Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021820
(752 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch... 140 2e-34
SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 118 1e-27
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 43 5e-05
SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc... 42 7e-05
SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomy... 29 0.71
SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch... 29 0.94
SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c... 29 0.94
SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces... 27 2.2
SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces ... 27 3.8
SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase Pef1|... 26 6.6
SPCC16A11.13 |usp106|luc7|U1 snRNP-associated protein Usp106|Sch... 26 6.6
SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pom... 26 6.6
SPBC2D10.07c |||mitochondrial inner membrane peptidase complex c... 25 8.8
>SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 778
Score = 140 bits (339), Expect = 2e-34
Identities = 65/85 (76%), Positives = 70/85 (82%)
Frame = +2
Query: 257 HQIILENYAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVK 436
HQI+LENYAFPG L+IGTDSHTPN ADAVDVMAN+PWELKCPKVIGVK
Sbjct: 170 HQIVLENYAFPGGLLIGTDSHTPNAGGLGMVAIGVGGADAVDVMANLPWELKCPKVIGVK 229
Query: 437 LTGKLTGWTSPKDVILKVAGILTVR 511
LTG+L GWTSPKDVILKVAGILTV+
Sbjct: 230 LTGQLKGWTSPKDVILKVAGILTVK 254
Score = 133 bits (322), Expect = 2e-32
Identities = 61/84 (72%), Positives = 70/84 (83%)
Frame = +3
Query: 3 LRLRPDRVAMQDATAQMAMLQFISSGLPRVAVPSTIHCDHLIEAQVGGEKDLARAKDLNK 182
L+LRPDRVA QDATAQMA+LQF+S+G+P VAVP T+HCDHLIEA GG DL RA NK
Sbjct: 85 LKLRPDRVACQDATAQMAILQFMSAGMPEVAVPVTVHCDHLIEAYEGGPIDLERANVTNK 144
Query: 183 EVYKFLETAGAKYGVGFWKPGSGI 254
EVY FL+TA AKY +GFW+PGSGI
Sbjct: 145 EVYDFLQTACAKYNIGFWRPGSGI 168
Score = 113 bits (271), Expect = 3e-26
Identities = 50/81 (61%), Positives = 61/81 (75%)
Frame = +1
Query: 508 EGGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLKSTGRHDIAA 687
+GGTGAIVEY GPGV+ +SCTGM TICNMGAEIGATTS+FP+N RM YL++T R IA
Sbjct: 254 KGGTGAIVEYFGPGVESLSCTGMGTICNMGAEIGATTSIFPFNPRMSEYLRATNRSAIAD 313
Query: 688 TANSYKHLLTPDNKAPYDQLI 750
A + ++ D A YDQ+I
Sbjct: 314 YAEEFAPIIAADENAHYDQII 334
>SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 905
Score = 118 bits (283), Expect = 1e-27
Identities = 53/85 (62%), Positives = 62/85 (72%)
Frame = +2
Query: 257 HQIILENYAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVK 436
HQI+LENYA PG +M+GTDSHTPN ADAVD M N PWELK PK+IGV
Sbjct: 174 HQIVLENYAAPGGMMLGTDSHTPNAGGLGMIAIGVGGADAVDAMTNTPWELKAPKIIGVN 233
Query: 437 LTGKLTGWTSPKDVILKVAGILTVR 511
LTG ++GWT+PKD+IL +AG LTVR
Sbjct: 234 LTGAMSGWTTPKDLILHLAGKLTVR 258
Score = 106 bits (255), Expect = 3e-24
Identities = 46/84 (54%), Positives = 62/84 (73%)
Frame = +3
Query: 3 LRLRPDRVAMQDATAQMAMLQFISSGLPRVAVPSTIHCDHLIEAQVGGEKDLARAKDLNK 182
L+L PDRVAMQDA+AQMA+LQF++ GL + +P++IHCDHLI G D+ + NK
Sbjct: 89 LKLNPDRVAMQDASAQMALLQFMTCGLEKTMIPASIHCDHLIVGHRGANSDIPDSIANNK 148
Query: 183 EVYKFLETAGAKYGVGFWKPGSGI 254
E++ FL++A KYG+ FW PGSGI
Sbjct: 149 EIFDFLQSAAKKYGIQFWGPGSGI 172
Score = 99.1 bits (236), Expect = 6e-22
Identities = 47/83 (56%), Positives = 58/83 (69%), Gaps = 3/83 (3%)
Frame = +1
Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLKSTGRHDIAAT 690
GGTG I+EY GPGV +SCTGMAT+CNMGAE+GATTS+FPY M YL +T R ++A
Sbjct: 259 GGTGHIIEYFGPGVASLSCTGMATVCNMGAEVGATTSIFPYTDSMRRYLIATHRAEVADA 318
Query: 691 A---NSYKHLLTPDNKAPYDQLI 750
A +S + L D A YDQ+I
Sbjct: 319 ASEVHSEYNYLAADTGAKYDQII 341
>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 721
Score = 42.7 bits (96), Expect = 5e-05
Identities = 26/88 (29%), Positives = 39/88 (44%), Gaps = 1/88 (1%)
Frame = +2
Query: 257 HQIILEN-YAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGV 433
HQI++E YA PG + + +DSH+ DA + A + P + V
Sbjct: 163 HQIMVEQGYAMPGSMAVASDSHSNTYGGVGCLGTPIVRTDAAAIWATGQTWWQIPPIARV 222
Query: 434 KLTGKLTGWTSPKDVILKVAGILTVREV 517
L G+L S KD+I+ + G EV
Sbjct: 223 NLVGQLPKGLSGKDIIVSLCGAFNHDEV 250
>SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 758
