BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021852X
(639 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 134 1e-32
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 126 2e-30
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 113 3e-26
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 107 1e-24
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 78 1e-15
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 54 2e-08
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 44 1e-05
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 30 0.25
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 27 1.7
SPBC1711.16 |||WD repeat protein Pwp1 |Schizosaccharomyces pombe... 26 4.0
SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 7.0
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 25 9.2
SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 25 9.2
SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyc... 25 9.2
SPBC776.10c |cog6||Golgi transport complex peripheral subunit Co... 25 9.2
SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 25 9.2
SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MB... 25 9.2
SPCC965.03 |vma8||V-type ATPase subunit D |Schizosaccharomyces p... 25 9.2
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 134 bits (324), Expect = 1e-32
Identities = 62/88 (70%), Positives = 72/88 (81%)
Frame = +3
Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSM 422
P IFDAKRLIGRKF+D VQ+DMKHWPF+V+S GKP ++V YKGE KTF PEE+SSM
Sbjct: 61 PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSM 120
Query: 423 VLTKMKETAEAYLGKTVQNAVITVPACF 506
VL KM+ETAEAYLG V +AV+TVPA F
Sbjct: 121 VLMKMRETAEAYLGGKVTDAVVTVPAYF 148
Score = 117 bits (281), Expect = 2e-27
Identities = 53/61 (86%), Positives = 58/61 (95%)
Frame = +1
Query: 73 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 252
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63
Query: 253 T 255
T
Sbjct: 64 T 64
Score = 76.2 bits (179), Expect = 4e-15
Identities = 36/43 (83%), Positives = 38/43 (88%)
Frame = +2
Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERN 637
DSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ GE N
Sbjct: 150 DSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESN 192
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 126 bits (305), Expect = 2e-30
Identities = 58/88 (65%), Positives = 70/88 (79%)
Frame = +3
Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSM 422
P IFDAKRLIGR+F D VQ+DMKHWPF+V+ GKP I+V +KGE KTF PEE+SSM
Sbjct: 61 PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSM 120
Query: 423 VLTKMKETAEAYLGKTVQNAVITVPACF 506
VL KM+E+AEA+LG V +AV+TVPA F
Sbjct: 121 VLLKMRESAEAFLGGKVTDAVVTVPAYF 148
Score = 117 bits (281), Expect = 2e-27
Identities = 53/61 (86%), Positives = 58/61 (95%)
Frame = +1
Query: 73 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 252
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63
Query: 253 T 255
T
Sbjct: 64 T 64
Score = 72.9 bits (171), Expect = 4e-14
Identities = 35/43 (81%), Positives = 37/43 (86%)
Frame = +2
Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERN 637
DSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ E N
Sbjct: 150 DSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETN 192
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 113 bits (271), Expect = 3e-26
Identities = 52/88 (59%), Positives = 65/88 (73%)
Frame = +3
Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSM 422
P+ +FDAKRLIGR++ED Q D+KHWPF+V+ G P I+V Y GE K F +E+S+M
Sbjct: 65 PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124
Query: 423 VLTKMKETAEAYLGKTVQNAVITVPACF 506
VLTKMKE +EA L K V+ AVITVPA F
Sbjct: 125 VLTKMKEISEAKLNKRVEKAVITVPAYF 152
Score = 104 bits (250), Expect = 9e-24
Identities = 47/61 (77%), Positives = 53/61 (86%)
Frame = +1
Query: 73 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 252
A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP N
Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67
Query: 253 T 255
T
Sbjct: 68 T 68
Score = 70.5 bits (165), Expect = 2e-13
Identities = 34/43 (79%), Positives = 36/43 (83%)
Frame = +2
Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERN 637
DSQR ATKDAG I+GLNVLRIINEPTAAAIAYGLD K +N
Sbjct: 154 DSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKN 196
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 107 bits (258), Expect = 1e-24
Identities = 49/97 (50%), Positives = 70/97 (72%)
Frame = +3
Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSM 422
P+ IFD KRLIGRKF++ T+ D+K +PF +V+ +P ++V G+ K F PEE+S+M
Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153
Query: 423 VLTKMKETAEAYLGKTVQNAVITVPACFKTLKDKPQK 533
+L+KMK+TAEAYLGK V +AV+TVPA F + + K
Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATK 190
Score = 102 bits (245), Expect = 4e-23
Identities = 45/60 (75%), Positives = 52/60 (86%)
Frame = +1
Query: 76 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNT 255
+GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ NP NT
Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97
Score = 72.5 bits (170), Expect = 5e-14
Identities = 33/39 (84%), Positives = 37/39 (94%)
Frame = +2
Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGT 625
D+QRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLDK T
Sbjct: 183 DAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDT 221
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 77.8 bits (183), Expect = 1e-15
Identities = 36/66 (54%), Positives = 47/66 (71%), Gaps = 1/66 (1%)
Frame = +1
Query: 64 KHPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 240
K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q +
Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108
Query: 241 NPNNTY 258
NP NT+
Sbjct: 109 NPENTF 114
Score = 77.8 bits (183), Expect = 1e-15
Identities = 37/88 (42%), Positives = 54/88 (61%)
Frame = +3
Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSM 422
P+ F KRLIGR+F++ VQ D+K P+++V + KT+ P ++
Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGGF 166
Query: 423 VLTKMKETAEAYLGKTVQNAVITVPACF 506
+L+KM+ETA YLGK V+NAV+TVPA F
Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAYF 194
Score = 68.9 bits (161), Expect = 6e-13
Identities = 31/37 (83%), Positives = 35/37 (94%)
Frame = +2
Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 619
DSQRQATK AG I+GLNVLR++NEPTAAA+AYGLDKK
Sbjct: 196 DSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK 232
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 53.6 bits (123), Expect = 2e-08
Identities = 23/60 (38%), Positives = 39/60 (65%)
Frame = +1
Query: 76 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNT 255
VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A N NT
Sbjct: 8 VGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEASNFRNT 67
Score = 41.1 bits (92), Expect = 1e-04
Identities = 18/36 (50%), Positives = 26/36 (72%)
Frame = +2
Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDK 616
D QR+A +A I+GLN LR++N+ AAA+ YG+ K
Sbjct: 154 DIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITK 189
Score = 39.9 bits (89), Expect = 3e-04
Identities = 25/85 (29%), Positives = 43/85 (50%), Gaps = 1/85 (1%)
Frame = +3
Query: 267 KRLIGRKFEDATVQ-ADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 443
KRL GR ++D ++ + ++ G KV Y E+ F ++ + TK+K
Sbjct: 72 KRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAYFTKIKA 131
Query: 444 TAEAYLGKTVQNAVITVPACFKTLK 518
AEA L +V + VI++PA F ++
Sbjct: 132 IAEAELIGSVSDVVISIPAWFTDIQ 156
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 44.4 bits (100), Expect = 1e-05
Identities = 18/60 (30%), Positives = 34/60 (56%)
Frame = +1
Query: 76 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNT 255
+GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ N +N+
Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADNS 86
Score = 29.5 bits (63), Expect = 0.43
Identities = 15/47 (31%), Positives = 24/47 (51%)
Frame = +3
Query: 384 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPACFKTLKDK 524
++K E S L ++ E+AE +LG V V++VP F + K
Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRK 180
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 30.3 bits (65), Expect = 0.25
Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%)
Frame = +1
Query: 76 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 252
+ ID GT ++ + + G +EI+ R S VAF ER+ G A N P
Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFP-- 82
Query: 253 TYSMPNV 273
+S+ NV
Sbjct: 83 AHSIRNV 89
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2244
Score = 27.5 bits (58), Expect = 1.7
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = +1
Query: 172 PSYVAFTDTERLIGDAAKNQVAMNPNNTYSMPNVSSDVSSKMLLC 306
P +A T ER++ AAK A+N Y++ ++ D K++ C
Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296
>SPBC1711.16 |||WD repeat protein Pwp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 516
Score = 26.2 bits (55), Expect = 4.0
Identities = 21/72 (29%), Positives = 33/72 (45%), Gaps = 11/72 (15%)
Frame = +1
Query: 28 QVTRLIKNTKWQKHPA----VGIDLGTTYSCVGVFQHGKV-EIIAND------QGNRTTP 174
QVT ++N W +H +G D G Y C V ++ A+D N + P
Sbjct: 337 QVTSDVENVAWDQHSENNFFIGTDNGIVYYCDARNLSKSVWQLQAHDGPISCLSVNPSVP 396
Query: 175 SYVAFTDTERLI 210
S+VA T+R++
Sbjct: 397 SFVATGSTDRVV 408
>SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 600
Score = 25.4 bits (53), Expect = 7.0
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +1
Query: 157 GNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNTYS 261
GNR+T ++ L DAAKN +A + TYS
Sbjct: 349 GNRSTTNFSIINSGLYLKKDAAKNLLAKQFDATYS 383
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 25.0 bits (52), Expect = 9.2
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +2
Query: 83 SIWVPRTLALVSSSTGRWRSSPTTRAT 163
S W+P LVS+ W +SP+++ T
Sbjct: 288 SSWIPTLHDLVSTRCSVWLTSPSSQRT 314
>SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces
pombe|chr 1||Partial|Manual
Length = 1887
Score = 25.0 bits (52), Expect = 9.2
Identities = 11/38 (28%), Positives = 19/38 (50%)
Frame = -3
Query: 544 GTCIFCGLSLRVLKHAGTVITAFCTVLPR*ASAVSFIF 431
G C+FCGL+ + T+ C R A+ +S ++
Sbjct: 1827 GLCLFCGLTKFEHADSDMAYTSDCRSWARKANLISLVY 1864
>SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1428
Score = 25.0 bits (52), Expect = 9.2
Identities = 13/38 (34%), Positives = 18/38 (47%)
Frame = -2
Query: 569 FGERSSLRWYLHLLWLVFESLEARGNRDNCILHSFAEI 456
F RSS LW F SL+ G ++ I +F+ I
Sbjct: 143 FSNRSSFSLRKRYLWTTFLSLKGMGFEESEIFGAFSSI 180
>SPBC776.10c |cog6||Golgi transport complex peripheral subunit Cog6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 675
Score = 25.0 bits (52), Expect = 9.2
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +1
Query: 217 AAKNQVAMNPNNTYSMPNVSSDVSSKML 300
A K Q+ +NP + +PNV D + +L
Sbjct: 323 ARKRQIQLNPFPPWDVPNVLEDQMNSLL 350
>SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1919
Score = 25.0 bits (52), Expect = 9.2
Identities = 11/38 (28%), Positives = 19/38 (50%)
Frame = -3
Query: 544 GTCIFCGLSLRVLKHAGTVITAFCTVLPR*ASAVSFIF 431
G C+FCGL+ + T+ C R A+ +S ++
Sbjct: 1827 GLCLFCGLTKFEHADSDMAYTSDCRSWARKANLISLVY 1864
>SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MBOAT
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 588
Score = 25.0 bits (52), Expect = 9.2
Identities = 9/37 (24%), Positives = 18/37 (48%)
Frame = -2
Query: 545 WYLHLLWLVFESLEARGNRDNCILHSFAEISFGSFLH 435
W L LW + + +E N C+ ++++ + F H
Sbjct: 396 WRLFRLWSLIDDIEPPENIVRCMCNNYSAVGFWRAWH 432
>SPCC965.03 |vma8||V-type ATPase subunit D |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 285
Score = 25.0 bits (52), Expect = 9.2
Identities = 9/25 (36%), Positives = 17/25 (68%)
Frame = +1
Query: 208 IGDAAKNQVAMNPNNTYSMPNVSSD 282
+G N+V + ++TYS+P+ S+D
Sbjct: 248 VGKEVINEVENSKDDTYSLPSTSTD 272
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,748,716
Number of Sequences: 5004
Number of extensions: 57945
Number of successful extensions: 219
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 217
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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