Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021883
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g26300.1 68415.m03156 guanine nucleotide binding protein (G-p... 62 3e-10
At1g31930.2 68414.m03924 extra-large guanine nucleotide binding ... 39 0.003
At1g31930.1 68414.m03923 extra-large guanine nucleotide binding ... 39 0.003
At4g34390.1 68417.m04885 extra-large guanine nucleotide binding ... 37 0.010
At5g64020.1 68418.m08038 expressed protein strong similarity to ... 31 0.51
At5g26070.1 68418.m03102 hydroxyproline-rich glycoprotein family... 31 0.67
At1g56130.1 68414.m06445 leucine-rich repeat family protein / pr... 31 0.88
At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibit... 30 1.2
At5g56200.1 68418.m07012 zinc finger (C2H2 type) family protein ... 30 1.5
At5g28840.1 68418.m03547 NAD-dependent epimerase/dehydratase fam... 29 2.0
At5g51300.2 68418.m06360 splicing factor-related contains simila... 29 3.6
At5g51300.1 68418.m06359 splicing factor-related contains simila... 29 3.6
At1g56140.1 68414.m06446 leucine-rich repeat family protein / pr... 29 3.6
At1g56120.1 68414.m06444 leucine-rich repeat family protein / pr... 29 3.6
At1g26150.1 68414.m03192 protein kinase family protein similar t... 28 4.7
At4g13340.1 68417.m02084 leucine-rich repeat family protein / ex... 28 6.2
At1g68725.1 68414.m07853 arabinogalactan-protein, putative (AGP1... 28 6.2
At5g56890.1 68418.m07099 protein kinase family protein contains ... 27 8.2
At4g24650.1 68417.m03529 adenylate isopentenyltransferase 4 / cy... 27 8.2
At3g32150.1 68416.m04094 hypothetical protein 27 8.2
At3g02670.1 68416.m00258 proline-rich family protein contains pr... 27 8.2
At2g34870.1 68415.m04281 hydroxyproline-rich glycoprotein family... 27 8.2
At2g18470.1 68415.m02151 protein kinase family protein contains ... 27 8.2
At1g12040.1 68414.m01390 leucine-rich repeat family protein / ex... 27 8.2
>At2g26300.1 68415.m03156 guanine nucleotide binding protein
(G-protein) alpha-1 subunit / GP-alpha-1 (GPA1)
identical to SP|P18064 Guanine nucleotide-binding
protein alpha-1 subunit (GP-alpha-1) {Arabidopsis
thaliana}
Length = 383
Score = 62.1 bits (144), Expect = 3e-10
Identities = 30/65 (46%), Positives = 46/65 (70%)
Frame = +2
Query: 215 QIDR*ELEREWYSASKDIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSN 394
+I+R +E+E + KLLLLGAGESGKSTI KQ+K++ ++GF + K Y PV+++N
Sbjct: 23 EIER-RIEQEAKAEKHIRKLLLLGAGESGKSTIFKQIKLLFQTGFDEGELKSYVPVIHAN 81
Query: 395 TIQSL 409
Q++
Sbjct: 82 VYQTI 86
Score = 38.7 bits (86), Expect = 0.003
Identities = 16/48 (33%), Positives = 26/48 (54%)
Frame = +1
Query: 511 DTEPFSEELLAAMKRLWADSGVQECFGRSNEYQLNDSAKYFLNDLDRL 654
D ++++ ++ LW D +QE R NE Q+ D KY + +L RL
Sbjct: 127 DYPRLTKDIAEGIETLWKDPAIQETCARGNELQVPDCTKYLMENLKRL 174
>At1g31930.2 68414.m03924 extra-large guanine nucleotide binding
protein, putative / G-protein, putative similar to
extra-large G-protein (XLG) [Arabidopsis thaliana]
GI:3201680; contains Pfam profile PF00503: G-protein
alpha subunit
Length = 848
Score = 38.7 bits (86), Expect = 0.003
Identities = 21/51 (41%), Positives = 31/51 (60%)
Frame = +2
Query: 269 KLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAIL 421
KLLLLG SG STI KQ K ++ + F+ E+ + + +V SN + L +L
Sbjct: 432 KLLLLGIEGSGTSTIFKQAKFLYGNKFSVEELQDIKLMVQSNMYRYLSILL 482