Score = 42.3 bits (95), Expect = 7e-05
Identities = 23/80 (28%), Positives = 39/80 (48%), Gaps = 3/80 (3%)
Frame = +1
Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLKS---TGRHDI 681
GGTG+++E+ G ++ +S ++CNM E GA + ++ Y+K+ + D
Sbjct: 207 GGTGSVIEFCGEAIEGLSMEARMSMCNMSIEAGARAGMIAPDATTFEYVKNRPLAPKGDD 266
Query: 682 AATANSYKHLLTPDNKAPYD 741
A +Y L D A YD
Sbjct: 267 WEQAVAYWKTLRSDENAKYD 286
Score = 31.9 bits (69), Expect = 0.10
Identities = 17/77 (22%), Positives = 34/77 (44%)
Frame = +2
Query: 272 ENYAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKL 451
+ + PG ++ DSHT ++ V+A + K + +++ GKL
Sbjct: 127 QGFTLPGTTLVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTILQRKSKNMRIRVNGKL 186
Query: 452 TGWTSPKDVILKVAGIL 502
+ KD+IL + G++
Sbjct: 187 PEGIASKDLILHIIGVI 203
>SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 421
Score = 29.1 bits (62), Expect = 0.71
Identities = 16/48 (33%), Positives = 26/48 (54%), Gaps = 3/48 (6%)
Frame = -1
Query: 236 PETDSIFSSGSL*KLVYFFVEVLSPGQILLASDLGFDQV---ITMNGG 102
P+T+ + ++G+ F L+PG ++ + FDQ ITMNGG
Sbjct: 93 PDTEIVVTAGANEGFFSVFAAFLNPGDEVIVMEPFFDQYISNITMNGG 140
>SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 28.7 bits (61), Expect = 0.94
Identities = 12/41 (29%), Positives = 20/41 (48%)
Frame = +1
Query: 544 PGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLKST 666
P + SC+ + NMGA +G + Y+ ++ LK T
Sbjct: 474 PALMATSCSAFMLLRNMGASVGISIGAVIYDQQLTTLLKGT 514
>SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 28.7 bits (61), Expect = 0.94
Identities = 12/41 (29%), Positives = 20/41 (48%)
Frame = +1
Query: 544 PGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLKST 666
P + SC+ + NMGA +G + Y+ ++ LK T
Sbjct: 474 PALIATSCSAFMLLRNMGASVGISMGAVIYDQQLTTLLKGT 514
>SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 404
Score = 27.5 bits (58), Expect = 2.2
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = -2
Query: 433 DADNFRTLQLPRDVGHYVHSISTTDT 356
D+ N +LP D+ HY H IS DT
Sbjct: 186 DSQNPSQYRLPDDLVHYFHGISRGDT 211
>SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 527
Score = 26.6 bits (56), Expect = 3.8
Identities = 19/71 (26%), Positives = 27/71 (38%)
Frame = -2
Query: 553 PPRVRGTPRWRRYLPHSQDAGHLQDHIFRTRPAGEFTGQFDADNFRTLQLPRDVGHYVHS 374
PP + P LP + + H DH F+T A D D + RDV S
Sbjct: 243 PPNMFEPPDEDHQLPSAANNSHNHDHAFQTAEAVGVADSIDPDWHQWPDDLRDVSSPKES 302
Query: 373 ISTTDTNTQST 341
D+ ++ T
Sbjct: 303 DGLNDSWSRQT 313
>SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase
Pef1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 288
Score = 25.8 bits (54), Expect = 6.6
Identities = 11/39 (28%), Positives = 18/39 (46%)
Frame = -3
Query: 552 HPGSVVLHDGAGTSLTVRMPATFKITSFGLVQPVSLPVN 436
H V+ D +L + K+ FGL + + +PVN
Sbjct: 118 HENRVLHRDLKPQNLLINSRGELKLADFGLARSIGIPVN 156
>SPCC16A11.13 |usp106|luc7|U1 snRNP-associated protein
Usp106|Schizosaccharomyces pombe|chr 3|||Manual
Length = 264
Score = 25.8 bits (54), Expect = 6.6
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +3
Query: 108 IHCDHLIEAQVGGEKDLARAKDLNKEVYKFLE 203
IH DH IE + + LA+ K +N V F+E
Sbjct: 124 IHTDHSIEVIITEMEALAKRKLVNDAVKHFIE 155
>SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1562
Score = 25.8 bits (54), Expect = 6.6
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = +1
Query: 634 NSRMEAYLKSTGRHDIAATANSYKHLLTPDNK 729
NS ++ LKS+ + + TANS +LTP K
Sbjct: 88 NSPSDSRLKSSFKSVLELTANSMVSVLTPSTK 119
>SPBC2D10.07c |||mitochondrial inner membrane peptidase complex
catalytic subunit|Schizosaccharomyces pombe|chr
2|||Manual
Length = 157
Score = 25.4 bits (53), Expect = 8.8
Identities = 13/52 (25%), Positives = 23/52 (44%), Gaps = 1/52 (1%)
Frame = -3
Query: 645 HAGVVREHASGSADLSAHVADCR-HACTRDVVHPGSVVLHDGAGTSLTVRMP 493
H R + G +SA +D + H C R + PG + D ++ + +P
Sbjct: 53 HGRFARSCSVGDVVVSAKPSDSKQHVCKRIIGMPGDTIYVDPTSSNKKITIP 104
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,198,161
Number of Sequences: 5004
Number of extensions: 67910
Number of successful extensions: 228
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 228
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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