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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021818
         (779 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch...   140   2e-34
SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr...   118   1e-27
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc...    43   6e-05
SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc...    40   4e-04
SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomy...    29   0.75 
SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces...    27   2.3  
SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces ...    27   4.0  
SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch...    27   4.0  
SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c...    27   4.0  
SPBC2D10.07c |||mitochondrial inner membrane peptidase complex c...    27   4.0  
SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase Pef1|...    26   7.0  
SPCC16A11.13 |usp106|luc7|U1 snRNP-associated protein Usp106|Sch...    26   7.0  

>SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 778

 Score =  140 bits (339), Expect = 2e-34
 Identities = 65/85 (76%), Positives = 70/85 (82%)
 Frame = +2

Query: 257 HQIILENYAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVK 436
           HQI+LENYAFPG L+IGTDSHTPN             ADAVDVMAN+PWELKCPKVIGVK
Sbjct: 170 HQIVLENYAFPGGLLIGTDSHTPNAGGLGMVAIGVGGADAVDVMANLPWELKCPKVIGVK 229

Query: 437 LTGKLTGWTSPKDVILKVAGILTVR 511
           LTG+L GWTSPKDVILKVAGILTV+
Sbjct: 230 LTGQLKGWTSPKDVILKVAGILTVK 254



 Score =  133 bits (322), Expect = 2e-32
 Identities = 61/84 (72%), Positives = 70/84 (83%)
 Frame = +3

Query: 3   LRLRPDRVAMQDATAQMAMLQFISSGLPRVAVPSTIHCDHLIEAQVGGEKDLARAKDLNK 182
           L+LRPDRVA QDATAQMA+LQF+S+G+P VAVP T+HCDHLIEA  GG  DL RA   NK
Sbjct: 85  LKLRPDRVACQDATAQMAILQFMSAGMPEVAVPVTVHCDHLIEAYEGGPIDLERANVTNK 144

Query: 183 EVYKFLETAGAKYGVGFWKPGSGI 254
           EVY FL+TA AKY +GFW+PGSGI
Sbjct: 145 EVYDFLQTACAKYNIGFWRPGSGI 168



 Score = 92.7 bits (220), Expect = 5e-20
 Identities = 39/51 (76%), Positives = 45/51 (88%)
 Frame = +1

Query: 508 EGGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLK 660
           +GGTGAIVEY GPGV+ +SCTGM TICNMGAEIGATTS+FP+N RM  YL+
Sbjct: 254 KGGTGAIVEYFGPGVESLSCTGMGTICNMGAEIGATTSIFPFNPRMSEYLR 304


>SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 905

 Score =  118 bits (283), Expect = 1e-27
 Identities = 53/85 (62%), Positives = 62/85 (72%)
 Frame = +2

Query: 257 HQIILENYAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVK 436
           HQI+LENYA PG +M+GTDSHTPN             ADAVD M N PWELK PK+IGV 
Sbjct: 174 HQIVLENYAAPGGMMLGTDSHTPNAGGLGMIAIGVGGADAVDAMTNTPWELKAPKIIGVN 233

Query: 437 LTGKLTGWTSPKDVILKVAGILTVR 511
           LTG ++GWT+PKD+IL +AG LTVR
Sbjct: 234 LTGAMSGWTTPKDLILHLAGKLTVR 258



 Score =  106 bits (255), Expect = 3e-24
 Identities = 46/84 (54%), Positives = 62/84 (73%)
 Frame = +3

Query: 3   LRLRPDRVAMQDATAQMAMLQFISSGLPRVAVPSTIHCDHLIEAQVGGEKDLARAKDLNK 182
           L+L PDRVAMQDA+AQMA+LQF++ GL +  +P++IHCDHLI    G   D+  +   NK
Sbjct: 89  LKLNPDRVAMQDASAQMALLQFMTCGLEKTMIPASIHCDHLIVGHRGANSDIPDSIANNK 148

Query: 183 EVYKFLETAGAKYGVGFWKPGSGI 254
           E++ FL++A  KYG+ FW PGSGI
Sbjct: 149 EIFDFLQSAAKKYGIQFWGPGSGI 172



 Score = 85.0 bits (201), Expect = 1e-17
 Identities = 35/49 (71%), Positives = 40/49 (81%)
 Frame = +1

Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYL 657
           GGTG I+EY GPGV  +SCTGMAT+CNMGAE+GATTS+FPY   M  YL
Sbjct: 259 GGTGHIIEYFGPGVASLSCTGMATVCNMGAEVGATTSIFPYTDSMRRYL 307


>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 721

 Score = 42.7 bits (96), Expect = 6e-05
 Identities = 26/88 (29%), Positives = 39/88 (44%), Gaps = 1/88 (1%)
 Frame = +2

Query: 257 HQIILEN-YAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGV 433
           HQI++E  YA PG + + +DSH+                DA  + A      + P +  V
Sbjct: 163 HQIMVEQGYAMPGSMAVASDSHSNTYGGVGCLGTPIVRTDAAAIWATGQTWWQIPPIARV 222

Query: 434 KLTGKLTGWTSPKDVILKVAGILTVREV 517
            L G+L    S KD+I+ + G     EV
Sbjct: 223 NLVGQLPKGLSGKDIIVSLCGAFNHDEV 250


>SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 758

 Score = 39.9 bits (89), Expect = 4e-04
 Identities = 16/53 (30%), Positives = 29/53 (54%)
 Frame = +1

Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLKFEP 669
           GGTG+++E+ G  ++ +S     ++CNM  E GA   +   ++    Y+K  P
Sbjct: 207 GGTGSVIEFCGEAIEGLSMEARMSMCNMSIEAGARAGMIAPDATTFEYVKNRP 259



 Score = 31.9 bits (69), Expect = 0.11
 Identities = 17/77 (22%), Positives = 34/77 (44%)
 Frame = +2

Query: 272 ENYAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKL 451
           + +  PG  ++  DSHT               ++   V+A      +  K + +++ GKL
Sbjct: 127 QGFTLPGTTLVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTILQRKSKNMRIRVNGKL 186

Query: 452 TGWTSPKDVILKVAGIL 502
               + KD+IL + G++
Sbjct: 187 PEGIASKDLILHIIGVI 203


>SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 421

 Score = 29.1 bits (62), Expect = 0.75
 Identities = 16/48 (33%), Positives = 26/48 (54%), Gaps = 3/48 (6%)
 Frame = -1

Query: 236 PETDSIFSSGSL*KLVYFFVEVLSPGQILLASDLGFDQV---ITMNGG 102
           P+T+ + ++G+       F   L+PG  ++  +  FDQ    ITMNGG
Sbjct: 93  PDTEIVVTAGANEGFFSVFAAFLNPGDEVIVMEPFFDQYISNITMNGG 140


>SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 404

 Score = 27.5 bits (58), Expect = 2.3
 Identities = 12/26 (46%), Positives = 15/26 (57%)
 Frame = -2

Query: 433 DADNFRTLQLPRDVGHYVHSISTTDT 356
           D+ N    +LP D+ HY H IS  DT
Sbjct: 186 DSQNPSQYRLPDDLVHYFHGISRGDT 211


>SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 527

 Score = 26.6 bits (56), Expect = 4.0
 Identities = 19/71 (26%), Positives = 27/71 (38%)
 Frame = -2

Query: 553 PPRVRGTPRWRRYLPHSQDAGHLQDHIFRTRPAGEFTGQFDADNFRTLQLPRDVGHYVHS 374
           PP +   P     LP + +  H  DH F+T  A       D D  +     RDV     S
Sbjct: 243 PPNMFEPPDEDHQLPSAANNSHNHDHAFQTAEAVGVADSIDPDWHQWPDDLRDVSSPKES 302

Query: 373 ISTTDTNTQST 341
               D+ ++ T
Sbjct: 303 DGLNDSWSRQT 313


>SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 589

 Score = 26.6 bits (56), Expect = 4.0
 Identities = 11/39 (28%), Positives = 19/39 (48%)
 Frame = +1

Query: 544 PGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLK 660
           P +   SC+    + NMGA +G +     Y+ ++   LK
Sbjct: 474 PALMATSCSAFMLLRNMGASVGISIGAVIYDQQLTTLLK 512


>SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 589

 Score = 26.6 bits (56), Expect = 4.0
 Identities = 11/39 (28%), Positives = 19/39 (48%)
 Frame = +1

Query: 544 PGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLK 660
           P +   SC+    + NMGA +G +     Y+ ++   LK
Sbjct: 474 PALIATSCSAFMLLRNMGASVGISMGAVIYDQQLTTLLK 512


>SPBC2D10.07c |||mitochondrial inner membrane peptidase complex
           catalytic subunit|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 157

 Score = 26.6 bits (56), Expect = 4.0
 Identities = 15/59 (25%), Positives = 25/59 (42%), Gaps = 1/59 (1%)
 Frame = -3

Query: 666 FELKVSFHAGVVREHASGSADLSAHVADCR-HACTRDVVHPGSVVLHDGAGTSLTVRMP 493
           F L    H    R  + G   +SA  +D + H C R +  PG  +  D   ++  + +P
Sbjct: 46  FVLLDKLHGRFARSCSVGDVVVSAKPSDSKQHVCKRIIGMPGDTIYVDPTSSNKKITIP 104


>SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase
           Pef1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 288

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 11/39 (28%), Positives = 18/39 (46%)
 Frame = -3

Query: 552 HPGSVVLHDGAGTSLTVRMPATFKITSFGLVQPVSLPVN 436
           H   V+  D    +L +      K+  FGL + + +PVN
Sbjct: 118 HENRVLHRDLKPQNLLINSRGELKLADFGLARSIGIPVN 156


>SPCC16A11.13 |usp106|luc7|U1 snRNP-associated protein
           Usp106|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 264

 Score = 25.8 bits (54), Expect = 7.0
 Identities = 13/32 (40%), Positives = 18/32 (56%)
 Frame = +3

Query: 108 IHCDHLIEAQVGGEKDLARAKDLNKEVYKFLE 203
           IH DH IE  +   + LA+ K +N  V  F+E
Sbjct: 124 IHTDHSIEVIITEMEALAKRKLVNDAVKHFIE 155


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,375,035
Number of Sequences: 5004
Number of extensions: 70599
Number of successful extensions: 226
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 226
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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