Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021819
(728 letters)
Database: fruitfly
53,049 sequences; 24,988,368 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
DQ162845-1|ABA42953.1| 1715|Drosophila melanogaster TRF2 protein. 80 4e-15
AY069554-1|AAL39699.1| 632|Drosophila melanogaster LD27895p pro... 80 4e-15
AJ238442-1|CAB41475.1| 537|Drosophila melanogaster TBP-like fac... 80 4e-15
AF136569-1|AAD28784.1| 632|Drosophila melanogaster TATA box bin... 80 4e-15
AE014298-1172|AAF46368.2| 632|Drosophila melanogaster CG18009-P... 80 4e-15
AE014298-1171|AAF46369.3| 632|Drosophila melanogaster CG18009-P... 80 4e-15
U35147-1|AAA79092.1| 354|Drosophila melanogaster TATA-box bindi... 58 9e-09
U11718-1|AAA68629.1| 353|Drosophila melanogaster TATA-box bindi... 58 9e-09
M38388-1|AAA28926.1| 353|Drosophila melanogaster TATA-consensus... 58 9e-09
M38082-1|AAA28931.1| 353|Drosophila melanogaster TATA-box bindi... 58 9e-09
AY069663-1|AAL39808.1| 353|Drosophila melanogaster LD44083p pro... 58 9e-09
AE013599-3256|AAF46754.1| 353|Drosophila melanogaster CG9874-PA... 58 9e-09
X70838-1|CAA50186.1| 224|Drosophila melanogaster TBP-related fa... 47 2e-05
X70837-1|CAA50185.1| 224|Drosophila melanogaster TBP-related fa... 47 2e-05
AY128483-1|AAM75076.1| 224|Drosophila melanogaster RE61442p pro... 47 2e-05
AE014134-1392|AAF52600.1| 224|Drosophila melanogaster CG7562-PA... 47 2e-05
AE014134-2459|AAF53372.1| 377|Drosophila melanogaster CG16876-P... 31 1.2
BT021949-1|AAX51654.1| 819|Drosophila melanogaster LD12033p pro... 31 2.1
AE014296-2720|AAF49474.1| 819|Drosophila melanogaster CG16807-P... 31 2.1
AY069725-1|AAL39870.1| 321|Drosophila melanogaster LP03067p pro... 29 6.5
AE014296-2375|AAF49748.1| 321|Drosophila melanogaster CG7924-PA... 29 6.5
>DQ162845-1|ABA42953.1| 1715|Drosophila melanogaster TRF2 protein.
Length = 1715
Score = 79.8 bits (188), Expect = 4e-15
Identities = 55/137 (40%), Positives = 73/137 (53%), Gaps = 7/137 (5%)
Frame = +3
Query: 333 QPAVDEEEETPEIDIMINNVVCSFSVKCHLNLRQIXLNGVNVEFRRENGMVTMKXXXXXX 512
QP D E E +DI+INNVVCSFSV CHL LR+I L G NVE+RRENGMVTMK
Sbjct: 1274 QPIADNEHE---LDIVINNVVCSFSVGCHLKLREIALQGSNVEYRRENGMVTMKLRHPYT 1330
Query: 513 XXXF---GRQDA*RARAPPV-KIKRR---LLLDVMHAPYRNSALKCASATFV*SMY*ETC 671
GR A + + K+ R L + P R + + TC
Sbjct: 1331 TASIWSSGRITCTGATSESMAKVAARRYARCLGKLGFPTRFLNFRIVNVL-------GTC 1383
Query: 672 RMPFGIRIISFSKKYKE 722
MP+ I+I++FS++++E
Sbjct: 1384 SMPWAIKIVNFSERHRE 1400
Score = 71.3 bits (167), Expect = 1e-12
Identities = 32/38 (84%), Positives = 34/38 (89%)
Frame = +2
Query: 509 TTASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TTASIWSSGR+TCTGATSE AK+AARRYAR L KLGF
Sbjct: 1330 TTASIWSSGRITCTGATSESMAKVAARRYARCLGKLGF 1367
>AY069554-1|AAL39699.1| 632|Drosophila melanogaster LD27895p
protein.
Length = 632
Score = 79.8 bits (188), Expect = 4e-15
Identities = 55/137 (40%), Positives = 73/137 (53%), Gaps = 7/137 (5%)
Frame = +3
Query: 333 QPAVDEEEETPEIDIMINNVVCSFSVKCHLNLRQIXLNGVNVEFRRENGMVTMKXXXXXX 512
QP D E E +DI+INNVVCSFSV CHL LR+I L G NVE+RRENGMVTMK
Sbjct: 191 QPIADNEHE---LDIVINNVVCSFSVGCHLKLREIALQGSNVEYRRENGMVTMKLRHPYT 247
Query: 513 XXXF---GRQDA*RARAPPV-KIKRR---LLLDVMHAPYRNSALKCASATFV*SMY*ETC 671
GR A + + K+ R L + P R + + TC
Sbjct: 248 TASIWSSGRITCTGATSESMAKVAARRYARCLGKLGFPTRFLNFRIVNVL-------GTC 300
Query: 672 RMPFGIRIISFSKKYKE 722
MP+ I+I++FS++++E
Sbjct: 301 SMPWAIKIVNFSERHRE 317
Score = 71.3 bits (167), Expect = 1e-12
Identities = 32/38 (84%), Positives = 34/38 (89%)
Frame = +2
Query: 509 TTASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TTASIWSSGR+TCTGATSE AK+AARRYAR L KLGF
Sbjct: 247 TTASIWSSGRITCTGATSESMAKVAARRYARCLGKLGF 284
>AJ238442-1|CAB41475.1| 537|Drosophila melanogaster TBP-like factor
protein.
Length = 537
Score = 79.8 bits (188), Expect = 4e-15
Identities = 55/137 (40%), Positives = 73/137 (53%), Gaps = 7/137 (5%)
Frame = +3
Query: 333 QPAVDEEEETPEIDIMINNVVCSFSVKCHLNLRQIXLNGVNVEFRRENGMVTMKXXXXXX 512
QP D E E +DI+INNVVCSFSV CHL LR+I L G NVE+RRENGMVTMK
Sbjct: 96 QPIADNEHE---LDIVINNVVCSFSVGCHLKLREIALQGSNVEYRRENGMVTMKLRHPYT 152
Query: 513 XXXF---GRQDA*RARAPPV-KIKRR---LLLDVMHAPYRNSALKCASATFV*SMY*ETC 671
GR A + + K+ R L + P R + + TC
Sbjct: 153 TASIWSSGRITCTGATSESMAKVAARRYARCLGKLGFPTRFLNFRIVNVL-------GTC 205
Query: 672 RMPFGIRIISFSKKYKE 722
MP+ I+I++FS++++E
Sbjct: 206 SMPWAIKIVNFSERHRE 222
Score = 71.3 bits (167), Expect = 1e-12
Identities = 32/38 (84%), Positives = 34/38 (89%)
Frame = +2
Query: 509 TTASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TTASIWSSGR+TCTGATSE AK+AARRYAR L KLGF
Sbjct: 152 TTASIWSSGRITCTGATSESMAKVAARRYARCLGKLGF 189
>AF136569-1|AAD28784.1| 632|Drosophila melanogaster TATA box
binding protein-relatedfactor 2 protein.
Length = 632
Score = 79.8 bits (188), Expect = 4e-15
Identities = 55/137 (40%), Positives = 73/137 (53%), Gaps = 7/137 (5%)
Frame = +3
Query: 333 QPAVDEEEETPEIDIMINNVVCSFSVKCHLNLRQIXLNGVNVEFRRENGMVTMKXXXXXX 512
QP D E E +DI+INNVVCSFSV CHL LR+I L G NVE+RRENGMVTMK
Sbjct: 191 QPIADNEHE---LDIVINNVVCSFSVGCHLKLREIALQGSNVEYRRENGMVTMKLRHPYT 247
Query: 513 XXXF---GRQDA*RARAPPV-KIKRR---LLLDVMHAPYRNSALKCASATFV*SMY*ETC 671
GR A + + K+ R L + P R + + TC
Sbjct: 248 TASIWSSGRITCTGATSESMAKVAARRYARCLGKLGFPTRFLNFRIVNVL-------GTC 300
Query: 672 RMPFGIRIISFSKKYKE 722
MP+ I+I++FS++++E
Sbjct: 301 SMPWAIKIVNFSERHRE 317
Score = 71.3 bits (167), Expect = 1e-12
Identities = 32/38 (84%), Positives = 34/38 (89%)
Frame = +2
Query: 509 TTASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TTASIWSSGR+TCTGATSE AK+AARRYAR L KLGF
Sbjct: 247 TTASIWSSGRITCTGATSESMAKVAARRYARCLGKLGF 284
>AE014298-1172|AAF46368.2| 632|Drosophila melanogaster CG18009-PD,
isoform D protein.
Length = 632
Score = 79.8 bits (188), Expect = 4e-15
Identities = 55/137 (40%), Positives = 73/137 (53%), Gaps = 7/137 (5%)
Frame = +3
Query: 333 QPAVDEEEETPEIDIMINNVVCSFSVKCHLNLRQIXLNGVNVEFRRENGMVTMKXXXXXX 512
QP D E E +DI+INNVVCSFSV CHL LR+I L G NVE+RRENGMVTMK
Sbjct: 191 QPIADNEHE---LDIVINNVVCSFSVGCHLKLREIALQGSNVEYRRENGMVTMKLRHPYT 247
Query: 513 XXXF---GRQDA*RARAPPV-KIKRR---LLLDVMHAPYRNSALKCASATFV*SMY*ETC 671
GR A + + K+ R L + P R + + TC
Sbjct: 248 TASIWSSGRITCTGATSESMAKVAARRYARCLGKLGFPTRFLNFRIVNVL-------GTC 300
Query: 672 RMPFGIRIISFSKKYKE 722
MP+ I+I++FS++++E
Sbjct: 301 SMPWAIKIVNFSERHRE 317
Score = 71.3 bits (167), Expect = 1e-12
Identities = 32/38 (84%), Positives = 34/38 (89%)
Frame = +2
Query: 509 TTASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TTASIWSSGR+TCTGATSE AK+AARRYAR L KLGF
Sbjct: 247 TTASIWSSGRITCTGATSESMAKVAARRYARCLGKLGF 284
>AE014298-1171|AAF46369.3| 632|Drosophila melanogaster CG18009-PA,
isoform A protein.
Length = 632
Score = 79.8 bits (188), Expect = 4e-15
Identities = 55/137 (40%), Positives = 73/137 (53%), Gaps = 7/137 (5%)
Frame = +3
Query: 333 QPAVDEEEETPEIDIMINNVVCSFSVKCHLNLRQIXLNGVNVEFRRENGMVTMKXXXXXX 512
QP D E E +DI+INNVVCSFSV CHL LR+I L G NVE+RRENGMVTMK
Sbjct: 191 QPIADNEHE---LDIVINNVVCSFSVGCHLKLREIALQGSNVEYRRENGMVTMKLRHPYT 247
Query: 513 XXXF---GRQDA*RARAPPV-KIKRR---LLLDVMHAPYRNSALKCASATFV*SMY*ETC 671
GR A + + K+ R L + P R + + TC
Sbjct: 248 TASIWSSGRITCTGATSESMAKVAARRYARCLGKLGFPTRFLNFRIVNVL-------GTC 300
Query: 672 RMPFGIRIISFSKKYKE 722
MP+ I+I++FS++++E
Sbjct: 301 SMPWAIKIVNFSERHRE 317
Score = 71.3 bits (167), Expect = 1e-12
Identities = 32/38 (84%), Positives = 34/38 (89%)
Frame = +2
Query: 509 TTASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TTASIWSSGR+TCTGATSE AK+AARRYAR L KLGF
Sbjct: 247 TTASIWSSGRITCTGATSESMAKVAARRYARCLGKLGF 284
>U35147-1|AAA79092.1| 354|Drosophila melanogaster TATA-box binding
protein protein.
Length = 354
Score = 58.4 bits (135), Expect = 9e-09
Identities = 25/38 (65%), Positives = 33/38 (86%)
Frame = +2
Query: 509 TTASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TTA I+SSG++ CTGA SED +++AAR+YAR +QKLGF
Sbjct: 224 TTALIFSSGKMVCTGAKSEDDSRLAARKYARIIQKLGF 261
>U11718-1|AAA68629.1| 353|Drosophila melanogaster TATA-box binding
protein protein.
Length = 353
Score = 58.4 bits (135), Expect = 9e-09
Identities = 25/38 (65%), Positives = 33/38 (86%)
Frame = +2
Query: 509 TTASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TTA I+SSG++ CTGA SED +++AAR+YAR +QKLGF
Sbjct: 223 TTALIFSSGKMVCTGAKSEDDSRLAARKYARIIQKLGF 260
>M38388-1|AAA28926.1| 353|Drosophila melanogaster
TATA-consensus-binding transcriptionfactor TFII protein.
Length = 353
Score = 58.4 bits (135), Expect = 9e-09
Identities = 25/38 (65%), Positives = 33/38 (86%)
Frame = +2
Query: 509 TTASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TTA I+SSG++ CTGA SED +++AAR+YAR +QKLGF
Sbjct: 223 TTALIFSSGKMVCTGAKSEDDSRLAARKYARIIQKLGF 260
>M38082-1|AAA28931.1| 353|Drosophila melanogaster TATA-box binding
protein protein.
Length = 353
Score = 58.4 bits (135), Expect = 9e-09
Identities = 25/38 (65%), Positives = 33/38 (86%)
Frame = +2
Query: 509 TTASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TTA I+SSG++ CTGA SED +++AAR+YAR +QKLGF
Sbjct: 223 TTALIFSSGKMVCTGAKSEDDSRLAARKYARIIQKLGF 260
>AY069663-1|AAL39808.1| 353|Drosophila melanogaster LD44083p
protein.
Length = 353
Score = 58.4 bits (135), Expect = 9e-09
Identities = 25/38 (65%), Positives = 33/38 (86%)
Frame = +2
Query: 509 TTASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TTA I+SSG++ CTGA SED +++AAR+YAR +QKLGF
Sbjct: 223 TTALIFSSGKMVCTGAKSEDDSRLAARKYARIIQKLGF 260
>AE013599-3256|AAF46754.1| 353|Drosophila melanogaster CG9874-PA
protein.
Length = 353
Score = 58.4 bits (135), Expect = 9e-09
Identities = 25/38 (65%), Positives = 33/38 (86%)
Frame = +2
Query: 509 TTASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TTA I+SSG++ CTGA SED +++AAR+YAR +QKLGF
Sbjct: 223 TTALIFSSGKMVCTGAKSEDDSRLAARKYARIIQKLGF 260
>X70838-1|CAA50186.1| 224|Drosophila melanogaster TBP-related
factor protein.
Length = 224
Score = 47.2 bits (107), Expect = 2e-05
Identities = 21/37 (56%), Positives = 29/37 (78%)
Frame = +2
Query: 512 TASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TA I+ +G+V CTGA +E +A I +R++AR LQKLGF
Sbjct: 96 TALIFRTGKVICTGARNEIEADIGSRKFARILQKLGF 132
Score = 36.3 bits (80), Expect = 0.043
Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 2/57 (3%)
Frame = +3
Query: 321 LAPTQPAVDEEEETPEIDIMINNVVCSFSVKCHLNLRQIXLNGVNVEF--RRENGMV 485
L P QP E + + +I + N+V +FSV C L+L+ I N E+ +R G++
Sbjct: 31 LQPQQPVALVEPKDAQHEIRLQNIVATFSVNCELDLKAINSRTRNSEYSPKRFRGVI 87
>X70837-1|CAA50185.1| 224|Drosophila melanogaster TBP-related
factor protein.
Length = 224
Score = 47.2 bits (107), Expect = 2e-05
Identities = 21/37 (56%), Positives = 29/37 (78%)
Frame = +2
Query: 512 TASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TA I+ +G+V CTGA +E +A I +R++AR LQKLGF
Sbjct: 96 TALIFRTGKVICTGARNEIEADIGSRKFARILQKLGF 132
Score = 36.3 bits (80), Expect = 0.043
Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 2/57 (3%)
Frame = +3
Query: 321 LAPTQPAVDEEEETPEIDIMINNVVCSFSVKCHLNLRQIXLNGVNVEF--RRENGMV 485
L P QP E + + +I + N+V +FSV C L+L+ I N E+ +R G++
Sbjct: 31 LQPQQPVALVEPKDAQHEIRLQNIVATFSVNCELDLKAINSRTRNSEYSPKRFRGVI 87
>AY128483-1|AAM75076.1| 224|Drosophila melanogaster RE61442p
protein.
Length = 224
Score = 47.2 bits (107), Expect = 2e-05
Identities = 21/37 (56%), Positives = 29/37 (78%)
Frame = +2
Query: 512 TASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TA I+ +G+V CTGA +E +A I +R++AR LQKLGF
Sbjct: 96 TALIFRTGKVICTGARNEIEADIGSRKFARILQKLGF 132
Score = 36.3 bits (80), Expect = 0.043
Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 2/57 (3%)
Frame = +3
Query: 321 LAPTQPAVDEEEETPEIDIMINNVVCSFSVKCHLNLRQIXLNGVNVEF--RRENGMV 485
L P QP E + + +I + N+V +FSV C L+L+ I N E+ +R G++
Sbjct: 31 LQPQQPVALVEPKDAQHEIRLQNIVATFSVNCELDLKAINSRTRNSEYSPKRFRGVI 87
>AE014134-1392|AAF52600.1| 224|Drosophila melanogaster CG7562-PA
protein.
Length = 224
Score = 47.2 bits (107), Expect = 2e-05
Identities = 21/37 (56%), Positives = 29/37 (78%)
Frame = +2
Query: 512 TASIWSSGRVTCTGATSEDQAKIAARRYARALQKLGF 622
TA I+ +G+V CTGA +E +A I +R++AR LQKLGF
Sbjct: 96 TALIFRTGKVICTGARNEIEADIGSRKFARILQKLGF 132
Score = 36.3 bits (80), Expect = 0.043
Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 2/57 (3%)
Frame = +3
Query: 321 LAPTQPAVDEEEETPEIDIMINNVVCSFSVKCHLNLRQIXLNGVNVEF--RRENGMV 485
L P QP E + + +I + N+V +FSV C L+L+ I N E+ +R G++
Sbjct: 31 LQPQQPVALVEPKDAQHEIRLQNIVATFSVNCELDLKAINSRTRNSEYSPKRFRGVI 87
>AE014134-2459|AAF53372.1| 377|Drosophila melanogaster CG16876-PA
protein.
Length = 377
Score = 31.5 bits (68), Expect = 1.2
Identities = 17/56 (30%), Positives = 27/56 (48%)
Frame = +3
Query: 237 QCPNTYVPDHEYCETTAQTPSQRQAITELAPTQPAVDEEEETPEIDIMINNVVCSF 404
QC + YVP+ E E T +R + ++ A+D+++E I I V C F
Sbjct: 280 QCSDGYVPE-ESGEATGVLSCRRTLLDQILGLNEAIDDDDELNPWTIPIIGVACGF 334
>BT021949-1|AAX51654.1| 819|Drosophila melanogaster LD12033p
protein.
Length = 819
Score = 30.7 bits (66), Expect = 2.1
Identities = 16/53 (30%), Positives = 29/53 (54%), Gaps = 3/53 (5%)
Frame = +1
Query: 130 VNHSVVVMATLIQENGIKELSKGTHGVVANHGISTHSVPTH---MCQTMNIAR 279
V HSVV + +Q +G+++ G G G +T+S P + +C+ +N+ R
Sbjct: 380 VRHSVVGLVNFLQHHGVRKAQDGISG--GGSGGTTNSNPKYKISLCRDLNVRR 430
>AE014296-2720|AAF49474.1| 819|Drosophila melanogaster CG16807-PA
protein.
Length = 819
Score = 30.7 bits (66), Expect = 2.1
Identities = 16/53 (30%), Positives = 29/53 (54%), Gaps = 3/53 (5%)
Frame = +1
Query: 130 VNHSVVVMATLIQENGIKELSKGTHGVVANHGISTHSVPTH---MCQTMNIAR 279
V HSVV + +Q +G+++ G G G +T+S P + +C+ +N+ R
Sbjct: 380 VRHSVVGLVNFLQHHGVRKAQDGISG--GGSGGTTNSNPKYKISLCRDLNVRR 430
>AY069725-1|AAL39870.1| 321|Drosophila melanogaster LP03067p
protein.
Length = 321
Score = 29.1 bits (62), Expect = 6.5
Identities = 19/71 (26%), Positives = 30/71 (42%), Gaps = 2/71 (2%)
Frame = +1
Query: 46 LLSFSKNENLNSYSHQNIKIYNQCWKGDVN--HSVVVMATLIQENGIKELSKGTHGVVAN 219
LLS K L + + I + G V+ VVV T+ +N ++ + + N
Sbjct: 37 LLSNGKPVILTNQEREEIATVQEIKPGKVHVVQEVVVPPTVKSDNKVRVTRSVSDSGIPN 96
Query: 220 HGISTHSVPTH 252
HGI +P H
Sbjct: 97 HGIPNPGIPNH 107
>AE014296-2375|AAF49748.1| 321|Drosophila melanogaster CG7924-PA
protein.
Length = 321
Score = 29.1 bits (62), Expect = 6.5
Identities = 19/71 (26%), Positives = 30/71 (42%), Gaps = 2/71 (2%)
Frame = +1
Query: 46 LLSFSKNENLNSYSHQNIKIYNQCWKGDVN--HSVVVMATLIQENGIKELSKGTHGVVAN 219
LLS K L + + I + G V+ VVV T+ +N ++ + + N
Sbjct: 37 LLSNGKPVILTNQEREEIATVQEIKPGKVHVVQEVVVPPTVKSDNKVRVTRSVSDSGIPN 96
Query: 220 HGISTHSVPTH 252
HGI +P H
Sbjct: 97 HGIPNPGIPNH 107
Database: fruitfly
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 24,988,368
Number of sequences in database: 53,049
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 31,819,283
Number of Sequences: 53049
Number of extensions: 663960
Number of successful extensions: 2256
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 2120
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2256
length of database: 24,988,368
effective HSP length: 83
effective length of database: 20,585,301
effective search space used: 3273062859
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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