BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021820 (752 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g35830.1 68417.m05090 aconitate hydratase, cytoplasmic / citr... 51 7e-07 At4g26970.1 68417.m03881 aconitate hydratase, cytoplasmic, putat... 51 9e-07 At2g05710.1 68415.m00611 aconitate hydratase, cytoplasmic, putat... 50 2e-06 At4g13430.1 68417.m02096 aconitase family protein / aconitate hy... 40 0.002 At3g50590.1 68416.m05533 transducin family protein / WD-40 repea... 29 3.3 At5g59220.1 68418.m07422 protein phosphatase 2C, putative / PP2C... 28 7.7 At2g15680.1 68415.m01795 calmodulin-related protein, putative si... 28 7.7 >At4g35830.1 68417.m05090 aconitate hydratase, cytoplasmic / citrate hydro-lyase / aconitase (ACO) identical to SP|Q42560 Aconitate hydratase, cytoplasmic (EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase) {Arabidopsis thaliana}; contains Pfam profiles PF00330: Aconitase family (aconitate hydratase), PF00694: Aconitase C-terminal domain Length = 898 Score = 51.2 bits (117), Expect = 7e-07 Identities = 25/56 (44%), Positives = 30/56 (53%) Frame = +1 Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLKSTGRHD 678 G G VE+HG G+ +S ATI NM E GAT FP + YL+ TGR D Sbjct: 276 GVVGKFVEFHGEGMRELSLADRATIANMSPEYGATMGFFPVDHVTLQYLRLTGRSD 331 Score = 39.9 bits (89), Expect = 0.002 Identities = 22/68 (32%), Positives = 31/68 (45%) Frame = +2 Query: 299 MIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKLTGWTSPKDV 478 ++GTDSHT +A M P + P V+G KLTGKL + D+ Sbjct: 205 VVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLTGKLRDGMTATDL 264 Query: 479 ILKVAGIL 502 +L V +L Sbjct: 265 VLTVTQML 272 >At4g26970.1 68417.m03881 aconitate hydratase, cytoplasmic, putative / citrate hydro-lyase/aconitase, putative strong similarity to SP|P49608 Aconitate hydratase, cytoplasmic (EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase) {Cucurbita maxima}; contains Pfam profiles PF00330: Aconitase family (aconitate hydratase), PF00694: Aconitase C-terminal domain Length = 995 Score = 50.8 bits (116), Expect = 9e-07 Identities = 25/56 (44%), Positives = 30/56 (53%) Frame = +1 Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLKSTGRHD 678 G G VE++G G+ +S ATI NM E GAT FP + YLK TGR D Sbjct: 373 GVVGKFVEFYGEGMSELSLADRATIANMSPEYGATMGFFPVDHVTLEYLKLTGRSD 428 Score = 39.1 bits (87), Expect = 0.003 Identities = 25/91 (27%), Positives = 40/91 (43%), Gaps = 2/91 (2%) Frame = +2 Query: 236 EARLWYFHQIILEN--YAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWEL 409 + L Y +++ + + +P + +GTDSHT +A M P + Sbjct: 280 QVNLEYLGRVVFNSKGFLYPDSV-VGTDSHTTMIDGLGVAGWGVGGIEAEAAMLGQPMSM 338 Query: 410 KCPKVIGVKLTGKLTGWTSPKDVILKVAGIL 502 P V+G KL GKL + D++L V IL Sbjct: 339 VLPGVVGFKLDGKLKEGVTATDLVLTVTQIL 369 >At2g05710.1 68415.m00611 aconitate hydratase, cytoplasmic, putative / citrate hydro-lyase/aconitase, putative nearly identical to SP|P49608 Aconitate hydratase, cytoplasmic (EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase) {Cucurbita maxima}; contains Pfam profiles PF00330: Aconitase family (aconitate hydratase), PF00694: Aconitase C-terminal domain Length = 990 Score = 49.6 bits (113), Expect = 2e-06 Identities = 25/56 (44%), Positives = 30/56 (53%) Frame = +1 Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLKSTGRHD 678 G G VE++G G+ +S ATI NM E GAT FP + YLK TGR D Sbjct: 368 GVVGKFVEFYGNGMSGLSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSD 423 Score = 37.9 bits (84), Expect = 0.007 Identities = 20/68 (29%), Positives = 30/68 (44%) Frame = +2 Query: 299 MIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKLTGWTSPKDV 478 ++GTDSHT +A M P + P V+G KL GK+ + D+ Sbjct: 297 VVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLAGKMRNGVTATDL 356 Query: 479 ILKVAGIL 502 +L V +L Sbjct: 357 VLTVTQML 364 >At4g13430.1 68417.m02096 aconitase family protein / aconitate hydratase family protein contains Pfam profile PF00330: Aconitase family (aconitate hydratase Length = 509 Score = 39.9 bits (89), Expect = 0.002 Identities = 23/79 (29%), Positives = 37/79 (46%) Frame = +2 Query: 272 ENYAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKL 451 E + PG +++GTDSHT DA V+ LK P + L G++ Sbjct: 186 EGHCRPGEVLLGTDSHTCTAGAFGQFATGIGNTDAGFVLGTGKILLKVPPTMRFILDGEM 245 Query: 452 TGWTSPKDVILKVAGILTV 508 + KD+IL++ G ++V Sbjct: 246 PSYLQAKDLILQIIGEISV 264 >At3g50590.1 68416.m05533 transducin family protein / WD-40 repeat family protein contains 3 WD-40 repeats (PF00400); some similarity to s-tomosyn isoform (GI:4689231)[Rattus norvegicus]; contains non-consensus AT-AC splice sites at intron 18 Length = 1606 Score = 29.1 bits (62), Expect = 3.3 Identities = 16/62 (25%), Positives = 29/62 (46%) Frame = -2 Query: 250 PEPGFQKPTPYLAPAVSKNLYTSLLRSLALAKSFSPPTWASIK*SQ*MVDGTATRGRPEE 71 P+P Q P +A + ++ + + + S+ +AK+ PP K Q A +G P + Sbjct: 1230 PQPVSQPAPPGVAAPIPEDFFQNTIPSVEVAKTLPPPGTYLSKMDQAARAAIAAQGGPNQ 1289 Query: 70 IN 65 N Sbjct: 1290 AN 1291 >At5g59220.1 68418.m07422 protein phosphatase 2C, putative / PP2C, putative ABA induced protein phosphatase 2C, Fagus sylvatica, EMBL:FSY277743 Length = 413 Score = 27.9 bits (59), Expect = 7.7 Identities = 12/35 (34%), Positives = 19/35 (54%) Frame = +1 Query: 571 GMATICNMGAEIGATTSVFPYNSRMEAYLKSTGRH 675 G+A++C E+ +V P+ SR + STG H Sbjct: 113 GVASVCGRRREMEDAVAVHPFFSRHQTEYSSTGFH 147 >At2g15680.1 68415.m01795 calmodulin-related protein, putative similar to calmodulin-related protein 2, touch-induced SP:P25070 from [Arabidopsis thaliana] Length = 187 Score = 27.9 bits (59), Expect = 7.7 Identities = 11/21 (52%), Positives = 16/21 (76%), Gaps = 1/21 (4%) Frame = -3 Query: 135 GLRSSD-HNEWWTVQLHGEGR 76 G+RSSD N +WT L+G+G+ Sbjct: 116 GIRSSDIRNSFWTFDLNGDGK 136 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,642,560 Number of Sequences: 28952 Number of extensions: 396132 Number of successful extensions: 1090 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 1061 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1090 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1672953192 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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