Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021755
(712 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55360.1 68416.m06148 3-oxo-5-alpha-steroid 4-dehydrogenase f... 87 8e-18
At3g57940.1 68416.m06458 expressed protein contains Pfam profile... 31 0.75
At5g60800.1 68418.m07628 heavy-metal-associated domain-containin... 29 3.0
At5g16010.1 68418.m01872 3-oxo-5-alpha-steroid 4-dehydrogenase f... 29 3.0
At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyu... 29 3.0
At5g36740.1 68418.m04402 PHD finger family protein 29 4.0
At5g36670.1 68418.m04388 PHD finger family protein 29 4.0
At5g06930.1 68418.m00783 expressed protein 28 5.3
At3g18080.1 68416.m02299 glycosyl hydrolase family 1 protein con... 28 5.3
At1g10490.1 68414.m01181 expressed protein contains Pfam profile... 28 5.3
At3g26490.1 68416.m03304 phototropic-responsive NPH3 family prot... 28 7.0
At3g18200.1 68416.m02315 nodulin MtN21 family protein similar to... 28 7.0
At2g20142.1 68415.m02355 expressed protein 28 7.0
At5g41610.2 68418.m05055 cation/hydrogen exchanger, putative (CH... 27 9.3
At5g41610.1 68418.m05056 cation/hydrogen exchanger, putative (CH... 27 9.3
At3g57060.1 68416.m06353 non-SMC condensin subunit, XCAP-D2/Cnd1... 27 9.3
At2g04430.1 68415.m00447 MutT/nudix family protein similar to SP... 27 9.3
>At3g55360.1 68416.m06148 3-oxo-5-alpha-steroid 4-dehydrogenase
family protein / steroid 5-alpha-reductase family
protein similar to synaptic glycoprotein SC2 spliced
variant from Homo sapiens [EMBL:AF038958], SC2 from
Rattus sp. [gi:256994]; contains Pfam
3-oxo-5-alpha-steroid 4-dehydrogenase domain PF02544
Length = 310
Score = 87.4 bits (207), Expect = 8e-18
Identities = 39/72 (54%), Positives = 47/72 (65%)
Frame = +2
Query: 488 FVHSSLFHGTMPLKNLFRNCAYYWLFALYVAYHVNHPLYTAPCTTCYYIGLGGFVLCELG 667
FVH H T P+ N+FRNCAYYW F Y+AY+VNHPLYT IG G ++C++
Sbjct: 151 FVHR-FSHATSPIGNVFRNCAYYWSFGAYIAYYVNHPLYTPVSDLQMKIGFGFGLVCQVA 209
Query: 668 NLSIHILLKNLR 703
N HILLKNLR
Sbjct: 210 NFYCHILLKNLR 221
Score = 64.5 bits (150), Expect = 7e-11
Identities = 44/128 (34%), Positives = 65/128 (50%), Gaps = 5/128 (3%)
Frame = +3
Query: 141 FDKECKRKNPTEREEISIPRSSGPQTGG*RQNVER*--RYLNSLNIQDGSKLFLKDLGPQ 314
F K K+ P+ R+ +++P + G + N ++ Y + N + + KDLG Q
Sbjct: 33 FHKRAKKFYPS-RQRLTLPVTPGSKDKPVVLNSKKSLKEYCDGNN--NSLTVVFKDLGAQ 89
Query: 315 VSWRNVFLAEYAGPLFVYLWVYQRP---WILYGTQTSSPEQVATVAAICWSAHYAKRLFE 485
VS+R +F EY GPL +Y Y P ++ YG + V T A W HY KR+ E
Sbjct: 90 VSYRTLFFFEYLGPLLIYPVFYYFPVYKFLGYG-EDCVIHPVQTYAMYYWCFHYFKRILE 148
Query: 486 TLFIHRFS 509
T F+HRFS
Sbjct: 149 TFFVHRFS 156
>At3g57940.1 68416.m06458 expressed protein contains Pfam profile
PF05127: Putative ATPase (DUF699)
Length = 1024
Score = 31.1 bits (67), Expect = 0.75
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = +1
Query: 481 LKLCSFIAFPWYDAIEELVQKLCILLVVCPLCGLSCEPSPIHCSLYYL 624
++L I + D IE + L L V L +C PSP C LYY+
Sbjct: 468 IELTESIRYGSGDPIESWLNGLLCLDVATCLPNPACHPSPSQCDLYYV 515
>At5g60800.1 68418.m07628 heavy-metal-associated domain-containing
protein similar to farnesylated protein ATFP3
[GI:4097547]; contains Pfam profile PF00403:
Heavy-metal-associated domain
Length = 283
Score = 29.1 bits (62), Expect = 3.0
Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 2/56 (3%)
Frame = +1
Query: 91 SVSSAKPLGKIHVTGESSIKNVKEKIQQSVKK--SLYPDRQALKLEAKGKTLKDED 252
+V S GK+ VTG ++EK+++ KK L + + E + K DED
Sbjct: 55 TVKSESATGKLTVTGALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDED 110
>At5g16010.1 68418.m01872 3-oxo-5-alpha-steroid 4-dehydrogenase
family protein / steroid 5-alpha-reductase family
protein similar to steroid 5alpha-reductase - Rattus
norvegicus, PIR:A34239 [SP|24008]; contains Pfam
3-oxo-5-alpha-steroid 4-dehydrogenase domain PF02544
Length = 268
Score = 29.1 bits (62), Expect = 3.0
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = +3
Query: 462 HYAKRLFETLFIHRFS 509
H+ KR+FE LFIH++S
Sbjct: 104 HFFKRVFEVLFIHKYS 119
>At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to
polyubiquitin (ubq8) GI:870793, GB:L05917 [Arabidopsis
thaliana] (Genetics 139 (2), 921-939 (1995))
Length = 631
Score = 29.1 bits (62), Expect = 3.0
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +1
Query: 121 IHVTGESSIKNVKEKIQQSVKKSLYPDRQAL 213
+ V +I NVKEKIQ VK+ + PD+Q L
Sbjct: 566 LEVESSDTIANVKEKIQ--VKEGIKPDQQML 594
>At5g36740.1 68418.m04402 PHD finger family protein
Length = 1179
Score = 28.7 bits (61), Expect = 4.0
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = +1
Query: 127 VTGESSIKNVKEKIQQSVKKSLYPD 201
V+G SS+ VKE+++Q KSL P+
Sbjct: 90 VSGGSSVDEVKEEVKQECVKSLVPE 114
>At5g36670.1 68418.m04388 PHD finger family protein
Length = 1193
Score = 28.7 bits (61), Expect = 4.0
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = +1
Query: 127 VTGESSIKNVKEKIQQSVKKSLYPD 201
V+G SS+ VKE+++Q KSL P+
Sbjct: 90 VSGGSSVDEVKEEVKQECVKSLVPE 114
>At5g06930.1 68418.m00783 expressed protein
Length = 657
Score = 28.3 bits (60), Expect = 5.3
Identities = 15/46 (32%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = +1
Query: 445 QYVGQHTMPKGCLKLC-SFIAFPWYDAIEELVQKLCILLVVCPLCG 579
+ +G + P C +L SF W +A EE +Q+LC + CG
Sbjct: 357 EMIGNFSEPATCDRLQESFSVNLWKEAFEEALQRLCPVQATRRQCG 402
>At3g18080.1 68416.m02299 glycosyl hydrolase family 1 protein
contains Pfam PF00232 : Glycosyl hydrolase family 1
domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase;
similar to beta-glucosidase BGQ60 precursor GB:A57512
[Hordeum vulgare]; similar to beta-mannosidase enzyme
(GI:17226270) [Lycopersicon esculentum]
Length = 512
Score = 28.3 bits (60), Expect = 5.3
Identities = 15/39 (38%), Positives = 18/39 (46%), Gaps = 4/39 (10%)
Frame = +3
Query: 297 KDLGPQVSWRNVF----LAEYAGPLFVYLWVYQRPWILY 401
KDLG Q W F L + GP W+Y PW +Y
Sbjct: 362 KDLGYQQDWNVEFGFAKLGKPIGPRAYSSWLYNVPWGMY 400
>At1g10490.1 68414.m01181 expressed protein contains Pfam profile
PF05127: Putative ATPase (DUF699)
Length = 1028
Score = 28.3 bits (60), Expect = 5.3
Identities = 16/48 (33%), Positives = 22/48 (45%)
Frame = +1
Query: 481 LKLCSFIAFPWYDAIEELVQKLCILLVVCPLCGLSCEPSPIHCSLYYL 624
++L I + D IE + L L V L +C P P C LYY+
Sbjct: 453 IELSESIRYASGDPIESWLNGLLCLDVANCLPNPACHPLPSQCDLYYV 500
>At3g26490.1 68416.m03304 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 588
Score = 27.9 bits (59), Expect = 7.0
Identities = 22/77 (28%), Positives = 35/77 (45%), Gaps = 1/77 (1%)
Frame = +1
Query: 397 YMVHKRQVLSK*LQLQQYV-GQHTMPKGCLKLCSFIAFPWYDAIEELVQKLCILLVVCPL 573
Y++HK +LSK L+L+ V Q T ++ + FP EL K C + + L
Sbjct: 39 YLLHKFPMLSKCLRLKNLVSSQETETSQEQQVIQLVDFPGETEAFELCAKFCYGITI-TL 97
Query: 574 CGLSCEPSPIHCSLYYL 624
C + + C+ YL
Sbjct: 98 CAHNV--VAVRCAAEYL 112
>At3g18200.1 68416.m02315 nodulin MtN21 family protein similar to
MtN21 GI:2598575 (root nodule development) from
[Medicago truncatula]
Length = 360
Score = 27.9 bits (59), Expect = 7.0
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +3
Query: 315 VSWRNVFLAEYAGPLFVYLWVYQRPWILY 401
VSW +F YAG + L VY + W +Y
Sbjct: 244 VSWEELFTILYAGIIASGLVVYLQTWCIY 272
>At2g20142.1 68415.m02355 expressed protein
Length = 159
Score = 27.9 bits (59), Expect = 7.0
Identities = 13/35 (37%), Positives = 18/35 (51%), Gaps = 5/35 (14%)
Frame = +3
Query: 363 VYLWVYQRPWILYGTQTSS-----PEQVATVAAIC 452
V+ W + PW Y TQ S+ PE + V A+C
Sbjct: 82 VWKWTWPIPWFHYQTQRSNRFGSFPEMLGLVCALC 116
>At5g41610.2 68418.m05055 cation/hydrogen exchanger, putative
(CHX18) monovalent cation:proton antiporter family 2
(CPA2) member, PMID:11500563
Length = 742
Score = 27.5 bits (58), Expect = 9.3
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = +1
Query: 109 PLGKIHVTGESSIKNVKEKIQQSVKKSLYPDRQALKLEAKGKTLKDED 252
P+G + ++ ESS K IQQ + PD A + E T KD D
Sbjct: 695 PVGSLLISPESSTKASVLVIQQYNGTGIAPDLGAAETEVLTSTDKDSD 742
>At5g41610.1 68418.m05056 cation/hydrogen exchanger, putative
(CHX18) monovalent cation:proton antiporter family 2
(CPA2) member, PMID:11500563
Length = 810
Score = 27.5 bits (58), Expect = 9.3
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = +1
Query: 109 PLGKIHVTGESSIKNVKEKIQQSVKKSLYPDRQALKLEAKGKTLKDED 252
P+G + ++ ESS K IQQ + PD A + E T KD D
Sbjct: 763 PVGSLLISPESSTKASVLVIQQYNGTGIAPDLGAAETEVLTSTDKDSD 810
>At3g57060.1 68416.m06353 non-SMC condensin subunit, XCAP-D2/Cnd1
family protein similar to 13S condensin XCAP-D2 subunit
[Xenopus laevis] GI:3764087; contains Pfam profile
PF04594: Non-SMC condensin subunit, XCAP-D2/Cnd1
Length = 1439
Score = 27.5 bits (58), Expect = 9.3
Identities = 15/46 (32%), Positives = 22/46 (47%)
Frame = -3
Query: 611 EQCIGDGSHDKPHRGQTTNNMHSF*TSSSMASYHGKAMNEQSFKQP 474
E C GDG D H +TT +H S S +G+ ++E+ P
Sbjct: 484 ESCNGDGEIDDLHL-ETTTKIHQDSLSDSCQPENGEEISEKDVSVP 528
>At2g04430.1 68415.m00447 MutT/nudix family protein similar to
SP|P53370 Nucleoside diphosphate-linked moiety X motif 6
{Homo sapiens}; contains Pfam profile PF00293: NUDIX
domain
Length = 283
Score = 27.5 bits (58), Expect = 9.3
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = +2
Query: 575 VAYHVNHPLYTAPCTTCYYIGLGGFVLCELGNLSI 679
+ + + P T PC + IG+G FVL + G + +
Sbjct: 87 LTFWLPEPPSTLPCNASHRIGIGAFVLNKNGEMLV 121
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,705,794
Number of Sequences: 28952
Number of extensions: 343910
Number of successful extensions: 916
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 881
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 915
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1535986264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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