Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021874
(674 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g24460.1 68414.m03081 myosin-related contains TIGRFAM TIGR016... 34 0.075
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 33 0.17
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 33 0.17
At4g38070.1 68417.m05377 bHLH family protein contains Pfam profi... 33 0.23
At1g12080.2 68414.m01397 expressed protein 33 0.23
At5g16730.1 68418.m01959 expressed protein weak similarity to mi... 30 1.2
At1g12080.1 68414.m01396 expressed protein 30 1.2
At2g07190.1 68415.m00824 hypothetical protein 30 1.6
At1g67230.1 68414.m07652 expressed protein 30 1.6
At2g18540.1 68415.m02160 cupin family protein contains Pfam prof... 29 2.1
At3g16000.1 68416.m02024 matrix-localized MAR DNA-binding protei... 29 2.8
At1g40133.1 68414.m04768 hypothetical protein 29 2.8
At5g28430.1 68418.m03453 hypothetical protein 29 3.8
At3g26420.1 68416.m03295 glycine-rich RNA-binding protein simila... 29 3.8
At3g05900.1 68416.m00664 neurofilament protein-related similar t... 28 6.6
At1g64330.1 68414.m07290 myosin heavy chain-related similar to m... 28 6.6
At5g61040.1 68418.m07660 expressed protein 27 8.7
At5g08780.1 68418.m01041 histone H1/H5 family protein contains P... 27 8.7
At4g17220.1 68417.m02590 expressed protein 27 8.7
At4g02510.1 68417.m00343 chloroplast outer membrane protein, put... 27 8.7
At3g06020.1 68416.m00687 expressed protein ; expression supporte... 27 8.7
At2g37080.1 68415.m04550 myosin heavy chain-related low similari... 27 8.7
At2g31130.1 68415.m03801 expressed protein 27 8.7
At1g08320.1 68414.m00920 bZIP family transcription factor contai... 27 8.7
>At1g24460.1 68414.m03081 myosin-related contains TIGRFAM TIGR01612:
reticulocyte binding protein; similar to Myosin heavy
chain, non-muscle (Zipper protein) (Myosin II)
(SP:Q99323) {Drosophila melanogaster} similar to EST
gb|T76116
Length = 1730
Score = 34.3 bits (75), Expect = 0.075
Identities = 22/79 (27%), Positives = 38/79 (48%), Gaps = 3/79 (3%)
Frame = +1
Query: 286 EEASRIRSEQTNNFIVATKEALDAKMETHEEKREAYINELRSRLKDH---LEGVEKTRLT 456
E A+R+ Q + + E + ++E ++ + + + L D LE E T+
Sbjct: 352 ELANRLTELQEKEIALESSEVMKGQLEQSLTEKTDELEKCYAELNDRSVSLEAYELTKKE 411
Query: 457 LEQQTAEVYKAIEDKMTKL 513
LEQ AE K +E+ +TKL
Sbjct: 412 LEQSLAEKTKELEECLTKL 430
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 33.1 bits (72), Expect = 0.17
Identities = 26/82 (31%), Positives = 40/82 (48%), Gaps = 5/82 (6%)
Frame = +1
Query: 175 KDSFRRRDPREAEGSRREET*LGS**EAAIAQKM--AKIEEASRIRSEQTNNFIVATK-- 342
