SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021870X
         (483 letters)

Database: rice 
           37,544 sequences; 14,793,348 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

03_06_0298 - 32925441-32925998,32926371-32926730,32927161-329272...   107   5e-24
07_01_0578 + 4295386-4296489,4297394-4297507                          107   6e-24
10_02_0199 + 6668902-6669293,6669825-6669882                           34   0.069
02_02_0147 - 7176478-7176832,7177006-7177334                           31   0.37 
12_02_1212 - 27043723-27044241,27044483-27044850,27045513-27045753     31   0.48 
11_01_0319 - 2396835-2396951,2397035-2397105,2397219-2397297,239...    31   0.48 
02_04_0607 - 24302582-24303075,24303150-24304197                       31   0.48 
07_03_1678 + 28606656-28606784,28607161-28607352,28607431-28607988     30   0.85 
07_03_0491 - 18707721-18708179                                         30   0.85 
05_03_0163 + 9070781-9071320                                           30   1.1  
03_01_0123 + 965657-966214,967827-967995,968632-969236,969333-96...    30   1.1  
12_01_0073 + 613316-613447,613611-613802,614067-614232,614460-61...    29   1.5  
08_02_1017 - 23616244-23616381,23616471-23616537,23617160-236172...    29   1.5  
05_05_0142 - 22673607-22674134                                         29   2.6  
02_05_0197 - 26641356-26642188,26642312-26642398,26643373-266434...    29   2.6  
05_02_0152 + 7126802-7127315,7127451-7129190,7130718-7131778           28   3.4  
03_01_0520 - 3900387-3900613,3900812-3900853,3902092-3902210,390...    28   3.4  
01_07_0065 - 40851101-40851247,40851352-40851495,40851781-408518...    28   3.4  
02_01_0177 + 1214226-1214294,1214707-1214794,1216095-1216308,121...    28   4.5  
12_02_0634 + 21416205-21416954                                         27   6.0  
10_08_0219 - 15972061-15972110,15972411-15972632,15972673-15972955     27   6.0  
05_03_0235 - 10747649-10748118,10748226-10748314,10748477-107485...    27   6.0  
04_04_1660 - 35140641-35141894                                         27   6.0  
04_03_0126 + 11524567-11524967,11525113-11525647,11525669-11526043     27   6.0  
01_03_0008 + 11594061-11594078,11594201-11594285,11594506-11595149     27   6.0  
11_01_0072 + 570345-570476,570640-570831,571095-571260,571487-57...    27   7.9  
09_06_0153 - 21243108-21245660                                         27   7.9  
05_06_0214 - 26425100-26425922,26426008-26426058,26426183-264262...    27   7.9  

>03_06_0298 -
           32925441-32925998,32926371-32926730,32927161-32927230,
           32927642-32927797,32929181-32929242,32929339-32929352,
           32930421-32930520,32931474-32932574
          Length = 806

 Score =  107 bits (257), Expect = 5e-24
 Identities = 46/53 (86%), Positives = 49/53 (92%)
 Frame = +3

Query: 255 AESWGTGRAVARIPRVRGGGTHRSGHGAFGNMCRGGRIFAPTKRCRRWHRRVN 413
           AESWGTGRAV+RIPRV GGGTHR+G GAFGNMCRGGR+FAPTK  RRWHRRVN
Sbjct: 67  AESWGTGRAVSRIPRVPGGGTHRAGQGAFGNMCRGGRMFAPTKIWRRWHRRVN 119



 Score = 61.7 bits (143), Expect = 3e-10
 Identities = 32/63 (50%), Positives = 39/63 (61%), Gaps = 1/63 (1%)
 Frame = +1

Query: 67  SVARPLVSVYS-EKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAG 243
           + ARPLVSV + E       A   +P V +APIRPD+V   H  +S N RQPY VS+ AG
Sbjct: 3   AAARPLVSVKALEGDMATDSAGIQMPQVLRAPIRPDVVTFTHKLLSCNRRQPYAVSRRAG 62

Query: 244 HQT 252
           HQT
Sbjct: 63  HQT 65


>07_01_0578 + 4295386-4296489,4297394-4297507
          Length = 405

 Score =  107 bits (256), Expect = 6e-24
 Identities = 45/53 (84%), Positives = 49/53 (92%)
 Frame = +3

Query: 255 AESWGTGRAVARIPRVRGGGTHRSGHGAFGNMCRGGRIFAPTKRCRRWHRRVN 413
           AESWGTGRAV+RIPRV GGGTHR+G GAFGNMCRGGR+FAPTK  R+WHRRVN
Sbjct: 68  AESWGTGRAVSRIPRVPGGGTHRAGQGAFGNMCRGGRMFAPTKTWRKWHRRVN 120



 Score = 59.7 bits (138), Expect = 1e-09
 Identities = 31/61 (50%), Positives = 41/61 (67%), Gaps = 2/61 (3%)
 Frame = +1

Query: 76  RPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHQ 249
           RPLVSV + + +  T   ++  L  VF+AP+RPD+V  VH  +S N RQPY VS+ AGHQ
Sbjct: 6   RPLVSVKALEGDMATDNSSSLALAEVFRAPLRPDVVRFVHRLLSCNKRQPYAVSRRAGHQ 65

Query: 250 T 252
           T
Sbjct: 66  T 66


>10_02_0199 + 6668902-6669293,6669825-6669882
          Length = 149

 Score = 33.9 bits (74), Expect = 0.069
 Identities = 21/47 (44%), Positives = 23/47 (48%)
 Frame = +3

Query: 225 REQGGWSPNQAESWGTGRAVARIPRVRGGGTHRSGHGAFGNMCRGGR 365
           RE GG     A+SW TGRA A + R R GG  R    A  N   G R
Sbjct: 30  REGGGG----AQSWATGRAAAELGRGRKGGRRRGLAQAAANSGEGNR 72


>02_02_0147 - 7176478-7176832,7177006-7177334
          Length = 227

 Score = 31.5 bits (68), Expect = 0.37
 Identities = 21/62 (33%), Positives = 27/62 (43%), Gaps = 1/62 (1%)
 Frame = +3

Query: 225 REQGGWSPNQAESWGTGRAV-ARIPRVRGGGTHRSGHGAFGNMCRGGRIFAPTKRCRRWH 401
           R+ GGWS   A + G G  V +R P + G        G  G   R    + P +R RR  
Sbjct: 88  RQHGGWSTETAAAGGCGNGVHSRRPHLAGVVAIGGVGGQLGAERRSRWQWRPVRRKRRGR 147

Query: 402 RR 407
           RR
Sbjct: 148 RR 149


>12_02_1212 - 27043723-27044241,27044483-27044850,27045513-27045753
          Length = 375

 Score = 31.1 bits (67), Expect = 0.48
 Identities = 15/47 (31%), Positives = 23/47 (48%), Gaps = 3/47 (6%)
 Frame = -1

Query: 399 ANAGSASWGRRYVHHDTCYRRHRDRTYEYH---HHGHAEFGRQHVQY 268
           A A +AS  +R  H       H+ + + YH   H GH    R+H+Q+
Sbjct: 223 AAAAAASMNKRISHELQHIHHHQQQQFYYHEVVHDGHGHHHRRHLQH 269


>11_01_0319 -
           2396835-2396951,2397035-2397105,2397219-2397297,
           2397417-2397458,2397670-2397723,2397836-2397868,
           2397959-2398039,2398144-2398230,2398313-2398388,
           2398633-2398808,2398912-2399034,2399249-2399361,
           2399521-2399632,2400054-2400273,2401986-2402314,
           2402408-2402713,2403580-2403878,2404079-2404181,
           2404266-2404369,2404840-2404906,2404911-2405076,
           2405241-2405956
          Length = 1157

 Score = 31.1 bits (67), Expect = 0.48
 Identities = 21/90 (23%), Positives = 40/90 (44%), Gaps = 3/90 (3%)
 Frame = -1

Query: 399 ANAGSASWGRRYVHHDTCYRRHRDRTYEYHHHGHAEFGR---QHVQYPMIQPGLVTSLLA 229
           +++G     RR+ H ++  RR RD+++     GH E  R   Q  + P   P L+  ++ 
Sbjct: 44  SSSGDDDRSRRHRHDESSRRRQRDQSHRRDRGGHDERRRRSPQRRKEPTPPPPLLPEMIP 103

Query: 228 HAVGLPRVLGHRNVNIIDQVRTDGRLEHER 139
              G   +   R   ++ +V      E++R
Sbjct: 104 GRTGGIYIPPFRMAQMMREVEDKSSAEYQR 133


>02_04_0607 - 24302582-24303075,24303150-24304197
          Length = 513

 Score = 31.1 bits (67), Expect = 0.48
 Identities = 17/57 (29%), Positives = 25/57 (43%), Gaps = 2/57 (3%)
 Frame = -1

Query: 441 PQRPLSAVGG*RDGANAGSASWGRRYVHHDTCYRRH--RDRTYEYHHHGHAEFGRQH 277
           P+ P S  GG   G  A S   G+  + ++    R   R +   +HHHGH +    H
Sbjct: 38  PELPESVAGGGGGGDPAVSPGLGKLNIRYNATSGRAGLRFQQLHHHHHGHVDVRHHH 94


>07_03_1678 + 28606656-28606784,28607161-28607352,28607431-28607988
          Length = 292

 Score = 30.3 bits (65), Expect = 0.85
 Identities = 19/52 (36%), Positives = 22/52 (42%)
 Frame = +3

Query: 39  ASLK*RNESIGSPTFSVGVFREE*DGAGCSQAPPVRVQGAHPSGPGQ*CSRF 194
           ASL   N  +  P+F V          G SQA  V + GAH  G  Q C  F
Sbjct: 125 ASLALANSDLPPPSFDVANLTASFAAKGLSQADMVALSGAHTVGQAQ-CQNF 175


>07_03_0491 - 18707721-18708179
          Length = 152

 Score = 30.3 bits (65), Expect = 0.85
 Identities = 20/63 (31%), Positives = 25/63 (39%)
 Frame = -1

Query: 465 GTPVAATVPQRPLSAVGG*RDGANAGSASWGRRYVHHDTCYRRHRDRTYEYHHHGHAEFG 286
           GT  A +   RP SAVGG       G+   G   V  +    RHR+R +      HA   
Sbjct: 71  GTAAARSSRPRPGSAVGGRSTAPEPGAGGDGGGAVSGEGGVSRHRERRWRRRTPPHAVIA 130

Query: 285 RQH 277
             H
Sbjct: 131 DTH 133


>05_03_0163 + 9070781-9071320
          Length = 179

 Score = 29.9 bits (64), Expect = 1.1
 Identities = 16/37 (43%), Positives = 19/37 (51%)
 Frame = +3

Query: 225 REQGGWSPNQAESWGTGRAVARIPRVRGGGTHRSGHG 335
           R  GGWS  +AE WG G   ++     GGG  RS  G
Sbjct: 15  RGAGGWS-QRAEGWGGGGGRSQRAEGGGGGGGRSRRG 50


>03_01_0123 +
           965657-966214,967827-967995,968632-969236,969333-969434
          Length = 477

 Score = 29.9 bits (64), Expect = 1.1
 Identities = 12/25 (48%), Positives = 14/25 (56%)
 Frame = +3

Query: 210 EAALLREQGGWSPNQAESWGTGRAV 284
           EAALLR  G W+    + WG G  V
Sbjct: 367 EAALLRAAGDWARGAGQRWGVGDVV 391


>12_01_0073 +
           613316-613447,613611-613802,614067-614232,614460-614845
          Length = 291

 Score = 29.5 bits (63), Expect = 1.5
 Identities = 17/52 (32%), Positives = 23/52 (44%)
 Frame = +3

Query: 39  ASLK*RNESIGSPTFSVGVFREE*DGAGCSQAPPVRVQGAHPSGPGQ*CSRF 194
           ASL   N  I +PTF +G   +     G S    + + GAH  G  + C  F
Sbjct: 126 ASLDTANNDIPAPTFDLGDLTKSFSNKGLSATDMIALSGAHTIGQAR-CVNF 176


>08_02_1017 -
           23616244-23616381,23616471-23616537,23617160-23617238,
           23617330-23617486,23617581-23617719,23617820-23617872,
           23618037-23618120,23618291-23618359,23618488-23618553,
           23618657-23618800,23619331-23619414,23619498-23619596,
           23620097-23620219,23620745-23621290
          Length = 615

 Score = 29.5 bits (63), Expect = 1.5
 Identities = 16/40 (40%), Positives = 21/40 (52%), Gaps = 2/40 (5%)
 Frame = +1

Query: 163 RPDLVNDVHVSMSKNSRQP--YCVSKEAGHQTRLNHGVLD 276
           RP  + DV  S S +S  P     SK++GH   L HG +D
Sbjct: 38  RPSSITDVSRSCSSSSSSPPRSLSSKQSGHADVLAHGAVD 77


>05_05_0142 - 22673607-22674134
          Length = 175

 Score = 28.7 bits (61), Expect = 2.6
 Identities = 19/56 (33%), Positives = 23/56 (41%)
 Frame = +3

Query: 240 WSPNQAESWGTGRAVARIPRVRGGGTHRSGHGAFGNMCRGGRIFAPTKRCRRWHRR 407
           W P    SW  G A AR+  +  GG  R   G      RG   +   +R RRW  R
Sbjct: 68  WIPTS--SWEVGDA-ARMTALMVGGAGRRREGRGRGRWRGRAAYTRGRRGRRWRGR 120


>02_05_0197 - 26641356-26642188,26642312-26642398,26643373-26643452,
            26643948-26644120,26644227-26644308,26644623-26644758,
            26644777-26644833,26644905-26645120,26645939-26646335,
            26646458-26646544,26647063-26647324,26648892-26648972,
            26649176-26650350
          Length = 1221

 Score = 28.7 bits (61), Expect = 2.6
 Identities = 14/41 (34%), Positives = 20/41 (48%), Gaps = 2/41 (4%)
 Frame = +3

Query: 225  REQGGWSPNQAESWGTGRAVARIPRVRGGGT--HRSGHGAF 341
            R + GW  +QA+     + + R P  +GGG    R G G F
Sbjct: 1121 RREQGWDAHQAQVGAQHQNLPRFPPQQGGGNIGERQGQGQF 1161


>05_02_0152 + 7126802-7127315,7127451-7129190,7130718-7131778
          Length = 1104

 Score = 28.3 bits (60), Expect = 3.4
 Identities = 21/65 (32%), Positives = 26/65 (40%), Gaps = 3/65 (4%)
 Frame = +3

Query: 162  PSGPGQ*CSRFYVQELEAALLREQGGWSPNQ---AESWGTGRAVARIPRVRGGGTHRSGH 332
            P G G    R  V+  +AA     GGW   +   A SW T      +   R GG  +  H
Sbjct: 846  PRGDGGKRRRTVVERWQAA-----GGWRDGRERPAGSWATAATATLLGPTRYGGVAQETH 900

Query: 333  GAFGN 347
            G  GN
Sbjct: 901  GDDGN 905


>03_01_0520 -
           3900387-3900613,3900812-3900853,3902092-3902210,
           3903633-3903712,3903829-3903856,3904151-3904272,
           3904714-3904857,3904897-3906327
          Length = 730

 Score = 28.3 bits (60), Expect = 3.4
 Identities = 13/45 (28%), Positives = 20/45 (44%)
 Frame = -1

Query: 390 GSASWGRRYVHHDTCYRRHRDRTYEYHHHGHAEFGRQHVQYPMIQ 256
           G  S+  +  HH    + H  + YE+ +  H +  RQ  QY   Q
Sbjct: 140 GEDSFPLQQNHHQQYQQHHHQQQYEHQNRIHFQHHRQQQQYQQHQ 184


>01_07_0065 -
           40851101-40851247,40851352-40851495,40851781-40851872,
           40851954-40852035,40852127-40852193,40852284-40852375,
           40852470-40852586,40852764-40852881,40852969-40853161,
           40853258-40853339,40853607-40853744,40853829-40854023
          Length = 488

 Score = 28.3 bits (60), Expect = 3.4
 Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
 Frame = -1

Query: 375 GRRYVHHDTCYRRHRDRTYEYHHHGHAEFGRQHVQYPMIQP-GLVTSLLA 229
           G  +++H   +RRH+D T +  H G  +       Y   +P GL++SL A
Sbjct: 267 GINHMYHRPAWRRHKDCTDDSPHEGPFKTDSPAWCYAPFEPEGLLSSLSA 316


>02_01_0177 + 1214226-1214294,1214707-1214794,1216095-1216308,
            1216413-1216738,1216860-1217013,1217377-1217438,
            1218078-1218133,1219456-1221279,1221775-1222698,
            1222855-1222866
          Length = 1242

 Score = 27.9 bits (59), Expect = 4.5
 Identities = 16/47 (34%), Positives = 19/47 (40%)
 Frame = +3

Query: 225  REQGGWSPNQAESWGTGRAVARIPRVRGGGTHRSGHGAFGNMCRGGR 365
            R+ GG S       G+GR      R RG    R G+G       GGR
Sbjct: 1176 RQGGGRSGYSGRGGGSGRGGGGYQRDRGSQGSRGGYGGGSRFQDGGR 1222


>12_02_0634 + 21416205-21416954
          Length = 249

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 30/85 (35%), Positives = 36/85 (42%), Gaps = 10/85 (11%)
 Frame = +3

Query: 162 PSGPGQ*CSRFYVQELEAALLREQGGWS---PN-QAESWGTGRAVARIP----RVRGGGT 317
           P  P +  +R  V+   A + R   G     P  +A SW  GR   R P    R RGG  
Sbjct: 66  PGQPEEMSTRRKVRPARATVFRRCSGEEEVIPGMRAASWSRGRWWRRRPALRGRGRGGRR 125

Query: 318 HRSGHGAFGNMCRGGRIFA--PTKR 386
            RSG  A G   R GR+    P KR
Sbjct: 126 RRSGGDATGR--RRGRVSGAFPAKR 148


>10_08_0219 - 15972061-15972110,15972411-15972632,15972673-15972955
          Length = 184

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 13/37 (35%), Positives = 17/37 (45%)
 Frame = +3

Query: 234 GGWSPNQAESWGTGRAVARIPRVRGGGTHRSGHGAFG 344
           GG   N    WG+G   +   +  GGG + SG G  G
Sbjct: 38  GGGGSNGGSGWGSGSG-SGYGQASGGGAYASGGGGAG 73


>05_03_0235 -
           10747649-10748118,10748226-10748314,10748477-10748574,
           10748934-10749046,10749107-10749200,10749557-10749589,
           10749734-10749851,10750110-10750210,10751036-10751233,
           10751337-10751471,10751752-10751830,10753650-10753738,
           10753835-10753987,10754100-10754285
          Length = 651

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 13/36 (36%), Positives = 17/36 (47%), Gaps = 3/36 (8%)
 Frame = -1

Query: 375 GRRYVHHDTCYRRHRDRTYEYHHH---GHAEFGRQH 277
           GR + HH     RH     ++HHH   GH +  R H
Sbjct: 533 GRHHRHHHDHRHRHGHSHGDHHHHYHGGHHQRRRHH 568


>04_04_1660 - 35140641-35141894
          Length = 417

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 10/30 (33%), Positives = 16/30 (53%)
 Frame = +3

Query: 234 GGWSPNQAESWGTGRAVARIPRVRGGGTHR 323
           GGW+P +  +WG    + + PR+  G   R
Sbjct: 255 GGWAPVERSAWGDVFEILKRPRLLAGAGGR 284


>04_03_0126 + 11524567-11524967,11525113-11525647,11525669-11526043
          Length = 436

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 11/40 (27%), Positives = 20/40 (50%)
 Frame = -1

Query: 285 RQHVQYPMIQPGLVTSLLAHAVGLPRVLGHRNVNIIDQVR 166
           ++HV +P    G +   L+ A  L R+L H  + ++   R
Sbjct: 5   KKHVLFPFTSKGHIAGFLSLASRLHRILPHATITLVSTPR 44


>01_03_0008 + 11594061-11594078,11594201-11594285,11594506-11595149
          Length = 248

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 10/17 (58%), Positives = 13/17 (76%), Gaps = 1/17 (5%)
 Frame = -1

Query: 324 TYEYHH-HGHAEFGRQH 277
           T+++HH HGH  FGR H
Sbjct: 144 THDHHHGHGHHLFGRDH 160


>11_01_0072 +
           570345-570476,570640-570831,571095-571260,571487-571872
          Length = 291

 Score = 27.1 bits (57), Expect = 7.9
 Identities = 16/52 (30%), Positives = 22/52 (42%)
 Frame = +3

Query: 39  ASLK*RNESIGSPTFSVGVFREE*DGAGCSQAPPVRVQGAHPSGPGQ*CSRF 194
           ASL   N  I +PT  +G   +     G S    + + GAH  G  + C  F
Sbjct: 126 ASLDTANNDIPAPTLDLGDLTKSFSNKGLSATDMIALSGAHTIGQAR-CVNF 176


>09_06_0153 - 21243108-21245660
          Length = 850

 Score = 27.1 bits (57), Expect = 7.9
 Identities = 14/41 (34%), Positives = 23/41 (56%)
 Frame = -2

Query: 125 APCTVSLFSEYTDTKGRATDRLISLL*RSYTSEIYTNSGDK 3
           APCTV +F++    +G A+ RL + +  S  +    N GD+
Sbjct: 612 APCTVGVFADRPFWRGGASFRLPTKISTSEETTAARNQGDQ 652


>05_06_0214 -
           26425100-26425922,26426008-26426058,26426183-26426256,
           26426344-26426441,26426547-26426671,26426724-26426957,
           26426992-26427037,26427101-26427152,26427331-26427367,
           26427521-26427637,26428287-26428352,26428439-26428518,
           26429102-26429311,26429650-26429739,26430093-26430182,
           26430280-26430411,26430876-26431094,26431203-26431286,
           26431543-26431656,26431857-26431914,26432535-26432602,
           26432847-26432962,26433495-26433574,26433839-26433912
          Length = 1045

 Score = 27.1 bits (57), Expect = 7.9
 Identities = 10/26 (38%), Positives = 16/26 (61%), Gaps = 1/26 (3%)
 Frame = -3

Query: 130 WLHPAPSHSSLN-TPTLKVGLPIDSF 56
           WL P P+H ++N    L + +P+D F
Sbjct: 871 WLKPTPAHVAMNPAEALAMQIPVDHF 896


  Database: rice
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 14,793,348
  Number of sequences in database:  37,544
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,454,319
Number of Sequences: 37544
Number of extensions: 317460
Number of successful extensions: 1405
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 1303
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1393
length of database: 14,793,348
effective HSP length: 77
effective length of database: 11,902,460
effective search space used: 987904180
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -