SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021852X
         (639 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p...   134   1e-32
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ...   126   2e-30
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc...   113   3e-26
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M...   107   1e-24
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce...    78   1e-15
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch...    54   2e-08
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom...    44   1e-05
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe...    30   0.25 
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo...    27   1.7  
SPBC1711.16 |||WD repeat protein Pwp1 |Schizosaccharomyces pombe...    26   4.0  
SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    25   7.0  
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar...    25   9.2  
SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom...    25   9.2  
SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyc...    25   9.2  
SPBC776.10c |cog6||Golgi transport complex peripheral subunit Co...    25   9.2  
SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom...    25   9.2  
SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MB...    25   9.2  
SPCC965.03 |vma8||V-type ATPase subunit D |Schizosaccharomyces p...    25   9.2  

>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 647

 Score =  134 bits (324), Expect = 1e-32
 Identities = 62/88 (70%), Positives = 72/88 (81%)
 Frame = +3

Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSM 422
           P   IFDAKRLIGRKF+D  VQ+DMKHWPF+V+S  GKP ++V YKGE KTF PEE+SSM
Sbjct: 61  PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSM 120

Query: 423 VLTKMKETAEAYLGKTVQNAVITVPACF 506
           VL KM+ETAEAYLG  V +AV+TVPA F
Sbjct: 121 VLMKMRETAEAYLGGKVTDAVVTVPAYF 148



 Score =  117 bits (281), Expect = 2e-27
 Identities = 53/61 (86%), Positives = 58/61 (95%)
 Frame = +1

Query: 73  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 252
           ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N
Sbjct: 4   SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63

Query: 253 T 255
           T
Sbjct: 64  T 64



 Score = 76.2 bits (179), Expect = 4e-15
 Identities = 36/43 (83%), Positives = 38/43 (88%)
 Frame = +2

Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERN 637
           DSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+   GE N
Sbjct: 150 DSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESN 192


>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 644

 Score =  126 bits (305), Expect = 2e-30
 Identities = 58/88 (65%), Positives = 70/88 (79%)
 Frame = +3

Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSM 422
           P   IFDAKRLIGR+F D  VQ+DMKHWPF+V+   GKP I+V +KGE KTF PEE+SSM
Sbjct: 61  PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSM 120

Query: 423 VLTKMKETAEAYLGKTVQNAVITVPACF 506
           VL KM+E+AEA+LG  V +AV+TVPA F
Sbjct: 121 VLLKMRESAEAFLGGKVTDAVVTVPAYF 148



 Score =  117 bits (281), Expect = 2e-27
 Identities = 53/61 (86%), Positives = 58/61 (95%)
 Frame = +1

Query: 73  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 252
           ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N
Sbjct: 4   SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63

Query: 253 T 255
           T
Sbjct: 64  T 64



 Score = 72.9 bits (171), Expect = 4e-14
 Identities = 35/43 (81%), Positives = 37/43 (86%)
 Frame = +2

Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERN 637
           DSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+    E N
Sbjct: 150 DSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETN 192


>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 613

 Score =  113 bits (271), Expect = 3e-26
 Identities = 52/88 (59%), Positives = 65/88 (73%)
 Frame = +3

Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSM 422
           P+  +FDAKRLIGR++ED   Q D+KHWPF+V+   G P I+V Y GE K F  +E+S+M
Sbjct: 65  PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124

Query: 423 VLTKMKETAEAYLGKTVQNAVITVPACF 506
           VLTKMKE +EA L K V+ AVITVPA F
Sbjct: 125 VLTKMKEISEAKLNKRVEKAVITVPAYF 152



 Score =  104 bits (250), Expect = 9e-24
 Identities = 47/61 (77%), Positives = 53/61 (86%)
 Frame = +1

Query: 73  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 252
           A+GIDLGTTYSCV V++   VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP N
Sbjct: 8   AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67

Query: 253 T 255
           T
Sbjct: 68  T 68



 Score = 70.5 bits (165), Expect = 2e-13
 Identities = 34/43 (79%), Positives = 36/43 (83%)
 Frame = +2

Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERN 637
           DSQR ATKDAG I+GLNVLRIINEPTAAAIAYGLD K    +N
Sbjct: 154 DSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKN 196


>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 663

 Score =  107 bits (258), Expect = 1e-24
 Identities = 49/97 (50%), Positives = 70/97 (72%)
 Frame = +3

Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSM 422
           P+  IFD KRLIGRKF++ T+  D+K +PF +V+   +P ++V   G+ K F PEE+S+M
Sbjct: 94  PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153

Query: 423 VLTKMKETAEAYLGKTVQNAVITVPACFKTLKDKPQK 533
           +L+KMK+TAEAYLGK V +AV+TVPA F   + +  K
Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATK 190



 Score =  102 bits (245), Expect = 4e-23
 Identities = 45/60 (75%), Positives = 52/60 (86%)
 Frame = +1

Query: 76  VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNT 255
           +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ   NP NT
Sbjct: 38  IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97



 Score = 72.5 bits (170), Expect = 5e-14
 Identities = 33/39 (84%), Positives = 37/39 (94%)
 Frame = +2

Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGT 625
           D+QRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLDK  T
Sbjct: 183 DAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDT 221


>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 674

 Score = 77.8 bits (183), Expect = 1e-15
 Identities = 36/66 (54%), Positives = 47/66 (71%), Gaps = 1/66 (1%)
 Frame = +1

Query: 64  KHPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 240
           K P +GIDLGTT SC+ + +    ++IAN +G RTTPS VAFT D ERL+G +AK Q  +
Sbjct: 49  KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108

Query: 241 NPNNTY 258
           NP NT+
Sbjct: 109 NPENTF 114



 Score = 77.8 bits (183), Expect = 1e-15
 Identities = 37/88 (42%), Positives = 54/88 (61%)
 Frame = +3

Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSM 422
           P+   F  KRLIGR+F++  VQ D+K  P+++V            +   KT+ P ++   
Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGGF 166

Query: 423 VLTKMKETAEAYLGKTVQNAVITVPACF 506
           +L+KM+ETA  YLGK V+NAV+TVPA F
Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAYF 194



 Score = 68.9 bits (161), Expect = 6e-13
 Identities = 31/37 (83%), Positives = 35/37 (94%)
 Frame = +2

Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 619
           DSQRQATK AG I+GLNVLR++NEPTAAA+AYGLDKK
Sbjct: 196 DSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK 232


>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
           Pss1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 720

 Score = 53.6 bits (123), Expect = 2e-08
 Identities = 23/60 (38%), Positives = 39/60 (65%)
 Frame = +1

Query: 76  VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNT 255
           VGID G + + + V ++  +++I N+  NR+TPS V++ +  R +G+AAK+  A N  NT
Sbjct: 8   VGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEASNFRNT 67



 Score = 41.1 bits (92), Expect = 1e-04
 Identities = 18/36 (50%), Positives = 26/36 (72%)
 Frame = +2

Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDK 616
           D QR+A  +A  I+GLN LR++N+  AAA+ YG+ K
Sbjct: 154 DIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITK 189



 Score = 39.9 bits (89), Expect = 3e-04
 Identities = 25/85 (29%), Positives = 43/85 (50%), Gaps = 1/85 (1%)
 Frame = +3

Query: 267 KRLIGRKFEDATVQ-ADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 443
           KRL GR ++D  ++  +      ++    G    KV Y  E+  F   ++ +   TK+K 
Sbjct: 72  KRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAYFTKIKA 131

Query: 444 TAEAYLGKTVQNAVITVPACFKTLK 518
            AEA L  +V + VI++PA F  ++
Sbjct: 132 IAEAELIGSVSDVVISIPAWFTDIQ 156


>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 566

 Score = 44.4 bits (100), Expect = 1e-05
 Identities = 18/60 (30%), Positives = 34/60 (56%)
 Frame = +1

Query: 76  VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNT 255
           +GI  G   S +   + GK +++AN++GNR  PS +++   +   G  A+ Q+  N +N+
Sbjct: 27  IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADNS 86



 Score = 29.5 bits (63), Expect = 0.43
 Identities = 15/47 (31%), Positives = 24/47 (51%)
 Frame = +3

Query: 384 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPACFKTLKDK 524
           ++K     E S   L ++ E+AE +LG  V   V++VP  F   + K
Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRK 180


>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 848

 Score = 30.3 bits (65), Expect = 0.25
 Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%)
 Frame = +1

Query: 76  VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 252
           + ID GT ++   + + G  +EI+      R   S VAF   ER+ G  A N     P  
Sbjct: 25  LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFP-- 82

Query: 253 TYSMPNV 273
            +S+ NV
Sbjct: 83  AHSIRNV 89


>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2244

 Score = 27.5 bits (58), Expect = 1.7
 Identities = 14/45 (31%), Positives = 24/45 (53%)
 Frame = +1

Query: 172  PSYVAFTDTERLIGDAAKNQVAMNPNNTYSMPNVSSDVSSKMLLC 306
            P  +A T  ER++  AAK   A+N    Y++  ++ D   K++ C
Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296


>SPBC1711.16 |||WD repeat protein Pwp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 516

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 21/72 (29%), Positives = 33/72 (45%), Gaps = 11/72 (15%)
 Frame = +1

Query: 28  QVTRLIKNTKWQKHPA----VGIDLGTTYSCVGVFQHGKV-EIIAND------QGNRTTP 174
           QVT  ++N  W +H      +G D G  Y C        V ++ A+D        N + P
Sbjct: 337 QVTSDVENVAWDQHSENNFFIGTDNGIVYYCDARNLSKSVWQLQAHDGPISCLSVNPSVP 396

Query: 175 SYVAFTDTERLI 210
           S+VA   T+R++
Sbjct: 397 SFVATGSTDRVV 408


>SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 600

 Score = 25.4 bits (53), Expect = 7.0
 Identities = 14/35 (40%), Positives = 19/35 (54%)
 Frame = +1

Query: 157 GNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNTYS 261
           GNR+T ++        L  DAAKN +A   + TYS
Sbjct: 349 GNRSTTNFSIINSGLYLKKDAAKNLLAKQFDATYS 383


>SPAC25B8.04c |||mitochondrial splicing suppressor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 378

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 10/27 (37%), Positives = 16/27 (59%)
 Frame = +2

Query: 83  SIWVPRTLALVSSSTGRWRSSPTTRAT 163
           S W+P    LVS+    W +SP+++ T
Sbjct: 288 SSWIPTLHDLVSTRCSVWLTSPSSQRT 314


>SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces
            pombe|chr 1||Partial|Manual
          Length = 1887

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 11/38 (28%), Positives = 19/38 (50%)
 Frame = -3

Query: 544  GTCIFCGLSLRVLKHAGTVITAFCTVLPR*ASAVSFIF 431
            G C+FCGL+      +    T+ C    R A+ +S ++
Sbjct: 1827 GLCLFCGLTKFEHADSDMAYTSDCRSWARKANLISLVY 1864


>SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1428

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 13/38 (34%), Positives = 18/38 (47%)
 Frame = -2

Query: 569 FGERSSLRWYLHLLWLVFESLEARGNRDNCILHSFAEI 456
           F  RSS       LW  F SL+  G  ++ I  +F+ I
Sbjct: 143 FSNRSSFSLRKRYLWTTFLSLKGMGFEESEIFGAFSSI 180


>SPBC776.10c |cog6||Golgi transport complex peripheral subunit Cog6
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 675

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 10/28 (35%), Positives = 16/28 (57%)
 Frame = +1

Query: 217 AAKNQVAMNPNNTYSMPNVSSDVSSKML 300
           A K Q+ +NP   + +PNV  D  + +L
Sbjct: 323 ARKRQIQLNPFPPWDVPNVLEDQMNSLL 350


>SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1919

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 11/38 (28%), Positives = 19/38 (50%)
 Frame = -3

Query: 544  GTCIFCGLSLRVLKHAGTVITAFCTVLPR*ASAVSFIF 431
            G C+FCGL+      +    T+ C    R A+ +S ++
Sbjct: 1827 GLCLFCGLTKFEHADSDMAYTSDCRSWARKANLISLVY 1864


>SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MBOAT
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 588

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 9/37 (24%), Positives = 18/37 (48%)
 Frame = -2

Query: 545 WYLHLLWLVFESLEARGNRDNCILHSFAEISFGSFLH 435
           W L  LW + + +E   N   C+ ++++ + F    H
Sbjct: 396 WRLFRLWSLIDDIEPPENIVRCMCNNYSAVGFWRAWH 432


>SPCC965.03 |vma8||V-type ATPase subunit D |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 285

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 9/25 (36%), Positives = 17/25 (68%)
 Frame = +1

Query: 208 IGDAAKNQVAMNPNNTYSMPNVSSD 282
           +G    N+V  + ++TYS+P+ S+D
Sbjct: 248 VGKEVINEVENSKDDTYSLPSTSTD 272


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,748,716
Number of Sequences: 5004
Number of extensions: 57945
Number of successful extensions: 219
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 217
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -