Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021784
(686 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 105 4e-23
At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 102 3e-22
At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 101 3e-22
At3g06450.1 68416.m00746 anion exchange family protein similar t... 31 0.95
At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 29 2.9
At5g41460.1 68418.m05035 fringe-related protein strong similarit... 29 3.8
At5g13880.1 68418.m01622 expressed protein 29 3.8
At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 3.8
At2g27680.1 68415.m03354 aldo/keto reductase family protein cont... 28 5.0
At3g53330.1 68416.m05884 plastocyanin-like domain-containing pro... 28 6.7
At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein... 28 6.7
At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-... 27 8.8
At3g59820.1 68416.m06675 calcium-binding mitochondrial protein-r... 27 8.8
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 27 8.8
At1g20860.1 68414.m02613 phosphate transporter family protein si... 27 8.8
>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Arabidopsis thaliana}
Length = 158
Score = 105 bits (251), Expect = 4e-23
Identities = 46/83 (55%), Positives = 63/83 (75%), Gaps = 1/83 (1%)
Frame = +3
Query: 255 FNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LGT 431
F KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L
Sbjct: 65 FTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQ 124
Query: 432 QLRTDFDSGKELLCTVLKSCGEE 500
Q+++ FD GK+L+ +V+ + GEE
Sbjct: 125 QIKSGFDDGKDLVVSVMSAMGEE 147
Score = 101 bits (241), Expect = 6e-22
Identities = 42/72 (58%), Positives = 55/72 (76%)
Frame = +1
Query: 73 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 252
E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 253 ISMVKSMKISVP 288
I K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similarity to
eukaryotic initiation factor 5A (2) (Nicotiana
plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Lycopersicon esculentum}
Length = 158
Score = 102 bits (244), Expect = 3e-22
Identities = 46/90 (51%), Positives = 65/90 (72%), Gaps = 1/90 (1%)
Frame = +3
Query: 255 FNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGT 431
F KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L T
Sbjct: 65 FTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLT 124
Query: 432 QLRTDFDSGKELLCTVLKSCGEECVMPSKQ 521
QL+ F+ GK+++ +V+ + GEE + K+
Sbjct: 125 QLKNGFEEGKDIVVSVMSAMGEEQMCALKE 154
Score = 97.5 bits (232), Expect = 7e-21
Identities = 41/72 (56%), Positives = 54/72 (75%)
Frame = +1
Query: 73 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 252
++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 253 ISMVKSMKISVP 288
I K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similariy to
SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
(eIF-5A 1) {Lycopersicon esculentum}
Length = 159
Score = 101 bits (243), Expect = 3e-22
Identities = 45/90 (50%), Positives = 64/90 (71%), Gaps = 1/90 (1%)
Frame = +3
Query: 255 FNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGT 431
F KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L
Sbjct: 65 FTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTA 124
Query: 432 QLRTDFDSGKELLCTVLKSCGEECVMPSKQ 521
Q+R FD GK+++ +V+ S GEE + K+
Sbjct: 125 QMRLGFDEGKDIVVSVMSSMGEEQICAVKE 154
Score = 93.1 bits (221), Expect = 2e-19
Identities = 39/72 (54%), Positives = 51/72 (70%)
Frame = +1
Query: 73 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 252
++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 253 ISMVKSMKISVP 288
I K ++ VP
Sbjct: 64 IFTAKKLEDIVP 75
>At3g06450.1 68416.m00746 anion exchange family protein similar to
putative Anion exchanger family members: GB:AAD39673,
GB:AAD55295 [Arabidopsis thaliana]
Length = 732
Score = 30.7 bits (66), Expect = 0.95
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = -3
Query: 675 LRAPPLYYYSFFKLNLYTILC 613
LR PP Y+Y F L TILC
Sbjct: 328 LRKPPAYHYDLFLLGFLTILC 348
>At5g14230.1 68418.m01663 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 591
Score = 29.1 bits (62), Expect = 2.9
Identities = 15/34 (44%), Positives = 16/34 (47%)
Frame = +3
Query: 402 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 503
L IPDGD T L G +C L SCG C
Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445
>At5g41460.1 68418.m05035 fringe-related protein strong similarity
to unknown protein (pir||T13026) similarity to predicted
proteins + similar to hypothetical protein GB:AAC23643
[Arabidopsis thaliana] + weak similarity to Fringe
[Schistocerca gregaria](GI:6573138);Fringe encodes an
extracellular protein that regulates Notch signalling.
Length = 524
Score = 28.7 bits (61), Expect = 3.8
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = +2
Query: 38 VVKFKTQQWVTSKTHTSRPETPGPQPPSP 124
++ F T Q K++ S P +P P PP P
Sbjct: 80 LILFHTNQTAVIKSYASPPPSPPPPPPPP 108
>At5g13880.1 68418.m01622 expressed protein
Length = 188
Score = 28.7 bits (61), Expect = 3.8
Identities = 9/36 (25%), Positives = 19/36 (52%)
Frame = +2
Query: 2 VEEPVGHISFLTVVKFKTQQWVTSKTHTSRPETPGP 109
+E+ + ++ L + +F Q W+ + P+TP P
Sbjct: 150 LEDEIDKVADLFITRFHRQMWLQKQLSLESPDTPSP 185
>At5g03140.1 68418.m00262 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 711
Score = 28.7 bits (61), Expect = 3.8
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -1
Query: 173 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 66
+P +T+P +R + A+ PE P++K SSMS
Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666
>At2g27680.1 68415.m03354 aldo/keto reductase family protein
contains Pfam profile PF00248: oxidoreductase, aldo/keto
reductase family
Length = 384
Score = 28.3 bits (60), Expect = 5.0
Identities = 26/95 (27%), Positives = 44/95 (46%)
Frame = +3
Query: 273 EDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRTDFD 452
++I S MDV + + D ++DGYL + DL+E +G + T T+FD
Sbjct: 147 QNIDISRKRMDVAALDMLQFHWWDYANDGYLDALKHLTDLKE-----EGKIKTVALTNFD 201
Query: 453 SGKELLCTVLKSCGEECVMPSKQTQLSTNKPTQHL 557
+ E L +L++ G V Q + +P Q +
Sbjct: 202 T--ERLQKILEN-GIPVVSNQVQHSIVDMRPQQRM 233
>At3g53330.1 68416.m05884 plastocyanin-like domain-containing
protein similar to mavicyanin SP:P80728 from [Cucurbita
pepo]
Length = 310
Score = 27.9 bits (59), Expect = 6.7
Identities = 13/18 (72%), Positives = 13/18 (72%), Gaps = 1/18 (5%)
Frame = +2
Query: 71 SKTHT-SRPETPGPQPPS 121
SKTH SRP TP P PPS
Sbjct: 127 SKTHERSRPITPSPPPPS 144
>At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein
contains INTERPRO domain, IPR001878: Zn-finger CCHC type
Length = 353
Score = 27.9 bits (59), Expect = 6.7
Identities = 15/45 (33%), Positives = 17/45 (37%), Gaps = 3/45 (6%)
Frame = +2
Query: 74 KTHTSRPETPGPQPPS---PCNVRPCVKTVSLC*RVVHARLLKCP 199
KT T P PP PCN PC S + + R CP
Sbjct: 22 KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66
>At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box
RNA helicase [Chlamydomonas reinhardtii] GI:12044832;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1226
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = +3
Query: 315 VKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDL-GTQLRTDFDSGKE 464
V+R+ +++ Y L ADN + + G + GT+++T+FDS +E
Sbjct: 350 VRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEEE 400
>At3g59820.1 68416.m06675 calcium-binding mitochondrial
protein-related contains weak similarity to
Calcium-binding mitochondrial protein Anon-60Da
(Swiss-Prot:P91927) [Drosophila melanogaster]
Length = 755
Score = 27.5 bits (58), Expect = 8.8
Identities = 16/44 (36%), Positives = 21/44 (47%)
Frame = +2
Query: 131 VRPCVKTVSLC*RVVHARLLKCPHPKPESTATLKFTWLGLIFQW 262
+ P +K V+ R A+ K H K E +TLK WLG W
Sbjct: 182 IGPAIKAVASMNRADWAK--KLTHWKHEFVSTLKHYWLGTKLLW 223
>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to
SP|P35207 Antiviral protein SKI2 {Saccharomyces
cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain
Length = 1347
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 6/51 (11%)
Frame = +3
Query: 345 ISDDGYLTLMADNG--DLREDLKIPDGDLGTQLR----TDFDSGKELLCTV 479
+SD+ L + A G D+ +++ D DL Q++ + +SG+EL+CTV
Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191
>At1g20860.1 68414.m02613 phosphate transporter family protein
similar to phosphate transporter [Catharanthus roseus]
GI:2208908, inorganic phosphate transporter 1 [Solanum
tuberosum] GI:1420871; contains Pfam profile PF00083:
major facilitator superfamily protein
Length = 534
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/45 (37%), Positives = 20/45 (44%)
Frame = +2
Query: 50 KTQQWVTSKTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHAR 184
K Q V S++H S T P PP P P K S C +H R
Sbjct: 243 KDMQRVMSRSHISDEATTDPPPPPP---PPSYKLFSRCFFRLHGR 284
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,831,552
Number of Sequences: 28952
Number of extensions: 356319
Number of successful extensions: 1078
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 989
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1064
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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