BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021790X (575 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2F7.05c |||translation initiation factor eIF5 |Schizosacchar... 112 3e-26 SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 38 0.001 SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 27 2.0 SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c... 26 3.4 SPAC144.08 |||DNAJ domain protein Jac1 |Schizosaccharomyces pomb... 25 6.0 SPAC12G12.12 |||NST UDP-galactose transporter|Schizosaccharomyce... 25 6.0 SPAC30D11.10 |rad22||DNA repair protein Rad22|Schizosaccharomyce... 25 7.9 >SPAC2F7.05c |||translation initiation factor eIF5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 395 Score = 112 bits (270), Expect = 3e-26 Identities = 51/84 (60%), Positives = 60/84 (71%) Frame = +3 Query: 3 QTQFDFKNERFIVNGSHDSSKLQDLLDGFIRKFVLCPECDNPETELIVSTKRNTISQGCK 182 QT +R+IVNG+HD+ KLQDLLD FIR+FVLC C NPETEL ++ K TIS CK Sbjct: 65 QTTIIADMDRYIVNGAHDAGKLQDLLDVFIRRFVLCASCQNPETELSINKKDQTISYDCK 124 Query: 183 ACGYHGQLDFNHKLNTFILKNPPA 254 ACGY G +D HKL I+KNPPA Sbjct: 125 ACGYRGVIDGRHKLTGVIVKNPPA 148 >SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation factor eIF2 beta subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 321 Score = 37.9 bits (84), Expect = 0.001 Identities = 18/57 (31%), Positives = 32/57 (56%), Gaps = 1/57 (1%) Frame = +3 Query: 24 NERFIVNGSHDSSKLQDLLDGFIRKFVLCPECDNPETELIVSTKRNTI-SQGCKACG 191 + R I+ G +++++L +I ++V C C +P+T + TK N I C+ACG Sbjct: 245 SSRLIIKGRFQQKQIENVLRRYIVEYVTCKTCKSPDT---ILTKENRIFFMTCEACG 298 >SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 617 Score = 27.1 bits (57), Expect = 2.0 Identities = 15/38 (39%), Positives = 18/38 (47%) Frame = -3 Query: 387 SFLASVSCPSWLPLVAVLLDPGPERFERFPRLPSVSEE 274 + LAS W+ L L P PERF P+ S S E Sbjct: 576 TLLASNKVTDWVKLSEFFLGPTPERFTFTPKHKSNSVE 613 >SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 339 Score = 26.2 bits (55), Expect = 3.4 Identities = 8/24 (33%), Positives = 12/24 (50%) Frame = -3 Query: 192 NRRPCILGRWCSASWRQSARSRGC 121 N +P WC + WR+ + GC Sbjct: 167 NEQPAFCVNWCPSRWREQYIAVGC 190 >SPAC144.08 |||DNAJ domain protein Jac1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 225 Score = 25.4 bits (53), Expect = 6.0 Identities = 11/33 (33%), Positives = 18/33 (54%) Frame = -1 Query: 308 SVFHDCPQSVKSLAQPDHGRRVLEYKSIQLVIE 210 S D P+ + L+Q + GR+V E I+ +E Sbjct: 166 SASRDSPEKLLQLSQENQGRKVQEINEIRKAME 198 >SPAC12G12.12 |||NST UDP-galactose transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 324 Score = 25.4 bits (53), Expect = 6.0 Identities = 8/20 (40%), Positives = 12/20 (60%) Frame = +3 Query: 174 GCKACGYHGQLDFNHKLNTF 233 G GYHG D++H ++ F Sbjct: 217 GSTTAGYHGWFDYSHVISRF 236 >SPAC30D11.10 |rad22||DNA repair protein Rad22|Schizosaccharomyces pombe|chr 1|||Manual Length = 469 Score = 25.0 bits (52), Expect = 7.9 Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 4/39 (10%) Frame = +1 Query: 109 ALSATTPRP--S*LSPRSGTPSPK--DARPAVTMDSLTS 213 A++ T+P+ L P +GTPSPK AR A + + S Sbjct: 314 AVTDTSPKTLFDPLKPNTGTPSPKFISARAAAAAEGVVS 352 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,998,721 Number of Sequences: 5004 Number of extensions: 36281 Number of successful extensions: 108 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 105 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 108 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 246098644 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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