Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021818
(779 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35830.1 68417.m05090 aconitate hydratase, cytoplasmic / citr... 44 1e-04
At4g26970.1 68417.m03881 aconitate hydratase, cytoplasmic, putat... 43 2e-04
At2g05710.1 68415.m00611 aconitate hydratase, cytoplasmic, putat... 42 5e-04
At4g13430.1 68417.m02096 aconitase family protein / aconitate hy... 40 0.002
At3g50590.1 68416.m05533 transducin family protein / WD-40 repea... 29 3.5
At5g24360.1 68418.m02872 protein kinase family protein / Ire1 ho... 29 4.6
At5g50490.1 68418.m06254 CCAAT-box binding transcription factor ... 28 8.0
At2g15680.1 68415.m01795 calmodulin-related protein, putative si... 28 8.0
>At4g35830.1 68417.m05090 aconitate hydratase, cytoplasmic / citrate
hydro-lyase / aconitase (ACO) identical to SP|Q42560
Aconitate hydratase, cytoplasmic (EC 4.2.1.3) (Citrate
hydro-lyase) (Aconitase) {Arabidopsis thaliana};
contains Pfam profiles PF00330: Aconitase family
(aconitate hydratase), PF00694: Aconitase C-terminal
domain
Length = 898
Score = 44.0 bits (99), Expect = 1e-04
Identities = 22/57 (38%), Positives = 28/57 (49%)
Frame = +1
Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLKFEPDAHD 681
G G VE+HG G+ +S ATI NM E GAT FP + YL+ + D
Sbjct: 276 GVVGKFVEFHGEGMRELSLADRATIANMSPEYGATMGFFPVDHVTLQYLRLTGRSDD 332
Score = 39.9 bits (89), Expect = 0.002
Identities = 22/68 (32%), Positives = 31/68 (45%)
Frame = +2
Query: 299 MIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKLTGWTSPKDV 478
++GTDSHT +A M P + P V+G KLTGKL + D+
Sbjct: 205 VVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLTGKLRDGMTATDL 264
Query: 479 ILKVAGIL 502
+L V +L
Sbjct: 265 VLTVTQML 272
>At4g26970.1 68417.m03881 aconitate hydratase, cytoplasmic, putative
/ citrate hydro-lyase/aconitase, putative strong
similarity to SP|P49608 Aconitate hydratase, cytoplasmic
(EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase)
{Cucurbita maxima}; contains Pfam profiles PF00330:
Aconitase family (aconitate hydratase), PF00694:
Aconitase C-terminal domain
Length = 995
Score = 43.2 bits (97), Expect = 2e-04
Identities = 21/50 (42%), Positives = 26/50 (52%)
Frame = +1
Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLK 660
G G VE++G G+ +S ATI NM E GAT FP + YLK
Sbjct: 373 GVVGKFVEFYGEGMSELSLADRATIANMSPEYGATMGFFPVDHVTLEYLK 422
Score = 39.1 bits (87), Expect = 0.003
Identities = 25/91 (27%), Positives = 40/91 (43%), Gaps = 2/91 (2%)
Frame = +2
Query: 236 EARLWYFHQIILEN--YAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWEL 409
+ L Y +++ + + +P + +GTDSHT +A M P +
Sbjct: 280 QVNLEYLGRVVFNSKGFLYPDSV-VGTDSHTTMIDGLGVAGWGVGGIEAEAAMLGQPMSM 338
Query: 410 KCPKVIGVKLTGKLTGWTSPKDVILKVAGIL 502
P V+G KL GKL + D++L V IL
Sbjct: 339 VLPGVVGFKLDGKLKEGVTATDLVLTVTQIL 369
>At2g05710.1 68415.m00611 aconitate hydratase, cytoplasmic, putative
/ citrate hydro-lyase/aconitase, putative nearly
identical to SP|P49608 Aconitate hydratase, cytoplasmic
(EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase)
{Cucurbita maxima}; contains Pfam profiles PF00330:
Aconitase family (aconitate hydratase), PF00694:
Aconitase C-terminal domain
Length = 990
Score = 41.9 bits (94), Expect = 5e-04
Identities = 21/50 (42%), Positives = 26/50 (52%)
Frame = +1
Query: 511 GGTGAIVEYHGPGVDHISCTGMATICNMGAEIGATTSVFPYNSRMEAYLK 660
G G VE++G G+ +S ATI NM E GAT FP + YLK
Sbjct: 368 GVVGKFVEFYGNGMSGLSLADRATIANMSPEYGATMGFFPVDHVTLQYLK 417
Score = 37.9 bits (84), Expect = 0.007
Identities = 20/68 (29%), Positives = 30/68 (44%)
Frame = +2
Query: 299 MIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKLTGWTSPKDV 478
++GTDSHT +A M P + P V+G KL GK+ + D+
Sbjct: 297 VVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLAGKMRNGVTATDL 356
Query: 479 ILKVAGIL 502
+L V +L
Sbjct: 357 VLTVTQML 364
>At4g13430.1 68417.m02096 aconitase family protein / aconitate
hydratase family protein contains Pfam profile PF00330:
Aconitase family (aconitate hydratase
Length = 509
Score = 39.9 bits (89), Expect = 0.002
Identities = 23/79 (29%), Positives = 37/79 (46%)
Frame = +2
Query: 272 ENYAFPGLLMIGTDSHTPNXXXXXXXXXXXXXADAVDVMANIPWELKCPKVIGVKLTGKL 451
E + PG +++GTDSHT DA V+ LK P + L G++
Sbjct: 186 EGHCRPGEVLLGTDSHTCTAGAFGQFATGIGNTDAGFVLGTGKILLKVPPTMRFILDGEM 245
Query: 452 TGWTSPKDVILKVAGILTV 508
+ KD+IL++ G ++V
Sbjct: 246 PSYLQAKDLILQIIGEISV 264
>At3g50590.1 68416.m05533 transducin family protein / WD-40 repeat
family protein contains 3 WD-40 repeats (PF00400); some
similarity to s-tomosyn isoform (GI:4689231)[Rattus
norvegicus]; contains non-consensus AT-AC splice sites at
intron 18
Length = 1606
Score = 29.1 bits (62), Expect = 3.5
Identities = 16/62 (25%), Positives = 29/62 (46%)
Frame = -2
Query: 250 PEPGFQKPTPYLAPAVSKNLYTSLLRSLALAKSFSPPTWASIK*SQ*MVDGTATRGRPEE 71
P+P Q P +A + ++ + + + S+ +AK+ PP K Q A +G P +
Sbjct: 1230 PQPVSQPAPPGVAAPIPEDFFQNTIPSVEVAKTLPPPGTYLSKMDQAARAAIAAQGGPNQ 1289
Query: 70 IN 65
N
Sbjct: 1290 AN 1291
>At5g24360.1 68418.m02872 protein kinase family protein / Ire1
homolog-1 (IRE1-1) identical to Ire1 homolog-1
[Arabidopsis thaliana] GI:15277137; cDNA Ire1 homolog-1
GI:15277136;
Length = 881
Score = 28.7 bits (61), Expect = 4.6
Identities = 15/52 (28%), Positives = 29/52 (55%), Gaps = 2/52 (3%)
Frame = -1
Query: 764 IESNWFIGCLCCRAVEQVACRK--LAVAAMSWASGSNLR*ASMRELYGNTLV 615
++++ FIG L + +++ K +V + W +G ++ M ELY NT+V
Sbjct: 128 VDASEFIGTLPYTSTDRIVLGKKDTSVFLLDWKTGKLVKRYRMDELYSNTVV 179
>At5g50490.1 68418.m06254 CCAAT-box binding transcription factor
Hap5a, putative
Length = 186
Score = 27.9 bits (59), Expect = 8.0
Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Frame = +1
Query: 592 MGAEIGATTSVFPYNSRMEAYLKFEPDAHDIAATA-NFLQATC 717
M ++ FP SR++ +KF+PD IAA A N L C
Sbjct: 26 MEGDLNVKNHEFPI-SRIKRIMKFDPDVSMIAAEAPNLLSKAC 67
>At2g15680.1 68415.m01795 calmodulin-related protein, putative
similar to calmodulin-related protein 2, touch-induced
SP:P25070 from [Arabidopsis thaliana]
Length = 187
Score = 27.9 bits (59), Expect = 8.0
Identities = 11/21 (52%), Positives = 16/21 (76%), Gaps = 1/21 (4%)
Frame = -3
Query: 135 GLRSSD-HNEWWTVQLHGEGR 76
G+RSSD N +WT L+G+G+
Sbjct: 116 GIRSSDIRNSFWTFDLNGDGK 136
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,561,458
Number of Sequences: 28952
Number of extensions: 411055
Number of successful extensions: 1105
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1074
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1105
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1746037600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -