BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021818 (779 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch... 140 2e-34 SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 118 1e-27 SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 43 6e-05 SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc... 40 4e-04 SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomy... 29 0.75 SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces... 27 2.3 SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces ... 27 4.0 SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch... 27 4.0 SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c... 27 4.0 SPBC2D10.07c |||mitochondrial inner membrane peptidase complex c... 27 4.0 SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase Pef1|... 26 7.0 SPCC16A11.13 |usp106|luc7|U1 snRNP-associated protein Usp106|Sch... 26 7.0 >SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr 1|||Manual Length = 778 Score = 140 bits (339), Expect = 2e-34 Identities = 65/85 (76%), Positives = 70/85 (82%) Frame = +2 Query: 257 HQIILENYAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVK 436 HQI+LENYAFPG L+IGTDSHTPN ADAVDVMAN+PWELKCPKVIGVK Sbjct: 170 HQIVLENYAFPGGLLIGTDSHTPNAGGLGMVAIGVGGADAVDVMANLPWELKCPKVIGVK 229 Query: 437 LTGKLTGWTSPKDVILKVAGILTVR 511 LTG+L GWTSPKDVILKVAGILTV+ Sbjct: 230 LTGQLKGWTSPKDVILKVAGILTVK 254 Score = 133 bits (322), Expect = 2e-32 Identities = 61/84 (72%), Positives = 70/84 (83%) Frame = +3 Query: 3 LRLRPDRVAMQDATAQMAMLQFISSGLPRVAVPSTIHCDHLIEAQVGGEKDLARAKDLNK 182 L+LRPDRVA QDATAQMA+LQF+S+G+P VAVP T+HCDHLIEA GG DL RA NK Sbjct: 85 LKLRPDRVACQDATAQMAILQFMSAGMPEVAVPVTVHCDHLIEAYEGGPIDLERANVTNK 144 Query: 183 EVYKFLETAGAKYGVGFWKPGSGI 254 EVY FL+TA AKY +GFW+PGSGI Sbjct: 145 EVYDFLQTACAKYNIGFWRPGSGI 168 Score = 92.7 bits (220), Expect = 5e-20 Identities = 39/51 (76%), Positives = 45/51 (88%) Frame = +1 Query: 508 EGGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLK 660 +GGTGAIVEY GPGV+ +SCTGM TICNMGAEIGATTS+FP+N RM YL+ Sbjct: 254 KGGTGAIVEYFGPGVESLSCTGMGTICNMGAEIGATTSIFPFNPRMSEYLR 304 >SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 118 bits (283), Expect = 1e-27 Identities = 53/85 (62%), Positives = 62/85 (72%) Frame = +2 Query: 257 HQIILENYAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVK 436 HQI+LENYA PG +M+GTDSHTPN ADAVD M N PWELK PK+IGV Sbjct: 174 HQIVLENYAAPGGMMLGTDSHTPNAGGLGMIAIGVGGADAVDAMTNTPWELKAPKIIGVN 233 Query: 437 LTGKLTGWTSPKDVILKVAGILTVR 511 LTG ++GWT+PKD+IL +AG LTVR Sbjct: 234 LTGAMSGWTTPKDLILHLAGKLTVR 258 Score = 106 bits (255), Expect = 3e-24 Identities = 46/84 (54%), Positives = 62/84 (73%) Frame = +3 Query: 3 LRLRPDRVAMQDATAQMAMLQFISSGLPRVAVPSTIHCDHLIEAQVGGEKDLARAKDLNK 182 L+L PDRVAMQDA+AQMA+LQF++ GL + +P++IHCDHLI G D+ + NK Sbjct: 89 LKLNPDRVAMQDASAQMALLQFMTCGLEKTMIPASIHCDHLIVGHRGANSDIPDSIANNK 148 Query: 183 EVYKFLETAGAKYGVGFWKPGSGI 254 E++ FL++A KYG+ FW PGSGI Sbjct: 149 EIFDFLQSAAKKYGIQFWGPGSGI 172 Score = 85.0 bits (201), Expect = 1e-17 Identities = 35/49 (71%), Positives = 40/49 (81%) Frame = +1 Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYL 657 GGTG I+EY GPGV +SCTGMAT+CNMGAE+GATTS+FPY M YL Sbjct: 259 GGTGHIIEYFGPGVASLSCTGMATVCNMGAEVGATTSIFPYTDSMRRYL 307 >SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces pombe|chr 1|||Manual Length = 721 Score = 42.7 bits (96), Expect = 6e-05 Identities = 26/88 (29%), Positives = 39/88 (44%), Gaps = 1/88 (1%) Frame = +2 Query: 257 HQIILEN-YAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGV 433 HQI++E YA PG + + +DSH+ DA + A + P + V Sbjct: 163 HQIMVEQGYAMPGSMAVASDSHSNTYGGVGCLGTPIVRTDAAAIWATGQTWWQIPPIARV 222 Query: 434 KLTGKLTGWTSPKDVILKVAGILTVREV 517 L G+L S KD+I+ + G EV Sbjct: 223 NLVGQLPKGLSGKDIIVSLCGAFNHDEV 250 >SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 758 Score = 39.9 bits (89), Expect = 4e-04 Identities = 16/53 (30%), Positives = 29/53 (54%) Frame = +1 Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLKFEP 669 GGTG+++E+ G ++ +S ++CNM E GA + ++ Y+K P Sbjct: 207 GGTGSVIEFCGEAIEGLSMEARMSMCNMSIEAGARAGMIAPDATTFEYVKNRP 259 Score = 31.9 bits (69), Expect = 0.11 Identities = 17/77 (22%), Positives = 34/77 (44%) Frame = +2 Query: 272 ENYAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKL 451 + + PG ++ DSHT ++ V+A + K + +++ GKL Sbjct: 127 QGFTLPGTTLVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTILQRKSKNMRIRVNGKL 186 Query: 452 TGWTSPKDVILKVAGIL 502 + KD+IL + G++ Sbjct: 187 PEGIASKDLILHIIGVI 203 >SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomyces pombe|chr 1|||Manual Length = 421 Score = 29.1 bits (62), Expect = 0.75 Identities = 16/48 (33%), Positives = 26/48 (54%), Gaps = 3/48 (6%) Frame = -1 Query: 236 PETDSIFSSGSL*KLVYFFVEVLSPGQILLASDLGFDQV---ITMNGG 102 P+T+ + ++G+ F L+PG ++ + FDQ ITMNGG Sbjct: 93 PDTEIVVTAGANEGFFSVFAAFLNPGDEVIVMEPFFDQYISNITMNGG 140 >SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 404 Score = 27.5 bits (58), Expect = 2.3 Identities = 12/26 (46%), Positives = 15/26 (57%) Frame = -2 Query: 433 DADNFRTLQLPRDVGHYVHSISTTDT 356 D+ N +LP D+ HY H IS DT Sbjct: 186 DSQNPSQYRLPDDLVHYFHGISRGDT 211 >SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces pombe|chr 2|||Manual Length = 527 Score = 26.6 bits (56), Expect = 4.0 Identities = 19/71 (26%), Positives = 27/71 (38%) Frame = -2 Query: 553 PPRVRGTPRWRRYLPHSQDAGHLQDHIFRTRPAGEFTGQFDADNFRTLQLPRDVGHYVHS 374 PP + P LP + + H DH F+T A D D + RDV S Sbjct: 243 PPNMFEPPDEDHQLPSAANNSHNHDHAFQTAEAVGVADSIDPDWHQWPDDLRDVSSPKES 302 Query: 373 ISTTDTNTQST 341 D+ ++ T Sbjct: 303 DGLNDSWSRQT 313 >SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 589 Score = 26.6 bits (56), Expect = 4.0 Identities = 11/39 (28%), Positives = 19/39 (48%) Frame = +1 Query: 544 PGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLK 660 P + SC+ + NMGA +G + Y+ ++ LK Sbjct: 474 PALMATSCSAFMLLRNMGASVGISIGAVIYDQQLTTLLK 512 >SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 589 Score = 26.6 bits (56), Expect = 4.0 Identities = 11/39 (28%), Positives = 19/39 (48%) Frame = +1 Query: 544 PGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLK 660 P + SC+ + NMGA +G + Y+ ++ LK Sbjct: 474 PALIATSCSAFMLLRNMGASVGISMGAVIYDQQLTTLLK 512 >SPBC2D10.07c |||mitochondrial inner membrane peptidase complex catalytic subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 157 Score = 26.6 bits (56), Expect = 4.0 Identities = 15/59 (25%), Positives = 25/59 (42%), Gaps = 1/59 (1%) Frame = -3 Query: 666 FELKVSFHAGVVREHASGSADLSAHVADCR-HACTRDVVHPGSVVLHDGAGTSLTVRMP 493 F L H R + G +SA +D + H C R + PG + D ++ + +P Sbjct: 46 FVLLDKLHGRFARSCSVGDVVVSAKPSDSKQHVCKRIIGMPGDTIYVDPTSSNKKITIP 104 >SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase Pef1|Schizosaccharomyces pombe|chr 3|||Manual Length = 288 Score = 25.8 bits (54), Expect = 7.0 Identities = 11/39 (28%), Positives = 18/39 (46%) Frame = -3 Query: 552 HPGSVVLHDGAGTSLTVRMPATFKITSFGLVQPVSLPVN 436 H V+ D +L + K+ FGL + + +PVN Sbjct: 118 HENRVLHRDLKPQNLLINSRGELKLADFGLARSIGIPVN 156 >SPCC16A11.13 |usp106|luc7|U1 snRNP-associated protein Usp106|Schizosaccharomyces pombe|chr 3|||Manual Length = 264 Score = 25.8 bits (54), Expect = 7.0 Identities = 13/32 (40%), Positives = 18/32 (56%) Frame = +3 Query: 108 IHCDHLIEAQVGGEKDLARAKDLNKEVYKFLE 203 IH DH IE + + LA+ K +N V F+E Sbjct: 124 IHTDHSIEVIITEMEALAKRKLVNDAVKHFIE 155 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,375,035 Number of Sequences: 5004 Number of extensions: 70599 Number of successful extensions: 226 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 204 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 226 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 377352472 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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