Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021765
(721 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g12460.1 68417.m01971 oxysterol-binding family protein simila... 42 3e-04
At1g77730.1 68414.m09050 pleckstrin homology (PH) domain-contain... 42 3e-04
At4g22540.1 68417.m03253 oxysterol-binding family protein simila... 42 5e-04
At2g31020.1 68415.m03782 oxysterol-binding family protein simila... 41 0.001
At1g13170.1 68414.m01527 oxysterol-binding family protein simila... 40 0.001
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 40 0.002
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 40 0.002
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 40 0.002
At2g30880.2 68415.m03764 pleckstrin homology (PH) domain-contain... 37 0.015
At2g30880.1 68415.m03763 pleckstrin homology (PH) domain-contain... 37 0.015
At1g59610.1 68414.m06704 dynamin-like protein, putative (ADL3) s... 33 0.19
At1g10290.1 68414.m01159 dynamin-like protein 6 (ADL6) identical... 33 0.19
At4g34170.1 68417.m04848 kelch repeat-containing F-box family pr... 30 1.3
At4g19040.1 68417.m02805 pleckstrin homology (PH) domain-contain... 30 1.8
At3g02820.1 68416.m00274 zinc knuckle (CCHC-type) family protein... 29 4.1
At5g45560.1 68418.m05595 pleckstrin homology (PH) domain-contain... 28 5.4
At1g64790.1 68414.m07346 translational activator family protein ... 27 9.5
>At4g12460.1 68417.m01971 oxysterol-binding family protein similar
to SP|P22059 Oxysterol-binding protein 1 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 694
Score = 42.3 bits (95), Expect = 3e-04
Identities = 24/58 (41%), Positives = 31/58 (53%)
Frame = +3
Query: 138 LTSHRPLSGQLYKYTNVVKGWQQRWFAVDPETGVLSYYLMMDQETQYSLDSRPGGRLI 311
L S R ++G LYK+TN KGW+ RWF + G+LSY + E L RLI
Sbjct: 22 LQSGRSVAGILYKWTNYGKGWRSRWFLL--RDGILSYSKIRRPENVNLLSPSDDLRLI 77
>At1g77730.1 68414.m09050 pleckstrin homology (PH) domain-containing
protein similar to SP|P16258 Oxysterol-binding protein 1
{Oryctolagus cuniculus}; contains Pfam profile PF00169:
PH domain
Length = 265
Score = 42.3 bits (95), Expect = 3e-04
Identities = 23/63 (36%), Positives = 33/63 (52%)
Frame = +2
Query: 266 GDTIQPGQPARGEAHLAAAVICPSDEDSKTFTINCASGDMLKLRATDARARQEWVDGLRA 445
GD PG+P G+ HL + I S D K FT+ + L LRA + R W++ L+A
Sbjct: 119 GDVHSPGKPL-GQIHLKVSSIGQSISDGKRFTVFTGTKS-LHLRAATSEDRASWIEALKA 176
Query: 446 IAE 454
+ E
Sbjct: 177 VKE 179
Score = 39.5 bits (88), Expect = 0.002
Identities = 16/32 (50%), Positives = 24/32 (75%)
Frame = +3
Query: 156 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSYY 251
++G LYK+ N +GW++RWF + + GVLSYY
Sbjct: 54 VAGILYKWVNYGQGWKRRWFVL--QDGVLSYY 83
>At4g22540.1 68417.m03253 oxysterol-binding family protein similar
to SP|P16258 Oxysterol-binding protein 1 {Oryctolagus
cuniculus}; contains Pfam profiles PF00169: PH domain,
PF01237: Oxysterol-binding protein
Length = 721
Score = 41.5 bits (93), Expect = 5e-04
Identities = 23/60 (38%), Positives = 31/60 (51%)
Frame = +3
Query: 132 TGLTSHRPLSGQLYKYTNVVKGWQQRWFAVDPETGVLSYYLMMDQETQYSLDSRPGGRLI 311
TG + ++G LYK+TN KGW+ RWF + G+LSY + E L RLI
Sbjct: 45 TGGSESETVAGILYKWTNFGKGWRSRWFLL--RNGILSYSKIRRPENLNLLSPDDDVRLI 102
>At2g31020.1 68415.m03782 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 760
Score = 40.7 bits (91), Expect = 0.001
Identities = 17/32 (53%), Positives = 23/32 (71%)
Frame = +3
Query: 156 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSYY 251
++G LYK+ N KGW+ RWF + + GVLSYY
Sbjct: 73 IAGILYKWVNYGKGWRPRWFVL--QDGVLSYY 102
Score = 34.3 bits (75), Expect = 0.082
Identities = 18/53 (33%), Positives = 28/53 (52%)
Frame = +2
Query: 299 GEAHLAAAVICPSDEDSKTFTINCASGDMLKLRATDARARQEWVDGLRAIAEI 457
GE HL + I S D K F+I + L LRA R+ W++ L+A+ ++
