BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021787 (661 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ... 124 2e-29 SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu... 114 1e-26 SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ... 99 5e-22 SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit... 95 1e-20 SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 94 1e-20 SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C... 89 4e-19 SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit... 77 3e-15 SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 75 7e-15 SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 38 0.002 SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizo... 29 0.59 SPAC10F6.12c |mam4||protein-S isoprenylcysteine O-methyltransfer... 27 1.8 SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 26 4.2 SPAC821.05 |||translation initiation factor eIF3h|Schizosaccharo... 26 5.5 SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces pomb... 25 7.3 SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 25 9.7 >SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit Cct4|Schizosaccharomyces pombe|chr 2|||Manual Length = 527 Score = 124 bits (298), Expect = 2e-29 Identities = 57/84 (67%), Positives = 72/84 (85%) Frame = +2 Query: 260 GEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEKLLQ 439 GEV +TNDGATILK +SV+HPAAKMLV+LS AQD+EAGDGTTSVV++AG++L AEKLL+ Sbjct: 53 GEVILTNDGATILKHLSVLHPAAKMLVDLSAAQDVEAGDGTTSVVILAGSMLACAEKLLK 112 Query: 440 KGIHPTVISDGFQKALQLALQVVK 511 KGIHPTVI++ FQ+A + +K Sbjct: 113 KGIHPTVIAESFQRAAGFTVDCMK 136 Score = 64.9 bits (151), Expect = 1e-11 Identities = 28/39 (71%), Positives = 36/39 (92%) Frame = +3 Query: 135 YKDKSKPTDIRLSNINAAKAVADAIRTSLGPRGMDKMIQ 251 ++D+ KP ++RLSNI AA++VADAIRTSLGP+GMDKMIQ Sbjct: 11 FQDREKPQEVRLSNIMAARSVADAIRTSLGPKGMDKMIQ 49 Score = 56.4 bits (130), Expect = 3e-09 Identities = 25/45 (55%), Positives = 38/45 (84%) Frame = +1 Query: 526 VDLNNEDALLKAAATSLNSKVVSQHSTILAPIAVQAIRAVMEPIV 660 ++L++ ++LL+AA TSLNSK+VSQ+S +LAPIAV A+ V++P V Sbjct: 142 IELSDRESLLRAATTSLNSKIVSQYSNLLAPIAVDAVLKVIDPRV 186 >SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit Cct5|Schizosaccharomyces pombe|chr 1|||Manual Length = 546 Score = 114 bits (274), Expect = 1e-26 Identities = 53/84 (63%), Positives = 69/84 (82%) Frame = +2 Query: 251 STNGEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEK 430 S +GE+T+TNDGATIL QM V H AK+LV+LS++QD E GDGTT VVV+AGALL+ AE Sbjct: 65 SPDGEITVTNDGATILDQMEVEHQIAKLLVQLSKSQDDEIGDGTTGVVVLAGALLEQAEA 124 Query: 431 LLQKGIHPTVISDGFQKALQLALQ 502 L+ KGIHP I+DG++KA Q+A++ Sbjct: 125 LIDKGIHPIRIADGYEKACQVAVK 148 Score = 39.9 bits (89), Expect = 3e-04 Identities = 17/29 (58%), Positives = 24/29 (82%) Frame = +3 Query: 171 