Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021816X
(536 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19900.1 68415.m02326 malate oxidoreductase, putative similar... 128 2e-30
At5g25880.1 68418.m03071 malate oxidoreductase, putative similar... 124 3e-29
At5g11670.1 68418.m01364 malate oxidoreductase, putative similar... 122 1e-28
At1g79750.1 68414.m09304 malate oxidoreductase, putative similar... 119 1e-27
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar... 100 1e-21
At2g13560.1 68415.m01495 malate oxidoreductase, putative similar... 94 5e-20
At3g24780.1 68416.m03110 hypothetical protein 28 4.5
At3g53540.1 68416.m05912 expressed protein 27 6.0
At3g07780.1 68416.m00949 expressed protein 27 6.0
At1g09790.1 68414.m01098 phytochelatin synthetase-related contai... 27 6.0
At1g08620.1 68414.m00955 transcription factor jumonji (jmj) fami... 27 6.0
At5g03415.1 68418.m00294 DPB-1 transcription factor, putative (D... 27 7.9
At1g51910.1 68414.m05851 protein kinase family protein contains ... 27 7.9
>At2g19900.1 68415.m02326 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P51615) {Vitis vinifera}
Length = 581
Score = 128 bits (310), Expect = 2e-30
Identities = 57/94 (60%), Positives = 74/94 (78%)
Frame = +3
Query: 228 RQIRKST*QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRG 407
RQ + +Y+ L L +RNE LFY+ + DNV E++PIVYTPTVG ACQKFG ++RRP+G
Sbjct: 90 RQYQFPLQKYMALTELQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKFGSIFRRPQG 149
Query: 408 LFITIHDKGHVYDVLKNWPETDVRAIVVTDGERI 509
LFI++ DKG + DVLKNWPE +++ IVVTDGERI
Sbjct: 150 LFISLKDKGKILDVLKNWPERNIQVIVVTDGERI 183
Score = 43.6 bits (98), Expect = 9e-05
Identities = 24/56 (42%), Positives = 33/56 (58%)
Frame = +1
Query: 82 SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
SG L+ P NKG+AFT +ER + GLLPP V Q+ Q + +I +Y+ PL
Sbjct: 41 SGYSLLRDPRYNKGLAFTEKERDTHYLRGLLPPVVLDQKLQEKRLLNNIRQYQFPL 96
>At5g25880.1 68418.m03071 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P12628) {Phaseolus vulgaris}
Length = 588
Score = 124 bits (300), Expect = 3e-29
Identities = 51/86 (59%), Positives = 71/86 (82%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+ LM L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G +YRRP+GL+I++ +K
Sbjct: 105 RYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLYISLKEK 164
Query: 432 GHVYDVLKNWPETDVRAIVVTDGERI 509
G + +VLKNWP+ ++ IVVTDGERI
Sbjct: 165 GKILEVLKNWPQRGIQVIVVTDGERI 190
Score = 44.0 bits (99), Expect = 6e-05
Identities = 25/60 (41%), Positives = 34/60 (56%)
Frame = +1
Query: 70 TSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
TS SG ++ P NKG+AFT +ER I GLLPP V +Q+ Q ++ +Y PL
Sbjct: 44 TSVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQDVQERKVMHNLRQYTVPL 103
>At5g11670.1 68418.m01364 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP|P12628) {Phaseolus vulgaris}
Length = 588
Score = 122 bits (295), Expect = 1e-28
Identities = 49/86 (56%), Positives = 72/86 (83%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 431
+Y+ LM L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G ++R+P+GL+I++++K
Sbjct: 105 RYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRKPQGLYISLNEK 164
Query: 432 GHVYDVLKNWPETDVRAIVVTDGERI 509
G + +VLKNWP+ ++ IVVTDGERI
Sbjct: 165 GKILEVLKNWPQRGIQVIVVTDGERI 190
Score = 42.7 bits (96), Expect = 1e-04
Identities = 23/60 (38%), Positives = 34/60 (56%)
Frame = +1
Query: 70 TSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
TS SG ++ P NKG+AFT +ER + GLLPP + +Q+ Q ++ +Y PL
Sbjct: 44 TSVASGYTLMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQDVQERKVMHNLRQYTVPL 103
>At1g79750.1 68414.m09304 malate oxidoreductase, putative similar to
malate oxidoreductase (NADP-dependent malic enzyme)
GB:P34105 (Populus balsamifera subsp. trichocarpa)
Length = 646
Score = 119 bits (287), Expect = 1e-27
Identities = 60/132 (45%), Positives = 87/132 (65%), Gaps = 2/132 (1%)
Frame = +3
Query: 120 GHGFYHRRASA--LGHTRVVTTTSQDARGAG*TMQALDRQIRKST*QYIYLMGLLDRNEH 293
G F HR A L T SQD + M L RQ + +Y+ +M L + NE
Sbjct: 119 GLAFSHRERDAHYLRGLLPPTVISQDLQVKK-IMHTL-RQYQVPLQKYMAMMDLQETNER 176
Query: 294 LFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETD 473
LFY+ + D+V E++P++YTPTVG ACQK+G ++ RP+GLFI++ +KG +++VL+NWPE +
Sbjct: 177 LFYKLLIDHVEELLPVIYTPTVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKN 236
Query: 474 VRAIVVTDGERI 509
++ IVVTDGERI
Sbjct: 237 IQVIVVTDGERI 248
Score = 44.0 bits (99), Expect = 6e-05
Identities = 23/56 (41%), Positives = 34/56 (60%)
Frame = +1
Query: 82 SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 249
SG L+ P NKG+AF+ ER + GLLPP V +Q+ QV+ ++ +Y+ PL
Sbjct: 106 SGYTLLRDPHHNKGLAFSHRERDAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPL 161
>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
tuberosum}
Length = 607
Score = 99.5 bits (237), Expect = 1e-21
Identities = 41/79 (51%), Positives = 55/79 (69%)
Frame = +3
Query: 273 LLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVL 452
L DRNE L+YR + DN+ + PI+YTPTVGL CQ + +YRRPRG++ + DKG + ++
Sbjct: 112 LHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMI 171
Query: 453 KNWPETDVRAIVVTDGERI 509
NWP V IV+TDG RI
Sbjct: 172 YNWPAPQVDMIVITDGSRI 190
Score = 46.0 bits (104), Expect = 2e-05
Identities = 26/58 (44%), Positives = 31/58 (53%)
Frame = +1
Query: 85 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNNT 258
G D L P NK F + ER LGI GLLPPRV T +Q C I+ + + NNT
Sbjct: 39 GADILHDPWFNKDTGFPLTERDRLGIRGLLPPRVMTCVQQ---CDRFIESFRSLENNT 93
>At2g13560.1 68415.m01495 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37221) {Solanum
tuberosum}
Length = 623
Score = 94.3 bits (224), Expect = 5e-20
Identities = 39/79 (49%), Positives = 55/79 (69%)
Frame = +3
Query: 273 LLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVL 452
L DRNE ++Y+ + +N+ E PIVYTPTVGL CQ + ++RRPRG++ + D+G + ++
Sbjct: 119 LHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMV 178
Query: 453 KNWPETDVRAIVVTDGERI 509
NWP V IVVTDG RI
Sbjct: 179 YNWPAEQVDMIVVTDGSRI 197
Score = 48.4 bits (110), Expect = 3e-06
Identities = 24/52 (46%), Positives = 29/52 (55%)
Frame = +1
Query: 85 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYE 240
GLD L P NKG AFT+ ER L + GLLPP V E+Q+ + R E
Sbjct: 46 GLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLE 97
>At3g24780.1 68416.m03110 hypothetical protein
Length = 715
Score = 27.9 bits (59), Expect = 4.5
Identities = 15/52 (28%), Positives = 22/52 (42%)
Frame = -1
Query: 170 NPCMPKS*RSSMVKAMPLLRPGCLRWSRPERPLVGCPSPEPYNPIRSRSISL 15
N C P + S +K + +R RP RP+ P P+R R + L
Sbjct: 52 NTCRPSVPKVSNIKKSTKVNVNPVRPVRPVRPIRPVRPVRPVRPVRRRRVDL 103
>At3g53540.1 68416.m05912 expressed protein
Length = 924
Score = 27.5 bits (58), Expect = 6.0
Identities = 13/44 (29%), Positives = 21/44 (47%)
Frame = -1
Query: 524 SRPDPDTFSVGYNNGTDVRLRPVLKNIVYMSLIVDRDEEAPWSA 393
SRP + YN+ +V + P L VYM+ + +P+ A
Sbjct: 579 SRPGSNKSHSSYNSSPEVSITPSLSKFVYMNDGIPSKSASPFKA 622
>At3g07780.1 68416.m00949 expressed protein
Length = 566
Score = 27.5 bits (58), Expect = 6.0
Identities = 11/26 (42%), Positives = 13/26 (50%)
Frame = +2
Query: 440 IRCS*ELAGDGRPCHCCNRRRTYLGL 517
I C EL DG PC C R+ + L
Sbjct: 200 IACQNELPADGCPCEICANRKGFCNL 225
>At1g09790.1 68414.m01098 phytochelatin synthetase-related contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 454
Score = 27.5 bits (58), Expect = 6.0
Identities = 10/35 (28%), Positives = 20/35 (57%)
Frame = +1
Query: 169 LLPPRVKTQEEQVELCKLSIDRYENPLNNTSILWG 273
+L P +K+ ++ S+ Y+N +N+T + WG
Sbjct: 328 VLHPNLKSVQQVFSFNYKSLTPYQNSINDTGMFWG 362
>At1g08620.1 68414.m00955 transcription factor jumonji (jmj) family
protein / zinc finger (C5HC2 type) family protein
contains Pfam domains, PF02375: jmjN domain, PF02373:
jmjC domain and PF02928: C5HC2 zinc finger
Length = 1183
Score = 27.5 bits (58), Expect = 6.0
Identities = 19/70 (27%), Positives = 33/70 (47%), Gaps = 2/70 (2%)
Frame = +1
Query: 52 SGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKL--S 225
+G G P S +G++ L+ G G FT+++ Q K++ + CK+ S
Sbjct: 218 NGMGDPCSASTGMNELETFGFEPGPGFTLKDFQKYADEFKAQYFKKSETSTDDKCKVDNS 277
Query: 226 IDRYENPLNN 255
ID +E L +
Sbjct: 278 IDCWEPALED 287
>At5g03415.1 68418.m00294 DPB-1 transcription factor, putative (DPB)
similar to Swiss-Prot:Q14186 transcription factor DP-1
[Homo sapiens]; contains Pfam profile PF02319:
Transcription factor E2F/dimerisation partner (TDP)
Length = 385
Score = 27.1 bits (57), Expect = 7.9
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +3
Query: 252 QYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPT 356
QY+ L L+ RNEHL+ A + +P + T
Sbjct: 218 QYVGLQNLIQRNEHLYSSGNAPSGGVALPFILVQT 252
>At1g51910.1 68414.m05851 protein kinase family protein contains
Serine/Threonine protein kinases active-site signature,
PROSITE:PS00108
Length = 876
Score = 27.1 bits (57), Expect = 7.9
Identities = 15/42 (35%), Positives = 20/42 (47%)
Frame = +1
Query: 61 GQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRV 186
G P L+ D +KH NK + I+E Q HG+ P V
Sbjct: 468 GNPKLNLTVPDSIKHRINNKSLKLIIDENQSSEKHGIKFPLV 509
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,278,555
Number of Sequences: 28952
Number of extensions: 302195
Number of successful extensions: 842
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 818
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 842
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 993966856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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