+++ RR E + EE L E Q+ A+ EEA R R E+ ++ K
Sbjct: 417 QEALARRQEAEERKKKEEEEKLRKEEEERRRQEELEAQAEEAKRKRKEKEKEKLLRKKLE 476
Query: 343 -EALDAKMETHEEKREAYINEL 405
+ L AK +T +KREA+ N+L
Sbjct: 477 GKLLTAKQKTEAQKREAFKNQL 498
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 33.1 bits (72), Expect = 0.17
Identities = 26/82 (31%), Positives = 40/82 (48%), Gaps = 5/82 (6%)
Frame = +1
Query: 175 KDSFRRRDPREAEGSRREET*LGS**EAAIAQKM--AKIEEASRIRSEQTNNFIVATK-- 342
+++ RR E + EE L E Q+ A+ EEA R R E+ ++ K
Sbjct: 370 QEALARRQEAEERKKKEEEEKLRKEEEERRRQEELEAQAEEAKRKRKEKEKEKLLRKKLE 429
Query: 343 -EALDAKMETHEEKREAYINEL 405
+ L AK +T +KREA+ N+L
Sbjct: 430 GKLLTAKQKTEAQKREAFKNQL 451
>At4g38070.1 68417.m05377 bHLH family protein contains Pfam profile:
PF00010 helix-loop-helix DNA-binding domain; PMID:
12679534; putative bHLH131 transcription factor
Length = 1513
Score = 32.7 bits (71), Expect = 0.23
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = +3
Query: 534 LKKMIERLREHEEQVRKVRAGNQEKFQQLESSIQEKLQQAAD 659
++K +ER +E E+ + + QEK ++ E+ +EKL Q D
Sbjct: 511 MQKEVERFKEMVEESSRFQTQMQEKMKEAENDYEEKLLQVCD 552
>At1g12080.2 68414.m01397 expressed protein
Length = 138
Score = 32.7 bits (71), Expect = 0.23
Identities = 20/71 (28%), Positives = 38/71 (53%)
Frame = +2
Query: 41 VEAMEVETKSTEIRCQEMSKGGLAYEVILAEPVGVPVPRRADSPEKTPSVEEIQEKLKAA 220
VE E + + TE + +E + EVI PV ++A+ +TP+V E ++K +
Sbjct: 62 VEEAEKKDEETEKKTEEKDE---KTEVITETPVVEEEEKKAEEVTETPAVVEEEKKTEVV 118
Query: 221 EERRRSLEASK 253
EE++ + A++
Sbjct: 119 EEKQTEVAAAE 129
>At5g16730.1 68418.m01959 expressed protein weak similarity to
microtubule binding protein D-CLIP-190 [Drosophila
melanogaster] GI:2773363, SMC2-like condensin
[Arabidopsis thaliana] GI:14279543
Length = 853
Score = 30.3 bits (65), Expect = 1.2
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = +2
Query: 143 VPVPRRADSPEKTPSVEEIQEKLKAAEERRRSLEASK 253
V + +SP+ T + +I+E LK A ER SLE K
Sbjct: 85 VAAVKGTESPQTTTRLSQIKEDLKKANERISSLEKDK 121
>At1g12080.1 68414.m01396 expressed protein
Length = 104
Score = 30.3 bits (65), Expect = 1.2
Identities = 19/68 (27%), Positives = 34/68 (50%)
Frame = +2
Query: 50 MEVETKSTEIRCQEMSKGGLAYEVILAEPVGVPVPRRADSPEKTPSVEEIQEKLKAAEER 229
+E E K E + K EVI PV ++A+ +TP+V E ++K + EE+
Sbjct: 29 VETEKKDEETEKKTEEKDEKT-EVITETPVVEEEEKKAEEVTETPAVVEEEKKTEVVEEK 87
Query: 230 RRSLEASK 253
+ + A++
Sbjct: 88 QTEVAAAE 95
>At2g07190.1 68415.m00824 hypothetical protein
Length = 452
Score = 29.9 bits (64), Expect = 1.6
Identities = 14/47 (29%), Positives = 24/47 (51%)
Frame = +1
Query: 364 ETHEEKREAYINELRSRLKDHLEGVEKTRLTLEQQTAEVYKAIEDKM 504
