BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021852X (639 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 132 2e-31 SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 132 3e-31 SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 130 9e-31 SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 113 9e-26 SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 69 2e-12 SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 66 2e-11 SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 54 1e-07 SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 6e-04 SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 36 0.037 SB_56988| Best HMM Match : DUF229 (HMM E-Value=2e-06) 34 0.085 SB_4173| Best HMM Match : No HMM Matches (HMM E-Value=.) 34 0.085 SB_55989| Best HMM Match : zf-C2H2 (HMM E-Value=8e-08) 31 0.79 SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.79 SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 2.4 SB_24762| Best HMM Match : Astacin (HMM E-Value=0) 28 7.4 SB_30795| Best HMM Match : Epimerase (HMM E-Value=0) 27 9.7 SB_2538| Best HMM Match : YTV (HMM E-Value=0.0015) 27 9.7 SB_53017| Best HMM Match : Kazal_1 (HMM E-Value=0) 27 9.7 SB_51898| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 9.7 SB_16515| Best HMM Match : Extensin_2 (HMM E-Value=0.12) 27 9.7 >SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) Length = 640 Score = 132 bits (320), Expect = 2e-31 Identities = 62/88 (70%), Positives = 74/88 (84%) Frame = +3 Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSM 422 P+ IFDAKRLIGR+++DATVQ+DMK WPF+++S KPKI+V YKGE KTF EE+SSM Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122 Query: 423 VLTKMKETAEAYLGKTVQNAVITVPACF 506 VL KMKETAEAYLG+ V +AVITVPA F Sbjct: 123 VLGKMKETAEAYLGQKVTSAVITVPAYF 150 Score = 127 bits (306), Expect = 9e-30 Identities = 57/64 (89%), Positives = 61/64 (95%) Frame = +1 Query: 64 KHPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMN 243 K PA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA+N Sbjct: 3 KAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVALN 62 Query: 244 PNNT 255 P NT Sbjct: 63 PENT 66 Score = 79.0 bits (186), Expect = 3e-15 Identities = 36/43 (83%), Positives = 41/43 (95%) Frame = +2 Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERN 637 DSQRQATKDAGTI+GLNVLR+INEPTAAA+AYGLDK +GE+N Sbjct: 152 DSQRQATKDAGTIAGLNVLRVINEPTAAALAYGLDKSLSGEKN 194 >SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) Length = 1097 Score = 132 bits (318), Expect = 3e-31 Identities = 61/64 (95%), Positives = 62/64 (96%) Frame = +1 Query: 64 KHPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMN 243 K PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVAMN Sbjct: 3 KTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVAMN 62 Query: 244 PNNT 255 P NT Sbjct: 63 PENT 66 Score = 130 bits (314), Expect = 9e-31 Identities = 59/88 (67%), Positives = 70/88 (79%) Frame = +3 Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSM 422 P+ +FDAKRLIGRKF+D V DM HWPF V+ +PKI+V +KGE K+FFPEE+SSM Sbjct: 63 PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122 Query: 423 VLTKMKETAEAYLGKTVQNAVITVPACF 506 VLTKMKETAEAYLG V +AV+TVPA F Sbjct: 123 VLTKMKETAEAYLGAKVTDAVVTVPAYF 150 Score = 79.0 bits (186), Expect = 3e-15 Identities = 39/43 (90%), Positives = 40/43 (93%) Frame = +2 Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERN 637 DSQRQATKDAGTI+GLNVLRIINEPTAAAIAYGLDKK ERN Sbjct: 152 DSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGVERN 194 >SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 746 Score = 130 bits (314), Expect = 9e-31 Identities = 59/64 (92%), Positives = 61/64 (95%) Frame = +1 Query: 64 KHPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMN 243 K PA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVAMN Sbjct: 3 KAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVAMN 62 Query: 244 PNNT 255 P NT Sbjct: 63 PTNT 66 Score = 108 bits (260), Expect = 3e-24 Identities = 63/113 (55%), Positives = 71/113 (62%), Gaps = 25/113 (22%) Frame = +3 Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEV--- 413 P IFDAKRLIGR+F+D V+ DMKHW FEVV+ G+PK+KV YKGE KTFF EEV Sbjct: 63 PTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVVNE 122 Query: 414 ----------------------SSMVLTKMKETAEAYLGKTVQNAVITVPACF 506 SSMVL KMKETAEAYLG V +AV+TVPA F Sbjct: 123 AGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYF 175 Score = 78.6 bits (185), Expect = 4e-15 Identities = 39/43 (90%), Positives = 39/43 (90%) Frame = +2 Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERN 637 DSQRQATKDAG ISGLNVLRIINEPTAAAIAYGLDKK ERN Sbjct: 245 DSQRQATKDAGVISGLNVLRIINEPTAAAIAYGLDKKVGQERN 287 Score = 50.8 bits (116), Expect = 9e-07 Identities = 24/33 (72%), Positives = 28/33 (84%) Frame = +3 Query: 408 EVSSMVLTKMKETAEAYLGKTVQNAVITVPACF 506 ++SSMVL KMKETAEAYLG V +AV+TVPA F Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAYF 243 >SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) Length = 1327 Score = 113 bits (273), Expect = 9e-26 Identities = 55/97 (56%), Positives = 71/97 (73%) Frame = +3 Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSSM 422 P+ +FDAKRLIGR ++D +VQ D+K +PF+VV KP ++V K E KTF EE+S+M Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820 Query: 423 VLTKMKETAEAYLGKTVQNAVITVPACFKTLKDKPQK 533 VLTKMKETAEAYLGK V +AV+TVPA F + + K Sbjct: 821 VLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATK 857 Score = 104 bits (249), Expect = 7e-23 Identities = 48/61 (78%), Positives = 54/61 (88%), Gaps = 1/61 (1%) Frame = +1 Query: 76 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNPNN 252 +GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP N Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPEN 763 Query: 253 T 255 T Sbjct: 764 T 764 Score = 71.3 bits (167), Expect = 6e-13 Identities = 34/43 (79%), Positives = 39/43 (90%) Frame = +2 Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERN 637 D+QRQATKDAG I+GL V+RIINEPTAAAIAYG+DKK GE+N Sbjct: 850 DAQRQATKDAGAIAGLTVMRIINEPTAAAIAYGMDKK-EGEKN 891 >SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) Length = 437 Score = 69.3 bits (162), Expect = 2e-12 Identities = 31/66 (46%), Positives = 45/66 (68%), Gaps = 2/66 (3%) Frame = +1 Query: 64 KHPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 237 K +GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89 Query: 238 MNPNNT 255 +NP NT Sbjct: 90 INPKNT 95 Score = 52.4 bits (120), Expect = 3e-07 Identities = 32/89 (35%), Positives = 44/89 (49%), Gaps = 1/89 (1%) Frame = +3 Query: 243 PQQHIFDAKRLIGRKFEDATVQADMKHWPFEVVSGX-GKPKIKVAYKGEDKTFFPEEVSS 419 P+ I+DAKR IG+ F + K + F V + G V KG PEE+ Sbjct: 92 PKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGG 151 Query: 420 MVLTKMKETAEAYLGKTVQNAVITVPACF 506 V+ K++ AE L TV AV++VPA F Sbjct: 152 YVIKKLRTMAERNLTTTVTKAVMSVPAEF 180 Score = 52.0 bits (119), Expect = 4e-07 Identities = 23/35 (65%), Positives = 29/35 (82%) Frame = +2 Query: 515 QRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 619 QR AT A +++G+ VLR+INEPTAAA+AYGL KK Sbjct: 184 QRNATVKAASLAGITVLRVINEPTAAALAYGLHKK 218 >SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1068 Score = 66.5 bits (155), Expect = 2e-11 Identities = 34/81 (41%), Positives = 50/81 (61%), Gaps = 1/81 (1%) Frame = +3 Query: 267 KRLIGRKFEDATVQADMKHWPFEVVSGXGKP-KIKVAYKGEDKTFFPEEVSSMVLTKMKE 443 KR IGRKF D VQ ++ H P++VV I+V Y G+ + F PE+V +M+ T++K Sbjct: 127 KRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAMLFTRLKT 186 Query: 444 TAEAYLGKTVQNAVITVPACF 506 TAE L V + VI+VP+ + Sbjct: 187 TAEIALKTKVTDCVISVPSYY 207 Score = 49.6 bits (113), Expect = 2e-06 Identities = 22/52 (42%), Positives = 33/52 (63%) Frame = +1 Query: 76 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 231 VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55 Score = 40.7 bits (91), Expect = 0.001 Identities = 17/37 (45%), Positives = 26/37 (70%) Frame = +2 Query: 509 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 619 D QR+ DA +GLN LR++N+ TA ++AYG+ K+ Sbjct: 209 DRQRRCMLDASATAGLNCLRLMNDTTAVSLAYGIYKQ 245 >SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) Length = 179 Score = 53.6 bits (123), Expect = 1e-07 Identities = 26/57 (45%), Positives = 39/57 (68%) Frame = +3 Query: 363 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPACFKTLKDKPQK 533 I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA F + + K Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHFNDAQRQATK 58 Score = 50.4 bits (115), Expect = 1e-06 Identities = 37/69 (53%), Positives = 40/69 (57%), Gaps = 26/69 (37%) Frame = +2 Query: 509 DSQRQATKDAGTISGLNVL------------------------RIINEPTAAAIAYGLDK 616 D+QRQATKDAGTI+GL+VL RIINEPTAAAIAYGLDK Sbjct: 51 DAQRQATKDAGTIAGLDVLSRTASERPPSRFRRRRPHGRSRVLRIINEPTAAAIAYGLDK 110 Query: 617 K--GTGERN 637 G ERN Sbjct: 111 ARGGKAERN 119 >SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) Length = 592 Score = 41.5 bits (93), Expect = 6e-04 Identities = 18/28 (64%), Positives = 22/28 (78%) Frame = +1 Query: 127 GKVEIIANDQGNRTTPSYVAFTDTERLI 210 G IIAND G+R TP+ VAF+DTE+LI Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLI 110 >SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1019 Score = 35.5 bits (78), Expect = 0.037 Identities = 17/50 (34%), Positives = 27/50 (54%) Frame = +3 Query: 357 PKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPACF 506 P +K Y+ D TF PEE+ M+L + E + +++ V+TVP F Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFF 116 Score = 34.3 bits (75), Expect = 0.085 Identities = 16/33 (48%), Positives = 23/33 (69%) Frame = +2 Query: 512 SQRQATKDAGTISGLNVLRIINEPTAAAIAYGL 610 ++R+A A + GLNVL+I+N TA A+ YGL Sbjct: 119 AERRALLRAAELVGLNVLQIMNSNTAVALNYGL 151 >SB_56988| Best HMM Match : DUF229 (HMM E-Value=2e-06) Length = 286 Score = 34.3 bits (75), Expect = 0.085 Identities = 18/41 (43%), Positives = 25/41 (60%), Gaps = 2/41 (4%) Frame = +1 Query: 457 ISAKLCRM-QLSRFPRASRLSKTSHKR-CRYHLRLERSPNH 573 +SAKLC + LS+ RA + HKR C Y +++E SP H Sbjct: 198 LSAKLCHVVTLSKIVRALQTHSHLHKRQCSYQIQIETSPAH 238 >SB_4173| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 743 Score = 34.3 bits (75), Expect = 0.085 Identities = 18/41 (43%), Positives = 25/41 (60%), Gaps = 2/41 (4%) Frame = +1 Query: 457 ISAKLCRM-QLSRFPRASRLSKTSHKR-CRYHLRLERSPNH 573 +SAKLC + LS+ RA + HKR C Y +++E SP H Sbjct: 655 LSAKLCHVITLSKIVRALQTHSHLHKRQCSYQIQIETSPAH 695 >SB_55989| Best HMM Match : zf-C2H2 (HMM E-Value=8e-08) Length = 1011 Score = 31.1 bits (67), Expect = 0.79 Identities = 18/62 (29%), Positives = 27/62 (43%) Frame = +3 Query: 354 KPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPACFKTLKDKPQK 533 +PK K + E+ EE +M ++K LGK V+ + T C TL + Q Sbjct: 654 QPKFKKTKEEEEFDKLLEEADAMTQQEVKNVKNIKLGKNVKTQLYTCEPCNITLNSQKQM 713 Query: 534 MQ 539 Q Sbjct: 714 SQ 715 >SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) Length = 212 Score = 31.1 bits (67), Expect = 0.79 Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%) Frame = +1 Query: 136 EIIANDQGNRTTPSYVAFT-DTERL 207 ++I N +G+RTTPS VAFT + ERL Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31 >SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) Length = 350 Score = 29.5 bits (63), Expect = 2.4 Identities = 16/33 (48%), Positives = 19/33 (57%) Frame = -3 Query: 244 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 146 GS PPG W R +AR T +T +SC PG W Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236 >SB_24762| Best HMM Match : Astacin (HMM E-Value=0) Length = 733 Score = 27.9 bits (59), Expect = 7.4 Identities = 10/29 (34%), Positives = 17/29 (58%) Frame = -2 Query: 539 LHLLWLVFESLEARGNRDNCILHSFAEIS 453 ++ LWL F + + D C+LHS A ++ Sbjct: 415 IYTLWLDFSRYYSSASSDICVLHSIAHLA 443 >SB_30795| Best HMM Match : Epimerase (HMM E-Value=0) Length = 869 Score = 27.5 bits (58), Expect = 9.7 Identities = 14/38 (36%), Positives = 18/38 (47%) Frame = +3 Query: 24 RTSNKAD*KYKMAKAPGSRNRSGYHVLLRWCLPAREGG 137 R++ K+D +K KAP G RW PA E G Sbjct: 558 RSAEKSDAIHKEKKAPRGGRSGGPSQRKRWTAPAEEDG 595 >SB_2538| Best HMM Match : YTV (HMM E-Value=0.0015) Length = 779 Score = 27.5 bits (58), Expect = 9.7 Identities = 18/72 (25%), Positives = 36/72 (50%) Frame = +1 Query: 70 PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPN 249 P G+DL G+FQ GK+++ + + +++A + +GDAAK ++ + Sbjct: 547 PTGGLDLS------GMFQ-GKIQVASTAESEAGRKAFLAECERNPNLGDAAKMKLESCLS 599 Query: 250 NTYSMPNVSSDV 285 + S N+ D+ Sbjct: 600 DLVSTANIFKDL 611 >SB_53017| Best HMM Match : Kazal_1 (HMM E-Value=0) Length = 1488 Score = 27.5 bits (58), Expect = 9.7 Identities = 12/29 (41%), Positives = 18/29 (62%) Frame = +2 Query: 401 PRGSQFHGAYENEGNCRSLSRQNCAECSY 487 PRG Q Y G+C S+S++NC + S+ Sbjct: 1142 PRGKQI--PYGTAGDCYSVSQENCRKGSF 1168 >SB_51898| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1712 Score = 27.5 bits (58), Expect = 9.7 Identities = 11/22 (50%), Positives = 15/22 (68%) Frame = +2 Query: 455 LSRQNCAECSYHGSRVLQDSQR 520 + R N A+CS GS VLQD ++ Sbjct: 1278 IGRDNAAKCSCTGSLVLQDDEK 1299 >SB_16515| Best HMM Match : Extensin_2 (HMM E-Value=0.12) Length = 917 Score = 27.5 bits (58), Expect = 9.7 Identities = 27/77 (35%), Positives = 39/77 (50%), Gaps = 1/77 (1%) Frame = +3 Query: 243 PQQ-HIFDAKRLIGRKFEDATVQADMKHWPFEVVSGXGKPKIKVAYKGEDKTFFPEEVSS 419 PQQ H+ DA+ IGR A + DM F G P+ K K ++KT + V+S Sbjct: 840 PQQFHVSDARSRIGRLLIKAAKKNDMVTSHFT----KGGPRNK--GKEDEKTL--DRVAS 891 Query: 420 MVLTKMKETAEAYLGKT 470 M+ T++ T + GKT Sbjct: 892 MIATRLLHTNKT--GKT 906 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 21,557,014 Number of Sequences: 59808 Number of extensions: 484739 Number of successful extensions: 1432 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 1320 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1426 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1608851125 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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