Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021793
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g69340.1 68414.m07956 appr-1-p processing enzyme family prote... 67 9e-12
At4g35750.1 68417.m05074 Rho-GTPase-activating protein-related c... 45 5e-05
At3g10210.1 68416.m01222 expressed protein similar to putative p... 33 0.23
At5g60190.1 68418.m07545 Ulp1 protease family protein low simila... 30 1.2
At4g05440.1 68417.m00826 temperature sensing protein-related con... 30 1.2
At1g23560.1 68414.m02964 expressed protein contains Pfam profile... 29 2.2
At1g30110.1 68414.m03680 diadenosine 5',5'''-P1,P4-tetraphosphat... 28 5.0
At5g58300.1 68418.m07298 leucine-rich repeat transmembrane prote... 27 8.8
At3g53090.1 68416.m05851 HECT-domain-containing protein / ubiqui... 27 8.8
At1g56560.1 68414.m06505 beta-fructofuranosidase, putative / inv... 27 8.8
>At1g69340.1 68414.m07956 appr-1-p processing enzyme family protein
contains Pfam domain PF01661: Appr-1-p processing enzyme
family
Length = 562
Score = 67.3 bits (157), Expect = 9e-12
Identities = 29/85 (34%), Positives = 47/85 (55%)
Frame = +2
Query: 257 GRPVVVFIGKWFPIGDIDXXXXXXXXXXXXDPIVRGDYVIAYFHTLASSANHPPFSWLKE 436
G PV+V +G F + +D +P+++ Y I YFH+ AS P W+K
Sbjct: 409 GHPVMVVVGAHFLLRCLDLERFVLYVIKEFEPVIQKPYSIVYFHSAASLQVQPDLGWMKR 468
Query: 437 VYTVLPYKYKKNLKAFYIVHPTFWL 511
+ +L K+++NL+A Y++HPTF L
Sbjct: 469 LEQILGRKHQRNLQAIYVLHPTFHL 493
Score = 36.3 bits (80), Expect = 0.019
Identities = 19/61 (31%), Positives = 35/61 (57%)
Frame = +1
Query: 484 LHCSSYVLAKMMTWWFTTFMAPAIKAKVHSLPGVEYLYSVMPRDQLEVPAFVTEYDMTIN 663
LH + ++ A ++T F F+ + KV + L+ +PR+QL +P FV ++D+ +N
Sbjct: 487 LHPTFHLKATILTMQF--FVDNVVWKKVVYADRLLQLFKYVPREQLTIPDFVFQHDLEVN 544
Query: 664 G 666
G
Sbjct: 545 G 545
>At4g35750.1 68417.m05074 Rho-GTPase-activating protein-related
contains weak similarity to Rho-GTPase-activating
protein 1 (GTPase-activating protein rhoOGAP)
(Rho-related small GTPase protein activator) (CDC42
GTPase-activating protein) (p50-rhoGAP)
(Swiss-Prot:Q07960) [Homo sapiens]
Length = 202
Score = 44.8 bits (101), Expect = 5e-05
Identities = 25/87 (28%), Positives = 39/87 (44%), Gaps = 2/87 (2%)
Frame = +2
Query: 245 RGSMGRPVVVFIGKWFPIGDIDXXXXXXXXXXXXDP-IVRGDYVIAYFHT-LASSANHPP 418
R GR ++ IGK+FP + P + R + + Y HT + S N P
Sbjct: 25 RDKRGRKILRIIGKFFPARFLSLDVLKKYLEEKIFPRLGRKPFAVLYVHTGVQRSENFPG 84
Query: 419 FSWLKEVYTVLPYKYKKNLKAFYIVHP 499
S L+ +Y +P + NL+ Y +HP
Sbjct: 85 ISALRAIYDAIPVNVRDNLQEVYFLHP 111
Score = 27.5 bits (58), Expect = 8.8
Identities = 10/30 (33%), Positives = 21/30 (70%)
Frame = +1
Query: 562 KVHSLPGVEYLYSVMPRDQLEVPAFVTEYD 651
K+ + V+YL+ + R+++E+P FV ++D
Sbjct: 134 KLRYISRVDYLWEHVRRNEIEMPEFVYDHD 163
>At3g10210.1 68416.m01222 expressed protein similar to putative
protein GB:CAA20045 [Arabidopsis thaliana]
Length = 237
Score = 32.7 bits (71), Expect = 0.23
Identities = 20/83 (24%), Positives = 32/83 (38%), Gaps = 2/83 (2%)
Frame = +2
Query: 257 GRPVVVFIGKWFPIGDIDXXXXXXXXXXXX-DPIVRGDYVIAYFH-TLASSANHPPFSWL 430
G + +GK+FP + + G + Y H T+ N P + L
Sbjct: 64 GNRIFRIVGKYFPARVVSAERLKKYISQKISNQCPEGPLCLVYMHSTVQKDDNSPGITIL 123
Query: 431 KEVYTVLPYKYKKNLKAFYIVHP 499
+ +Y LP K L+ Y +HP
Sbjct: 124 RWIYEDLPSDIKDRLQLVYFIHP 146
>At5g60190.1 68418.m07545 Ulp1 protease family protein low
similarity to sentrin/SUMO-specific protease [Homo
sapiens] GI:6906859; contains Pfam profile PF02902: Ulp1
protease family, C-terminal catalytic domain
Length = 226
Score = 30.3 bits (65), Expect = 1.2
Identities = 13/35 (37%), Positives = 23/35 (65%)
Frame = +1
Query: 400 VGEPSALLVAQGGVHGAALQIQEESESFLHCSSYV 504
V + S + VA+GG+H + L +E+ +F+H SY+
Sbjct: 92 VNDNSNVEVAEGGLHWSLLVYYKEANTFVHHDSYM 126
>At4g05440.1 68417.m00826 temperature sensing protein-related
contains weak similarity to D123 (GI:1236114) [Rattus
norvegicus]
Length = 333
Score = 30.3 bits (65), Expect = 1.2
Identities = 16/52 (30%), Positives = 22/52 (42%)
Frame = +1
Query: 526 WFTTFMAPAIKAKVHSLPGVEYLYSVMPRDQLEVPAFVTEYDMTINGLHYFQ 681
W+ F + IK K H LP Y + +P VT D N +H F+
Sbjct: 14 WYPRFKSLTIKTKFHKLPESFINYLIDDSGPFLLPHSVTNEDAMPNRVHNFE 65
>At1g23560.1 68414.m02964 expressed protein contains Pfam profile
PF02713: Domain of unknown function DUF220
Length = 332
Score = 29.5 bits (63), Expect = 2.2
Identities = 13/37 (35%), Positives = 17/37 (45%)
Frame = +1
Query: 7 KSEIILKV*ITNGP*SSYKTKQKTKWRWTLWQWNCQI 117
K I KV I GP + + KQ W++ W C I
Sbjct: 138 KENISRKVLIDEGPKQTVEVKQAAAWKFLWWDGTCPI 174
>At1g30110.1 68414.m03680 diadenosine 5',5'''-P1,P4-tetraphosphate
hydrolase, putative similar to diadenosine
5',5'''-P1,P4-tetraphosphate hydrolase GI:1888557 from
[Lupinus angustifolius], [Hordeum vulgare subsp.
vulgare] GI:2564253; contains Pfam profile PF00293:
NUDIX domain
Length = 175
Score = 28.3 bits (60), Expect = 5.0
Identities = 21/77 (27%), Positives = 38/77 (49%), Gaps = 4/77 (5%)
Frame = +1
Query: 373 DRVLSHAGLVGEPSALLVAQGGVHGA----ALQIQEESESFLHCSSYVLAKMMTWWFTTF 540
D ++ A + P A + QGG+ + ++E E S+ +++++ W T
Sbjct: 20 DNLVFVASRLNVPGAWQMPQGGIEDGEDPKSAAMRELQEETGVVSAEIVSEVPNW-LTYD 78
Query: 541 MAPAIKAKVHSLPGVEY 591
PA+KAKV+ L G E+
Sbjct: 79 FPPAVKAKVNRLWGGEW 95
>At5g58300.1 68418.m07298 leucine-rich repeat transmembrane protein
kinase, putative
Length = 654
Score = 27.5 bits (58), Expect = 8.8
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 565 VHSLPGVEYLYSVMPRDQLEVPAFVT 642
+HSLP ++Y+Y EVP+FV+
Sbjct: 132 IHSLPSLDYIYLQHNNFSGEVPSFVS 157
>At3g53090.1 68416.m05851 HECT-domain-containing protein /
ubiquitin-transferase family protein / IQ
calmodulin-binding motif-containing protein contains
Pfam profiles PF00632: HECT-domain
(ubiquitin-transferase), PF00612: IQ calmodulin-binding
motif
Length = 1142
Score = 27.5 bits (58), Expect = 8.8
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +1
Query: 448 AALQIQEESESFLHCSSYVLAKMMTWWFTTFMAP 549
AA++IQEE E+ L C S L K +W + + P
Sbjct: 61 AAIEIQEEWENLLSCHSVTLTK--SWVSSRVLRP 92
>At1g56560.1 68414.m06505 beta-fructofuranosidase, putative /
invertase, putative / saccharase, putative /
beta-fructosidase, putative similar to neutral invertase
[Daucus carota] GI:4200165; contains Pfam profile
PF04853: Plant neutral invertase
Length = 616
Score = 27.5 bits (58), Expect = 8.8
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +1
Query: 397 LVGEPSALLVAQGGVHGAALQIQEESESFLHCSSYVLA 510
LV + S ++ + G+HG L+IQ S L CS +L+
Sbjct: 304 LVTDGSCMIDRRMGIHGHPLEIQSLFYSALRCSREMLS 341
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,177,100
Number of Sequences: 28952
Number of extensions: 254975
Number of successful extensions: 651
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 633
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 650
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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