Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021857
(831 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 135 8e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 83 4e-17
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 82 8e-17
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 73 4e-14
SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces... 29 1.1
SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 29 1.1
SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 28 1.4
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.5
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.3
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.3
SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos... 26 5.7
SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 5.7
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 10.0
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 10.0
SPAC32A11.03c |phx1||homeobox transcription factor Phx1|Schizosa... 25 10.0
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 135 bits (326), Expect = 8e-33
Identities = 74/170 (43%), Positives = 93/170 (54%), Gaps = 1/170 (0%)
Frame = +3
Query: 255 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESES 434
DLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+E+
Sbjct: 67 DLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEA 126
Query: 435 CDCLQGFQXXXXXXXXXXXXXXXXXXQRSVKSTPTES*THTQ*SPRPK-YQTLSSNHTMR 611
CD LQGFQ + + P +P PK T+ +
Sbjct: 127 CDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNAT 186
Query: 612 LSQFTS*LKTQTKPTASTTRLYTTSASALSNCPHPTYGDLNHLVSLTMSG 761
LS + L + A+ L P+Y DLNHLVS M+G
Sbjct: 187 LSMHQLVENSDETFCIDNEALSSIFANTL-KIKSPSYDDLNHLVSAVMAG 235
Score = 118 bits (283), Expect = 1e-27
Identities = 49/70 (70%), Positives = 58/70 (82%)
Frame = +1
Query: 58 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 237
MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 238 PRAILVTWSP 267
PRA+LV P
Sbjct: 61 PRAVLVDLEP 70
Score = 113 bits (271), Expect = 4e-26
Identities = 51/69 (73%), Positives = 60/69 (86%)
Frame = +2
Query: 509 ISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDIC 688
+SKIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+CIDNEAL I
Sbjct: 152 LSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIF 211
Query: 689 FRTLKLSTP 715
TLK+ +P
Sbjct: 212 ANTLKIKSP 220
Score = 42.7 bits (96), Expect = 6e-05
Identities = 19/24 (79%), Positives = 20/24 (83%)
Frame = +1
Query: 760 VHTCLRFPGQLNGDLRKLAVNMVP 831
V T RFPG+LN DLRKLAVNMVP
Sbjct: 236 VTTSFRFPGELNSDLRKLAVNMVP 259
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 83.4 bits (197), Expect = 4e-17
Identities = 33/67 (49%), Positives = 49/67 (73%)
Frame = +3
Query: 255 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESES 434
DLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+ +++
Sbjct: 73 DLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADN 132
Query: 435 CDCLQGF 455
C LQGF
Sbjct: 133 CSGLQGF 139
Score = 71.3 bits (167), Expect = 2e-13
Identities = 30/69 (43%), Positives = 46/69 (66%)
Frame = +2
Query: 509 ISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDIC 688
+ ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC
Sbjct: 158 LERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDIC 217
Query: 689 FRTLKLSTP 715
R L + P
Sbjct: 218 RRNLDIERP 226
Score = 53.2 bits (122), Expect = 4e-08
Identities = 27/76 (35%), Positives = 39/76 (51%), Gaps = 6/76 (7%)
Frame = +1
Query: 58 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 219
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 220 SGGKYVPRAILVTWSP 267
GK+VPR+I V P
Sbjct: 61 GQGKFVPRSIYVDLEP 76
Score = 26.6 bits (56), Expect = 4.3
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +1
Query: 772 LRFPGQLNGDLRKLAVNMVP 831
LRF G LN DL + N+VP
Sbjct: 246 LRFAGSLNVDLNEFQTNLVP 265
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 82.2 bits (194), Expect = 8e-17
Identities = 33/67 (49%), Positives = 47/67 (70%)
Frame = +3
Query: 255 DLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESES 434
DLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ +++
Sbjct: 69 DLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADN 128
Query: 435 CDCLQGF 455
C LQGF
Sbjct: 129 CSGLQGF 135
Score = 66.9 bits (156), Expect = 3e-12
Identities = 29/74 (39%), Positives = 46/74 (62%)
Frame = +2
Query: 494 YGHPPISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEA 673
+G + ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++ D T+ +DNE+
Sbjct: 149 FGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNES 208
Query: 674 LYDICFRTLKLSTP 715
YDIC R L + P
Sbjct: 209 CYDICRRNLDIERP 222
Score = 59.3 bits (137), Expect = 7e-10
Identities = 29/72 (40%), Positives = 40/72 (55%), Gaps = 2/72 (2%)
Frame = +1
Query: 58 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 231
MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 232 YVPRAILVTWSP 267
YVPR+I V P
Sbjct: 61 YVPRSIYVDLEP 72
Score = 26.2 bits (55), Expect = 5.7
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +1
Query: 772 LRFPGQLNGDLRKLAVNMVP 831
LRF G LN DL + N+VP
Sbjct: 242 LRFEGSLNVDLAEFQTNLVP 261
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 73.3 bits (172), Expect = 4e-14
Identities = 31/69 (44%), Positives = 47/69 (68%)
Frame = +1
Query: 61 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 240
REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 241 RAILVTWSP 267
RAIL+ P
Sbjct: 63 RAILIDLEP 71
Score = 58.8 bits (136), Expect = 9e-10
Identities = 23/69 (33%), Positives = 45/69 (65%), Gaps = 2/69 (2%)
Frame = +3
Query: 255 DLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKES 428
DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +E+
Sbjct: 68 DLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREA 126
Query: 429 ESCDCLQGF 455
+ D L+GF
Sbjct: 127 DGSDSLEGF 135
Score = 57.6 bits (133), Expect = 2e-09
Identities = 28/70 (40%), Positives = 40/70 (57%), Gaps = 1/70 (1%)
Frame = +2
Query: 509 ISKIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDI 685
+ ++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN AL I
Sbjct: 154 LERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHI 213
Query: 686 CFRTLKLSTP 715
L P
Sbjct: 214 AADRLHTQNP 223
Score = 27.1 bits (57), Expect = 3.3
Identities = 9/22 (40%), Positives = 15/22 (68%)
Frame = +1
Query: 766 TCLRFPGQLNGDLRKLAVNMVP 831
T LR+PG +N DL + +++P
Sbjct: 241 TTLRYPGYMNNDLVSIIASLIP 262
>SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 967
Score = 28.7 bits (61), Expect = 1.1
Identities = 17/43 (39%), Positives = 22/43 (51%)
Frame = +3
Query: 225 RQVRAPRHPRDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGN 353
R+V AP+H R GT RS +FR ++ FGQ GN
Sbjct: 486 REVFAPKHARR-RLGTRFHSRSSHRPSVFRDNSVAFGQLDNGN 527
>SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 28.7 bits (61), Expect = 1.1
Identities = 24/69 (34%), Positives = 37/69 (53%), Gaps = 11/69 (15%)
Frame = +2
Query: 491 RYGHPPISKIREEYP-DRIMNTYSVVP--SPKVSDTVVE---PYN--ATLSVHQL---VE 637
+Y H SK++ EYP D+++ SV+ PK VVE P N A L+ H + ++
Sbjct: 392 QYAHVVASKLQREYPRDKVLYYSSVLTEFDPKGIQEVVESLRPNNFFAILAAHSIEKGLD 451
Query: 638 NTDETYCID 664
N ++ Y ID
Sbjct: 452 NKEKFYGID 460
>SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 682
Score = 28.3 bits (60), Expect = 1.4
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = +1
Query: 457 NLHIPSVAAPGPVWAPSYLKDP*RVPRQNHEHILSSPLAQS 579
N+H PS A + +++P +P+QNHEH SPL +S
Sbjct: 623 NIHHPSANLLDASAALNPVQNPLLMPQQNHEH---SPLVRS 660
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 2.5
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +3
Query: 360 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 449
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 3.3
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 97 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 198
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 4.3
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 291 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 392
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence
orphan|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1563
Score = 26.2 bits (55), Expect = 5.7
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = +3
Query: 639 TQTKPTASTTRLYTTSASALSNCPHPT 719
T T +T + TTS S ++CP PT
Sbjct: 166 TSTSCNPATVLIVTTSGSTSTSCPPPT 192
>SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 363
Score = 26.2 bits (55), Expect = 5.7
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +2
Query: 41 KITYTK*GKSFIYRPANVV 97
+I+YT G SFIYRP N++
Sbjct: 64 EISYT--GTSFIYRPGNII 80
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 10.0
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +3
Query: 312 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 443
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 10.0
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -3
Query: 349 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 254
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
>SPAC32A11.03c |phx1||homeobox transcription factor
Phx1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 942
Score = 25.4 bits (53), Expect = 10.0
Identities = 15/47 (31%), Positives = 20/47 (42%)
Frame = +3
Query: 573 PKYQTLSSNHTMRLSQFTS*LKTQTKPTASTTRLYTTSASALSNCPH 713
PK T H +S T +K+ S+TRL + S S PH
Sbjct: 664 PKTNTEGLMHAQSVSPITQAMKSANVLEGSSTRLNSYEPSVSSAYPH 710
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,295,479
Number of Sequences: 5004
Number of extensions: 66734
Number of successful extensions: 222
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 408446760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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