SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021748
         (740 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M...   145   7e-36
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ...   136   4e-33
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p...   134   9e-33
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc...   119   5e-28
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce...   105   5e-24
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch...    64   2e-11
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom...    59   8e-10
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe...    38   0.002
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos...    30   0.40 
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual        27   3.7  
SPBC146.04 |||sulfhydryl oxidase |Schizosaccharomyces pombe|chr ...    26   6.5  
SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha...    26   6.5  
SPCC1322.09 |||conserved fungal protein|Schizosaccharomyces pomb...    25   8.6  
SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr ...    25   8.6  

>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 663

 Score =  145 bits (351), Expect = 7e-36
 Identities = 67/90 (74%), Positives = 79/90 (87%)
 Frame = +3

Query: 252 HGTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTT 431
           +GTVIGIDLGTTYSCV V KNGRVEIIANDQGNRITPSYVAFT+D ERL+G+AAKNQ  +
Sbjct: 34  YGTVIGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTED-ERLVGEAAKNQAPS 92

Query: 432 NPENTVFDAKRLIGREWSDQTVQHDVSSSP 521
           NPENT+FD KRLIGR++ ++T+  D+ S P
Sbjct: 93  NPENTIFDIKRLIGRKFDEKTMAKDIKSFP 122



 Score =  116 bits (278), Expect = 5e-27
 Identities = 55/77 (71%), Positives = 62/77 (80%)
 Frame = +2

Query: 509 KFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVP 688
           K FPF +V   ++P V+V      K F PEEISAM+L+KMK+TAEAYLGK VTHAVVTVP
Sbjct: 119 KSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMILSKMKQTAEAYLGKPVTHAVVTVP 178

Query: 689 AYFNDAQRQATKDAGTI 739
           AYFNDAQRQATKDAGTI
Sbjct: 179 AYFNDAQRQATKDAGTI 195


>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 644

 Score =  136 bits (328), Expect = 4e-33
 Identities = 66/107 (61%), Positives = 79/107 (73%)
 Frame = +3

Query: 264 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 443
           IGIDLGTTYSCVG + N RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+  NP N
Sbjct: 5   IGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHN 63

Query: 444 TVFDAKRLIGREWSDQTVQHDVSSSPSRSLKRTANLMFKYKLHKATK 584
           T+FDAKRLIGR ++D  VQ D+   P + +++    + + +    TK
Sbjct: 64  TIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETK 110



 Score =  111 bits (268), Expect = 8e-26
 Identities = 54/84 (64%), Positives = 65/84 (77%)
 Frame = +2

Query: 488 PNCTT*CKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVT 667
           P   +  K +PFKV+EK+ KP +QV+     K F PEEIS+MVL KM+E+AEA+LG KVT
Sbjct: 79  PEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSMVLLKMRESAEAFLGGKVT 138

Query: 668 HAVVTVPAYFNDAQRQATKDAGTI 739
            AVVTVPAYFND+QRQATKDAG I
Sbjct: 139 DAVVTVPAYFNDSQRQATKDAGLI 162


>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 647

 Score =  134 bits (325), Expect = 9e-33
 Identities = 66/107 (61%), Positives = 78/107 (72%)
 Frame = +3

Query: 264 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 443
           IGIDLGTTYSCVG + N RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+  NP N
Sbjct: 5   IGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHN 63

Query: 444 TVFDAKRLIGREWSDQTVQHDVSSSPSRSLKRTANLMFKYKLHKATK 584
           T+FDAKRLIGR++ D  VQ D+   P + + +    + + +    TK
Sbjct: 64  TIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETK 110



 Score =  112 bits (269), Expect = 6e-26
 Identities = 55/84 (65%), Positives = 64/84 (76%)
 Frame = +2

Query: 488 PNCTT*CKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVT 667
           P   +  K +PFKV+ K+ KP +QV+     K F PEEIS+MVL KM+ETAEAYLG KVT
Sbjct: 79  PEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSMVLMKMRETAEAYLGGKVT 138

Query: 668 HAVVTVPAYFNDAQRQATKDAGTI 739
            AVVTVPAYFND+QRQATKDAG I
Sbjct: 139 DAVVTVPAYFNDSQRQATKDAGLI 162


>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 613

 Score =  119 bits (286), Expect = 5e-28
 Identities = 57/86 (66%), Positives = 64/86 (74%)
 Frame = +3

Query: 264 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 443
           IGIDLGTTYSCV V++   VEII NDQG R TPS+VAFT+  ERL+GDAAKNQ   NP N
Sbjct: 9   IGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTET-ERLVGDAAKNQAAMNPRN 67

Query: 444 TVFDAKRLIGREWSDQTVQHDVSSSP 521
           TVFDAKRLIGR + D   Q D+   P
Sbjct: 68  TVFDAKRLIGRRYEDPETQKDIKHWP 93



 Score = 92.7 bits (220), Expect = 5e-20
 Identities = 45/77 (58%), Positives = 56/77 (72%)
 Frame = +2

Query: 509 KFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVP 688
           K +PFKV++ N  P ++V      K F  +EISAMVLTKMKE +EA L K+V  AV+TVP
Sbjct: 90  KHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAMVLTKMKEISEAKLNKRVEKAVITVP 149

Query: 689 AYFNDAQRQATKDAGTI 739
           AYF+D+QR ATKDAG I
Sbjct: 150 AYFSDSQRAATKDAGAI 166


>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 674

 Score =  105 bits (253), Expect = 5e-24
 Identities = 49/96 (51%), Positives = 65/96 (67%)
 Frame = +3

Query: 255 GTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTN 434
           G VIGIDLGTT SC+ + +    ++IAN +G R TPS VAFT+DGERL+G +AK Q   N
Sbjct: 50  GPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVIN 109

Query: 435 PENTVFDAKRLIGREWSDQTVQHDVSSSPSRSLKRT 542
           PENT F  KRLIGR + +  VQ D+   P + ++ +
Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHS 145



 Score = 79.0 bits (186), Expect = 7e-16
 Identities = 41/77 (53%), Positives = 56/77 (72%)
 Frame = +2

Query: 509 KFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVP 688
           K  P+K+VE +S     ++ ++G K ++P +I   +L+KM+ETA  YLGK V +AVVTVP
Sbjct: 135 KEVPYKIVE-HSNGDAWLE-ARG-KTYSPSQIGGFILSKMRETASTYLGKDVKNAVVTVP 191

Query: 689 AYFNDAQRQATKDAGTI 739
           AYFND+QRQATK AG I
Sbjct: 192 AYFNDSQRQATKAAGAI 208


>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
           Pss1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 720

 Score = 64.5 bits (150), Expect = 2e-11
 Identities = 32/86 (37%), Positives = 53/86 (61%)
 Frame = +3

Query: 261 VIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPE 440
           V+GID G + + + V +N  +++I N+  NR TPS V++ +   R +G+AAK+   +N  
Sbjct: 7   VVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERS-RFLGEAAKSAEASNFR 65

Query: 441 NTVFDAKRLIGREWSDQTVQHDVSSS 518
           NTV   KRL GR + D  ++ D+ S+
Sbjct: 66  NTVGSLKRLAGRTYDDPEIK-DIESN 90



 Score = 44.8 bits (101), Expect = 1e-05
 Identities = 25/76 (32%), Positives = 40/76 (52%)
 Frame = +2

Query: 512 FFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPA 691
           F   K+ E +     +VQ    +  F+  ++ A   TK+K  AEA L   V+  V+++PA
Sbjct: 91  FISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAYFTKIKAIAEAELIGSVSDVVISIPA 150

Query: 692 YFNDAQRQATKDAGTI 739
           +F D QR+A  +A  I
Sbjct: 151 WFTDIQRRALLEAANI 166


>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 566

 Score = 58.8 bits (136), Expect = 8e-10
 Identities = 28/82 (34%), Positives = 49/82 (59%)
 Frame = +3

Query: 258 TVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNP 437
           TVIGI  G   S +   ++G+ +++AN++GNR  PS +++  D E   G  A+ QL  N 
Sbjct: 25  TVIGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQE-YHGVQARGQLVRNA 83

