNKDYAVLIALKRAVS 249
R +YD V +SP+GRL QVEYA+EA+KLGS IG+K K+ VL KR S
Sbjct: 5 RTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITS 56
Score = 28.7 bits (61), Expect = 2.2
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = +3
Query: 267 KKIIPIDEHIGIXTPGLTAEARMRV 341
+KI+ ID+HIG GL A+AR V
Sbjct: 65 EKIMEIDDHIGCAMSGLIADARTLV 89
Score = 27.5 bits (58), Expect = 5.2
Identities = 16/52 (30%), Positives = 25/52 (48%), Gaps = 3/52 (5%)
Frame = +2
Query: 344 YMRTECLNHRYSHDAPMPV---GRLISSVGNKMQICTQRYDKRPLGVGLLVA 490
+ R E NHR+S+ PM V + + + + + RP GV LL+A
Sbjct: 91 HARVETQNHRFSYGEPMTVESTTQALCDLALRFGEGEEESMSRPFGVSLLIA 142
>At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1)
identical to 20S proteasome subunit PAE1 GI:3421087 from
[Arabidopsis thaliana]
Length = 237
Score = 62.1 bits (144), Expect = 2e-10
Identities = 29/52 (55%), Positives = 36/52 (69%)
Frame = +1
Query: 94 RNQYDSDVTVWSPQGRLHQVEYAMEAVKLGSATIGLKNKDYAVLIALKRAVS 249
R +YD V +SP+GRL QVEYA+EA+KLGS IG+K K+ VL KR S
Sbjct: 5 RTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITS 56
Score = 28.7 bits (61), Expect = 2.2
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = +3
Query: 267 KKIIPIDEHIGIXTPGLTAEARMRV 341
+KI+ ID+HIG GL A+AR V
Sbjct: 65 EKIMEIDDHIGCAMSGLIADARTLV 89
Score = 27.5 bits (58), Expect = 5.2
Identities = 16/52 (30%), Positives = 25/52 (48%), Gaps = 3/52 (5%)
Frame = +2
Query: 344 YMRTECLNHRYSHDAPMPV---GRLISSVGNKMQICTQRYDKRPLGVGLLVA 490
+ R E NHR+S+ PM V + + + + + RP GV LL+A
Sbjct: 91 HARVETQNHRFSYGEPMTVESTTQALCDLALRFGEGEEESMSRPFGVSLLIA 142
>At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1)
(PRC9) identical to GB:AAC32057 from [Arabidopsis
thaliana] (Genetics (1998) 149 (2), 677-692); identical
to cDNA proteasome subunit prc9 GI:2511583
Length = 250
Score = 57.6 bits (133), Expect = 4e-09
Identities = 26/51 (50%), Positives = 37/51 (72%)
Frame = +1
Query: 100 QYDSDVTVWSPQGRLHQVEYAMEAVKLGSATIGLKNKDYAVLIALKRAVSE 252
+YDS T++SP+GRL+QVEYAMEA+ + IG+ +KD VLI K+ S+
Sbjct: 4 RYDSRTTIFSPEGRLYQVEYAMEAIGNAGSAIGILSKDGVVLIGEKKVTSK 54
Score = 31.5 bits (68), Expect = 0.32
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = +2
Query: 350 RTECLNHRYSHDAPMPVGRLISSVGNKMQICTQRYDKRPLGVGLLVA 490
R + + + + PMPV +L+ S+ + Q TQ RP GV L A
Sbjct: 91 RVQAQRYTFMYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFA 137
>At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2)
(PRS1) (PRC6) identical to SP|O24616 Proteasome subunit
alpha type 7-2 (EC 3.4.25.1) (20S proteasome alpha
subunit D2) {Arabidopsis thaliana}
Length = 250
Score = 54.8 bits (126), Expect = 3e-08
Identities = 28/66 (42%), Positives = 41/66 (62%), Gaps = 2/66 (3%)
Frame = +1
Query: 100 QYDSDVTVWSPQGRLHQVEYAMEAVKLGSATIGLKNKDYAVLIALKRAVSEFPHIR--RK 273
+YD +TV+SP G L QVEYA+EAV+ G+A +G++ D VL K++ + R RK
Sbjct: 3 RYDRAITVFSPDGHLFQVEYALEAVRKGNAAVGVRGTDTVVLAVEKKSTPKLQDSRSARK 62
Query: 274 LFLLMN 291
+ L N
Sbjct: 63 IVSLDN 68
Score = 28.7 bits (61), Expect = 2.2
Identities = 14/46 (30%), Positives = 22/46 (47%)
Frame = +2
Query: 350 RTECLNHRYSHDAPMPVGRLISSVGNKMQICTQRYDKRPLGVGLLV 487
R EC +HR + + P+ V + + Q TQ RP G+ L+
Sbjct: 89 RIECQSHRLTLEDPVTVEYITRYIAGLQQKYTQSGGVRPFGLSTLI 134
Score = 27.5 bits (58), Expect = 5.2
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = +3
Query: 267 KKIIPIDEHIGIXTPGLTAEARMRV 341
+KI+ +D HI + GL A+AR+ +
Sbjct: 61 RKIVSLDNHIALACAGLKADARVLI 85
>At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1)
Length = 250
Score = 54.8 bits (126), Expect = 3e-08
Identities = 28/66 (42%), Positives = 41/66 (62%), Gaps = 2/66 (3%)
Frame = +1
Query: 100 QYDSDVTVWSPQGRLHQVEYAMEAVKLGSATIGLKNKDYAVLIALKRAVSEFPHIR--RK 273
+YD +TV+SP G L QVEYA+EAV+ G+A +G++ D VL K++ + R RK
Sbjct: 3 RYDRAITVFSPDGHLFQVEYALEAVRKGNAAVGVRGTDTVVLAVEKKSTPKLQDSRSARK 62
Query: 274 LFLLMN 291
+ L N
Sbjct: 63 IVSLDN 68
Score = 28.7 bits (61), Expect = 2.2
Identities = 14/46 (30%), Positives = 22/46 (47%)
Frame = +2
Query: 350 RTECLNHRYSHDAPMPVGRLISSVGNKMQICTQRYDKRPLGVGLLV 487
R EC +HR + + P+ V + + Q TQ RP G+ L+
Sbjct: 89 RIECQSHRLTLEDPVTVEYITRYIAGLQQKYTQSGGVRPFGLSTLI 134
Score = 27.5 bits (58), Expect = 5.2
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = +3
Query: 267 KKIIPIDEHIGIXTPGLTAEARMRV 341
+KI+ +D HI + GL A+AR+ +
Sbjct: 61 RKIVSLDNHIALACAGLKADARVLI 85
>At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1)
(PRC1) identical to proteasome subunit alpha type 6-1
SP:O81146 GI:12643647 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc1 GI:2511587
Length = 246
Score = 49.2 bits (112), Expect = 1e-06
Identities = 22/47 (46%), Positives = 33/47 (70%), Gaps = 1/47 (2%)
Frame = +1
Query: 103 YDSDVTVWSPQGRLHQVEYAMEAVK-LGSATIGLKNKDYAVLIALKR 240
YD +T++SP+GRL QVEYA +AVK G +IG++ KD ++ K+
Sbjct: 9 YDRHITIFSPEGRLFQVEYAFKAVKTAGITSIGVRGKDSVCVVTQKK 55
Score = 32.3 bits (70), Expect = 0.18
Identities = 16/46 (34%), Positives = 24/46 (52%)
Frame = +2
Query: 350 RTECLNHRYSHDAPMPVGRLISSVGNKMQICTQRYDKRPLGVGLLV 487
R + R+++ MPV L + +K Q+ TQ RPLGV +V
Sbjct: 95 RNQAAEFRFTYGYEMPVDILAKWIADKSQVYTQHAYMRPLGVVAMV 140
>At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2)
identical to GB:AF043519
Length = 246
Score = 49.2 bits (112), Expect = 1e-06
Identities = 22/47 (46%), Positives = 33/47 (70%), Gaps = 1/47 (2%)
Frame = +1
