Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021835
(831 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g47340.3 68416.m05147 asparagine synthetase 1 [glutamine-hydr... 94 9e-20
At3g47340.2 68416.m05146 asparagine synthetase 1 [glutamine-hydr... 94 9e-20
At3g47340.1 68416.m05145 asparagine synthetase 1 [glutamine-hydr... 94 9e-20
At5g10240.1 68418.m01189 asparagine synthetase 3 (ASN3) identica... 90 2e-18
At5g65010.2 68418.m08178 asparagine synthetase 2 (ASN2) identica... 89 4e-18
At5g65010.1 68418.m08177 asparagine synthetase 2 (ASN2) identica... 89 4e-18
At4g25420.1 68417.m03656 gibberellin 20-oxidase identical to GI:... 30 1.6
At4g05523.1 68417.m00840 hypothetical protein 29 2.9
At3g01320.1 68416.m00045 paired amphipathic helix repeat-contain... 29 3.8
At4g37880.1 68417.m05357 expressed protein 29 5.0
At2g30720.1 68415.m03747 thioesterase family protein similar to ... 28 6.6
At2g20810.1 68415.m02448 glycosyl transferase family 8 protein c... 28 6.6
At5g48370.1 68418.m05976 thioesterase family protein similar to ... 28 8.7
>At3g47340.3 68416.m05147 asparagine synthetase 1
[glutamine-hydrolyzing] / glutamine-dependent asparagine
synthetase 1 (ASN1) identical to SP|P49078 Asparagine
synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)
(Glutamine- dependent asparagine synthetase)
{Arabidopsis thaliana}
Length = 456
Score = 94.3 bits (224), Expect = 9e-20
Identities = 44/88 (50%), Positives = 64/88 (72%)
Frame = -2
Query: 761 SYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGADELAQGYIYFRDAPSEKDAHKESVR 582
+YD+TTIRAS PM+L+S+ IK +V SGEGADE+ GY+YF AP++K+ H+E+ R
Sbjct: 312 TYDVTTIRASTPMFLMSRKIKS-LGVKMVLSGEGADEIFGGYLYFHKAPNKKEFHQETCR 370
Query: 581 LLSDIYLYDGLRADRTTSAFSLELQYRF 498
+ ++ YD LRA+++TSAF LE + F
Sbjct: 371 KIKALHKYDCLRANKSTSAFGLEARVPF 398
>At3g47340.2 68416.m05146 asparagine synthetase 1
[glutamine-hydrolyzing] / glutamine-dependent asparagine
synthetase 1 (ASN1) identical to SP|P49078 Asparagine
synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)
(Glutamine- dependent asparagine synthetase)
{Arabidopsis thaliana}
Length = 512
Score = 94.3 bits (224), Expect = 9e-20
Identities = 44/88 (50%), Positives = 64/88 (72%)
Frame = -2
Query: 761 SYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGADELAQGYIYFRDAPSEKDAHKESVR 582
+YD+TTIRAS PM+L+S+ IK +V SGEGADE+ GY+YF AP++K+ H+E+ R
Sbjct: 312 TYDVTTIRASTPMFLMSRKIKS-LGVKMVLSGEGADEIFGGYLYFHKAPNKKEFHQETCR 370
Query: 581 LLSDIYLYDGLRADRTTSAFSLELQYRF 498
+ ++ YD LRA+++TSAF LE + F
Sbjct: 371 KIKALHKYDCLRANKSTSAFGLEARVPF 398
Score = 28.7 bits (61), Expect = 5.0
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -3
Query: 247 PKTNESKYYRYVFEKSFP 194
P T E+ YYR +FE+ FP
Sbjct: 490 PNTKEAYYYRMIFERFFP 507
>At3g47340.1 68416.m05145 asparagine synthetase 1
[glutamine-hydrolyzing] / glutamine-dependent asparagine
synthetase 1 (ASN1) identical to SP|P49078 Asparagine
synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)
(Glutamine- dependent asparagine synthetase)
{Arabidopsis thaliana}
Length = 584
Score = 94.3 bits (224), Expect = 9e-20
Identities = 44/88 (50%), Positives = 64/88 (72%)
Frame = -2
