BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021896 (510 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g05580.1 68418.m00606 omega-3 fatty acid desaturase, chloropl... 31 0.34 At3g11170.1 68416.m01355 omega-3 fatty acid desaturase, chloropl... 30 1.0 At1g01225.1 68414.m00037 NC domain-containing protein-related co... 29 1.8 At1g18370.1 68414.m02295 kinesin motor family protein (NACK1) si... 27 7.3 At3g18215.1 68416.m02317 expressed protein contains Pfam profile... 27 9.7 At1g52750.1 68414.m05963 hydrolase, alpha/beta fold family prote... 27 9.7 >At5g05580.1 68418.m00606 omega-3 fatty acid desaturase, chloroplast, temperature-sensitive (FAD8) identical to SP:48622 Temperature-sensitive omega-3 fatty acid desaturase, chloroplast precursor (EC 1.14.19.-) {Arabidopsis thaliana}; contains Pfam profile PF00487: Fatty acid desaturase; identical to cDNA plastid fatty acid desaturase GI:1030694 Length = 435 Score = 31.5 bits (68), Expect = 0.34 Identities = 13/26 (50%), Positives = 17/26 (65%) Frame = -3 Query: 385 HLVAATEVVESVAGQLFRRQKRNGPL 308 HLV ATE + V G+ +R K +GPL Sbjct: 372 HLVEATEAAKPVLGKYYREPKNSGPL 397 >At3g11170.1 68416.m01355 omega-3 fatty acid desaturase, chloroplast (FAD7) (FADD) identical to omega-3 fatty acid desaturase, chloroplast precursor SP:P46310 [Arabidopsis thaliana (Mouse-ear cress)]; identical to Pfam profile PF00487: Fatty acid desaturase; identical to cDNA plastid fatty acid desaturase GI:809491 Length = 446 Score = 29.9 bits (64), Expect = 1.0 Identities = 12/26 (46%), Positives = 17/26 (65%) Frame = -3 Query: 385 HLVAATEVVESVAGQLFRRQKRNGPL 308 HLV ATE + V G+ +R ++GPL Sbjct: 379 HLVEATEAAKPVLGKYYREPDKSGPL 404 >At1g01225.1 68414.m00037 NC domain-containing protein-related contains weak hit to Pfam profile PF04970: NC domain Length = 260 Score = 29.1 bits (62), Expect = 1.8 Identities = 18/75 (24%), Positives = 37/75 (49%), Gaps = 3/75 (4%) Frame = -3 Query: 499 GIHLFSYKGTLGRPSPMI---GSRIVAFDPPHDFYLTESTAHLVAATEVVESVAGQLFRR 329 G H+++Y+ I GS++V F P H+ + +++ +++E + S+ R Sbjct: 16 GDHIYTYRAIFAYSHHGIFVGGSKVVHFRPEHNPMDSSTSSISSSSSEDICSIFPDCGFR 75 Query: 328 QKRNGPLIKCFQTFL 284 Q +G ++ C FL Sbjct: 76 QPDSGVVLSCLDCFL 90 >At1g18370.1 68414.m02295 kinesin motor family protein (NACK1) similar to kinesin heavy chain isolog GB:AAB63609 GI:2262101 from [Arabidopsis thaliana] Length = 974 Score = 27.1 bits (57), Expect = 7.3 Identities = 16/50 (32%), Positives = 27/50 (54%), Gaps = 2/50 (4%) Frame = +2 Query: 284 EERLKTFDQWPVTFLTPEQLARNGFYYLGRGDEVCCAFCKVEIMR--WVE 427 EER + + W V ++ + R FY L +GD+ + +VE+ R W+E Sbjct: 794 EERQQIIELWHVCHVSI--IHRTQFYLLFKGDQADQIYMEVELRRLTWLE 841 >At3g18215.1 68416.m02317 expressed protein contains Pfam profile PF04654: Protein of unknown function, DUF599 Length = 244 Score = 26.6 bits (56), Expect = 9.7 Identities = 15/52 (28%), Positives = 25/52 (48%) Frame = +3 Query: 333 RNNWPATDSTTSVAATRCAVLSVR*KS*GGSKATILLPIIGDGRPSVPLYEN 488 RNN A+ + A T C+++ V + SK+T I G P + ++N Sbjct: 69 RNNIMASTLLATTAITLCSIIGVFVSNSSSSKSTATNLIYGSKSPRLASFKN 120 >At1g52750.1 68414.m05963 hydrolase, alpha/beta fold family protein contains Pfam profile PF00561: hydrolase, alpha/beta fold family Length = 633 Score = 26.6 bits (56), Expect = 9.7 Identities = 10/25 (40%), Positives = 17/25 (68%) Frame = -3 Query: 490 LFSYKGTLGRPSPMIGSRIVAFDPP 416 +FS++ +G S +G R+VA+D P Sbjct: 366 VFSWRHVMGELSLQLGCRVVAYDRP 390 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,672,223 Number of Sequences: 28952 Number of extensions: 200062 Number of successful extensions: 420 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 419 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 420 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 917929344 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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