Sbjct: 153 GEVHLKVSSIRESRSDDKRFSIFTGT-KRLHLRAETREDREAWIEALQAVKDM 204
>At1g13170.1 68414.m01527 oxysterol-binding family protein similar
to SP|P16258 Oxysterol-binding protein 1 {Oryctolagus
cuniculus}; contains Pfam profiles PF00169: PH domain,
PF01237: Oxysterol-binding protein
Length = 816
Score = 40.3 bits (90), Expect = 0.001
Identities = 17/32 (53%), Positives = 23/32 (71%)
Frame = +3
Query: 156 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSYY 251
++G +YK+ N KGW+ RWF + E GVLSYY
Sbjct: 95 VAGIMYKWVNYGKGWRARWFEL--EDGVLSYY 124
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 39.9 bits (89), Expect = 0.002
Identities = 19/53 (35%), Positives = 33/53 (62%), Gaps = 1/53 (1%)
Frame = +3
Query: 156 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSYYLMMDQETQY-SLDSRPGGRLI 311
++G LYK+ N +GW+ RWF + + GVLSYY + + + S ++ G ++I
Sbjct: 106 IAGILYKWVNYGRGWRPRWFVL--QDGVLSYYKIHGPDKIFVSPETEKGSKVI 156
Score = 35.1 bits (77), Expect = 0.047
Identities = 24/87 (27%), Positives = 39/87 (44%)
Frame = +2
Query: 260 GPGDTIQPGQPARGEAHLAAAVICPSDEDSKTFTINCASGDMLKLRATDARARQEWVDGL 439
G + Q + GE HL + + S D K F+I + L LRA R WV+ L
Sbjct: 172 GSSSSCQLRRKPFGEVHLKVSSVRESRSDDKRFSIFTGT-KRLHLRAETREDRTTWVEAL 230
Query: 440 RAIAEIHTKAMGANPPLQPREQLRSTT 520
+A+ ++ + M + + P L +T
Sbjct: 231 QAVKDMFPR-MSNSELMAPTNNLAMST 256
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 39.9 bits (89), Expect = 0.002
Identities = 19/53 (35%), Positives = 33/53 (62%), Gaps = 1/53 (1%)
Frame = +3
Query: 156 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSYYLMMDQETQY-SLDSRPGGRLI 311
++G LYK+ N +GW+ RWF + + GVLSYY + + + S ++ G ++I
Sbjct: 106 IAGILYKWVNYGRGWRPRWFVL--QDGVLSYYKIHGPDKIFVSPETEKGSKVI 156
Score = 35.1 bits (77), Expect = 0.047
Identities = 24/87 (27%), Positives = 39/87 (44%)
Frame = +2
Query: 260 GPGDTIQPGQPARGEAHLAAAVICPSDEDSKTFTINCASGDMLKLRATDARARQEWVDGL 439
G + Q + GE HL + + S D K F+I + L LRA R WV+ L
Sbjct: 172 GSSSSCQLRRKPFGEVHLKVSSVRESRSDDKRFSIFTGT-KRLHLRAETREDRTTWVEAL 230
Query: 440 RAIAEIHTKAMGANPPLQPREQLRSTT 520
+A+ ++ + M + + P L +T
Sbjct: 231 QAVKDMFPR-MSNSELMAPTNNLAMST 256
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 39.9 bits (89), Expect = 0.002
Identities = 19/53 (35%), Positives = 33/53 (62%), Gaps = 1/53 (1%)
Frame = +3
Query: 156 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSYYLMMDQETQY-SLDSRPGGRLI 311
++G LYK+ N +GW+ RWF + + GVLSYY + + + S ++ G ++I
Sbjct: 106 IAGILYKWVNYGRGWRPRWFVL--QDGVLSYYKIHGPDKIFVSPETEKGSKVI 156
Score = 35.1 bits (77), Expect = 0.047
Identities = 24/87 (27%), Positives = 39/87 (44%)
Frame = +2
Query: 260 GPGDTIQPGQPARGEAHLAAAVICPSDEDSKTFTINCASGDMLKLRATDARARQEWVDGL 439
G + Q + GE HL + + S D K F+I + L LRA R WV+ L
Sbjct: 172 GSSSSCQLRRKPFGEVHLKVSSVRESRSDDKRFSIFTGT-KRLHLRAETREDRTTWVEAL 230
Query: 440 RAIAEIHTKAMGANPPLQPREQLRSTT 520
+A+ ++ + M + + P L +T
Sbjct: 231 QAVKDMFPR-MSNSELMAPTNNLAMST 256
>At2g30880.2 68415.m03764 pleckstrin homology (PH) domain-containing
protein contains Pfam profile PF00169: PH domain
Length = 381
Score = 36.7 bits (81), Expect = 0.015
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = +3
Query: 156 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSYYLMMDQET 272
L G L+K + ++ W +RW +DP TG + Y ++ T
Sbjct: 34 LQGPLFKRSETLRKWNERWVILDPTTGKMEYKTRRNEPT 72
>At2g30880.1 68415.m03763 pleckstrin homology (PH) domain-containing
protein contains Pfam profile PF00169: PH domain
Length = 504
Score = 36.7 bits (81), Expect = 0.015
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = +3
Query: 156 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSYYLMMDQET 272
L G L+K + ++ W +RW +DP TG + Y ++ T
Sbjct: 34 LQGPLFKRSETLRKWNERWVILDPTTGKMEYKTRRNEPT 72
>At1g59610.1 68414.m06704 dynamin-like protein, putative (ADL3)
