BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021883 (654 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 71 1e-13 SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 59 6e-10 SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|... 28 1.0 SPAC6C3.02c |||CHCH domain protein|Schizosaccharomyces pombe|chr... 27 1.8 SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 27 1.8 SPAC31G5.08 |ups1|ups|uroporphyrinogen-III synthase Ups1|Schizos... 27 2.4 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 3.1 SPAC22H10.02 |||conserved fungal protein|Schizosaccharomyces pom... 26 4.1 SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosa... 26 4.1 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 5.5 SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 26 5.5 SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizo... 26 5.5 SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 7.2 SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schi... 25 7.2 SPAC3A12.16c |tim17||TIM23 translocase complex subunit Tim17|Sch... 25 7.2 SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 25 7.2 SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 25 7.2 SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces... 25 7.2 SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 25 9.5 SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 25 9.5 SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomy... 25 9.5 >SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 407 Score = 70.9 bits (166), Expect = 1e-13 Identities = 32/65 (49%), Positives = 46/65 (70%) Frame = +2 Query: 251 SASKDIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAILRAM 430 S DIK+LLLGAG+SGK+TI+KQM++++ GF+ KQYR +++ N I SL +L AM Sbjct: 70 SGGNDIKVLLLGAGDSGKTTIMKQMRLLYSPGFSQVVRKQYRVMIFENIISSLCLLLEAM 129 Query: 431 PNLGI 445 N + Sbjct: 130 DNSNV 134 >SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 58.8 bits (136), Expect = 6e-10 Identities = 31/92 (33%), Positives = 57/92 (61%) Frame = +2 Query: 170 GCAQSAEERAADCSKQIDR*ELEREWYSASKDIKLLLLGAGESGKSTIVKQMKIIHESGF 349 G ++S E + + +I++ ++E K K+LLLGA +SGKSTI KQ+KI++++GF Sbjct: 6 GLSESGESKKLN--SKIEK-QIENASKKDKKIYKVLLLGASDSGKSTISKQIKILNKNGF 62 Query: 350 TNEDFKQYRPVVYSNTIQSLVAILRAMPNLGI 445 + E+ + PV+ N ++S +++ + GI Sbjct: 63 SQEEIMTFIPVIRRNLLESAKTLVKIIVQKGI 94 >SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr 1|||Manual Length = 1234 Score = 28.3 bits (60), Expect = 1.0 Identities = 15/27 (55%), Positives = 17/27 (62%) Frame = -2 Query: 245 ILFQVLIDQFASSSPLHALRPTGRTPY 165 ILF LI +SSS LH L P+G PY Sbjct: 183 ILFNALISILSSSSTLHKLYPSG-LPY 208 >SPAC6C3.02c |||CHCH domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 172 Score = 27.5 bits (58), Expect = 1.8 Identities = 14/36 (38%), Positives = 20/36 (55%), Gaps = 1/36 (2%) Frame = +2 Query: 107 SAPGSGVGAACGHSIGRTADMGCAQSAEERA-ADCS 211 +A G G+G+A GH++G G + S A AD S Sbjct: 64 TAAGVGIGSAIGHTVGSVITGGFSGSGSNNAPADTS 99 >SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 27.5 bits (58), Expect = 1.8 Identities = 9/26 (34%), Positives = 22/26 (84%) Frame = +2 Query: 254 ASKDIKLLLLGAGESGKSTIVKQMKI 331 ++K++++L+LG +GK+TI+ ++K+ Sbjct: 18 SNKEMRILMLGLDAAGKTTILYKLKL 43 >SPAC31G5.08 |ups1|ups|uroporphyrinogen-III synthase Ups1|Schizosaccharomyces pombe|chr 1|||Manual Length = 251 Score = 27.1 bits (57), Expect = 2.4 Identities = 21/67 (31%), Positives = 31/67 (46%) Frame = +2 Query: 125 VGAACGHSIGRTADMGCAQSAEERAADCSKQIDR*ELEREWYSASKDIKLLLLGAGESGK 304 VG A SI R +G Q+ DC + +L EWY+ +K K LL GE + Sbjct: 93 VGPATDDSIRR---LGFQQT---HGKDCGRGEVLADLIEEWYTTTKQHKPLLFLVGEKHR 146 Query: 305 STIVKQM 325 I +++ Sbjct: 147 DIIQRKL 153 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 26.6 bits (56), Expect = 3.1 Identities = 14/45 (31%), Positives = 20/45 (44%), Gaps = 2/45 (4%) Frame = -1 Query: 171 PISAVLPMLWPQAA--PTPEPGALSPISQHALYTRPVAPALPEPP 43 P S + P+ P A P P P P+ + ++ PV P PP Sbjct: 1174 PSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPP 1218 >SPAC22H10.02 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 158 Score = 26.2 bits (55), Expect = 4.1 Identities = 17/62 (27%), Positives = 25/62 (40%) Frame = -1 Query: 267 MSFDAEYHSLSSSYRSICFEQSAARSSADWAHPISAVLPMLWPQAAPTPEPGALSPISQH 88 M D E+ S + QS S + +S + P L+P P PG +S + H Sbjct: 55 MEDDLEFCPSLSDEELVSIYQSTGLSPTSSSPSLSPMTPNLYPNVLPNVHPG-VSNYTHH 113 Query: 87 AL 82 L Sbjct: 114 PL 115 >SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 26.2 bits (55), Expect = 4.1 Identities = 21/78 (26%), Positives = 30/78 (38%) Frame = -1 Query: 282 NNNSLMSFDAEYHSLSSSYRSICFEQSAARSSADWAHPISAVLPMLWPQAAPTPEPGALS 103 NNN+L + + SSS + S S P M Q++ E S Sbjct: 14 NNNALEKTSSNEATSSSSTQVSSLSASGISVSTPRVSPFEPERNMQ-VQSSQHLEANVQS 72 Query: 102 PISQHALYTRPVAPALPE 49 P+S Y P A A+P+ Sbjct: 73 PVSSQTTYATPSAYAVPQ 90 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 25.8 bits (54), Expect = 5.5 Identities = 14/39 (35%), Positives = 18/39 (46%), Gaps = 1/39 (2%) Frame = -1 Query: 156 LPMLWPQAAPTPEPGALS-PISQHALYTRPVAPALPEPP 43 LPM P A P P ++ P+ P+ PA P PP Sbjct: 439 LPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPP 477 >SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosaccharomyces pombe|chr 1|||Manual Length = 186 Score = 25.8 bits (54), Expect = 5.5 Identities = 9/20 (45%), Positives = 17/20 (85%) Frame = +2 Query: 260 KDIKLLLLGAGESGKSTIVK 319 +++++LLLG +GK+TI+K Sbjct: 15 REVRVLLLGLDNAGKTTILK 34 >SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizosaccharomyces pombe|chr 1|||Manual Length = 335 Score = 25.8 bits (54), Expect = 5.5 Identities = 15/38 (39%), Positives = 18/38 (47%), Gaps = 2/38 (5%) Frame = -1 Query: 129 PTPEPGALSPISQHALYTRPVAPALPEPPY--TILAPN 22 P P PG + A T P AP +P P+ TI A N Sbjct: 290 PPPPPGMVMASPSPAAATIPGAPVMPNIPFYQTINAQN 327 >SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 25.4 bits (53), Expect = 7.2 Identities = 13/36 (36%), Positives = 18/36 (50%) Frame = +2 Query: 251 SASKDIKLLLLGAGESGKSTIVKQMKIIHESGFTNE 358 S I + +G GKST+VK + +H F NE Sbjct: 19 SRQATINIGTIGHVAHGKSTVVKAISGVHTVRFKNE 54 >SPBP23A10.10 |ppk32||serine/threonine protein kinase Ppk32 |Schizosaccharomyces pombe|chr 2|||Manual Length = 749 Score = 25.4 bits (53), Expect = 7.2 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = +3 Query: 285 VLANRARVL*SNR*KLSMKVDSQMKTSNSTGPWS 386 +LAN R++ SN K+ D Q + S GPW+ Sbjct: 1 MLANALRLV-SNSKKIKYDYDIQKENSVQVGPWT 33 >SPAC3A12.16c |tim17||TIM23 translocase complex subunit Tim17|Schizosaccharomyces pombe|chr 1|||Manual Length = 164 Score = 25.4 bits (53), Expect = 7.2 Identities = 9/16 (56%), Positives = 12/16 (75%) Frame = -1 Query: 90 HALYTRPVAPALPEPP 43 +A Y RPVAP +P+ P Sbjct: 137 NAEYNRPVAPVIPDAP 152 >SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosaccharomyces pombe|chr 1|||Manual Length = 1168 Score = 25.4 bits (53), Expect = 7.2 Identities = 16/44 (36%), Positives = 28/44 (63%), Gaps = 1/44 (2%) Frame = +2 Query: 230 ELEREWYSA-SKDIKLLLLGAGESGKSTIVKQMKIIHESGFTNE 358 +L +++ A SK+ L+LLG SGK+T + Q + E G+T++ Sbjct: 514 KLRKQFLEAVSKNQILVLLGETGSGKTTQITQ--YLAEEGYTSD 555 >SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2|||Manual Length = 1217 Score = 25.4 bits (53), Expect = 7.2 Identities = 18/53 (33%), Positives = 22/53 (41%), Gaps = 2/53 (3%) Frame = -1 Query: 165 SAVLPMLWPQAAPTPEPGALSPISQHALYTRPVAPALPEPPYT--ILAPNTRT 13 +A P+ P A P P A + RP APA+ P T AP T T Sbjct: 995 AATKPVSMPAAKSKPAPMANPVSTAQQTQNRPPAPAMQARPNTTQAAAPVTST 1047 >SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces pombe|chr 2|||Manual Length = 466 Score = 25.4 bits (53), Expect = 7.2 Identities = 19/85 (22%), Positives = 39/85 (45%) Frame = -1 Query: 297 DSPAPNNNSLMSFDAEYHSLSSSYRSICFEQSAARSSADWAHPISAVLPMLWPQAAPTPE 118 D P P + ++ ++Y + S+ Y+++ + ++A +P +A +P P+ Sbjct: 320 DIPPPYSTPSVAGASDYSTPSAGYQTVQTTTTTTEAAAA-QYPQAA-----FPPPPVMPQ 373 Query: 117 PGALSPISQHALYTRPVAPALPEPP 43 P A + + A A A+P PP Sbjct: 374 PAAAAVTTPVAAPVAAAAAAVPVPP 398 >SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1064 Score = 25.0 bits (52), Expect = 9.5 Identities = 15/48 (31%), Positives = 27/48 (56%), Gaps = 1/48 (2%) Frame = +2 Query: 344 GFTNEDFKQYRPVVYSNTIQSLVAILRAMPNLGIIYGNRD-RESDGKM 484 GF +E+ KQYR +Y+ + L+ ++ M N I + R + DG++ Sbjct: 701 GFVDEEMKQYR--LYT-VVPQLLGLIEEMTNWYIRFNRRRLKGEDGEI 745 >SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccharomyces pombe|chr 2|||Manual Length = 884 Score = 25.0 bits (52), Expect = 9.5 Identities = 14/43 (32%), Positives = 20/43 (46%), Gaps = 4/43 (9%) Frame = -1 Query: 153 PMLWPQAAPTPEPGALSPISQHALYTRPV----APALPEPPYT 37 P + PQ P P A +P +H PV +P++P P T Sbjct: 721 PAIKPQVTPAPPTPAPTPAVKHHPPPPPVRSSISPSMPPAPLT 763 >SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 991 Score = 25.0 bits (52), Expect = 9.5 Identities = 7/13 (53%), Positives = 10/13 (76%) Frame = -2 Query: 461 LYCHR*CPSWAWH 423 LY ++ CPSW W+ Sbjct: 626 LYYYQGCPSWTWY 638 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,712,315 Number of Sequences: 5004 Number of extensions: 55921 Number of successful extensions: 221 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 198 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 218 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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