BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021835 (831 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces pomb... 90 4e-19 SPAC144.09c |sfc2||RNA polymerase III transcription factor TFIII... 28 1.4 SPAC24B11.07c |||ketopantoate reductase |Schizosaccharomyces pom... 28 1.9 SPBC8D2.07c |sfc9||transcription factor TFIIIC complex subunit S... 27 4.3 SPCC1840.05c |||phosphomannomutase |Schizosaccharomyces pombe|ch... 26 5.7 SPBC4F6.11c |||asparagine synthase |Schizosaccharomyces pombe|ch... 26 5.7 SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr 2|||... 26 7.5 SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|... 25 10.0 >SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces pombe|chr 2|||Manual Length = 557 Score = 89.8 bits (213), Expect = 4e-19 Identities = 44/88 (50%), Positives = 63/88 (71%) Frame = -2 Query: 761 SYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGADELAQGYIYFRDAPSEKDAHKESVR 582 +YD+TTIRAS PMYLLS+ IK + +V SGEG+DE+ GY+YF +APS + H E VR Sbjct: 325 TYDVTTIRASTPMYLLSRKIKAQ-GVKMVLSGEGSDEIFGGYLYFGNAPSREAFHSECVR 383 Query: 581 LLSDIYLYDGLRADRTTSAFSLELQYRF 498 + +++L D LRA+++T A+ LE + F Sbjct: 384 RVKNLHLSDCLRANKSTMAWGLEARVPF 411 Score = 46.8 bits (106), Expect = 4e-06 Identities = 34/91 (37%), Positives = 46/91 (50%), Gaps = 7/91 (7%) Frame = -1 Query: 507 VPFLDIQFTHHYLSIPPKLRQPQNG-VEKHLLRSSFAK---SGL---LPDCVLWRHKEAF 349 VPFLD F L+I P+ + NG EK++LR +F S L LP +LWR KE F Sbjct: 409 VPFLDKDFLEVALNIDPEEKMYINGRKEKYILRKAFDTTHDSSLQPYLPQDILWRQKEQF 468 Query: 348 SDGVSSVKKSLFSVIKEITTERLQEDDIQYP 256 SDGV S +K+ + +D+ P Sbjct: 469 SDGVG---YSWIDALKDTAELCISDDEFALP 496 Score = 37.1 bits (82), Expect = 0.003 Identities = 21/51 (41%), Positives = 28/51 (54%), Gaps = 3/51 (5%) Frame = -3 Query: 247 PKTNESKYYRYVFEKSFPGQHNFTPYYWMPK--WVQVSDPSARF-IKHYAA 104 P T E+ +YR +F++ FP Q T W+PK W DPS R+ H AA Sbjct: 505 PTTKEAFWYRKLFDEIFPRQCADTVMRWVPKAEWGCPEDPSGRYQAGHVAA 555 >SPAC144.09c |sfc2||RNA polymerase III transcription factor TFIIIA|Schizosaccharomyces pombe|chr 1|||Manual Length = 374 Score = 28.3 bits (60), Expect = 1.4 Identities = 10/38 (26%), Positives = 17/38 (44%) Frame = -1 Query: 261 YPDCSRKRMNPNITATYLRNHFQDNIILLRTIGCQNGF 148 Y +C +K P++ +LR H + + GC F Sbjct: 27 YEECGKKYSRPSLLEQHLRTHSNERPFVCDYTGCSKAF 64 >SPAC24B11.07c |||ketopantoate reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 561 Score = 27.9 bits (59), Expect = 1.9 Identities = 12/33 (36%), Positives = 19/33 (57%) Frame = -3 Query: 277 RRRYTISRLQPKTNESKYYRYVFEKSFPGQHNF 179 R Y +S+L+ T++S+ + YVF P NF Sbjct: 66 RPTYRLSKLEEVTSDSEPFDYVFVSIKPNSRNF 98 >SPBC8D2.07c |sfc9||transcription factor TFIIIC complex subunit Sfc9 |Schizosaccharomyces pombe|chr 2|||Manual Length = 673 Score = 26.6 bits (56), Expect = 4.3 Identities = 20/75 (26%), Positives = 34/75 (45%) Frame = -2 Query: 731 LPMYLLSKYIKEKTDTTVVFSGEGADELAQGYIYFRDAPSEKDAHKESVRLLSDIYLYDG 552 L + L++ +KEKTD + + + L Y +F + H + LS++YL Sbjct: 444 LEISLMNDTLKEKTDIFTMLTNSLSSFLVTDYDFFLEL-----KHLIDISSLSNLYLDSS 498 Query: 551 LRADRTTSAFSLELQ 507 L A R +S+ Q Sbjct: 499 LNAMRLLYGWSVYKQ 513 >SPCC1840.05c |||phosphomannomutase |Schizosaccharomyces pombe|chr 3|||Manual Length = 587 Score = 26.2 bits (55), Expect = 5.7 Identities = 13/31 (41%), Positives = 20/31 (64%), Gaps = 3/31 (9%) Frame = +2 Query: 302 FMTENNDFLTEDTPSLNA---SLCRHSTQSG 385 + T+N+ FL+ DTP L A +L + T+SG Sbjct: 455 YTTQNSYFLSRDTPKLRALVDALRHYDTKSG 485 >SPBC4F6.11c |||asparagine synthase |Schizosaccharomyces pombe|chr 2|||Manual Length = 548 Score = 26.2 bits (55), Expect = 5.7 Identities = 26/87 (29%), Positives = 39/87 (44%), Gaps = 3/87 (3%) Frame = -2 Query: 680 VVFSGEGADELAQGYIYFRDAPSEKD--AHKESVRLLSDIYLYDGL-RADRTTSAFSLEL 510 V+ SG GADE GY+ A K +E ++L D + L R DR S E+ Sbjct: 409 VLISGLGADEQLGGYMRHLRAFERKGMAGLEEELQLDIDRIPHRNLGRDDRVMSNQGKEV 468 Query: 509 QYRF*ISNSLITIFQYLLNYDNPKTAL 429 +Y F + L+ +F + D + L Sbjct: 469 RYPF-LDERLMALFSKMKTTDKMRLDL 494 >SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr 2|||Manual Length = 404 Score = 25.8 bits (54), Expect = 7.5 Identities = 11/23 (47%), Positives = 12/23 (52%) Frame = -3 Query: 223 YRYVFEKSFPGQHNFTPYYWMPK 155 YR FE+ F G N Y W PK Sbjct: 349 YRPDFEQCFTGDMNGNIYMWSPK 371 >SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr 1|||Manual Length = 1234 Score = 25.4 bits (53), Expect = 10.0 Identities = 15/36 (41%), Positives = 21/36 (58%) Frame = -2 Query: 530 SAFSLELQYRF*ISNSLITIFQYLLNYDNPKTALRN 423 S +SL LQ+R S L I + L YD+P +LR+ Sbjct: 146 SLWSLSLQHRQLSSLILRGIMEDLYQYDDPVASLRS 181 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,227,670 Number of Sequences: 5004 Number of extensions: 68502 Number of successful extensions: 194 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 181 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 193 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 408446760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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