strong similarity to dynamin-like protein 6 (ADL6)
[Arabidopsis thaliana] GI:6651399; contains Pfam
profiles PF01031: Dynamin central region, PF00350:
Dynamin family, PF02212: Dynamin GTPase effector domain,
PF00169: PH domain; identical to cDNA dynamin-like
protein ADL3, GI:4803835
Length = 920
Score = 33.1 bits (72), Expect = 0.19
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +3
Query: 126 MKTGLTSHRPLSGQLYKYTNVVKGWQQRWFAVDPETGVLSY 248
+KT +G L K + GW +RWF ++ +TG L Y
Sbjct: 572 LKTAGPEGEITAGYLMKKSAKTNGWSRRWFVLNEKTGKLGY 612
>At1g10290.1 68414.m01159 dynamin-like protein 6 (ADL6) identical to
dynamin-like protein 6 (ADL6) [Arabidopsis thaliana]
GI:6651399; contains Pfam profiles PF01031: Dynamin
central region, PF00350: Dynamin family, PF02212:
Dynamin GTPase effector domain, PF00169: PH domain
Length = 914
Score = 33.1 bits (72), Expect = 0.19
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +3
Query: 126 MKTGLTSHRPLSGQLYKYTNVVKGWQQRWFAVDPETGVLSY 248
+KT +G L K + GW +RWF ++ +TG L Y
Sbjct: 565 LKTAGPEGEITAGYLMKKSAKTNGWSRRWFVLNEKTGKLGY 605
>At4g34170.1 68417.m04848 kelch repeat-containing F-box family
protein low similarity to SKP1 interacting partner 6
[Arabidopsis thaliana] GI:10716957; contains Pfam
profiles PF01344: Kelch motif, PF00646: F-box domain
Length = 293
Score = 30.3 bits (65), Expect = 1.3
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = -3
Query: 515 WTGAALSAAGAGWPPSPWCV 456
W+ A +SA G GWP S +CV
Sbjct: 210 WSAADMSANGWGWPGSSYCV 229
>At4g19040.1 68417.m02805 pleckstrin homology (PH) domain-containing
protein / lipid-binding START domain-containing protein
contains Pfam profiles PF01852: START domain, PF00169:
PH domain
Length = 718
Score = 29.9 bits (64), Expect = 1.8
Identities = 16/52 (30%), Positives = 25/52 (48%)
Frame = -1
Query: 475 PHRLGVYLSNGPEPVDPLLARAGVGSSQLQHVAGRAVYGEGLGVFIAWAYHC 320
P + +L +G + PL R G +Q+QH+ + G G G A+ HC
Sbjct: 324 PGCVRAHLESGGYNISPLKPRNGRPRTQVQHLIQIDLKGWGAGYLPAFQQHC 375
>At3g02820.1 68416.m00274 zinc knuckle (CCHC-type) family protein
contains Pfam domain, PF00098: Zinc knuckle
Length = 282
Score = 28.7 bits (61), Expect = 4.1
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -3
Query: 704 CWRCSTPRHCSRHCMLAADVAFRSNKSGS 618
C++C P H SR C +A VA ++ S S
Sbjct: 8 CFKCGRPGHWSRDCPSSAPVAGNNSVSSS 36
>At5g45560.1 68418.m05595 pleckstrin homology (PH) domain-containing
protein / lipid-binding START domain-containing protein
weak similarity to SP|P79245 Steroidogenic acute
regulatory protein, mitochondrial precursor (StAR) {Ovis
aries}; contains Pfam profiles PF01852: START domain,
PF00169: PH domain
Length = 719
Score = 28.3 bits (60), Expect = 5.4
Identities = 16/52 (30%), Positives = 25/52 (48%)
Frame = -1
Query: 475 PHRLGVYLSNGPEPVDPLLARAGVGSSQLQHVAGRAVYGEGLGVFIAWAYHC 320
P + +L +G + PL R G +Q+QH+ + G G G A+ HC
Sbjct: 324 PGFVRAHLESGGFNIAPLKPRNGRPRTQVQHLIQIDLKGWGSGYLPAFQQHC 375
>At1g64790.1 68414.m07346 translational activator family protein
similar to HsGCN1 [Homo sapiens] GI:2282576
Length = 2440
Score = 27.5 bits (58), Expect = 9.5
Identities = 16/56 (28%), Positives = 26/56 (46%)
Frame = -2
Query: 462 VCISAMALSPSTHSWRARASVALSFNMSPDAQFMVKVLESSSLGHITAAAK*ASPL 295
+C +A SW +R+SVA+ N+ +K E+ GH+ + SPL
Sbjct: 294 ICSFLIACYKDEASWASRSSVAIQPNLVSFIAAGLKEKEALRRGHLRCISDLLSPL 349
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,884,089
Number of Sequences: 28952
Number of extensions: 313459
Number of successful extensions: 1012
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 973
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1012
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1565336320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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