Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021792
(772 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g19950.3 68418.m02375 expressed protein 64 1e-10
At5g19950.2 68418.m02374 expressed protein 64 1e-10
At5g19950.1 68418.m02373 expressed protein 64 1e-10
At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative / aceta... 32 0.48
At5g35250.1 68418.m04179 hypothetical protein includes At2g05890... 30 1.5
At5g62310.1 68418.m07822 incomplete root hair elongation (IRE) /... 29 3.4
At5g58840.1 68418.m07373 subtilase family protein contains simil... 29 4.5
At5g65310.1 68418.m08216 homeobox-leucine zipper protein 5 (HB-5... 28 5.9
At3g54520.1 68416.m06033 hypothetical protein 28 5.9
At1g66810.1 68414.m07594 zinc finger (CCCH-type) family protein ... 28 5.9
At5g28750.1 68418.m03531 thylakoid assembly protein, putative si... 28 7.9
At5g07770.1 68418.m00889 formin homology 2 domain-containing pro... 28 7.9
At3g54670.1 68416.m06049 structural maintenance of chromosomes (... 28 7.9
>At5g19950.3 68418.m02375 expressed protein
Length = 441
Score = 64.1 bits (149), Expect = 1e-10
Identities = 34/88 (38%), Positives = 56/88 (63%), Gaps = 2/88 (2%)
Frame = +2
Query: 251 IKSPVVVQIQKIRNVSAPKANE--ESTSAPRMLKLTLHDGKVTCTGLEISHIPSLSINTP 424
++ P V+QI +R+V+ A E S++ R+LK L DGK + LE SHIP+++ +
Sbjct: 87 LQGPKVLQISYVRDVTRSSAEEFVGSSTGKRVLKFALTDGKTEISALEYSHIPTINNDVT 146
Query: 425 PGTKLLLKNEELEVCHGVVWLTPSVISV 508
PGTK+ L+N+ + + G+V LTP ++V
Sbjct: 147 PGTKVRLENKAV-IRDGLVCLTPKEVTV 173
Score = 28.3 bits (60), Expect = 5.9
Identities = 12/36 (33%), Positives = 19/36 (52%), Gaps = 1/36 (2%)
Frame = +1
Query: 508 MGGTVSHMIEKWELNRSLAKHTRGGIGAEG-GPPPW 612
+GG V + E+W++ + A R G GPPP+
Sbjct: 174 LGGYVQSLTEEWQMKKKYASLARSQESKAGDGPPPF 209
>At5g19950.2 68418.m02374 expressed protein
Length = 443
Score = 64.1 bits (149), Expect = 1e-10
Identities = 34/88 (38%), Positives = 56/88 (63%), Gaps = 2/88 (2%)
Frame = +2
Query: 251 IKSPVVVQIQKIRNVSAPKANE--ESTSAPRMLKLTLHDGKVTCTGLEISHIPSLSINTP 424
++ P V+QI +R+V+ A E S++ R+LK L DGK + LE SHIP+++ +
Sbjct: 87 LQGPKVLQISYVRDVTRSSAEEFVGSSTGKRVLKFALTDGKTEISALEYSHIPTINNDVT 146
Query: 425 PGTKLLLKNEELEVCHGVVWLTPSVISV 508
PGTK+ L+N+ + + G+V LTP ++V
Sbjct: 147 PGTKVRLENKAV-IRDGLVCLTPKEVTV 173
Score = 28.3 bits (60), Expect = 5.9
Identities = 12/36 (33%), Positives = 19/36 (52%), Gaps = 1/36 (2%)
Frame = +1
Query: 508 MGGTVSHMIEKWELNRSLAKHTRGGIGAEG-GPPPW 612
+GG V + E+W++ + A R G GPPP+
Sbjct: 174 LGGYVQSLTEEWQMKKKYASLARSQESKAGDGPPPF 209
>At5g19950.1 68418.m02373 expressed protein
Length = 443
Score = 64.1 bits (149), Expect = 1e-10
Identities = 34/88 (38%), Positives = 56/88 (63%), Gaps = 2/88 (2%)
Frame = +2
Query: 251 IKSPVVVQIQKIRNVSAPKANE--ESTSAPRMLKLTLHDGKVTCTGLEISHIPSLSINTP 424
++ P V+QI +R+V+ A E S++ R+LK L DGK + LE SHIP+++ +
Sbjct: 87 LQGPKVLQISYVRDVTRSSAEEFVGSSTGKRVLKFALTDGKTEISALEYSHIPTINNDVT 146
Query: 425 PGTKLLLKNEELEVCHGVVWLTPSVISV 508
PGTK+ L+N+ + + G+V LTP ++V
Sbjct: 147 PGTKVRLENKAV-IRDGLVCLTPKEVTV 173
Score = 28.3 bits (60), Expect = 5.9
Identities = 12/36 (33%), Positives = 19/36 (52%), Gaps = 1/36 (2%)
Frame = +1
Query: 508 MGGTVSHMIEKWELNRSLAKHTRGGIGAEG-GPPPW 612
+GG V + E+W++ + A R G GPPP+
Sbjct: 174 LGGYVQSLTEEWQMKKKYASLARSQESKAGDGPPPF 209
>At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative /
acetate-CoA ligase, putative similar to SP|P27550
(Escherichia coli) and gi:8439651 (Homo sapiens);
contains Pfam AMP-binding enzyme domain PF00501
Length = 693
Score = 31.9 bits (69), Expect = 0.48
Identities = 16/45 (35%), Positives = 20/45 (44%)
Frame = -2
Query: 609 WWRTSFSSYASSCVFGKTSVQFPFFNHMRYCSAHTDITDGVSHTT 475
WW+ S Y +SC + P F + Y S T GV HTT
Sbjct: 282 WWQDVISQYPTSCEVEWVDAEDPLF--LLYTSGSTGKPKGVLHTT 324
>At5g35250.1 68418.m04179 hypothetical protein includes At2g05890,
At4g07450, At3g30630, At3g43100, At2g09960, At3g30550,
At1g39430, At2g10460, At4g03640, At5g35250
Length = 384
Score = 30.3 bits (65), Expect = 1.5
Identities = 16/50 (32%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
Frame = +3
Query: 78 RETLKELGWHLTEEGIDTITENGQIQDVNILSK--KALDYDLRDISEGSL 221
RE +K+ LTE+ + + + +IQD+N +K + +D+ LRD + L
Sbjct: 61 REQIKKFERCLTEDVMGQVVDRSEIQDLNANNKPTEKIDFHLRDQHDNRL 110
>At5g62310.1 68418.m07822 incomplete root hair elongation (IRE) /
protein kinase, putative nearly identical to IRE
(incomplete root hair elongation) [Arabidopsis thaliana]
gi|6729346|dbj|BAA89783
Length = 1168
Score = 29.1 bits (62), Expect = 3.4
Identities = 18/76 (23%), Positives = 36/76 (47%), Gaps = 3/76 (3%)
Frame = +3
Query: 66 NMSLRETLKELGWHLTEEGIDTITENGQ---IQDVNILSKKALDYDLRDISEGSLPEDFV 236
N S +E L E+ +++ +D + + + ++NIL++ ++ +D S GSL
Sbjct: 557 NSSRQEDLDEISQRCSDDMLDCVPRSQNTFSLDELNILNEMSMTNGTKDSSAGSLTPPSP 616
Query: 237 KDPSKLKAPLLFKFRR 284
P + LL R+
Sbjct: 617 ATPRNSQVDLLLSGRK 632
>At5g58840.1 68418.m07373 subtilase family protein contains
similarity to prepro-cucumisin GI:807698 from [Cucumis
melo]; non-consensus acceptor site TT at exon 6
Length = 713
Score = 28.7 bits (61), Expect = 4.5
Identities = 26/89 (29%), Positives = 40/89 (44%), Gaps = 2/89 (2%)
Frame = +2
Query: 254 KSP--VVVQIQKIRNVSAPKANEESTSAPRMLKLTLHDGKVTCTGLEISHIPSLSINTPP 427
KSP V++ + I N +APK S+ P + + + VT G+EI S +N+P
Sbjct: 423 KSPHGTVLKSEAIFNQAAPKVAGFSSRGPNTIAVDILKPDVTAPGVEILAAFS-PLNSPA 481
Query: 428 GTKLLLKNEELEVCHGVVWLTPSVISVWA 514
K ++ + V G P V V A
Sbjct: 482 QDKRDNRHVKYSVLSGTSMSCPHVAGVAA 510
>At5g65310.1 68418.m08216 homeobox-leucine zipper protein 5 (HB-5) /
HD-ZIP transcription factor 5 identical to
homeobox-leucine zipper protein ATHB-5 (HD-ZIP protein
ATHB-5) (SP:P46667) [Arabidopsis thaliana]
Length = 312
Score = 28.3 bits (60), Expect = 5.9
Identities = 19/66 (28%), Positives = 32/66 (48%)
Frame = +3
Query: 21 FNSFSQNNVNLTLNQNMSLRETLKELGWHLTEEGIDTITENGQIQDVNILSKKALDYDLR 200
F++ +N +L N SL +KEL L EG+ I ENG ++ V + ++
Sbjct: 140 FDALKRNRDSLQ-RDNDSLLGQIKELKAKLNVEGVKGIEENGALKAVEANQSVMANNEVL 198
Query: 201 DISEGS 218
++S S
Sbjct: 199 ELSHRS 204
>At3g54520.1 68416.m06033 hypothetical protein
Length = 250
Score = 28.3 bits (60), Expect = 5.9
Identities = 17/58 (29%), Positives = 32/58 (55%), Gaps = 1/58 (1%)
Frame = +3
Query: 9 GLIIFNSFSQNNVNLTLNQNMSL-RETLKELGWHLTEEGIDTITENGQIQDVNILSKK 179
G ++ N SQ+N+ +++ + L + +K+L E GI+T T+ G + + L KK
Sbjct: 167 GELVNNPTSQDNLTISIEMEIKLLTKVIKKLE---RERGIETRTDYGHEEKIKHLEKK 221
>At1g66810.1 68414.m07594 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 310
Score = 28.3 bits (60), Expect = 5.9
Identities = 20/72 (27%), Positives = 29/72 (40%), Gaps = 1/72 (1%)
Frame = -2
Query: 732 LSNSTFSLSLEFWIVLFGSFL*TLELFVHRKSF*SLSKWNPWWRTSFSSYASSCVFGKTS 553
LS+++ S S + +V LEL V+R+ N W T Y +C F
Sbjct: 200 LSSNSVSSSQKVCVVQTKGEREALELEVYRQGMMKTELCNKWQETGACCYGDNCQFAHGI 259
Query: 552 VQF-PFFNHMRY 520
+ P H RY
Sbjct: 260 DELRPVIRHPRY 271
>At5g28750.1 68418.m03531 thylakoid assembly protein, putative
similar to thylakoid assembly 4 (tha4) protein [Pisum
sativum] GI:4929305; contains Pfam profile PF02416:
mttA/Hcf106 family
Length = 147
Score = 27.9 bits (59), Expect = 7.9
Identities = 12/39 (30%), Positives = 24/39 (61%)
Frame = +1
Query: 643 SVDKQFKSLQEAAKQDNPEFEAQRKGAIAEAQRMSGVKK 759
S+ K KS Q+AAK+ E + + + ++AE+ +++ K
Sbjct: 93 SIGKTVKSFQQAAKEFESELKTEPEESVAESSQVATSNK 131
>At5g07770.1 68418.m00889 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 722
Score = 27.9 bits (59), Expect = 7.9
Identities = 11/30 (36%), Positives = 15/30 (50%)
Frame = -3
Query: 623 PNGIHGGGPPSAPMPPLVCLARLLFSSHFS 534
P + GG PP P PP++ +R H S
Sbjct: 642 PPSMSGGAPPPPPPPPMLVASRTAPPPHLS 671
>At3g54670.1 68416.m06049 structural maintenance of chromosomes (SMC)
family protein similar to SMC1 protein [Bos taurus]
GI:4235253, 14S cohesin SMC1 subunit (SMC protein)
[Xenopus laevis] GI:3328231; contains Pfam profiles
PF02483: SMC family C-terminal domain, PF02463:
RecF/RecN/SMC N terminal domain
Length = 1257
Score = 27.9 bits (59), Expect = 7.9
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = +1
Query: 655 QFKSLQEAAKQDNPEFEAQRKGAIAEAQRMSGVKK 759
Q++++QE KQ + EFEA RK A + VK+
Sbjct: 1048 QYEAIQEKEKQVSQEFEAARKEEKQVADAFNTVKQ 1082
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,872,694
Number of Sequences: 28952
Number of extensions: 358457
Number of successful extensions: 1010
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 975
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1010
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1716774400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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