BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021950X (543 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g10380.1 68417.m01703 major intrinsic family protein / MIP fa... 29 2.7 At1g66180.1 68414.m07512 aspartyl protease family protein contai... 29 2.7 At3g12540.1 68416.m01560 expressed protein contains Pfam profile... 28 4.6 At2g02300.1 68415.m00168 F-box family protein / SKP1 interacting... 28 4.6 At4g09570.1 68417.m01575 calcium-dependent protein kinase, putat... 27 6.1 At5g03360.1 68418.m00289 DC1 domain-containing protein contains ... 27 8.1 >At4g10380.1 68417.m01703 major intrinsic family protein / MIP family protein contains Pfam profile: MIP PF00230 Length = 304 Score = 28.7 bits (61), Expect = 2.7 Identities = 12/34 (35%), Positives = 19/34 (55%), Gaps = 2/34 (5%) Frame = -1 Query: 423 PAPRCNTFSVMPMSYGPNNFCFDD--TSDIKLKK 328 P PRC VM ++G ++ CF D + D+ L + Sbjct: 45 PTPRCKCLPVMGSTWGQHDTCFTDFPSPDVSLTR 78 >At1g66180.1 68414.m07512 aspartyl protease family protein contains Pfam PF00026: Eukaryotic aspartyl protease profile; similar to CND41, chloroplast nucleoid DNA binding protein (GI:2541876) [Nicotiana tabacum] Length = 430 Score = 28.7 bits (61), Expect = 2.7 Identities = 20/61 (32%), Positives = 26/61 (42%), Gaps = 2/61 (3%) Frame = -1 Query: 537 TMSVYYWVRC--VTLPPKNRASRRSCLRGSSAAWKCSILTPAPRCNTFSVMPMSYGPNNF 364 T S W++C LPPK + S L S + CS PR F+ +P S N Sbjct: 91 TGSQLSWIQCHRKKLPPKPKTSFDPSLSSSFSTLPCSHPLCKPRIPDFT-LPTSCDSNRL 149 Query: 363 C 361 C Sbjct: 150 C 150 >At3g12540.1 68416.m01560 expressed protein contains Pfam profile PF04784: Protein of unknown function, DUF547 Length = 503 Score = 27.9 bits (59), Expect = 4.6 Identities = 20/50 (40%), Positives = 24/50 (48%) Frame = -1 Query: 459 GSSAAWKCSILTPAPRCNTFSVMPMSYGPNNFCFDDTSDIKLKKLVMVQS 310 GS W CS L PA RC M G N S+ +LKKL+ V+S Sbjct: 449 GSLIEWICSQLPPAQRCFQLKETAME-GLNK-----KSESQLKKLIEVRS 492 >At2g02300.1 68415.m00168 F-box family protein / SKP1 interacting partner 3-related contains similarity to SKP1 interacting partner 3 GI:10716951 from [Arabidopsis thaliana] Length = 284 Score = 27.9 bits (59), Expect = 4.6 Identities = 10/17 (58%), Positives = 13/17 (76%) Frame = +1 Query: 337 FDVRGIIETEIIRPITH 387 FD+RG++ T II P TH Sbjct: 168 FDIRGVLNTRIISPGTH 184 >At4g09570.1 68417.m01575 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium-dependent protein kinase [Arabidopsis thaliana] gi|604881|dbj|BAA04830; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 501 Score = 27.5 bits (58), Expect = 6.1 Identities = 15/36 (41%), Positives = 18/36 (50%), Gaps = 1/36 (2%) Frame = -1 Query: 435 SILTPAPRCNTFSVMPMSYGPNNFCFDDTS-DIKLK 331 +IL C++ VM P NF FD S D KLK Sbjct: 132 TILGVVEACHSLGVMHRDLKPENFLFDSPSDDAKLK 167 >At5g03360.1 68418.m00289 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 1610 Score = 27.1 bits (57), Expect = 8.1 Identities = 13/31 (41%), Positives = 16/31 (51%), Gaps = 1/31 (3%) Frame = -3 Query: 538 HDEC-LLLGPLCDFAA*ESCFETLLLARIQR 449 H C + P CDF A +SCF + RI R Sbjct: 299 HTRCPFYICPPCDFVAHQSCFSLPRVIRISR 329 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,933,728 Number of Sequences: 28952 Number of extensions: 245339 Number of successful extensions: 613 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 591 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 613 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1013649368 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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