BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021848 (770 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g47510.1 68418.m05866 SEC14 cytosolic factor family protein /... 31 1.1 At1g05577.1 68414.m00577 hypothetical protein 29 2.6 At5g54340.1 68418.m06767 zinc finger (C2H2 type) protein-related... 29 4.5 At4g36550.1 68417.m05190 U-box domain-containing protein low sim... 29 4.5 At3g18485.1 68416.m02349 hypothetical protein 29 4.5 At5g61780.1 68418.m07753 tudor domain-containing protein / nucle... 28 5.9 At4g17120.1 68417.m02578 expressed protein 28 7.9 >At5g47510.1 68418.m05866 SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein similar to phosphatidylinositol transfer-like protein IV (GI:14486707) [Lotus japonicus], SEC14 cytosolic factor (Phosphatidylinositol/phosphatidylcholine transfer protein) (PI/PCTP) (SP:P24859) [Kluyveromyces lactis] and to SEC14 cytosolic factor (SP:P53989) [Candida glabrata] Length = 376 Score = 30.7 bits (66), Expect = 1.1 Identities = 13/32 (40%), Positives = 21/32 (65%) Frame = +2 Query: 254 HNFLRRFLYARKHDVQQSFELLVRYHQYRREH 349 HN LRRFL R D+++S E + Y ++R ++ Sbjct: 48 HNTLRRFLKMRDFDLEKSKEAFLNYMKWRVDY 79 >At1g05577.1 68414.m00577 hypothetical protein Length = 372 Score = 29.5 bits (63), Expect = 2.6 Identities = 15/36 (41%), Positives = 18/36 (50%) Frame = -3 Query: 492 NEHA*GDQFEANTRSTQRPRLSRCANTPGNPSASAR 385 N A D +N +T +PR RC N PGNP R Sbjct: 331 NSSANNDLMTSN--NTVKPRQQRCRNIPGNPLGHQR 364 >At5g54340.1 68418.m06767 zinc finger (C2H2 type) protein-related contains Pfam domain, PF00096: Zinc finger, C2H2 type Length = 244 Score = 28.7 bits (61), Expect = 4.5 Identities = 16/34 (47%), Positives = 19/34 (55%), Gaps = 1/34 (2%) Frame = -3 Query: 468 FEANTRSTQRPRLSRCANTPGNPSAS-ARNTPPS 370 F+ N RPR+SR GNP S +RN PPS Sbjct: 184 FDGNLIIPVRPRVSRYHFVAGNPLDSISRNIPPS 217 >At4g36550.1 68417.m05190 U-box domain-containing protein low similarity to immediate-early fungal elicitor protein CMPG1 [Petroselinum crispum] GI:14582200; contains Pfam profile PF04564: U-box domain Length = 577 Score = 28.7 bits (61), Expect = 4.5 Identities = 14/27 (51%), Positives = 17/27 (62%) Frame = +3 Query: 501 SLDSASYH*THTGGYPEPSQRIRNYSG 581 S+DS+SY GGY P QRI + SG Sbjct: 198 SIDSSSYSKMSKGGYFTPMQRIDSASG 224 >At3g18485.1 68416.m02349 hypothetical protein Length = 386 Score = 28.7 bits (61), Expect = 4.5 Identities = 13/35 (37%), Positives = 16/35 (45%) Frame = -1 Query: 446 HNARVYPVVPTHPATRRRALVTRPRLPKPTARHVP 342 HN +VYP +P P RR R L +VP Sbjct: 138 HNGKVYPRIPAQPGPARRDAFMRDLLESHFLPNVP 172 >At5g61780.1 68418.m07753 tudor domain-containing protein / nuclease family protein contains Pfam domains PF00567: Tudor domain and PF00565: Staphylococcal nuclease homologue Length = 985 Score = 28.3 bits (60), Expect = 5.9 Identities = 14/41 (34%), Positives = 18/41 (43%) Frame = -3 Query: 471 QFEANTRSTQRPRLSRCANTPGNPSASARNTPPSAEAHSSS 349 + E Q+ R + PG AS RN PPSA S + Sbjct: 135 ELEQLEEQAQQEGFGRWSKVPGAAEASIRNLPPSAVGDSGN 175 >At4g17120.1 68417.m02578 expressed protein Length = 1661 Score = 27.9 bits (59), Expect = 7.9 Identities = 14/48 (29%), Positives = 24/48 (50%) Frame = +1 Query: 529 RTLADIQNQANGYVIIVDWTEFTFKQSCSLQAKVLKMMIDCLQDCMPV 672 RT + N + + +VDW E F + SL VL++++ + PV Sbjct: 594 RTCGSVSNMLSSELEVVDWNEIFFFRIDSLDDFVLELIVTDVGKGAPV 641 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,766,485 Number of Sequences: 28952 Number of extensions: 363959 Number of successful extensions: 1106 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 1058 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1106 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1716774400 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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