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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021896
         (510 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g05580.1 68418.m00606 omega-3 fatty acid desaturase, chloropl...    31   0.34 
At3g11170.1 68416.m01355 omega-3 fatty acid desaturase, chloropl...    30   1.0  
At1g01225.1 68414.m00037 NC domain-containing protein-related co...    29   1.8  
At1g18370.1 68414.m02295 kinesin motor family protein (NACK1) si...    27   7.3  
At3g18215.1 68416.m02317 expressed protein contains Pfam profile...    27   9.7  
At1g52750.1 68414.m05963 hydrolase, alpha/beta fold family prote...    27   9.7  

>At5g05580.1 68418.m00606 omega-3 fatty acid desaturase,
           chloroplast, temperature-sensitive (FAD8) identical to
           SP:48622 Temperature-sensitive omega-3 fatty acid
           desaturase, chloroplast precursor (EC 1.14.19.-)
           {Arabidopsis thaliana}; contains Pfam profile PF00487:
           Fatty acid desaturase; identical to cDNA plastid fatty
           acid desaturase GI:1030694
          Length = 435

 Score = 31.5 bits (68), Expect = 0.34
 Identities = 13/26 (50%), Positives = 17/26 (65%)
 Frame = -3

Query: 385 HLVAATEVVESVAGQLFRRQKRNGPL 308
           HLV ATE  + V G+ +R  K +GPL
Sbjct: 372 HLVEATEAAKPVLGKYYREPKNSGPL 397


>At3g11170.1 68416.m01355 omega-3 fatty acid desaturase, chloroplast
           (FAD7) (FADD) identical to omega-3 fatty acid
           desaturase, chloroplast precursor SP:P46310 [Arabidopsis
           thaliana (Mouse-ear cress)]; identical to Pfam profile
           PF00487: Fatty acid desaturase; identical to cDNA
           plastid fatty acid desaturase GI:809491
          Length = 446

 Score = 29.9 bits (64), Expect = 1.0
 Identities = 12/26 (46%), Positives = 17/26 (65%)
 Frame = -3

Query: 385 HLVAATEVVESVAGQLFRRQKRNGPL 308
           HLV ATE  + V G+ +R   ++GPL
Sbjct: 379 HLVEATEAAKPVLGKYYREPDKSGPL 404


>At1g01225.1 68414.m00037 NC domain-containing protein-related
           contains weak hit to Pfam profile PF04970: NC domain
          Length = 260

 Score = 29.1 bits (62), Expect = 1.8
 Identities = 18/75 (24%), Positives = 37/75 (49%), Gaps = 3/75 (4%)
 Frame = -3

Query: 499 GIHLFSYKGTLGRPSPMI---GSRIVAFDPPHDFYLTESTAHLVAATEVVESVAGQLFRR 329
           G H+++Y+         I   GS++V F P H+   + +++   +++E + S+      R
Sbjct: 16  GDHIYTYRAIFAYSHHGIFVGGSKVVHFRPEHNPMDSSTSSISSSSSEDICSIFPDCGFR 75

Query: 328 QKRNGPLIKCFQTFL 284
           Q  +G ++ C   FL
Sbjct: 76  QPDSGVVLSCLDCFL 90


>At1g18370.1 68414.m02295 kinesin motor family protein (NACK1)
           similar to kinesin heavy chain isolog GB:AAB63609
           GI:2262101 from [Arabidopsis thaliana]
          Length = 974

 Score = 27.1 bits (57), Expect = 7.3
 Identities = 16/50 (32%), Positives = 27/50 (54%), Gaps = 2/50 (4%)
 Frame = +2

Query: 284 EERLKTFDQWPVTFLTPEQLARNGFYYLGRGDEVCCAFCKVEIMR--WVE 427
           EER +  + W V  ++   + R  FY L +GD+    + +VE+ R  W+E
Sbjct: 794 EERQQIIELWHVCHVSI--IHRTQFYLLFKGDQADQIYMEVELRRLTWLE 841


>At3g18215.1 68416.m02317 expressed protein contains Pfam profile
           PF04654: Protein of unknown function, DUF599
          Length = 244

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 15/52 (28%), Positives = 25/52 (48%)
 Frame = +3

Query: 333 RNNWPATDSTTSVAATRCAVLSVR*KS*GGSKATILLPIIGDGRPSVPLYEN 488
           RNN  A+    + A T C+++ V   +   SK+T    I G   P +  ++N
Sbjct: 69  RNNIMASTLLATTAITLCSIIGVFVSNSSSSKSTATNLIYGSKSPRLASFKN 120


>At1g52750.1 68414.m05963 hydrolase, alpha/beta fold family protein
           contains Pfam profile PF00561: hydrolase, alpha/beta
           fold family
          Length = 633

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 10/25 (40%), Positives = 17/25 (68%)
 Frame = -3

Query: 490 LFSYKGTLGRPSPMIGSRIVAFDPP 416
           +FS++  +G  S  +G R+VA+D P
Sbjct: 366 VFSWRHVMGELSLQLGCRVVAYDRP 390


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,672,223
Number of Sequences: 28952
Number of extensions: 200062
Number of successful extensions: 420
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 419
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 420
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 917929344
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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