>At1g31930.1 68414.m03923 extra-large guanine nucleotide binding
protein, putative / G-protein, putative similar to
extra-large G-protein (XLG) [Arabidopsis thaliana]
GI:3201680; contains Pfam profile PF00503: G-protein
alpha subunit
Length = 848
Score = 38.7 bits (86), Expect = 0.003
Identities = 21/51 (41%), Positives = 31/51 (60%)
Frame = +2
Query: 269 KLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAIL 421
KLLLLG SG STI KQ K ++ + F+ E+ + + +V SN + L +L
Sbjct: 432 KLLLLGIEGSGTSTIFKQAKFLYGNKFSVEELQDIKLMVQSNMYRYLSILL 482
>At4g34390.1 68417.m04885 extra-large guanine nucleotide binding
protein, putative / G-protein, putative similar to
extra-large G-protein (XLG) [Arabidopsis thaliana]
GI:3201680; contains Pfam profile PF00503: G-protein
alpha subunit
Length = 861
Score = 37.1 bits (82), Expect = 0.010
Identities = 17/53 (32%), Positives = 32/53 (60%)
Frame = +2
Query: 269 KLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAILRA 427
KLLL+G+ + G +TI KQ + ++ F+ ED ++ + ++ +N L +L A
Sbjct: 464 KLLLIGSEKGGATTIYKQARSLYNVSFSLEDRERIKFIIQTNLYTYLAMVLEA 516
>At5g64020.1 68418.m08038 expressed protein strong similarity to
unknown protein (pir||T02538)
Length = 408
Score = 31.5 bits (68), Expect = 0.51
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = -1
Query: 342 LSWIIFICLTIVLLPDSPAPNNNSLMSFDAEYHSLSSSYRSIC 214
L+ I+ I L I+LL P +SL ++ S SSS S+C
Sbjct: 15 LALIVLILLVIILLVSEENPLRDSLFEVKRQFSSSSSSSSSVC 57
>At5g26070.1 68418.m03102 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965; similar to root nodule extensin
[Pisum sativum] gi|15021750|gb|AAK77902; Common family
members: At5g19800, At5g57070, At1g72790 [Arabidopsis
thaliana]
Length = 102
Score = 31.1 bits (67), Expect = 0.67
Identities = 16/38 (42%), Positives = 19/38 (50%), Gaps = 2/38 (5%)
Frame = -1
Query: 153 PMLWPQAAPTPEPGALSPISQHALYTRPVA--PALPEP 46
P+ P +P P L P H Y+RPVA P LP P
Sbjct: 38 PLYSPSPSPYRSPVTLPPPPPHPAYSRPVALPPTLPIP 75
>At1g56130.1 68414.m06445 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 1032
Score = 30.7 bits (66), Expect = 0.88
Identities = 22/62 (35%), Positives = 27/62 (43%)
Frame = +1
Query: 463 PRKRW*DGVRRDSTDEDTEPFSEELLAAMKRLWADSGVQECFGRSNEYQLNDSAKYFLND 642
P KR G + DED P S + A + WA S V G SN + S F+N
Sbjct: 434 PEKRSVTGALFEREDEDFGPASFFVSAGQR--WAASSVGLFAGSSNNIYIATSQSQFVNT 491
Query: 643 LD 648
LD
Sbjct: 492 LD 493
>At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibitor
family protein low similarity to extensin [Volvox
carteri] GI:21992
Length = 312
Score = 30.3 bits (65), Expect = 1.2
Identities = 32/105 (30%), Positives = 51/105 (48%), Gaps = 8/105 (7%)
Frame = -1
Query: 294 SPAPNNNSLMSFDAEYHSLS-SSYRSICFEQSAARSSADWAHPISAVLPMLWPQ---AAP 127
SP+P ++S S A SLS SS + S+ + P+S++ P L P ++P
Sbjct: 37 SPSPPSSSPSS--APPSSLSPSSPPPLSLSPSSPPPPPPSSSPLSSLSPSLSPSPPSSSP 94
Query: 126 TPEP-GALSPISQHALYTRPVAPALPEP---PYTILAPNTRT*QY 4
+ P +LSP S L P +P P P P + L+P++ + Y
Sbjct: 95 SSAPPSSLSPSSPPPLSLSPSSPPPPPPSSSPLSSLSPSSSSSTY 139
>At5g56200.1 68418.m07012 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 493
Score = 29.9 bits (64), Expect = 1.5
Identities = 23/88 (26%), Positives = 35/88 (39%), Gaps = 2/88 (2%)
Frame = -1
Query: 279 NNSLMSFDAEYHSLSSSYRSICFEQS-AARSSADWAHPISAVLPMLWPQAAPT-PEPGAL 106
N SL S H + ++S Q+ W P+S V P P AAPT P +
Sbjct: 400 NTSLSSSHNGDHVCNICHKSFSTGQALGGHKRCHWTAPVSTVAPTTVPTAAPTVPATASS 459
Query: 105 SPISQHALYTRPVAPALPEPPYTILAPN 22
S +++ + + + E L PN
Sbjct: 460 SQVTETVQEVKKLKRRVLEFDLNELPPN 487
>At5g28840.1 68418.m03547 NAD-dependent epimerase/dehydratase family
protein similar to sugar epimerase BlmG from
Streptomyces verticillus GI:9937230; contains Pfam
profile PF01370 NAD dependent epimerase/dehydratase
family
Length = 377
Score = 29.5 bits (63), Expect = 2.0
Identities = 16/40 (40%), Positives = 25/40 (62%)
Frame = +2
Query: 230 ELEREWYSASKDIKLLLLGAGESGKSTIVKQMKIIHESGF 349
ELERE Y S+++K+ + GAG S I +++K HE +
Sbjct: 16 ELEREQYWPSENLKISITGAGGFIASHIARRLK--HEGHY 53
>At5g51300.2 68418.m06360 splicing factor-related contains
similarity to SF1 protein [Drosophila melanogaster]
GI:6687400
Length = 804
Score = 28.7 bits (61), Expect = 3.6
Identities = 14/43 (32%), Positives = 18/43 (41%)
Frame = -1
Query: 171 PISAVLPMLWPQAAPTPEPGALSPISQHALYTRPVAPALPEPP 43
P +P P A P P PG+ P+ H + P P PP
Sbjct: 604 PWGPPVPSYSPYALPPPPPGSYHPV--HGQHMPPYGMQYPPPP 644
>At5g51300.1 68418.m06359 splicing factor-related contains
similarity to SF1 protein [Drosophila melanogaster]
GI:6687400
Length = 804
Score = 28.7 bits (61), Expect = 3.6
Identities = 14/43 (32%), Positives = 18/43 (41%)
Frame = -1
Query: 171 PISAVLPMLWPQAAPTPEPGALSPISQHALYTRPVAPALPEPP 43
P +P P A P P PG+ P+ H + P P PP
Sbjct: 604 PWGPPVPSYSPYALPPPPPGSYHPV--HGQHMPPYGMQYPPPP 644
>At1g56140.1 68414.m06446 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 1032
Score = 28.7 bits (61), Expect = 3.6
Identities = 18/48 (37%), Positives = 23/48 (47%)
Frame = +1
Query: 505 DEDTEPFSEELLAAMKRLWADSGVQECFGRSNEYQLNDSAKYFLNDLD 648
DED P S + A + WA S V G SN ++ S F+N LD
Sbjct: 446 DEDLGPASFVVSAGQR--WAASSVGLFAGSSNNIYISTSQSQFVNTLD 491
>At1g56120.1 68414.m06444 leucine-rich repeat family protein /
protein kinase family protein contains Pfam domains
PF00560: Leucine Rich Repeat and PF00069: Protein kinase
domain
Length = 1045
Score = 28.7 bits (61), Expect = 3.6
Identities = 21/62 (33%), Positives = 28/62 (45%)
Frame = +1
Query: 463 PRKRW*DGVRRDSTDEDTEPFSEELLAAMKRLWADSGVQECFGRSNEYQLNDSAKYFLND 642
P R G + D D P S +++A KR WA S V G SN + S F+N
Sbjct: 450 PEIRSVSGALFEKEDADLGPASF-VVSAAKR-WAASSVGNFAGSSNNIYIATSLAQFINT 507
Query: 643 LD 648
+D
Sbjct: 508 MD 509
>At1g26150.1 68414.m03192 protein kinase family protein similar to
Pto kinase interactor 1 GI:3668069 from [Lycopersicon
esculentum]
Length = 760
Score = 28.3 bits (60), Expect = 4.7
Identities = 17/52 (32%), Positives = 26/52 (50%)
Frame = -1
Query: 177 AHPISAVLPMLWPQAAPTPEPGALSPISQHALYTRPVAPALPEPPYTILAPN 22
A+P+S+ P P P E +PI+ + T P P PE P ++ AP+
Sbjct: 112 ANPVSSPPPESSPPPPPPTEAPPTTPITSPSPPTNPPPP--PESPPSLPAPD 161
>At4g13340.1 68417.m02084 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 760
Score = 27.9 bits (59), Expect = 6.2
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = -1
Query: 153 PMLWPQAAPTPEPGALSPISQHALYTRPVAPALPEPP 43
P ++P +P P P +S +Y+ P P EPP
Sbjct: 583 PPIYPYLSPPPPPTPVSSPPPTPVYSPPPPPPCIEPP 619
>At1g68725.1 68414.m07853 arabinogalactan-protein, putative (AGP19)
non-consensus splice site at the intron:exon boundary
(AT:exon)
Length = 247
Score = 27.9 bits (59), Expect = 6.2
Identities = 18/51 (35%), Positives = 24/51 (47%), Gaps = 2/51 (3%)
Frame = -1
Query: 171 PISAVLPMLWPQAAPTPEPGALSPISQHALYTRPVA--PALPEPPYTILAP 25
P V P++ P A P P+P P S + PV+ PA PP T +P
Sbjct: 83 PAPKVAPVISP-ATPPPQPPQSPPASAPTVSPPPVSPPPAPTSPPPTPASP 132
>At5g56890.1 68418.m07099 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 1113
Score = 27.5 bits (58), Expect = 8.2
Identities = 27/85 (31%), Positives = 37/85 (43%), Gaps = 2/85 (2%)
Frame = -1
Query: 309 VLLPDSPAPNNNSLMSFDAEYHSLSSSYRSICFEQSAARSSADWAHPISAVLPMLWPQAA 130
V LP P P+N + + H +SI S S D A P+ V+P L P ++
Sbjct: 167 VALPP-PTPSNVPPRNA-SNNHKPPPIEKSIAPVASPPTISIDIAPPVHPVIPKLTPSSS 224
Query: 129 PTP--EPGALSPISQHALYTRPVAP 61
P P P SP ++ T PV P
Sbjct: 225 PVPTSTPTKGSP-RRNPPTTHPVFP 248
>At4g24650.1 68417.m03529 adenylate isopentenyltransferase 4 /
cytokinin synthase (IPT4) identical to adenylate
isopentenyltransferase (IPT4) [Arabidopsis thaliana]
GI:14279062
Length = 318
Score = 27.5 bits (58), Expect = 8.2
Identities = 15/58 (25%), Positives = 30/58 (51%)
Frame = +2
Query: 263 DIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLVAILRAMPN 436
D ++++GA SGKS++ + + ++ N D Q+ + T QS + R +P+
Sbjct: 5 DKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPH 62
>At3g32150.1 68416.m04094 hypothetical protein
Length = 241
Score = 27.5 bits (58), Expect = 8.2
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = -1
Query: 198 ARSSADWAHPISAVLPMLWPQAAPTPEPGALSP 100
+ S DWA P V P + + TPEP + P
Sbjct: 150 SESGLDWAGPSEPVEPEISVTSVETPEPVVIPP 182
>At3g02670.1 68416.m00258 proline-rich family protein contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 217
Score = 27.5 bits (58), Expect = 8.2
Identities = 15/47 (31%), Positives = 19/47 (40%)
Frame = -1
Query: 156 LPMLWPQAAPTPEPGALSPISQHALYTRPVAPALPEPPYTILAPNTR 16
LP+ +P P PG P T P P PP L P+T+
Sbjct: 171 LPLPFPPLPPVTIPGLPLPFPPLPPVTIPSFPGFRFPPLPFLPPSTQ 217
>At2g34870.1 68415.m04281 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 116
Score = 27.5 bits (58), Expect = 8.2
Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = -1
Query: 156 LPMLWPQAAPTPEPGALSPIS-QHALYTRPVAPALPEPP 43
LP L+P P+P PG + + T P AP+LP P
Sbjct: 63 LPSLFPPFVPSPFPGNIPRLPFPFPFPTSPPAPSLPGFP 101
>At2g18470.1 68415.m02151 protein kinase family protein contains
Pfam PF00069: Protein kinase domain
Length = 633
Score = 27.5 bits (58), Expect = 8.2
Identities = 17/50 (34%), Positives = 21/50 (42%), Gaps = 3/50 (6%)
Frame = -1
Query: 144 WPQAAPTPEPGALSPIS--QHALYTRPVAPALPEP-PYTILAPNTRT*QY 4
W P+P P P S ++Y+ P P LP P P L N T Y
Sbjct: 225 WGGGGPSPPPPPRMPTSGEDSSMYSGPSRPVLPPPSPALALGFNKSTFTY 274
>At1g12040.1 68414.m01390 leucine-rich repeat family protein /
extensin family protein (LRX1) similar to extensin-like
protein [Lycopersicon esculentum]
gi|5917664|gb|AAD55979; contains leucine-rich repeats,
Pfam:PF00560; contains proline rich extensin domains,
INTERPRO:IPR002965
Length = 744
Score = 27.5 bits (58), Expect = 8.2
Identities = 17/43 (39%), Positives = 22/43 (51%)
Frame = -1
Query: 153 PMLWPQAAPTPEPGALSPISQHALYTRPVAPALPEPPYTILAP 25
P+ +PQ P+P P SP LY PV P+ P PP + P
Sbjct: 602 PVYYPQVTPSPPPP--SP-----LYYPPVTPS-PPPPSPVYYP 636
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,446,808
Number of Sequences: 28952
Number of extensions: 306817
Number of successful extensions: 1291
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 1141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1285
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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