E +EE + +N + + DH K +L ++ TA + A+ DKM
Sbjct: 267 EGNEENHVSGMNRSKKKQSDHGAETRKKKLLCQRATATTHGALNDKM 313
>At1g67230.1 68414.m07652 expressed protein
Length = 1132
Score = 29.9 bits (64), Expect = 1.6
Identities = 24/82 (29%), Positives = 41/82 (50%), Gaps = 1/82 (1%)
Frame = +1
Query: 259 AIAQKMAKIEEASRIRSEQTNNFIVATKEALDAKMETHEEKREAYINELRSRLKDHLEGV 438
++ K+A++E+ + + ++ALD K+E H+EK N+ RLK + G
Sbjct: 370 SLKSKVAEVEKREA-EWKHMEEKVAKREQALDRKLEKHKEKE----NDFDLRLKG-ISGR 423
Query: 439 EKTRLTLEQQ-TAEVYKAIEDK 501
EK + E+ E K +EDK
Sbjct: 424 EKALKSEEKALETEKKKLLEDK 445
>At2g18540.1 68415.m02160 cupin family protein contains Pfam profile
PF00190: Cupin
Length = 707
Score = 29.5 bits (63), Expect = 2.1
Identities = 30/109 (27%), Positives = 45/109 (41%), Gaps = 1/109 (0%)
Frame = +1
Query: 187 RRRDPREAEGSRREET*LGS**EAAIAQKMAKIEEASRIRSEQTNNFIVATKEALDAKME 366
+RR+ E E R+EE EA K + EEA R E+T +EA + E
Sbjct: 436 KRREEEEIERRRKEEEEARKREEA----KRREEEEAKRREEEETERKKREEEEARKREEE 491
Query: 367 THEEKREA-YINELRSRLKDHLEGVEKTRLTLEQQTAEVYKAIEDKMTK 510
E+ EA E R + ++ E K E++ K E++ K
Sbjct: 492 RKREEEEAKRREEERKKREEEAEQARKREEEREKEEEMAKKREEERQRK 540
>At3g16000.1 68416.m02024 matrix-localized MAR DNA-binding
protein-related similar to matrix-localized MAR DNA
binding protein MFP1 GI:1771158 from [Lycopersicon
esculentum]
Length = 726
Score = 29.1 bits (62), Expect = 2.8
Identities = 24/88 (27%), Positives = 40/88 (45%), Gaps = 9/88 (10%)
Frame = +1
Query: 277 AKIEEASRIRSEQTNNFIVATKEALDAKMETHEEKRE--AYINELRSRLKDHLEGV---- 438
+K+E I E+ + LDA+ + +E A +LR R+KD LEGV
Sbjct: 472 SKLESELAIVHEEWKEAKERYERNLDAEKQKNEISASELALEKDLRRRVKDELEGVTHEL 531
Query: 439 ---EKTRLTLEQQTAEVYKAIEDKMTKL 513
+L+++ E+YK +E +L
Sbjct: 532 KESSVKNQSLQKELVEIYKKVETSNKEL 559
>At1g40133.1 68414.m04768 hypothetical protein
Length = 663
Score = 29.1 bits (62), Expect = 2.8
Identities = 15/48 (31%), Positives = 26/48 (54%)
Frame = +1
Query: 259 AIAQKMAKIEEASRIRSEQTNNFIVATKEALDAKMETHEEKREAYINE 402
A +M +++ + + NN A +A AKME E +REA++N+
Sbjct: 456 ASMNRMVHSYDSAMRNNIEANNRDEALSQAAAAKMEKEEVEREAFVNK 503
>At5g28430.1 68418.m03453 hypothetical protein
Length = 486
Score = 28.7 bits (61), Expect = 3.8
Identities = 29/88 (32%), Positives = 47/88 (53%), Gaps = 10/88 (11%)
Frame = +1
Query: 262 IAQKMAKIEEASRIRS-EQTNNFIVATKEALDAKMETHEEKREAYINE------LRSRLK 420
+A K+A+ E SRI++ E+ N A EA AK+E E +R AY+N+ LK
Sbjct: 271 VAGKLAEAE--SRIQAIEREKN--EALSEAAAAKLEREEVERMAYVNKENAIKMAEQNLK 326
Query: 421 DHLEGVEKTRLTLEQ---QTAEVYKAIE 495
+ E V R+ E+ + +EV +A++
Sbjct: 327 ANSEIVRLKRMLSEERGLRDSEVARAVQ 354
>At3g26420.1 68416.m03295 glycine-rich RNA-binding protein similar
to RNA-binding protein (RZ-1) GB:BAA12064 [Nicotiana
sylvestris]; contains Pfam profile: PF00076 RNA
recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 245
Score = 28.7 bits (61), Expect = 3.8
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +3
Query: 336 HQGGSRRQDGDPRGKTRGLHQRAALPSQGSS*G 434
HQGG+ R + RG+ RG + + PS G G
Sbjct: 86 HQGGAGRDNDGDRGRDRGYDRDRSRPSGGRGGG 118
>At3g05900.1 68416.m00664 neurofilament protein-related similar to
NF-180 (GI:632549) [Petromyzon marinus] similar to
Neurofilament triplet H protein (200 kDa neurofilament
protein) (Neurofilament heavy polypeptide) (NF-H)
(Swiss-Prot:P12036) [Homo sapiens]
Length = 673
Score = 27.9 bits (59), Expect = 6.6
Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = +2
Query: 74 EIRCQEMSKGGLAYEVILAEPVGVPVPRRADSPEKTPSVEE-IQEKLKAAEERR 232
E + ++S+ EV EPV V + A+ E+ P+VE ++E+ K EE +
Sbjct: 147 ETKIVDVSESTDEAEVQQVEPVDVQPVKDAEKAEEKPTVESVVEEETKDREETK 200
>At1g64330.1 68414.m07290 myosin heavy chain-related similar to
myosin heavy chain (GI:1850913) [Entamoeba histolytica];
similar to Intracellular protein transport protein USO1
(Swiss-Prot:P25386) [Saccharomyces cerevisiae]
Length = 555
Score = 27.9 bits (59), Expect = 6.6
Identities = 17/60 (28%), Positives = 31/60 (51%)
Frame = +1
Query: 337 TKEALDAKMETHEEKREAYINELRSRLKDHLEGVEKTRLTLEQQTAEVYKAIEDKMTKLP 516
T+ L+ K+E +++R+ EL S+ KDH +E+ L+ Q E +E + + P
Sbjct: 196 TESDLNQKLEDVKKERDGLEAELASKAKDHESTLEEVN-RLQGQKNETEAELEREKQEKP 254
>At5g61040.1 68418.m07660 expressed protein
Length = 590
Score = 27.5 bits (58), Expect = 8.7
Identities = 17/49 (34%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
Frame = +1
Query: 307 SEQTNNFIVATKEALDA-KMETHEEKREAYINELRSRLKDHLEGVEKTR 450
S ++F V +E LD +E EEK ++NE+ +L + VEKT+
Sbjct: 521 SHHKDDFKVQEQELLDLIGIEDEEEKSSDFVNEMEKQLIKQI--VEKTK 567
>At5g08780.1 68418.m01041 histone H1/H5 family protein contains Pfam
domain, PF00538: linker histone H1 and H5 family
Length = 457
Score = 27.5 bits (58), Expect = 8.7
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +2
Query: 41 VEAMEVETKSTEIRCQEMSKGGLAYEV 121
VE ++VE E R + S+GG YEV
Sbjct: 229 VEVVDVENSENEARIEANSRGGELYEV 255
>At4g17220.1 68417.m02590 expressed protein
Length = 513
Score = 27.5 bits (58), Expect = 8.7
Identities = 18/75 (24%), Positives = 39/75 (52%), Gaps = 2/75 (2%)
Frame = +1
Query: 283 IEEASRIRSE--QTNNFIVATKEALDAKMETHEEKREAYINELRSRLKDHLEGVEKTRLT 456
+ + S ++S+ + ++A K ++A + T+EE ++ ELR ++ E + T
Sbjct: 9 VSDTSSLQSQLKEKEKELLAAKAEVEA-LRTNEELKDRVFKELRENVRKLEEKLGATENQ 67
Query: 457 LEQQTAEVYKAIEDK 501
++Q+ E K E+K
Sbjct: 68 VDQKELERKKLEEEK 82
>At4g02510.1 68417.m00343 chloroplast outer membrane protein,
putative similar to chloroplast protein import component
Toc159 [Pisum sativum] GI:8489806, chloroplast outer
envelope protein 86 [Pisum sativum] GI:599958,
GTP-binding protein [Pisum sativum] GI:576509
Length = 1503
Score = 27.5 bits (58), Expect = 8.7
Identities = 22/81 (27%), Positives = 36/81 (44%), Gaps = 3/81 (3%)
Frame = +1
Query: 262 IAQKMAKIEEASRIRSEQTNNFIVATKEALD-AKMETHEEKREAYINEL--RSRLKDHLE 432
+ + ++ IEE + + NF +A KE + AK+E E K + EL LK
Sbjct: 506 VDKTISNIEEPDDLTAAYDGNFELAVKEISEAAKVEPDEPKVGVEVEELPVSESLKVGSV 565
Query: 433 GVEKTRLTLEQQTAEVYKAIE 495
E+ + + EV K +E
Sbjct: 566 DAEEDSIPAAESQFEVRKVVE 586
>At3g06020.1 68416.m00687 expressed protein ; expression supported
by MPSS
Length = 300
Score = 27.5 bits (58), Expect = 8.7
Identities = 11/42 (26%), Positives = 28/42 (66%)
Frame = +3
Query: 528 ENLKKMIERLREHEEQVRKVRAGNQEKFQQLESSIQEKLQQA 653
E ++ IE +R++EE++ + + +EK ++++ EK+Q++
Sbjct: 235 EEEEETIETVRDNEEEIPEYKEEEEEKEEEIKVKGVEKVQRS 276
>At2g37080.1 68415.m04550 myosin heavy chain-related low similarity
to myosin heavy chain [Rana catesbeiana] GI:4249701
Length = 583
Score = 27.5 bits (58), Expect = 8.7
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +2
Query: 155 RRADSPEKTPSVEEIQEKLKAAEERRRSLEASK 253
R +PE + ++QE+LK A+E+ + EA K
Sbjct: 72 RTGKTPELASQISQLQEELKKAKEQLSASEALK 104
>At2g31130.1 68415.m03801 expressed protein
Length = 419
Score = 27.5 bits (58), Expect = 8.7
Identities = 16/56 (28%), Positives = 31/56 (55%), Gaps = 2/56 (3%)
Frame = +1
Query: 343 EALDAKMETHEEKREAYINELRSRLKDHLEG--VEKTRLTLEQQTAEVYKAIEDKM 504
E+ D + ET+ E + ++E +S + HL G VE++ + +++ E + DKM
Sbjct: 300 ESDDCRKETNVETSSSSVSEPKSEILQHLSGRSVEESCILVDRD--EFHSVFPDKM 353
>At1g08320.1 68414.m00920 bZIP family transcription factor contains
Pfam profile: PF00170 bZIP transcription factor
Length = 481
Score = 27.5 bits (58), Expect = 8.7
Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 6/51 (11%)
Frame = +3
Query: 540 KMIERLREHEEQVRKVRAGNQEKFQQLESS------IQEKLQQAADRRLLI 674
K + RL ++ E RK R + QQLESS ++++LQ+A + L +
Sbjct: 178 KTLRRLAQNREAARKSRLRKKAYVQQLESSRIKLSQLEQELQRARSQGLFM 228
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,408,604
Number of Sequences: 28952
Number of extensions: 196463
Number of successful extensions: 992
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 929
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 991
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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