Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021853
(786 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy... 124 1e-29
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 116 5e-27
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 75 9e-15
SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo... 63 4e-11
SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||... 38 0.002
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 35 0.011
SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1||... 35 0.015
SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|... 34 0.020
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 30 0.43
SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1||... 29 0.76
SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||... 29 1.00
SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|ch... 28 1.3
SPAC23H4.07c |srp102||signal recognition particle receptor beta ... 27 3.0
SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pom... 26 7.0
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 25 9.3
>SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 180
Score = 124 bits (300), Expect = 1e-29
Identities = 56/85 (65%), Positives = 67/85 (78%)
Frame = +1
Query: 256 GKTTILYKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAI 435
GKTTILYKL++GE+VTTIPTIGFNVE V Y+N+ F VWD+GGQ IRP WR Y+ NT I
Sbjct: 29 GKTTILYKLKLGEIVTTIPTIGFNVETVEYRNISFTVWDVGGQDKIRPLWRHYFQNTQGI 88
Query: 436 IYVVDSADRDRIGISKDELVHMLRE 510
I+VVDS DR+RI + +EL ML E
Sbjct: 89 IFVVDSNDRERISEAHEELQRMLNE 113
Score = 60.5 bits (140), Expect = 3e-10
Identities = 28/65 (43%), Positives = 42/65 (64%)
Frame = +3
Query: 510 EELANAILVVLANKQDMAGCLTVAEVHQALGLDALRDRTFQIFKTSAVRGEGLDQAMDWL 689
+EL +A+L+V ANKQD+ + AE+ LGL +LR R + I T A G+GL + ++WL
Sbjct: 114 DELRDALLLVFANKQDLPNAMNAAEITDKLGLHSLRHRQWYIQATCATSGDGLYEGLEWL 173
Query: 690 SNALQ 704
S L+
Sbjct: 174 STNLK 178
Score = 31.1 bits (67), Expect = 0.19
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +2
Query: 197 FRGLLGAREMRILILGLDGAERRQFFINYK 286
F+ L G REMRIL++GLD A + K
Sbjct: 9 FQSLFGKREMRILMVGLDAAGKTTILYKLK 38
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 116 bits (278), Expect = 5e-27
Identities = 51/89 (57%), Positives = 67/89 (75%)
Frame = +1
Query: 256 GKTTILYKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAI 435
GKTTILYKL++ + V TIPT+GFNVE VTYKN+KF VWD+GGQ IRP WR Y+ T +
Sbjct: 33 GKTTILYKLKLNQSVVTIPTVGFNVETVTYKNIKFNVWDVGGQDKIRPLWRHYFTGTKGL 92
Query: 436 IYVVDSADRDRIGISKDELVHMLREKN*R 522
I+VVDSAD +RI ++ EL ++ ++ R
Sbjct: 93 IFVVDSADSNRISEARQELHRIISDREMR 121
Score = 59.3 bits (137), Expect = 6e-10
Identities = 26/64 (40%), Positives = 42/64 (65%)
Frame = +3
Query: 501 VKGEELANAILVVLANKQDMAGCLTVAEVHQALGLDALRDRTFQIFKTSAVRGEGLDQAM 680
+ E+ + +L+VLANKQD+ G L+ A++ L LD L+DR + + T A+ G+GL + +
Sbjct: 115 ISDREMRDCLLLVLANKQDLPGALSPAQITDVLQLDKLKDRLWNVQPTCALTGDGLLEGL 174
Query: 681 DWLS 692
WLS
Sbjct: 175 AWLS 178
Score = 27.1 bits (57), Expect = 3.0
Identities = 14/35 (40%), Positives = 17/35 (48%)
Frame = +2
Query: 182 GLFSYFRGLLGAREMRILILGLDGAERRQFFINYK 286
G F L +EMRIL+LGLD A + K
Sbjct: 8 GFSKPFSRLFSNKEMRILMLGLDAAGKTTILYKLK 42
>SPAC22F3.05c |alp41||ADP-ribosylation factor
Alp41|Schizosaccharomyces pombe|chr 1|||Manual
Length = 186
Score = 75.4 bits (177), Expect = 9e-15
Identities = 35/90 (38%), Positives = 56/90 (62%)
Frame = +1
Query: 244 IGRCGKTTILYKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGN 423
+ GKTTIL L +V PT GF + + + L+F +WD+GGQ ++R +W+ Y+ +
Sbjct: 24 LDNAGKTTILKCLLNEDVNEVSPTFGFQIRTLEVEGLRFTIWDIGGQKTLRNFWKNYFES 83
Query: 424 TDAIIYVVDSADRDRIGISKDELVHMLREK 513
T+AII+VVDS D R+ ++ L +L E+
Sbjct: 84 TEAIIWVVDSLDDLRLEECRNTLQELLVEE 113
>SPBC31F10.06c |sar1||ADP-ribosylation factor
Sar1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 190
Score = 63.3 bits (147), Expect = 4e-11
Identities = 30/87 (34%), Positives = 48/87 (55%)
Frame = +1
Query: 244 IGRCGKTTILYKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGN 423
+ GKTT+L+ L+ + PT+ E++ N++F +DLGG R WR Y+
Sbjct: 28 LDNAGKTTLLHMLKNDRLAVMQPTLHPTSEELAIGNVRFTTFDLGGHQQARRLWRDYFPE 87
Query: 424 TDAIIYVVDSADRDRIGISKDELVHML 504
+ I+Y+VD D +R+ SK EL +L
Sbjct: 88 VNGIVYLVDCCDFERLSESKAELDALL 114
Score = 30.7 bits (66), Expect = 0.25
Identities = 21/71 (29%), Positives = 31/71 (43%), Gaps = 10/71 (14%)
Frame = +3
Query: 510 EELANAILVVLANKQDMAGCLTVAEVHQALGLDALRD----------RTFQIFKTSAVRG 659
EELA ++L NK D G ++ E+ ALGL R ++F S V
Sbjct: 117 EELARVPFLILGNKIDAPGAISEDELKAALGLYQTTGKGVSKPVPGIRPIEVFMCSVVLR 176
Query: 660 EGLDQAMDWLS 692
+G + WL+
Sbjct: 177 QGYGEGFKWLA 187
>SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 203
Score = 37.9 bits (84), Expect = 0.002
Identities = 21/57 (36%), Positives = 27/57 (47%), Gaps = 4/57 (7%)
Frame = +1
Query: 307 IPTIGFNVEQVTY----KNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRD 465
I TIG + + T+ K +K Q+WD GQ R YY II V D D+D
Sbjct: 38 ISTIGVDFKIRTFELEGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQD 94
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 35.1 bits (77), Expect = 0.011
Identities = 16/52 (30%), Positives = 30/52 (57%)
Frame = +1
Query: 313 TIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDR 468
T+G + T +L+ +++D+GGQ + R W + N ++II+ V D D+
Sbjct: 179 TLGISEISFTLDHLQIRMFDVGGQRTERRKWIYCFENVNSIIFCVSLNDYDK 230
>SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 234
Score = 34.7 bits (76), Expect = 0.015
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = +1
Query: 292 EVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRD 465
+ + I ++G +Q K +K Q+WD GQ R R YY + V D ++D
Sbjct: 45 DFASRIISVGMGNQQ---KRIKLQIWDTAGQEKFRSVARNYYRGAAGAVLVYDVTNKD 99
>SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 200
Score = 34.3 bits (75), Expect = 0.020
Identities = 20/74 (27%), Positives = 33/74 (44%), Gaps = 5/74 (6%)
Frame = +1
Query: 256 GKTTILYKLQVGEVVTT-IPTIGFNVE----QVTYKNLKFQVWDLGGQTSIRPYWRCYYG 420
GK+ +L + + I TIG + + ++ K +K Q+WD GQ R YY
Sbjct: 21 GKSCLLLRFSEDSFTPSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYR 80
Query: 421 NTDAIIYVVDSADR 462
I+ + D D+
Sbjct: 81 GAMGILLLYDVTDK 94
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 29.9 bits (64), Expect = 0.43
Identities = 15/54 (27%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
Frame = -1
Query: 546 WLKQRVSRSLILLP*HMHKFI--FRYSYSIPVS*VYNIYYCICVAIVTSPIRPN 391
+L+ R+S+ P +F+ F ++Y++ S VYN + +C + T+P + N
Sbjct: 471 YLRSRLSQFASQSPLFREQFLLNFDFTYNLSESEVYNTVFNLCDELYTTPCKKN 524
>SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr
1|||Manual
Length = 214
Score = 29.1 bits (62), Expect = 0.76
Identities = 20/70 (28%), Positives = 29/70 (41%), Gaps = 5/70 (7%)
Frame = +1
Query: 256 GKTTILYKLQVGEV-VTTIPTIG--FNVEQVTYKN--LKFQVWDLGGQTSIRPYWRCYYG 420
GK+ +L + E + + TIG F + N +K Q+WD GQ R YY
Sbjct: 22 GKSNLLMRFTRNEFNIESKSTIGVEFATRNIVLDNKKIKAQIWDTAGQERYRAITSAYYR 81
Query: 421 NTDAIIYVVD 450
+ V D
Sbjct: 82 GAVGALIVYD 91
>SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr
1|||Manual
Length = 211
Score = 28.7 bits (61), Expect = 1.00
Identities = 13/54 (24%), Positives = 24/54 (44%)
Frame = +1
Query: 349 NLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKDELVHMLRE 510
++K ++WD GQ + YY N + I V D + +K + + R+
Sbjct: 63 SVKLEIWDTAGQERYKSLAPMYYRNANCAIVVYDITQAASLEKAKSWIKELQRQ 116
>SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 198
Score = 28.3 bits (60), Expect = 1.3
Identities = 19/82 (23%), Positives = 37/82 (45%), Gaps = 5/82 (6%)
Frame = +1
Query: 256 GKTTILYKLQVG---EVVTTIPTIGFNVEQVTYKN--LKFQVWDLGGQTSIRPYWRCYYG 420
GKT+++ G E T + F + ++ +N + F +WDLGGQ
Sbjct: 22 GKTSLMVTYVQGSFDEESTQTLGVNFMEKTISIRNTEITFSIWDLGGQREFVNMLPMVCN 81
Query: 421 NTDAIIYVVDSADRDRIGISKD 486
+ AI+++ D + + + K+
Sbjct: 82 DAVAILFMFDLSRKSTLNSIKE 103
>SPAC23H4.07c |srp102||signal recognition particle receptor beta
subunit Srp102 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 27.1 bits (57), Expect = 3.0
Identities = 24/76 (31%), Positives = 38/76 (50%), Gaps = 5/76 (6%)
Frame = +1
Query: 256 GKTTILYKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVW--DLGGQTSIRPYWRCYYG--- 420
GKT++ +L E TT+P+I N E V K+ W DL G + + +
Sbjct: 49 GKTSLFCELIYKEKKTTVPSIEPN-EAV----WKYGAWLVDLPGHPRAKRWITTKFSGNY 103
Query: 421 NTDAIIYVVDSADRDR 468
N A+++V++SA DR
Sbjct: 104 NVKAVVFVLNSATIDR 119
>SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1692
Score = 25.8 bits (54), Expect = 7.0
Identities = 19/72 (26%), Positives = 30/72 (41%)
Frame = -1
Query: 570 DIRPCPVCWLKQRVSRSLILLP*HMHKFIFRYSYSIPVS*VYNIYYCICVAIVTSPIRPN 391
DI + LK V +L+ + + S S +S + N+ Y C S + PN
Sbjct: 665 DISHAKLIGLKDSVIETLVNVETVKVNYNHFTSISDAISAMQNLKYLSCTNCEMSYVSPN 724
Query: 390 TGLSPKIPHLEL 355
G + HL+L
Sbjct: 725 LGKLKHLVHLDL 736
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 25.4 bits (53), Expect = 9.3
Identities = 13/44 (29%), Positives = 20/44 (45%)
Frame = -2
Query: 155 NLLLLIRNVYALRLHIKDF*NCFFVLIYTWSTLNILFNNKYCTF 24
N + +R+ + R HI C ++ WS + L NN Y F
Sbjct: 422 NKCIQLRDYFDYRKHI-----CIVTDLFGWSVFDFLKNNNYIPF 460
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,380,133
Number of Sequences: 5004
Number of extensions: 73073
Number of successful extensions: 173
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 381366860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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