Query: 103 YDSDVTVWSPQGRLHQVEYAMEAVK-LGSATIGLKNKDYAVLIALKR 240
YD +T++SP+GRL QVEYA +AVK G +IG++ KD ++ K+
Sbjct: 9 YDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSVCVVTQKK 55
Score = 31.5 bits (68), Expect = 0.32
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = +2
Query: 350 RTECLNHRYSHDAPMPVGRLISSVGNKMQICTQRYDKRPLGVGLLV 487
R E R+ + MP L + +K Q+ TQ RPLGV +V
Sbjct: 95 RNEAAEFRFQYGYEMPADILAKWIADKSQVYTQHAYMRPLGVVAMV 140
>At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1)
(PRC8) identical to proteasome subunit alpha type 3
SP:O23715, GI:12644056 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc8 GI:2511591
Length = 249
Score = 43.2 bits (97), Expect = 1e-04
Identities = 19/41 (46%), Positives = 26/41 (63%)
Frame = +1
Query: 103 YDSDVTVWSPQGRLHQVEYAMEAVKLGSATIGLKNKDYAVL 225
YD VT +SP GR+ Q+EYA +AV +G+K KD V+
Sbjct: 8 YDLSVTTFSPDGRVFQIEYAAKAVDNSGTVVGIKCKDGIVM 48
>At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putative
nearly identical to SP|O23708 Proteasome subunit alpha
type 2 (EC 3.4.25.1) (20S proteasome alpha subunit B)
{Arabidopsis thaliana} and to At1g16470
Length = 235
Score = 40.3 bits (90), Expect = 7e-04
Identities = 18/51 (35%), Positives = 31/51 (60%)
Frame = +1
Query: 97 NQYDSDVTVWSPQGRLHQVEYAMEAVKLGSATIGLKNKDYAVLIALKRAVS 249
+QY +T +SP G+L Q+E+A+ AV G ++G+K + V+ K+ S
Sbjct: 4 SQYSFSLTTFSPSGKLVQIEHALTAVGSGQTSLGIKASNGVVIATEKKLPS 54
Score = 31.9 bits (69), Expect = 0.24
Identities = 18/50 (36%), Positives = 24/50 (48%)
Frame = +2
Query: 341 RYMRTECLNHRYSHDAPMPVGRLISSVGNKMQICTQRYDKRPLGVGLLVA 490
R R + + + P+PV +L+ MQ TQ RP GV LLVA
Sbjct: 88 RKSRKQAEQYLRLYKEPIPVTQLVRETATVMQEFTQSGGVRPFGVSLLVA 137
>At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1)
(PRC3) identical to proteasome subunit alpha type 2
SP:O23708, GI:6093778; identical to cDNA proteasome
subunit prc3 GI:2511573
Length = 235
Score = 40.3 bits (90), Expect = 7e-04
Identities = 18/51 (35%), Positives = 31/51 (60%)
Frame = +1
Query: 97 NQYDSDVTVWSPQGRLHQVEYAMEAVKLGSATIGLKNKDYAVLIALKRAVS 249
+QY +T +SP G+L Q+E+A+ AV G ++G+K + V+ K+ S
Sbjct: 4 SQYSFSLTTFSPSGKLVQIEHALTAVGSGQTSLGIKASNGVVIATEKKLPS 54
Score = 31.9 bits (69), Expect = 0.24
Identities = 18/50 (36%), Positives = 24/50 (48%)
Frame = +2
Query: 341 RYMRTECLNHRYSHDAPMPVGRLISSVGNKMQICTQRYDKRPLGVGLLVA 490
R R + + + P+PV +L+ MQ TQ RP GV LLVA
Sbjct: 88 RKSRKQAEQYLRLYKEPIPVTQLVRETATVMQEFTQSGGVRPFGVSLLVA 137
>At3g09930.1 68416.m01188 GDSL-motif lipase/hydrolase family protein
similar to SP|P40602 Anther-specific proline-rich
protein APG precursor {Arabidopsis thaliana}; contains
Pfam profile: PF00657 lipase acylhydrolase with
GDSL-like motif
Length = 354
Score = 31.9 bits (69), Expect = 0.24
Identities = 15/55 (27%), Positives = 32/55 (58%), Gaps = 2/55 (3%)
Frame = +1
Query: 187 ATIGLKNKDYAVLIALKRAVSEFPHIRRKLF--LLMNILEXLHQVSLQKLVCESL 345
A + + DYA +ALKR ++E P +++ + +N + +H++ + K+V S+
Sbjct: 161 ALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIAVNAMR-IHKLGVNKIVIPSM 214
>At5g03610.1 68418.m00320 GDSL-motif lipase/hydrolase family protein
low similarity to SP|P40602 Anther-specific proline-rich
protein APG precursor {Arabidopsis thaliana}; contains
Pfam profile PF00657: GDSL-like Lipase/Acylhydrolase
Length = 359
Score = 30.7 bits (66), Expect = 0.56
Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +1
Query: 187 ATIGLKNKDYAVLIALKRAVSEFPHIRRKLFLLMNI-LEXLHQVSLQKLVCESL 345
A + + DY+ IAL R SEFP +++ + L +H + ++K+ SL
Sbjct: 164 ALVSVAGNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSL 217
>At4g24460.1 68417.m03506 expressed protein
Length = 431
Score = 27.9 bits (59), Expect = 3.9
Identities = 19/56 (33%), Positives = 30/56 (53%)
Frame = -3
Query: 436 LHFVTN*RDETSNWHWSIMRISVIETLCAHVATRIRASAVRPGVXIPICSSIGIIF 269
L VTN + W ++I I +E L VAT + A+A+ PG IPI + +++
Sbjct: 148 LGIVTNEMMDVPKWRFAI--IGFLEAL--GVATGMAAAAMLPGPVIPILNQTYLVW 199
>At4g31390.1 68417.m04452 ABC1 family protein contains Pfam domain,
PF03109: ABC1 family
Length = 682
Score = 27.1 bits (57), Expect = 6.9
Identities = 19/52 (36%), Positives = 27/52 (51%)
Frame = +1
Query: 187 ATIGLKNKDYAVLIALKRAVSEFPHIRRKLFLLMNILEXLHQVSLQKLVCES 342
AT+ +D A+ + +R E P I R LFL + L+ SLQKL C +
Sbjct: 253 ATLRATGEDVAIKV--QRPQIE-PIIYRDLFLFRTLASFLNGFSLQKLGCNA 301
>At4g00335.2 68417.m00044 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 190
Score = 27.1 bits (57), Expect = 6.9
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = -1
Query: 198 TNGCRTKFHSFHCILHLMETALRTPYCDVAIV 103
T C +FH C+L +E + R P CD +V
Sbjct: 154 TTKCEHEFH-LSCLLEWIERSDRCPICDKEVV 184
>At4g00335.1 68417.m00043 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 190
Score = 27.1 bits (57), Expect = 6.9
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = -1
Query: 198 TNGCRTKFHSFHCILHLMETALRTPYCDVAIV 103
T C +FH C+L +E + R P CD +V
Sbjct: 154 TTKCEHEFH-LSCLLEWIERSDRCPICDKEVV 184
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,722,201
Number of Sequences: 28952
Number of extensions: 209597
Number of successful extensions: 632
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 603
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 632
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 858708096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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