Query: 438 ENTVFDAKRLIGREWSDQTVQH 503
           +N+V + + L+G+   + T+ H
Sbjct: 84  DNSVTNFRDLLGKSHDELTLHH 105



 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 22/51 (43%), Positives = 32/51 (62%)
 Frame = +2

Query: 578 DKVFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDA 730
           +K+    E S   L ++ E+AE +LG KV   V++VP YF DAQR+A + A
Sbjct: 135 EKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESA 185


>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 848

 Score = 37.5 bits (83), Expect = 0.002
 Identities = 19/51 (37%), Positives = 28/51 (54%)
 Frame = +2

Query: 587 FAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTI 739
           ++ EEI AM +      AE    +K+T  V+TVP +FN+ QR    +A  I
Sbjct: 135 YSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSILLEAARI 185


>SPBC119.07 |ppk19||serine/threonine protein kinase
            Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1706

 Score = 29.9 bits (64), Expect = 0.40
 Identities = 16/42 (38%), Positives = 22/42 (52%)
 Frame = +3

Query: 228  RQEGER*GHGTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNR 353
            R+ GER     V G  LG TY+   V ++G VE+   D  N+
Sbjct: 1266 RRNGERNRFSVVNGCFLGNTYAFASVTQDGSVEVHRLDVNNQ 1307


>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 4717

 Score = 26.6 bits (56), Expect = 3.7
 Identities = 12/41 (29%), Positives = 20/41 (48%)
 Frame = -2

Query: 730  SIFGCLTLSIIEISRHCNNSMSNLFSKVSLSSLLHFSQNHS 608
            +I G + L+   I  + N   +N+F    L+ L HF  + S
Sbjct: 2586 NILGRIALAFTSIDENANLESANIFEHARLALLQHFMDHSS 2626


>SPBC146.04 |||sulfhydryl oxidase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 192

 Score = 25.8 bits (54), Expect = 6.5
 Identities = 11/49 (22%), Positives = 24/49 (48%)
 Frame = -2

Query: 274 SIPMTVPCPYLSPSCRRHKRPRPPKLARTNSTAS*PCKIYRLKNSEINK 128
           S  +T+PC   S   ++     PP+ +   +  +  CK++   N ++N+
Sbjct: 102 SSAITMPCGEYSVELQKILDVHPPQTSSRKAATTWACKVHNQLNEKMNQ 150


>SPAC16C9.06c |upf1||ATP-dependent RNA helicase
           Upf1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 925

 Score = 25.8 bits (54), Expect = 6.5
 Identities = 9/14 (64%), Positives = 11/14 (78%)
 Frame = -2

Query: 379 VKATYEGVMRLPWS 338
           +K TYEG +R PWS
Sbjct: 284 MKLTYEGELRAPWS 297


>SPCC1322.09 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 455

 Score = 25.4 bits (53), Expect = 8.6
 Identities = 22/75 (29%), Positives = 35/75 (46%), Gaps = 4/75 (5%)
 Frame = +3

Query: 336 NDQGNRITPSYVAFTQDGERLIG--DAAKNQLTTNPENTVFDA--KRLIGREWSDQTVQH 503
           ND      PS V   QD + L+   D  +N    N +N + +A  +       S++  QH
Sbjct: 34  NDDSKIGAPSEV---QDEKELLEFLDQLENDEEKNEKNPIEEANEENESVAATSNERQQH 90

Query: 504 DVSSSPSRSLKRTAN 548
           D S+ PS++ + T N
Sbjct: 91  DASNQPSQAAQTTIN 105


>SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 622

 Score = 25.4 bits (53), Expect = 8.6
 Identities = 9/20 (45%), Positives = 13/20 (65%)
 Frame = +3

Query: 387 GERLIGDAAKNQLTTNPENT 446
           GE ++ D+  N   TNP+NT
Sbjct: 6   GENIVSDSIVNMELTNPDNT 25


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,914,273
Number of Sequences: 5004
Number of extensions: 58839
Number of successful extensions: 182
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 178
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 351258950
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -