Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021866X
(524 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch... 47 1e-06
SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 40 2e-04
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 40 2e-04
SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pomb... 25 0.88
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 27 1.7
SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon... 27 2.3
SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces po... 26 4.0
SPBC1289.16c ||SPBC8E4.06|copper amine oxidase |Schizosaccharomy... 26 4.0
SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|c... 26 4.0
SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein ... 25 5.2
SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces... 25 5.2
SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ... 25 5.2
SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schiz... 25 5.2
SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce... 25 6.9
SPAC16A10.03c |||zinc finger protein Pep5/Vps11 |Schizosaccharom... 25 6.9
SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 9.1
>SPBC12C2.05c |||diacylglycerol binding protein Bzz1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 47.2 bits (107), Expect = 1e-06
Identities = 18/50 (36%), Positives = 24/50 (48%)
Frame = +1
Query: 256 TFCSHCKDFIWGFGKQGFQCQVCSVVVHKRCHEYVTFTCPGADKGADSDD 405
T CS+C++ IWG K+G C+ C H RC V C + DD
Sbjct: 409 TSCSYCREIIWGLSKRGCVCKNCGFKCHARCELLVPANCKNGEPEVADDD 458
Score = 29.9 bits (64), Expect = 0.24
Identities = 10/33 (30%), Positives = 20/33 (60%)
Frame = +1
Query: 418 HNFRVYTYSSPTFCDHCGSLLYGLIHQGLKFKH 516
H+F+ ++ PT C +C +++GL +G K+
Sbjct: 398 HDFKHVSFKLPTSCSYCREIIWGLSKRGCVCKN 430
>SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 988
Score = 40.3 bits (90), Expect = 2e-04
Identities = 21/63 (33%), Positives = 26/63 (41%), Gaps = 4/63 (6%)
Frame = +1
Query: 304 GFQCQVCSVVVHKRCHEYVTFTCPGADKGADSD----DTRTRHNFRVYTYSSPTFCDHCG 471
G QC+ CS V HK+C V +C SD R H F + +C HCG
Sbjct: 439 GLQCENCSFVCHKKCVTKVLASCIAQSNSEKSDFGGLRYRIPHRFEPFNSLGAQWCAHCG 498
Query: 472 SLL 480
L
Sbjct: 499 FFL 501
Score = 30.3 bits (65), Expect = 0.18
Identities = 12/40 (30%), Positives = 19/40 (47%)
Frame = +1
Query: 253 ATFCSHCKDFIWGFGKQGFQCQVCSVVVHKRCHEYVTFTC 372
A +C+HC F+ K F+C C + H +C + C
Sbjct: 491 AQWCAHCGFFLPLRRKDCFKCVECGITCHGQCAHLIPDYC 530
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 39.9 bits (89), Expect = 2e-04
Identities = 22/78 (28%), Positives = 33/78 (42%), Gaps = 5/78 (6%)
Frame = +1
Query: 262 CSHCKDFIWGFGKQGFQCQVCSVVVHKRCHEYVTFTCPGADKGADSDD-----TRTRHNF 426
C+ C +F+ G QC C HK+C+ V C + S + R H+F
Sbjct: 419 CALCGEFLKNAA--GMQCIDCHYTCHKKCYPKVVTKCISKSSDSASSEYEKINHRIPHHF 476
Query: 427 RVYTYSSPTFCDHCGSLL 480
+T +C HCG +L
Sbjct: 477 ESHTNIGANWCCHCGYIL 494
>SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 420
Score = 24.6 bits (51), Expect(2) = 0.88
Identities = 10/35 (28%), Positives = 15/35 (42%)
Frame = -1
Query: 224 DLSPGKHSSSLGHPCGLANETPGPLSGHHHRHHNY 120
+L S S H ++ GHHH HH++
Sbjct: 272 NLKKSSESGSTNHHNNHESDKDLHHEGHHHHHHHH 306
Score = 21.8 bits (44), Expect(2) = 0.88
Identities = 6/12 (50%), Positives = 8/12 (66%)
Frame = -1
Query: 143 HHHRHHNYFPRP 108
HHH HH++ P
Sbjct: 305 HHHHHHSHDDDP 316
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 27.1 bits (57), Expect = 1.7
Identities = 11/39 (28%), Positives = 17/39 (43%)
Frame = +1
Query: 232 HAEILQAATFCSHCKDFIWGFGKQGFQCQVCSVVVHKRC 348
H + + C C + +WG +C CS+ H RC
Sbjct: 701 HVNAIFKPSRCYICSESVWG---SELRCFHCSISCHSRC 736
>SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 678
Score = 26.6 bits (56), Expect = 2.3
Identities = 10/27 (37%), Positives = 19/27 (70%)
Frame = -3
Query: 423 VVSRACVVGIRPLVGAGTREGNILVAS 343
V++R+C++ R L+GA T+ G+ V +
Sbjct: 320 VLARSCIIKARTLIGAYTKVGDASVVA 346
>SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 507
Score = 25.8 bits (54), Expect = 4.0
Identities = 19/76 (25%), Positives = 30/76 (39%)
Frame = +2
Query: 266 PTAKISYGALENKDSSAKCAVSWCTSDATSMLPSRVPAPTRGRIPTTHARDTTFAFTRIL 445
PT + A SA +S ATS + + +P+ P A ++T A T
Sbjct: 186 PTTVAASVASSVDTDSASATISSVVDSATSSVSTVIPSSIISAAPPDSASESTPASTSYA 245
Query: 446 LQRSAITVALCCTGSS 493
++ T +GSS
Sbjct: 246 SSTTSATSTSTTSGSS 261
>SPBC1289.16c ||SPBC8E4.06|copper amine oxidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 794
Score = 25.8 bits (54), Expect = 4.0
Identities = 7/14 (50%), Positives = 10/14 (71%)
Frame = -1
Query: 158 GPLSGHHHRHHNYF 117
G GHHH HH+++
Sbjct: 737 GSNGGHHHHHHHHY 750
>SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 386
Score = 25.8 bits (54), Expect = 4.0
Identities = 21/59 (35%), Positives = 26/59 (44%), Gaps = 4/59 (6%)
Frame = -1
Query: 263 QNVAA*RISA*ICDLSPGKHSSSLGHPCGLANET-PGPLSGHHHRH---HNYFPRPPYR 99
QN +S I D P S +P L+ +T P GHHH+H H YF P R
Sbjct: 14 QNPINNNVSVTISDQLP---KPSANNPNLLSVDTRPTHRKGHHHKHSLSHQYFLPPKNR 69
>SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein
Lsb1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 296
Score = 25.4 bits (53), Expect = 5.2
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +3
Query: 438 VFFSNVLRSLWLSAVRAHPPGPEIQ 512
+F SN +R L SAV+A PP P Q
Sbjct: 188 IFPSNYVRLLEDSAVKAQPPPPPPQ 212
>SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 404
Score = 25.4 bits (53), Expect = 5.2
Identities = 10/33 (30%), Positives = 15/33 (45%)
Frame = +1
Query: 259 FCSHCKDFIWGFGKQGFQCQVCSVVVHKRCHEY 357
+C +C G + CQ+C HK C E+
Sbjct: 240 YCGYCSKPSMG-ACWVYGCQLCDTFYHKNCKEH 271
>SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2111
Score = 25.4 bits (53), Expect = 5.2
Identities = 16/51 (31%), Positives = 19/51 (37%)
Frame = +1
Query: 304 GFQCQVCSVVVHKRCHEYVTFTCPGADKGADSDDTRTRHNFRVYTYSSPTF 456
GF C + V HK + C +GA DTR V T TF
Sbjct: 72 GFTCHIKGQVSHKIVTDARLLLCNMTHRGATGADTRDGDGAGVMTGMPYTF 122
>SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 665
Score = 25.4 bits (53), Expect = 5.2
Identities = 10/23 (43%), Positives = 17/23 (73%)
Frame = -1
Query: 413 VRVSSESAPLSAPGHVKVTYSWH 345
+ + SESAP+S P H+ +YS++
Sbjct: 155 LHLRSESAPISLPIHLYKSYSYN 177
>SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1008
Score = 25.0 bits (52), Expect = 6.9
Identities = 10/26 (38%), Positives = 13/26 (50%)
Frame = -1
Query: 149 SGHHHRHHNYFPRPPYRFAVAKCAGK 72
S HHH HN F + ++ K GK
Sbjct: 4 SEHHHHGHNGFSKFLHKLGFGKSKGK 29
>SPAC16A10.03c |||zinc finger protein Pep5/Vps11
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 860
Score = 25.0 bits (52), Expect = 6.9
Identities = 13/49 (26%), Positives = 22/49 (44%)
Frame = +1
Query: 310 QCQVCSVVVHKRCHEYVTFTCPGADKGADSDDTRTRHNFRVYTYSSPTF 456
Q +C VV+ K+ Y+ F K +S+ + R+Y +P F
Sbjct: 93 QSALCCVVLDKQNFVYLQFWSLNPSKKTNSNSPLCLYEHRLYGIPNPPF 141
>SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 425
Score = 24.6 bits (51), Expect = 9.1
Identities = 17/61 (27%), Positives = 29/61 (47%), Gaps = 4/61 (6%)
Frame = +1
Query: 205 CLPGERSQIHAEILQAATF-CSHCKDFIWGFGKQGFQCQVCSVVVHKRCH-EYV--TFTC 372
CLP H I ++ C C ++++ ++ CS +H+RCH EY+ + C
Sbjct: 251 CLPISVFNTHRCIERSTDCNCPICGEYMFN-SRERVIFLSCSHPLHQRCHEEYIRTNYRC 309
Query: 373 P 375
P
Sbjct: 310 P 310
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,305,362
Number of Sequences: 5004
Number of extensions: 48267
Number of successful extensions: 195
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 174
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 214353836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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