SNINAAKAVADAIRTSLGPRGMDKMIQAP 257 S+I A K VA+ +RTSLGPRG+DK++ +P Sbjct: 38 SHILATKTVANIVRTSLGPRGLDKILISP 66 Score = 31.1 bits (67), Expect = 0.15 Identities = 20/48 (41%), Positives = 29/48 (60%), Gaps = 2/48 (4%) Frame = +1 Query: 514 MSTPVDLNNEDA--LLKAAATSLNSKVVSQHSTILAPIAVQAIRAVME 651 +S VD + E+ L ++A TSL SKVVS+ A IAV A+ +V + Sbjct: 153 ISDVVDFSPENTTNLFRSAKTSLGSKVVSKAHDHFANIAVDAVLSVAD 200 >SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit Cct7|Schizosaccharomyces pombe|chr 2|||Manual Length = 558 Score = 99.1 bits (236), Expect = 5e-22 Identities = 46/84 (54%), Positives = 62/84 (73%) Frame = +2 Query: 260 GEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEKLLQ 439 GEV I+NDGATI+K + ++HPAAK LV+++RAQD E GDGTTSVVV AG LL A ++ Sbjct: 58 GEVVISNDGATIMKLLDIVHPAAKTLVDIARAQDAEVGDGTTSVVVFAGELLREARTFVE 117 Query: 440 KGIHPTVISDGFQKALQLALQVVK 511 G+ +I G++KA QLA+ +K Sbjct: 118 DGVSSHLIIRGYRKAAQLAVNKIK 141 Score = 39.9 bits (89), Expect = 3e-04 Identities = 20/36 (55%), Positives = 24/36 (66%) Frame = +3 Query: 141 DKSKPTDIRLSNINAAKAVADAIRTSLGPRGMDKMI 248 D S+ LSNINA AV D IRT+LGP G DK++ Sbjct: 18 DDSQGRGQLLSNINACVAVQDTIRTTLGPLGADKLM 53 >SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit Cct3|Schizosaccharomyces pombe|chr 2|||Manual Length = 528 Score = 94.7 bits (225), Expect = 1e-20 Identities = 42/83 (50%), Positives = 61/83 (73%) Frame = +2 Query: 260 GEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEKLLQ 439 G V +TNDG IL+++ V HPAAK ++EL+R QD E GDGTTSV+++AG +L +A LL Sbjct: 53 GSVLLTNDGHAILREIEVAHPAAKSMIELARTQDEEVGDGTTSVIILAGEILAAASPLLD 112 Query: 440 KGIHPTVISDGFQKALQLALQVV 508 + IHP V+ F++AL+ AL ++ Sbjct: 113 RKIHPVVMIRSFKQALEDALSII 135 Score = 44.4 bits (100), Expect = 1e-05 Identities = 21/32 (65%), Positives = 25/32 (78%) Frame = +3 Query: 165 RLSNINAAKAVADAIRTSLGPRGMDKMIQAPM 260 ++SNI AAKAVAD IRT LGPR M KM+ P+ Sbjct: 21 QMSNIQAAKAVADVIRTCLGPRAMLKMLLDPV 52 Score = 28.3 bits (60), Expect = 1.0 Identities = 10/44 (22%), Positives = 29/44 (65%) Frame = +1 Query: 514 MSTPVDLNNEDALLKAAATSLNSKVVSQHSTILAPIAVQAIRAV 645 ++ PV++++ + + T + +K+V++ S ++ +A++A+R V Sbjct: 138 ITLPVNVDDNAEMFRLIRTCIGTKLVARWSDLMCHLALRAVRTV 181 >SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit Cct1|Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 94.3 bits (224), Expect = 1e-20 Identities = 45/81 (55%), Positives = 61/81 (75%) Frame = +2 Query: 260 GEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEKLLQ 439 G+VT+TNDGATIL + V HPA K+LVEL++ QD E GDGTTSVV+IA LL A +L++ Sbjct: 55 GDVTVTNDGATILSLLDVEHPAGKVLVELAQQQDKEVGDGTTSVVIIAAELLRRANELVK 114 Query: 440 KGIHPTVISDGFQKALQLALQ 502 IHPT I G++ A++ A++ Sbjct: 115 NKIHPTTIITGYRLAIREAVK 135 Score = 37.9 bits (84), Expect = 0.001 Identities = 15/36 (41%), Positives = 25/36 (69%) Frame = +3 Query: 141 DKSKPTDIRLSNINAAKAVADAIRTSLGPRGMDKMI 248 +K D+R N+ A A+A+ +++SLGP G+DKM+ Sbjct: 15 EKISGEDVRNQNVLATTAIANVVKSSLGPVGLDKML 50 >SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit Cct2|Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 89.4 bits (212), Expect = 4e-19 Identities = 42/88 (47%), Positives = 63/88 (71%) Frame = +2 Query: 248 SSTNGEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAE 427 S+++G++ +TNDGATILK +++ + AAK+LV +S+ QD E GDGTTSV V A LL AE Sbjct: 51 SNSSGDIVVTNDGATILKSIALDNAAAKVLVNISKVQDDEVGDGTTSVCVFAAELLRQAE 110 Query: 428 KLLQKGIHPTVISDGFQKALQLALQVVK 511 ++ IHP VI DG++ A + A+ ++ Sbjct: 111 IMVNAKIHPQVIIDGYRIATKTAIDALR 138 Score = 37.1 bits (82), Expect = 0.002 Identities = 15/30 (50%), Positives = 24/30 (80%) Frame = +3 Query: 165 RLSNINAAKAVADAIRTSLGPRGMDKMIQA 254 RLS+ A AV D ++++LGP+GMDK++Q+ Sbjct: 22 RLSSFVGAIAVGDLVKSTLGPKGMDKILQS 51 >SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit Cct8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 546 Score = 76.6 bits (180), Expect = 3e-15 Identities = 35/83 (42%), Positives = 57/83 (68%), Gaps = 1/83 (1%) Frame = +2 Query: 272 ITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEKLLQKGIH 451 +TND ATI++++ VIHPAAK++V+ ++ Q+ E GD VVV G LL AE +++ G+ Sbjct: 64 LTNDAATIIRELEVIHPAAKLVVDATQQQENELGDAANFVVVFTGELLAKAENMIRMGLT 123 Query: 452 PTVISDGFQKALQLALQVV-KIC 517 P I+ G++ AL ++V+ +IC Sbjct: 124 PLEIAKGYEMALSHTMEVLEEIC 146 Score = 33.1 bits (72), Expect = 0.036 Identities = 17/55 (30%), Positives = 28/55 (50%) Frame = +3 Query: 84 IMAPKAGGDAIKANSSVYKDKSKPTDIRLSNINAAKAVADAIRTSLGPRGMDKMI 248 + PKA G + Y+ D + N NA + +++ RTSLGP G +K++ Sbjct: 3 LRVPKASGPQLFREG--YRIMQGVEDAVIRNCNAIRELSEITRTSLGPNGKNKIV 55 >SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit Cct6|Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 75.4 bits (177), Expect = 7e-15 Identities = 34/80 (42%), Positives = 51/80 (63%) Frame = +2 Query: 260 GEVTITNDGATILKQMSVIHPAAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAEKLLQ 439 G + +T DG +L +M + +P A + + + AQD GDGTTSV ++ G LL AE ++ Sbjct: 50 GAIKLTKDGKVLLTEMQIQNPTASCIAKAATAQDDATGDGTTSVCLLVGELLKQAELYIR 109 Query: 440 KGIHPTVISDGFQKALQLAL 499 +G+HP++ISDGF A AL Sbjct: 110 EGLHPSLISDGFNLAKNEAL 129 Score = 30.3 bits (65), Expect = 0.26 Identities = 15/39 (38%), Positives = 22/39 (56%) Frame = +1 Query: 538 NEDALLKAAATSLNSKVVSQHSTILAPIAVQAIRAVMEP 654 + + LL A TSL++K+ S+ LAP V AI + P Sbjct: 142 DREVLLNVAKTSLSTKISSKVVESLAPAVVDAILTIRRP 180 Score = 28.3 bits (60), Expect = 1.0 Identities = 11/25 (44%), Positives = 18/25 (72%) Frame = +3 Query: 174 NINAAKAVADAIRTSLGPRGMDKMI 248 NI+AA + D ++++LGP G KM+ Sbjct: 21 NISAAIGLQDVLKSNLGPTGTTKML 45 >SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60|Schizosaccharomyces pombe|chr 1|||Manual Length = 582 Score = 37.5 bits (83), Expect = 0.002 Identities = 21/88 (23%), Positives = 44/88 (50%), Gaps = 4/88 (4%) Frame = +2 Query: 260 GEVTITNDGATILKQMSVIHP----AAKMLVELSRAQDIEAGDGTTSVVVIAGALLDSAE 427 G IT DG T+ + +S+ A+++ +++ + AGDGTT+ V+ A+ Sbjct: 76 GSPKITKDGVTVARSVSLKDKFENLGARLVQDVASKTNEVAGDGTTTATVLTRAIFSETV 135 Query: 428 KLLQKGIHPTVISDGFQKALQLALQVVK 511 + + G +P + G Q A+ ++ ++ Sbjct: 136 RNVAAGCNPMDLRRGIQLAVDNVVEFLQ 163 >SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizosaccharomyces pombe|chr 2|||Manual Length = 551 Score = 29.1 bits (62), Expect = 0.59 Identities = 14/26 (53%), Positives = 14/26 (53%) Frame = +3 Query: 564 CNITKFQSSFTTLNYFGTHCSASNSS 641 CNI KF SSF T N F T SS Sbjct: 276 CNIPKFNSSFKTSNDFFTFTKTEESS 301 >SPAC10F6.12c |mam4||protein-S isoprenylcysteine O-methyltransferase Mam4|Schizosaccharomyces pombe|chr 1|||Manual Length = 236 Score = 27.5 bits (58), Expect = 1.8 Identities = 14/38 (36%), Positives = 21/38 (55%) Frame = +1 Query: 307 ECYSSSCKNVGRIISCSRY*SW*WNNISSCYCWCVVGF 420 EC S K+ ++I+C R+ S+ N I S Y +GF Sbjct: 87 ECLLSGGKSFAKVINCLRFPSFLINFIFSVYQTSALGF 124 >SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2670 Score = 26.2 bits (55), Expect = 4.2 Identities = 13/47 (27%), Positives = 22/47 (46%) Frame = +1 Query: 517 STPVDLNNEDALLKAAATSLNSKVVSQHSTILAPIAVQAIRAVMEPI 657 ST D L+ + N ++ H +ILAP +RA++ P+ Sbjct: 2537 STSQDSRRLALLIIRVVSKENYSLIKPHISILAPAIFGCVRAIVIPV 2583 >SPAC821.05 |||translation initiation factor eIF3h|Schizosaccharomyces pombe|chr 1|||Manual Length = 357 Score = 25.8 bits (54), Expect = 5.5 Identities = 12/25 (48%), Positives = 16/25 (64%) Frame = -2 Query: 258 LVLESSYPFHEALSLCGWHQQQLLR 184 L+LES+ FH G+HQ+QL R Sbjct: 253 LLLESTDEFHYEQGNLGFHQRQLAR 277 >SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 542 Score = 25.4 bits (53), Expect = 7.3 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -1 Query: 427 LSRIQQRTSNNNY*CCSITSFNI 359 LS Q TS NN+ C SI +F I Sbjct: 177 LSNRQLNTSENNWTCLSIENFGI 199 >SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated membrane proteins, ESCRT 0 complex|Schizosaccharomyces pombe|chr 1|||Manual Length = 610 Score = 25.0 bits (52), Expect = 9.7 Identities = 11/29 (37%), Positives = 16/29 (55%), Gaps = 2/29 (6%) Frame = -2 Query: 657 NRFHYCSNC--LHCNGCQNS*VL*NYFGI 577 NR H+C NC + CN C + + + GI Sbjct: 187 NRKHHCRNCGGVFCNQCSSKTLSLPHLGI 215 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,530,590 Number of Sequences: 5004 Number of extensions: 48574 Number of successful extensions: 158 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 142 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 158 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 299817502 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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