Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021807
(704 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 118 4e-27
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 118 4e-27
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 29 2.3
At2g43420.1 68415.m05396 3-beta hydroxysteroid dehydrogenase/iso... 29 3.0
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 29 4.0
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 29 4.0
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 28 5.2
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 28 6.9
At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp... 27 9.2
At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp... 27 9.2
At1g80480.1 68414.m09427 PRLI-interacting factor L, putative sim... 27 9.2
At1g76010.1 68414.m08825 expressed protein 27 9.2
At1g54140.1 68414.m06172 transcription initiation factor IID (TF... 27 9.2
At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to S... 27 9.2
At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to S... 27 9.2
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 118 bits (284), Expect = 4e-27
Identities = 55/91 (60%), Positives = 57/91 (62%)
Frame = +3
Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434
QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 67 QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMK 126
Query: 435 XXXXXXXXXXXXXXXXXQARGHIIERFPNFP 527
ARGH IE P P
Sbjct: 127 RHAIVSAIAATAVPALVMARGHKIENVPEMP 157
Score = 66.5 bits (155), Expect = 2e-11
Identities = 30/61 (49%), Positives = 41/61 (67%)
Frame = +2
Query: 512 IPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKG 691
+PE+PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I RKG
Sbjct: 153 VPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKG 212
Query: 692 P 694
P
Sbjct: 213 P 213
Score = 66.1 bits (154), Expect = 2e-11
Identities = 33/63 (52%), Positives = 41/63 (65%), Gaps = 2/63 (3%)
Frame = +1
Query: 76 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 249
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+A
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64
Query: 250 GHK 258
GH+
Sbjct: 65 GHQ 67
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 118 bits (284), Expect = 4e-27
Identities = 55/91 (60%), Positives = 57/91 (62%)
Frame = +3
Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434
QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 66 QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMK 125
Query: 435 XXXXXXXXXXXXXXXXXQARGHIIERFPNFP 527
ARGH IE P P
Sbjct: 126 RHAIVSAIAATAVPALVMARGHKIENVPEMP 156
Score = 68.1 bits (159), Expect = 5e-12
Identities = 33/66 (50%), Positives = 45/66 (68%), Gaps = 2/66 (3%)
Frame = +1
Query: 67 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 240
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 241 KEAGHK 258
K+AGH+
Sbjct: 61 KKAGHQ 66
Score = 66.5 bits (155), Expect = 2e-11
Identities = 30/61 (49%), Positives = 41/61 (67%)
Frame = +2
Query: 512 IPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKG 691
+PE+PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I RKG
Sbjct: 152 VPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKG 211
Query: 692 P 694
P
Sbjct: 212 P 212
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 29.5 bits (63), Expect = 2.3
Identities = 16/49 (32%), Positives = 29/49 (59%)
Frame = +1
Query: 91 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 237
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICI 397
>At2g43420.1 68415.m05396 3-beta hydroxysteroid
dehydrogenase/isomerase family protein contains Pfam
profile PF01073 3-beta hydroxysteroid
dehydrogenase/isomerase domain; similar to
NAD(P)-dependent steroid dehydrogenase from Homo sapiens
[SP|Q15738], Mus musculus [SP|Q9R1J0]
Length = 561
Score = 29.1 bits (62), Expect = 3.0
Identities = 18/56 (32%), Positives = 27/56 (48%), Gaps = 3/56 (5%)
Frame = -3
Query: 270 IRHWFVTSLLAHAVGLPRVLGHRNVNIIDQVRTYGRLEHEREGLGC---TLHRLAL 112
+R W S + +G PR V ++ V + + HE+EGLG T H+ AL
Sbjct: 263 VRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLKWTHEKEGLGSNYDTAHQYAL 318
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 28.7 bits (61), Expect = 4.0
Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 3/64 (4%)
Frame = +3
Query: 216 LEAALLREQGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAP 386
+ AL+R + G ++ +AE + + + ++P G G G+ G FGN GG F
Sbjct: 76 MAVALIRRRMG-AKPTAEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGD 134
Query: 387 TKPW 398
W
Sbjct: 135 ISKW 138
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 28.7 bits (61), Expect = 4.0
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = -3
Query: 570 CLVLLISWTLSATTRGSSGIFQ*CV 496
CLVLL+S +A T+ SGIF C+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFTGCL 33
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.3 bits (60), Expect = 5.2
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +1
Query: 142 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 237
P+PF K+P +PD++ H + NSR +C+
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCL 414
Score = 27.5 bits (58), Expect = 9.2
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +1
Query: 142 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 237
P+PF K+P +PD++ H + NS+ +C+
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCL 887
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 27.9 bits (59), Expect = 6.9
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -3
Query: 369 HHDTCYRRHPDRTYEYHHHGHAEF 298
HH Y H Y Y +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 844
Score = 27.5 bits (58), Expect = 9.2
Identities = 12/41 (29%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Frame = -3
Query: 381 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVRYPMIRH 262
R++ H + +H D + E+HHH H R+H + H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 843
Score = 27.5 bits (58), Expect = 9.2
Identities = 12/41 (29%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Frame = -3
Query: 381 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVRYPMIRH 262
R++ H + +H D + E+HHH H R+H + H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At1g80480.1 68414.m09427 PRLI-interacting factor L, putative
similar to PRLI-interacting factor L [Arabidopsis
thaliana] GI:11139268; contains Pfam profile PF02492:
Cobalamin synthesis protein/P47K
Length = 444
Score = 27.5 bits (58), Expect = 9.2
Identities = 9/28 (32%), Positives = 13/28 (46%)
Frame = -3
Query: 369 HHDTCYRRHPDRTYEYHHHGHAEFGRQH 286
HHD + H D +++ HH H H
Sbjct: 311 HHDHDHDHHHDHNHDHDHHHHDGHDHHH 338
>At1g76010.1 68414.m08825 expressed protein
Length = 350
Score = 27.5 bits (58), Expect = 9.2
Identities = 17/43 (39%), Positives = 18/43 (41%)
Frame = +3
Query: 240 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRG 368
QG + G GR R R RGGG R G G F N G
Sbjct: 303 QGRGGYDGPQGRGRGRGRGRGGRGRGGG--RGGDGGFNNRSDG 343
>At1g54140.1 68414.m06172 transcription initiation factor IID
(TFIID) 31 kDa subunit (TAFII-31) family protein
contains Pfam profile: PF02291 transcription initiation
factor IID, 31kD subunit
Length = 183
Score = 27.5 bits (58), Expect = 9.2
Identities = 16/47 (34%), Positives = 21/47 (44%)
Frame = +1
Query: 457 LLLPASQHSFRLEDTLLKDSRTSPGCSRQSPGDQQDQTGCHLPEAPQ 597
L++P S E+T + T PG S Q QQ QT + PQ
Sbjct: 126 LVIPKKSVSTEPEETEDDEEMTDPGQSSQEQQQQQQQTSDLPSQTPQ 172
>At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1050
Score = 27.5 bits (58), Expect = 9.2
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +3
Query: 240 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 374
+G S+ + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
>At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1048
Score = 27.5 bits (58), Expect = 9.2
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +3
Query: 240 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 374
+G S+ + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,179,710
Number of Sequences: 28952
Number of extensions: 325817
Number of successful extensions: 1121
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1039
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1110
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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