Query: 761 SYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGADELAQGYIYFRDAPSEKDAHKESVR 582
+YD+TTIRAS PM+L+S+ IK +V SGEGADE+ GY+YF AP++K+ H+E+ R
Sbjct: 312 TYDVTTIRASTPMFLMSRKIKS-LGVKMVLSGEGADEIFGGYLYFHKAPNKKEFHQETCR 370
Query: 581 LLSDIYLYDGLRADRTTSAFSLELQYRF 498
+ ++ YD LRA+++TSAF LE + F
Sbjct: 371 KIKALHKYDCLRANKSTSAFGLEARVPF 398
Score = 28.7 bits (61), Expect = 5.0
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -3
Query: 247 PKTNESKYYRYVFEKSFP 194
P T E+ YYR +FE+ FP
Sbjct: 490 PNTKEAYYYRMIFERFFP 507
>At5g10240.1 68418.m01189 asparagine synthetase 3 (ASN3) identical
to asparagine synthetase (ASN3) [Arabidopsis thaliana]
GI:3859534
Length = 578
Score = 89.8 bits (213), Expect = 2e-18
Identities = 41/88 (46%), Positives = 64/88 (72%)
Frame = -2
Query: 761 SYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGADELAQGYIYFRDAPSEKDAHKESVR 582
+YD+TTIRAS PM+L+S+ IK +V SGEG+DE+ GY+YF AP++K+ H+E+ R
Sbjct: 312 TYDVTTIRASTPMFLMSRKIKS-LGVKMVLSGEGSDEIFGGYLYFHKAPNKKEFHEETCR 370
Query: 581 LLSDIYLYDGLRADRTTSAFSLELQYRF 498
+ ++ YD LRA+++TSA+ +E + F
Sbjct: 371 KIKALHQYDCLRANKSTSAWGVEARVPF 398
Score = 28.7 bits (61), Expect = 5.0
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -3
Query: 247 PKTNESKYYRYVFEKSFP 194
P T E+ YYR +FEK FP
Sbjct: 490 PLTKEAYYYRTIFEKFFP 507
>At5g65010.2 68418.m08178 asparagine synthetase 2 (ASN2) identical
to asparagine synthetase (ASN2) [Arabidopsis thaliana]
GI:3859536
Length = 579
Score = 88.6 bits (210), Expect = 4e-18
Identities = 40/88 (45%), Positives = 64/88 (72%)
Frame = -2
Query: 761 SYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGADELAQGYIYFRDAPSEKDAHKESVR 582
+YD+TTIRAS PM+L+S+ IK +V SGEG+DE+ GY+YF AP++K+ H+E+ R
Sbjct: 312 TYDVTTIRASTPMFLMSRKIKS-LGVKMVLSGEGSDEILGGYLYFHKAPNKKEFHEETCR 370
Query: 581 LLSDIYLYDGLRADRTTSAFSLELQYRF 498
+ ++ +D LRA+++TSA+ +E + F
Sbjct: 371 KIKALHQFDCLRANKSTSAWGVEARVPF 398
Score = 29.9 bits (64), Expect = 2.2
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = -3
Query: 247 PKTNESKYYRYVFEKSFPGQ 188
P T E+ YYR +FEK FP Q
Sbjct: 490 PLTKEAYYYRTIFEKFFPKQ 509
>At5g65010.1 68418.m08177 asparagine synthetase 2 (ASN2) identical
to asparagine synthetase (ASN2) [Arabidopsis thaliana]
GI:3859536
Length = 578
Score = 88.6 bits (210), Expect = 4e-18
Identities = 40/88 (45%), Positives = 64/88 (72%)
Frame = -2
Query: 761 SYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGADELAQGYIYFRDAPSEKDAHKESVR 582
+YD+TTIRAS PM+L+S+ IK +V SGEG+DE+ GY+YF AP++K+ H+E+ R
Sbjct: 312 TYDVTTIRASTPMFLMSRKIKS-LGVKMVLSGEGSDEILGGYLYFHKAPNKKEFHEETCR 370
Query: 581 LLSDIYLYDGLRADRTTSAFSLELQYRF 498
+ ++ +D LRA+++TSA+ +E + F
Sbjct: 371 KIKALHQFDCLRANKSTSAWGVEARVPF 398
Score = 28.7 bits (61), Expect = 5.0
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -3
Query: 247 PKTNESKYYRYVFEKSFP 194
P T E+ YYR +FEK FP
Sbjct: 490 PLTKEAYYYRTIFEKFFP 507
>At4g25420.1 68417.m03656 gibberellin 20-oxidase identical to
GI:1109695
Length = 377
Score = 30.3 bits (65), Expect = 1.6
Identities = 17/44 (38%), Positives = 23/44 (52%)
Frame = -2
Query: 230 QILPLRI*EIISRTT*FYSVLLDAKMGSGFRPFGKVY*TLCGKM 99
+ L R + +SR+ DA +G GF+PFGKVY C M
Sbjct: 152 ETLSFRFCDDMSRSKSVQDYFCDA-LGHGFQPFGKVYQEYCEAM 194
>At4g05523.1 68417.m00840 hypothetical protein
