BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021748 (740 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 145 7e-36 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 136 4e-33 SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 134 9e-33 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 119 5e-28 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 105 5e-24 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 64 2e-11 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 59 8e-10 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 38 0.002 SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 30 0.40 SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 27 3.7 SPBC146.04 |||sulfhydryl oxidase |Schizosaccharomyces pombe|chr ... 26 6.5 SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha... 26 6.5 SPCC1322.09 |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.6 SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr ... 25 8.6 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 145 bits (351), Expect = 7e-36 Identities = 67/90 (74%), Positives = 79/90 (87%) Frame = +3 Query: 252 HGTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTT 431 +GTVIGIDLGTTYSCV V KNGRVEIIANDQGNRITPSYVAFT+D ERL+G+AAKNQ + Sbjct: 34 YGTVIGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTED-ERLVGEAAKNQAPS 92 Query: 432 NPENTVFDAKRLIGREWSDQTVQHDVSSSP 521 NPENT+FD KRLIGR++ ++T+ D+ S P Sbjct: 93 NPENTIFDIKRLIGRKFDEKTMAKDIKSFP 122 Score = 116 bits (278), Expect = 5e-27 Identities = 55/77 (71%), Positives = 62/77 (80%) Frame = +2 Query: 509 KFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVP 688 K FPF +V ++P V+V K F PEEISAM+L+KMK+TAEAYLGK VTHAVVTVP Sbjct: 119 KSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMILSKMKQTAEAYLGKPVTHAVVTVP 178 Query: 689 AYFNDAQRQATKDAGTI 739 AYFNDAQRQATKDAGTI Sbjct: 179 AYFNDAQRQATKDAGTI 195 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 136 bits (328), Expect = 4e-33 Identities = 66/107 (61%), Positives = 79/107 (73%) Frame = +3 Query: 264 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 443 IGIDLGTTYSCVG + N RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ NP N Sbjct: 5 IGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHN 63 Query: 444 TVFDAKRLIGREWSDQTVQHDVSSSPSRSLKRTANLMFKYKLHKATK 584 T+FDAKRLIGR ++D VQ D+ P + +++ + + + TK Sbjct: 64 TIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETK 110 Score = 111 bits (268), Expect = 8e-26 Identities = 54/84 (64%), Positives = 65/84 (77%) Frame = +2 Query: 488 PNCTT*CKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVT 667 P + K +PFKV+EK+ KP +QV+ K F PEEIS+MVL KM+E+AEA+LG KVT Sbjct: 79 PEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSMVLLKMRESAEAFLGGKVT 138 Query: 668 HAVVTVPAYFNDAQRQATKDAGTI 739 AVVTVPAYFND+QRQATKDAG I Sbjct: 139 DAVVTVPAYFNDSQRQATKDAGLI 162 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 134 bits (325), Expect = 9e-33 Identities = 66/107 (61%), Positives = 78/107 (72%) Frame = +3 Query: 264 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 443 IGIDLGTTYSCVG + N RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ NP N Sbjct: 5 IGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHN 63 Query: 444 TVFDAKRLIGREWSDQTVQHDVSSSPSRSLKRTANLMFKYKLHKATK 584 T+FDAKRLIGR++ D VQ D+ P + + + + + + TK Sbjct: 64 TIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETK 110 Score = 112 bits (269), Expect = 6e-26 Identities = 55/84 (65%), Positives = 64/84 (76%) Frame = +2 Query: 488 PNCTT*CKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVT 667 P + K +PFKV+ K+ KP +QV+ K F PEEIS+MVL KM+ETAEAYLG KVT Sbjct: 79 PEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSMVLMKMRETAEAYLGGKVT 138 Query: 668 HAVVTVPAYFNDAQRQATKDAGTI 739 AVVTVPAYFND+QRQATKDAG I Sbjct: 139 DAVVTVPAYFNDSQRQATKDAGLI 162 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 119 bits (286), Expect = 5e-28 Identities = 57/86 (66%), Positives = 64/86 (74%) Frame = +3 Query: 264 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 443 IGIDLGTTYSCV V++ VEII NDQG R TPS+VAFT+ ERL+GDAAKNQ NP N Sbjct: 9 IGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTET-ERLVGDAAKNQAAMNPRN 67 Query: 444 TVFDAKRLIGREWSDQTVQHDVSSSP 521 TVFDAKRLIGR + D Q D+ P Sbjct: 68 TVFDAKRLIGRRYEDPETQKDIKHWP 93 Score = 92.7 bits (220), Expect = 5e-20 Identities = 45/77 (58%), Positives = 56/77 (72%) Frame = +2 Query: 509 KFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVP 688 K +PFKV++ N P ++V K F +EISAMVLTKMKE +EA L K+V AV+TVP Sbjct: 90 KHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAMVLTKMKEISEAKLNKRVEKAVITVP 149 Query: 689 AYFNDAQRQATKDAGTI 739 AYF+D+QR ATKDAG I Sbjct: 150 AYFSDSQRAATKDAGAI 166 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 105 bits (253), Expect = 5e-24 Identities = 49/96 (51%), Positives = 65/96 (67%) Frame = +3 Query: 255 GTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTN 434 G VIGIDLGTT SC+ + + ++IAN +G R TPS VAFT+DGERL+G +AK Q N Sbjct: 50 GPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVIN 109 Query: 435 PENTVFDAKRLIGREWSDQTVQHDVSSSPSRSLKRT 542 PENT F KRLIGR + + VQ D+ P + ++ + Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHS 145 Score = 79.0 bits (186), Expect = 7e-16 Identities = 41/77 (53%), Positives = 56/77 (72%) Frame = +2 Query: 509 KFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVP 688 K P+K+VE +S ++ ++G K ++P +I +L+KM+ETA YLGK V +AVVTVP Sbjct: 135 KEVPYKIVE-HSNGDAWLE-ARG-KTYSPSQIGGFILSKMRETASTYLGKDVKNAVVTVP 191 Query: 689 AYFNDAQRQATKDAGTI 739 AYFND+QRQATK AG I Sbjct: 192 AYFNDSQRQATKAAGAI 208 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 64.5 bits (150), Expect = 2e-11 Identities = 32/86 (37%), Positives = 53/86 (61%) Frame = +3 Query: 261 VIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPE 440 V+GID G + + + V +N +++I N+ NR TPS V++ + R +G+AAK+ +N Sbjct: 7 VVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERS-RFLGEAAKSAEASNFR 65 Query: 441 NTVFDAKRLIGREWSDQTVQHDVSSS 518 NTV KRL GR + D ++ D+ S+ Sbjct: 66 NTVGSLKRLAGRTYDDPEIK-DIESN 90 Score = 44.8 bits (101), Expect = 1e-05 Identities = 25/76 (32%), Positives = 40/76 (52%) Frame = +2 Query: 512 FFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPA 691 F K+ E + +VQ + F+ ++ A TK+K AEA L V+ V+++PA Sbjct: 91 FISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAYFTKIKAIAEAELIGSVSDVVISIPA 150 Query: 692 YFNDAQRQATKDAGTI 739 +F D QR+A +A I Sbjct: 151 WFTDIQRRALLEAANI 166 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 58.8 bits (136), Expect = 8e-10 Identities = 28/82 (34%), Positives = 49/82 (59%) Frame = +3 Query: 258 TVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNP 437 TVIGI G S + ++G+ +++AN++GNR PS +++ D E G A+ QL N Sbjct: 25 TVIGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQE-YHGVQARGQLVRNA 83 Query: 438 ENTVFDAKRLIGREWSDQTVQH 503 +N+V + + L+G+ + T+ H Sbjct: 84 DNSVTNFRDLLGKSHDELTLHH 105 Score = 48.4 bits (110), Expect = 1e-06 Identities = 22/51 (43%), Positives = 32/51 (62%) Frame = +2 Query: 578 DKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDA 730 +K+ E S L ++ E+AE +LG KV V++VP YF DAQR+A + A Sbjct: 135 EKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESA 185 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 37.5 bits (83), Expect = 0.002 Identities = 19/51 (37%), Positives = 28/51 (54%) Frame = +2 Query: 587 FAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTI 739 ++ EEI AM + AE +K+T V+TVP +FN+ QR +A I Sbjct: 135 YSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSILLEAARI 185 >SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizosaccharomyces pombe|chr 2|||Manual Length = 1706 Score = 29.9 bits (64), Expect = 0.40 Identities = 16/42 (38%), Positives = 22/42 (52%) Frame = +3 Query: 228 RQEGER*GHGTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNR 353 R+ GER V G LG TY+ V ++G VE+ D N+ Sbjct: 1266 RRNGERNRFSVVNGCFLGNTYAFASVTQDGSVEVHRLDVNNQ 1307 >SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual Length = 4717 Score = 26.6 bits (56), Expect = 3.7 Identities = 12/41 (29%), Positives = 20/41 (48%) Frame = -2 Query: 730 SIFGCLTLSIIEISRHCNNSMSNLFSKVSLSSLLHFSQNHS 608 +I G + L+ I + N +N+F L+ L HF + S Sbjct: 2586 NILGRIALAFTSIDENANLESANIFEHARLALLQHFMDHSS 2626 >SPBC146.04 |||sulfhydryl oxidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 192 Score = 25.8 bits (54), Expect = 6.5 Identities = 11/49 (22%), Positives = 24/49 (48%) Frame = -2 Query: 274 SIPMTVPCPYLSPSCRRHKRPRPPKLARTNSTAS*PCKIYRLKNSEINK 128 S +T+PC S ++ PP+ + + + CK++ N ++N+ Sbjct: 102 SSAITMPCGEYSVELQKILDVHPPQTSSRKAATTWACKVHNQLNEKMNQ 150 >SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccharomyces pombe|chr 1|||Manual Length = 925 Score = 25.8 bits (54), Expect = 6.5 Identities = 9/14 (64%), Positives = 11/14 (78%) Frame = -2 Query: 379 VKATYEGVMRLPWS 338 +K TYEG +R PWS Sbjct: 284 MKLTYEGELRAPWS 297 >SPCC1322.09 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 455 Score = 25.4 bits (53), Expect = 8.6 Identities = 22/75 (29%), Positives = 35/75 (46%), Gaps = 4/75 (5%) Frame = +3 Query: 336 NDQGNRITPSYVAFTQDGERLIG--DAAKNQLTTNPENTVFDA--KRLIGREWSDQTVQH 503 ND PS V QD + L+ D +N N +N + +A + S++ QH Sbjct: 34 NDDSKIGAPSEV---QDEKELLEFLDQLENDEEKNEKNPIEEANEENESVAATSNERQQH 90 Query: 504 DVSSSPSRSLKRTAN 548 D S+ PS++ + T N Sbjct: 91 DASNQPSQAAQTTIN 105 >SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 622 Score = 25.4 bits (53), Expect = 8.6 Identities = 9/20 (45%), Positives = 13/20 (65%) Frame = +3 Query: 387 GERLIGDAAKNQLTTNPENT 446 GE ++ D+ N TNP+NT Sbjct: 6 GENIVSDSIVNMELTNPDNT 25 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,914,273 Number of Sequences: 5004 Number of extensions: 58839 Number of successful extensions: 182 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 167 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 178 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 351258950 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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