BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021834 (828 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 282 5e-77 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 180 3e-46 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 177 2e-45 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 159 6e-40 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.2 SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 27 4.3 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.3 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 5.7 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 7.5 SPBC21C3.20c |git1||C2 domain protein Git1|Schizosaccharomyces p... 26 7.5 SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi... 25 9.9 SPCC1020.03 |||mitochondrial iron ion transporter|Schizosaccharo... 25 9.9 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 9.9 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 282 bits (691), Expect = 5e-77 Identities = 133/192 (69%), Positives = 153/192 (79%) Frame = +1 Query: 253 QYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVL 432 +YVPRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VL Sbjct: 58 KYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVL 117 Query: 433 DVVRKEAESCDCLQGFQLTHSLGGGTVPVWAPXXISKIREEYPDRIMNTYSVVPSPKVSD 612 DVVR+EAE+CD LQGFQLTHSLGGGT +SKIREEYPDR+M T+SV P+PK SD Sbjct: 118 DVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSD 177 Query: 613 TVVEPYNATLSVHQLVENTDETYCIDNEALYDICFPHAKTIHTHIRRPEPLVSLTMSGVT 792 TVVEPYNATLS+HQLVEN+DET+CIDNEAL I K LVS M+GVT Sbjct: 178 TVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVT 237 Query: 793 TCLRFPGQLNAD 828 T RFPG+LN+D Sbjct: 238 TSFRFPGELNSD 249 Score = 99 bits (238), Expect = 4e-22 Identities = 41/57 (71%), Positives = 48/57 (84%) Frame = +2 Query: 83 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGG 253 MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GG Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGG 57 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 180 bits (437), Expect = 3e-46 Identities = 82/192 (42%), Positives = 121/192 (63%) Frame = +1 Query: 253 QYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVL 432 ++VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL Sbjct: 64 KFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVL 123 Query: 433 DVVRKEAESCDCLQGFQLTHSLGGGTVPVWAPXXISKIREEYPDRIMNTYSVVPSPKVSD 612 + +R+ A++C LQGF + HS GGGT + ++ EY + +SV P+P+VS Sbjct: 124 ERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVST 183 Query: 613 TVVEPYNATLSVHQLVENTDETYCIDNEALYDICFPHAKTIHTHIRRPEPLVSLTMSGVT 792 +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC + L++ +S +T Sbjct: 184 SVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSIT 243 Query: 793 TCLRFPGQLNAD 828 LRF G LN D Sbjct: 244 ASLRFAGSLNVD 255 Score = 44.4 bits (100), Expect = 2e-05 Identities = 22/56 (39%), Positives = 31/56 (55%) Frame = +2 Query: 83 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASG 250 MRE++ + GQ G QIG WE+ EHGI P G +S ++ + N Y N+ G Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENS--EVHKNNSYLNDGFG 54 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 177 bits (430), Expect = 2e-45 Identities = 81/192 (42%), Positives = 118/192 (61%) Frame = +1 Query: 253 QYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVL 432 +YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V Sbjct: 60 KYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVT 119 Query: 433 DVVRKEAESCDCLQGFQLTHSLGGGTVPVWAPXXISKIREEYPDRIMNTYSVVPSPKVSD 612 D +R+ A++C LQGF + HS GGGT + + ++ EY + +SV P+P+VS Sbjct: 120 DKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVST 179 Query: 613 TVVEPYNATLSVHQLVENTDETYCIDNEALYDICFPHAKTIHTHIRRPEPLVSLTMSGVT 792 +VVEPYN+ L+ H ++ D T+ +DNE+ YDIC + L++ +S +T Sbjct: 180 SVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSIT 239 Query: 793 TCLRFPGQLNAD 828 LRF G LN D Sbjct: 240 ASLRFEGSLNVD 251 Score = 44.4 bits (100), Expect = 2e-05 Identities = 19/34 (55%), Positives = 21/34 (61%) Frame = +2 Query: 83 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG 184 MREI+ I GQ G QIG WE+ EHGI P G Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNG 34 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 159 bits (385), Expect = 6e-40 Identities = 76/195 (38%), Positives = 117/195 (60%), Gaps = 3/195 (1%) Frame = +1 Query: 253 QYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSG--AGNNWAKGHYTEGAELVDS 426 +Y+PRAIL+DLEP ++++ S +G ++ P+N + ++G AGNNWA G Y+ + + Sbjct: 59 RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFED 117 Query: 427 VLDVVRKEAESCDCLQGFQLTHSLGGGTVPVWAPXXISKIREEYPDRIMNTYSVVP-SPK 603 ++D++ +EA+ D L+GF L HS+ GGT + ++ + YP +I+ TYSV P S Sbjct: 118 IMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQS 177 Query: 604 VSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFPHAKTIHTHIRRPEPLVSLTMS 783 VSD VV+PYN+ L++ +L N D +DN AL I T + + LVS MS Sbjct: 178 VSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMS 237 Query: 784 GVTTCLRFPGQLNAD 828 TT LR+PG +N D Sbjct: 238 ASTTTLRYPGYMNND 252 Score = 56.4 bits (130), Expect = 5e-09 Identities = 23/53 (43%), Positives = 37/53 (69%) Frame = +2 Query: 86 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEA 244 REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQS 55 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 3.2 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +2 Query: 122 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 223 N++G E+++++ +DPT A + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr 2|||Manual Length = 807 Score = 26.6 bits (56), Expect = 4.3 Identities = 22/73 (30%), Positives = 34/73 (46%), Gaps = 4/73 (5%) Frame = -2 Query: 527 GAHTGTVPPPSECVSWNPWR-QSH---DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDC 360 G T+ P + +N R ++H ++A+ RTTS T+ +PS L P P Sbjct: 363 GEKMDTIHYPDDLSYFNSSRSKAHVNKNAAADRTTSPTQGQPESPS---KSILLRPPPSI 419 Query: 359 PKTKLSGRKICPK 321 + S R+ CPK Sbjct: 420 ASSPESKRRKCPK 432 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 4.3 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +1 Query: 316 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 417 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 26.2 bits (55), Expect = 5.7 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = +1 Query: 385 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 474 A+GH G ELV + D +RK++E+ L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 25.8 bits (54), Expect = 7.5 Identities = 12/44 (27%), Positives = 20/44 (45%) Frame = +1 Query: 337 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 468 RP +F G++ G + E D ++ + + ESCDC Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997 >SPBC21C3.20c |git1||C2 domain protein Git1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1098 Score = 25.8 bits (54), Expect = 7.5 Identities = 14/58 (24%), Positives = 30/58 (51%) Frame = +1 Query: 556 YPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFPHAK 729 + D + + +V+ S K++ +V P A S L+++ + +C+ +YD P+ K Sbjct: 782 FKDMEVPSATVIISIKMAHLLVTP-KAPYSFSVLLQDPESEFCVHTRDVYDELVPNWK 838 >SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schizosaccharomyces pombe|chr 2|||Manual Length = 766 Score = 25.4 bits (53), Expect = 9.9 Identities = 15/46 (32%), Positives = 27/46 (58%) Frame = -3 Query: 235 VVYIDALQLQVRVPMVSTGGVDAVLVGDDLPELSSDLVAALTSLDM 98 ++Y+DA L +++ + G A V DLP L +D V++L S+ + Sbjct: 511 ILYVDAKTLFIQLLRLLPSGHPATRVPLDLP-LIADSVSSLKSMSL 555 >SPCC1020.03 |||mitochondrial iron ion transporter|Schizosaccharomyces pombe|chr 3|||Manual Length = 397 Score = 25.4 bits (53), Expect = 9.9 Identities = 10/16 (62%), Positives = 12/16 (75%) Frame = +1 Query: 700 LYDICFPHAKTIHTHI 747 LY I FP+A + HTHI Sbjct: 169 LYTILFPYAGSEHTHI 184 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 25.4 bits (53), Expect = 9.9 Identities = 13/32 (40%), Positives = 16/32 (50%) Frame = -2 Query: 374 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 279 P+ PK L R I P GPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,377,684 Number of Sequences: 5004 Number of extensions: 68622 Number of successful extensions: 210 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 195 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 203 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 406444570 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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