BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021845 (693 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 36 0.007 >SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 538 Score = 35.5 bits (78), Expect = 0.007 Identities = 15/28 (53%), Positives = 22/28 (78%) Frame = +3 Query: 186 DLNDQITKQGDLVRSLKAAKAEKQKLTK 269 D+N++IT QG+LVRSLK+ A K+ + K Sbjct: 7 DINERITSQGNLVRSLKSQGASKEDIDK 34 Score = 32.3 bits (70), Expect = 0.068 Identities = 14/26 (53%), Positives = 20/26 (76%) Frame = +2 Query: 392 LDQQITKQGDLVRSLKTSKAEKSKID 469 ++++IT QG+LVRSLK+ A K ID Sbjct: 8 INERITSQGNLVRSLKSQGASKEDID 33 Score = 31.5 bits (68), Expect = 0.12 Identities = 14/29 (48%), Positives = 21/29 (72%) Frame = +1 Query: 592 LDRDVTAQGDLVRSLKASKADKAKIDEAV 678 ++ +T+QG+LVRSLK+ A K ID+ V Sbjct: 8 INERITSQGNLVRSLKSQGASKEDIDKEV 36 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,167,943 Number of Sequences: 5004 Number of extensions: 33324 Number of successful extensions: 104 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 98 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 104 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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