Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021812
(728 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 157 2e-39
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 157 2e-39
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 157 2e-39
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 44 2e-05
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.096
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 29 0.90
SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar... 27 2.1
SPAC683.02c ||SPAC694.01c|zf-CCHC type zinc finger protein|Schiz... 27 2.7
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 157 bits (381), Expect = 2e-39
Identities = 83/154 (53%), Positives = 102/154 (66%), Gaps = 1/154 (0%)
Frame = -3
Query: 726 DNVGFNVKNVSVKELCRGYVAGDSKNNPPKGCCRFYSSSHCA*PSWSNLKRLHTSLGLPH 547
DNVGFNVKNVSVK++ RG V GDSKN+PP GC F ++ + ++ + H
Sbjct: 304 DNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASF-TAQVIILNHPGQISAGYSPVLDCH 362
Query: 546 CPH-CLQICRNQRKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 370
H + K+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRF
Sbjct: 363 TAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRF 422
Query: 369 AVRDMRQTVAVGVIKAVNFKEAGGGKVTKAAEKA 268
AVRDMRQTVAVGVIKAV G KVTKAA KA
Sbjct: 423 AVRDMRQTVAVGVIKAVEKVAPGAAKVTKAAVKA 456
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 157 bits (381), Expect = 2e-39
Identities = 83/154 (53%), Positives = 102/154 (66%), Gaps = 1/154 (0%)
Frame = -3
Query: 726 DNVGFNVKNVSVKELCRGYVAGDSKNNPPKGCCRFYSSSHCA*PSWSNLKRLHTSLGLPH 547
DNVGFNVKNVSVK++ RG V GDSKN+PP GC F ++ + ++ + H
Sbjct: 304 DNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASF-TAQVIILNHPGQISAGYSPVLDCH 362
Query: 546 CPH-CLQICRNQRKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 370
H + K+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRF
Sbjct: 363 TAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRF 422
Query: 369 AVRDMRQTVAVGVIKAVNFKEAGGGKVTKAAEKA 268
AVRDMRQTVAVGVIKAV G KVTKAA KA
Sbjct: 423 AVRDMRQTVAVGVIKAVEKVAPGAAKVTKAAVKA 456
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 157 bits (381), Expect = 2e-39
Identities = 83/154 (53%), Positives = 102/154 (66%), Gaps = 1/154 (0%)
Frame = -3
Query: 726 DNVGFNVKNVSVKELCRGYVAGDSKNNPPKGCCRFYSSSHCA*PSWSNLKRLHTSLGLPH 547
DNVGFNVKNVSVK++ RG V GDSKN+PP GC F ++ + ++ + H
Sbjct: 304 DNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASF-TAQVIILNHPGQISAGYSPVLDCH 362
Query: 546 CPH-CLQICRNQRKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRF 370
H + K+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRF
Sbjct: 363 TAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRF 422
Query: 369 AVRDMRQTVAVGVIKAVNFKEAGGGKVTKAAEKA 268
AVRDMRQTVAVGVIKAV G KVTKAA KA
Sbjct: 423 AVRDMRQTVAVGVIKAVEKVAPGAAKVTKAAVKA 456
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 44.0 bits (99), Expect = 2e-05
Identities = 23/66 (34%), Positives = 38/66 (57%), Gaps = 1/66 (1%)
Frame = -3
Query: 510 KVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG- 334
K+D+ T + ++ P G I L P+C+E F+++ +GRF +RD TVAVG
Sbjct: 598 KLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGK 656
Query: 333 VIKAVN 316
V+K ++
Sbjct: 657 VVKILD 662
Score = 32.7 bits (71), Expect = 0.055
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = -1
Query: 623 FTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKL 504
F AQ+ +L P ++ GY+ V+ HTA FA++ KL
Sbjct: 560 FIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKL 599
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 31.9 bits (69), Expect = 0.096
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = -3
Query: 417 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 325
PLC+ +E P LGRF +R TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 28.7 bits (61), Expect = 0.90
Identities = 18/53 (33%), Positives = 30/53 (56%)
Frame = +2
Query: 440 MAASPDLMDFGLTSVDLPVRRSTFL*FLQICRQCGQCGNPRLVCNRLRFDQDG 598
++ +PDL D L+SVD P++ +T C + GN L+C+ F++DG
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNT----------CSESGNQYLLCD---FNRDG 66
>SPAC23A1.04c |mnl1||alpha mannosidase-like
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 27.5 bits (58), Expect = 2.1
Identities = 11/47 (23%), Positives = 24/47 (51%)
Frame = +1
Query: 430 QVDNGCISRLDGFWVDFSRFTSTTVNFSLISANLQAMWAVWQSKTGV 570
+V C + ++FS T+ T N ++ A ++VW+ ++G+
Sbjct: 198 EVTENCAAAASSLVLEFSMLTALTGNNKFKASAENAFFSVWKRRSGI 244
>SPAC683.02c ||SPAC694.01c|zf-CCHC type zinc finger
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 218
Score = 27.1 bits (57), Expect = 2.7
Identities = 13/28 (46%), Positives = 18/28 (64%), Gaps = 5/28 (17%)
Frame = -3
Query: 675 GYVAGDSKNNP----PKG-CCRFYSSSH 607
G+++G + NP PKG CC+F SS H
Sbjct: 133 GHLSGQCEQNPKGLYPKGGCCKFCSSVH 160
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,902,403
Number of Sequences: 5004
Number of extensions: 59041
Number of successful extensions: 177
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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