Length = 221
Score = 29.5 bits (63), Expect = 2.9
Identities = 18/46 (39%), Positives = 25/46 (54%)
Frame = -2
Query: 611 EKDAHKESVRLLSDIYLYDGLRADRTTSAFSLELQYRF*ISNSLIT 474
EK+ E R LS I L + R +R SL+LQY+ +S +L T
Sbjct: 140 EKEIRNEIERALSAIALVEKYRENRREVERSLDLQYKREVSEALET 185
>At3g01320.1 68416.m00045 paired amphipathic helix repeat-containing
protein low similarity to transcription co-repressor
Sin3 [Xenopus laevis] GI:4960210; contains Pfam profile
PF02671: Paired amphipathic helix repeat
Length = 1378
Score = 29.1 bits (62), Expect = 3.8
Identities = 15/58 (25%), Positives = 29/58 (50%), Gaps = 2/58 (3%)
Frame = -1
Query: 324 KSLFSVIKEITTERLQEDDIQYPDCS--RKRMNPNITATYLRNHFQDNIILLRTIGCQ 157
KSL + IKE+ + +DD+ + R+ +NPN+ YL +++ + C+
Sbjct: 666 KSLLAEIKELKEKSQNDDDVLLSISAGYRQPINPNLEYEYLNRAIHEDMFKVVQFSCE 723
>At4g37880.1 68417.m05357 expressed protein
Length = 388
Score = 28.7 bits (61), Expect = 5.0
Identities = 19/69 (27%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
Frame = -1
Query: 381 DCVLWRHKEAFSDGVSSVKKSLFSVIK-EITTERLQEDDIQYPDCSRKRMNPNITATYLR 205
D +L H+ +D V V + + + E T + L +YP K++NP+I+ Y
Sbjct: 49 DTMLLDHRSILAD-VKKVFMEIAPITQLEATEKELHAALTKYPKVLEKQLNPDISKAYRH 107
Query: 204 N-HFQDNII 181
N F +I+
Sbjct: 108 NVEFDTHIV 116
>At2g30720.1 68415.m03747 thioesterase family protein similar to
SP|Q9R0X4 48 kDa acyl-CoA thioester hydrolase,
mitochondrial precursor (EC 3.1.2.-) {Mus musculus};
contains Pfam profile PF03061: thioesterase family
protein
Length = 455
Score = 28.3 bits (60), Expect = 6.6
Identities = 11/54 (20%), Positives = 25/54 (46%)
Frame = +2
Query: 305 MTENNDFLTEDTPSLNASLCRHSTQSGNNPLFAKLLLNRCFSTPFWGCRSLGGI 466
+ + N L +DT N+ +C+ ++ + +F L+ + F F + G+
Sbjct: 295 LADRNSILIKDTSHENSLICQPQQRNIHGRIFGGFLMRKAFELAFSNAYTFAGV 348
>At2g20810.1 68415.m02448 glycosyl transferase family 8 protein
contains Pfam profile: PF01501 glycosyl transferase
family 8
Length = 536
Score = 28.3 bits (60), Expect = 6.6
Identities = 19/52 (36%), Positives = 27/52 (51%), Gaps = 2/52 (3%)
Frame = -1
Query: 330 VKKSLFSVIKEITTERLQEDDIQYPDCSRKRMNPNITATYLRNH--FQDNII 181
V KSL+ + +TTE Q D+Q R R++ +T L + F DNII
Sbjct: 188 VPKSLYCLGVRLTTEWFQNLDLQRTLKERSRVDSKLTDNSLYHFCVFSDNII 239
>At5g48370.1 68418.m05976 thioesterase family protein similar to
SP|Q9R0X4 48 kDa acyl-CoA thioester hydrolase,
mitochondrial precursor (EC 3.1.2.-) {Mus musculus};
contains Pfam profile PF03061: thioesterase family
protein
Length = 438
Score = 27.9 bits (59), Expect = 8.7
Identities = 12/54 (22%), Positives = 26/54 (48%)
Frame = +2
Query: 305 MTENNDFLTEDTPSLNASLCRHSTQSGNNPLFAKLLLNRCFSTPFWGCRSLGGI 466
+ + N L +DT N+ +C+ ++ + +F L++R F F + G+
Sbjct: 267 LADRNSILLKDTRLENSLICQPQQRNIHGRIFGGFLMHRAFELAFSTAYTFAGL 320
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,231,235
Number of Sequences: 28952
Number of extensions: 338119
Number of successful extensions: 846
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 807
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 840
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1911862400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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