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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021820
         (752 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g35830.1 68417.m05090 aconitate hydratase, cytoplasmic / citr...    51   7e-07
At4g26970.1 68417.m03881 aconitate hydratase, cytoplasmic, putat...    51   9e-07
At2g05710.1 68415.m00611 aconitate hydratase, cytoplasmic, putat...    50   2e-06
At4g13430.1 68417.m02096 aconitase family protein / aconitate hy...    40   0.002
At3g50590.1 68416.m05533 transducin family protein / WD-40 repea...    29   3.3  
At5g59220.1 68418.m07422 protein phosphatase 2C, putative / PP2C...    28   7.7  
At2g15680.1 68415.m01795 calmodulin-related protein, putative si...    28   7.7  

>At4g35830.1 68417.m05090 aconitate hydratase, cytoplasmic / citrate
           hydro-lyase / aconitase (ACO) identical to SP|Q42560
           Aconitate hydratase, cytoplasmic (EC 4.2.1.3) (Citrate
           hydro-lyase) (Aconitase) {Arabidopsis thaliana};
           contains Pfam profiles PF00330: Aconitase family
           (aconitate hydratase), PF00694: Aconitase C-terminal
           domain
          Length = 898

 Score = 51.2 bits (117), Expect = 7e-07
 Identities = 25/56 (44%), Positives = 30/56 (53%)
 Frame = +1

Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLKSTGRHD 678
           G  G  VE+HG G+  +S    ATI NM  E GAT   FP +     YL+ TGR D
Sbjct: 276 GVVGKFVEFHGEGMRELSLADRATIANMSPEYGATMGFFPVDHVTLQYLRLTGRSD 331



 Score = 39.9 bits (89), Expect = 0.002
 Identities = 22/68 (32%), Positives = 31/68 (45%)
 Frame = +2

Query: 299 MIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKLTGWTSPKDV 478
           ++GTDSHT                +A   M   P  +  P V+G KLTGKL    +  D+
Sbjct: 205 VVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLTGKLRDGMTATDL 264

Query: 479 ILKVAGIL 502
           +L V  +L
Sbjct: 265 VLTVTQML 272


>At4g26970.1 68417.m03881 aconitate hydratase, cytoplasmic, putative
           / citrate hydro-lyase/aconitase, putative strong
           similarity to SP|P49608 Aconitate hydratase, cytoplasmic
           (EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase)
           {Cucurbita maxima}; contains Pfam profiles PF00330:
           Aconitase family (aconitate hydratase), PF00694:
           Aconitase C-terminal domain
          Length = 995

 Score = 50.8 bits (116), Expect = 9e-07
 Identities = 25/56 (44%), Positives = 30/56 (53%)
 Frame = +1

Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLKSTGRHD 678
           G  G  VE++G G+  +S    ATI NM  E GAT   FP +     YLK TGR D
Sbjct: 373 GVVGKFVEFYGEGMSELSLADRATIANMSPEYGATMGFFPVDHVTLEYLKLTGRSD 428



 Score = 39.1 bits (87), Expect = 0.003
 Identities = 25/91 (27%), Positives = 40/91 (43%), Gaps = 2/91 (2%)
 Frame = +2

Query: 236 EARLWYFHQIILEN--YAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWEL 409
           +  L Y  +++  +  + +P  + +GTDSHT                +A   M   P  +
Sbjct: 280 QVNLEYLGRVVFNSKGFLYPDSV-VGTDSHTTMIDGLGVAGWGVGGIEAEAAMLGQPMSM 338

Query: 410 KCPKVIGVKLTGKLTGWTSPKDVILKVAGIL 502
             P V+G KL GKL    +  D++L V  IL
Sbjct: 339 VLPGVVGFKLDGKLKEGVTATDLVLTVTQIL 369


>At2g05710.1 68415.m00611 aconitate hydratase, cytoplasmic, putative
           / citrate hydro-lyase/aconitase, putative nearly
           identical to SP|P49608 Aconitate hydratase, cytoplasmic
           (EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase)
           {Cucurbita maxima}; contains Pfam profiles PF00330:
           Aconitase family (aconitate hydratase), PF00694:
           Aconitase C-terminal domain
          Length = 990

 Score = 49.6 bits (113), Expect = 2e-06
 Identities = 25/56 (44%), Positives = 30/56 (53%)
 Frame = +1

Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLKSTGRHD 678
           G  G  VE++G G+  +S    ATI NM  E GAT   FP +     YLK TGR D
Sbjct: 368 GVVGKFVEFYGNGMSGLSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSD 423



 Score = 37.9 bits (84), Expect = 0.007
 Identities = 20/68 (29%), Positives = 30/68 (44%)
 Frame = +2

Query: 299 MIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKLTGWTSPKDV 478
           ++GTDSHT                +A   M   P  +  P V+G KL GK+    +  D+
Sbjct: 297 VVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLAGKMRNGVTATDL 356

Query: 479 ILKVAGIL 502
           +L V  +L
Sbjct: 357 VLTVTQML 364


>At4g13430.1 68417.m02096 aconitase family protein / aconitate
           hydratase family protein contains Pfam profile PF00330:
           Aconitase family (aconitate hydratase
          Length = 509

 Score = 39.9 bits (89), Expect = 0.002
 Identities = 23/79 (29%), Positives = 37/79 (46%)
 Frame = +2

Query: 272 ENYAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKL 451
           E +  PG +++GTDSHT                DA  V+      LK P  +   L G++
Sbjct: 186 EGHCRPGEVLLGTDSHTCTAGAFGQFATGIGNTDAGFVLGTGKILLKVPPTMRFILDGEM 245

Query: 452 TGWTSPKDVILKVAGILTV 508
             +   KD+IL++ G ++V
Sbjct: 246 PSYLQAKDLILQIIGEISV 264


>At3g50590.1 68416.m05533 transducin family protein / WD-40 repeat
            family protein contains 3 WD-40 repeats (PF00400); some
            similarity to s-tomosyn isoform (GI:4689231)[Rattus
            norvegicus]; contains non-consensus AT-AC splice sites at
            intron 18
          Length = 1606

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 16/62 (25%), Positives = 29/62 (46%)
 Frame = -2

Query: 250  PEPGFQKPTPYLAPAVSKNLYTSLLRSLALAKSFSPPTWASIK*SQ*MVDGTATRGRPEE 71
            P+P  Q   P +A  + ++ + + + S+ +AK+  PP     K  Q      A +G P +
Sbjct: 1230 PQPVSQPAPPGVAAPIPEDFFQNTIPSVEVAKTLPPPGTYLSKMDQAARAAIAAQGGPNQ 1289

Query: 70   IN 65
             N
Sbjct: 1290 AN 1291


>At5g59220.1 68418.m07422 protein phosphatase 2C, putative / PP2C,
           putative ABA induced protein phosphatase 2C, Fagus
           sylvatica, EMBL:FSY277743
          Length = 413

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 12/35 (34%), Positives = 19/35 (54%)
 Frame = +1

Query: 571 GMATICNMGAEIGATTSVFPYNSRMEAYLKSTGRH 675
           G+A++C    E+    +V P+ SR +    STG H
Sbjct: 113 GVASVCGRRREMEDAVAVHPFFSRHQTEYSSTGFH 147


>At2g15680.1 68415.m01795 calmodulin-related protein, putative
           similar to calmodulin-related protein 2, touch-induced
           SP:P25070 from [Arabidopsis thaliana]
          Length = 187

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 11/21 (52%), Positives = 16/21 (76%), Gaps = 1/21 (4%)
 Frame = -3

Query: 135 GLRSSD-HNEWWTVQLHGEGR 76
           G+RSSD  N +WT  L+G+G+
Sbjct: 116 GIRSSDIRNSFWTFDLNGDGK 136


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,642,560
Number of Sequences: 28952
Number of extensions: 396132
Number of successful extensions: 1090
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1061
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1090
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1672953192
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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