Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021747X
(481 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 140 1e-34
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 139 2e-34
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 139 2e-34
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 79 3e-16
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 75 6e-15
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 62 6e-11
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 44 2e-05
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 38 6e-04
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 37 0.001
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.004
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.004
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 29 0.36
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 29 0.36
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 27 1.9
SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|... 26 3.4
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 26 3.4
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 4.5
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 140 bits (338), Expect = 1e-34
Identities = 65/76 (85%), Positives = 68/76 (89%)
Frame = +3
Query: 252 GKGSFKDAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHREAIKNMIRGTSQA 431
GKGSFK AWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGHR+ IKNMI GTSQA
Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQA 109
Query: 432 DCAVLSVAAGTGEFEA 479
DCAVL + GTGEFEA
Sbjct: 110 DCAVLIIGGGTGEFEA 125
Score = 105 bits (252), Expect = 3e-24
Identities = 48/55 (87%), Positives = 50/55 (90%)
Frame = +1
Query: 106 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKDPSK 270
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GK K
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFK 55
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 139 bits (337), Expect = 2e-34
Identities = 64/76 (84%), Positives = 68/76 (89%)
Frame = +3
Query: 252 GKGSFKDAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHREAIKNMIRGTSQA 431
GKGSFK AWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGHR+ IKNMI GTSQA
Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQA 109
Query: 432 DCAVLSVAAGTGEFEA 479
DCA+L + GTGEFEA
Sbjct: 110 DCAILIIGGGTGEFEA 125
Score = 105 bits (252), Expect = 3e-24
Identities = 48/55 (87%), Positives = 50/55 (90%)
Frame = +1
Query: 106 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKDPSK 270
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GK K
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFK 55
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 139 bits (337), Expect = 2e-34
Identities = 64/76 (84%), Positives = 68/76 (89%)
Frame = +3
Query: 252 GKGSFKDAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHREAIKNMIRGTSQA 431
GKGSFK AWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGHR+ IKNMI GTSQA
Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQA 109
Query: 432 DCAVLSVAAGTGEFEA 479
DCA+L + GTGEFEA
Sbjct: 110 DCAILIIGGGTGEFEA 125
Score = 105 bits (252), Expect = 3e-24
Identities = 48/55 (87%), Positives = 50/55 (90%)
Frame = +1
Query: 106 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKDPSK 270
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GK K
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFK 55
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 79.4 bits (187), Expect = 3e-16
Identities = 39/76 (51%), Positives = 48/76 (63%)
Frame = +3
Query: 249 NGKGSFKDAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHREAIKNMIRGTSQ 428
+GKGSF AW+LD + ER RG+T+D+A FE+ K I DAPGHR+ I MI G S
Sbjct: 219 SGKGSFSYAWLLDTTEEERARGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASS 278
Query: 429 ADCAVLSVAAGTGEFE 476
AD AVL V + FE
Sbjct: 279 ADFAVLVVDSSQNNFE 294
Score = 49.6 bits (113), Expect = 2e-07
Identities = 21/47 (44%), Positives = 32/47 (68%)
Frame = +1
Query: 118 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 258
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GK
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGK 221
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 74.9 bits (176), Expect = 6e-15
Identities = 35/76 (46%), Positives = 48/76 (63%)
Frame = +3
Query: 252 GKGSFKDAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHREAIKNMIRGTSQA 431
GK S+ +W LD ERE+G T+++ FET +++DAPGH+ + NMI G SQA
Sbjct: 281 GKESWYLSWALDSTSEEREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQA 340
Query: 432 DCAVLSVAAGTGEFEA 479
D VL ++A GEFEA
Sbjct: 341 DIGVLVISARRGEFEA 356
Score = 67.3 bits (157), Expect = 1e-12
Identities = 28/48 (58%), Positives = 39/48 (81%)
Frame = +1
Query: 118 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKD 261
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK+
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKE 283
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 61.7 bits (143), Expect = 6e-11
Identities = 30/73 (41%), Positives = 44/73 (60%)
Frame = +3
Query: 252 GKGSFKDAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHREAIKNMIRGTSQA 431
G+ SF D +DK E+ RGITI A ++ET+ + +D PGH + IKNMI G +
Sbjct: 81 GQASFMDYSQIDKAPEEKARGITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATM 140
Query: 432 DCAVLSVAAGTGE 470
D A++ V+A G+
Sbjct: 141 DGAIIVVSATDGQ 153
Score = 35.1 bits (77), Expect = 0.006
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +1
Query: 112 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 198
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 43.6 bits (98), Expect = 2e-05
Identities = 23/63 (36%), Positives = 32/63 (50%)
Frame = +3
Query: 279 VLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHREAIKNMIRGTSQADCAVLSVAA 458
V+D L AER+RGITI+ A F + +ID PGH + + R + D AV +
Sbjct: 67 VMDYLPAERQRGITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDG 126
Query: 459 GTG 467
G
Sbjct: 127 SAG 129
Score = 28.7 bits (61), Expect = 0.48
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +1
Query: 130 NIVVIGHVDSGKSTTTGHLIYKCG 201
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 38.3 bits (85), Expect = 6e-04
Identities = 20/66 (30%), Positives = 33/66 (50%), Gaps = 4/66 (6%)
Frame = +3
Query: 282 LDKLKAERERGITIDI----ALWKFETSKYYVTIIDAPGHREAIKNMIRGTSQADCAVLS 449
LDKL+ ER RGIT+ ++ + Y + +ID PGH + ++ + + +L
Sbjct: 95 LDKLEVERRRGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILL 154
Query: 450 VAAGTG 467
V A G
Sbjct: 155 VDASQG 160
Score = 24.6 bits (51), Expect = 7.8
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1
Query: 130 NIVVIGHVDSGKSTTTGHLIYKCGGIDK 213
N VI H+D GKST + ++ G I++
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINE 87
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 37.1 bits (82), Expect = 0.001
Identities = 24/79 (30%), Positives = 35/79 (44%), Gaps = 2/79 (2%)
Frame = +3
Query: 237 GGPGNGKGSFKDAWVLDKLKAERERGITIDIALWK--FETSKYYVTIIDAPGHREAIKNM 410
G +GK + DA+ + + GIT I + F+ ++T +D PGH
Sbjct: 178 GHVDHGKTTLLDAFRKSTIASTEHGGITQKIGAFTVPFDKGSKFITFLDTPGHMAFEAMR 237
Query: 411 IRGTSQADCAVLSVAAGTG 467
RG + AD VL VA G
Sbjct: 238 KRGANIADIVVLVVAGDDG 256
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.004
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 106 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 201
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.004
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +1
Query: 106 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 201
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 29.1 bits (62), Expect = 0.36
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +3
Query: 360 YVTIIDAPGHREAIKNMIRGTSQADCAVLSVA 455
+V+ +D PGH + M+ G + D A+L +A
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIA 140
Score = 25.0 bits (52), Expect = 5.9
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +1
Query: 100 PKMGKEKTHINIVVIGHVDSGKST 171
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 29.1 bits (62), Expect = 0.36
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +1
Query: 130 NIVVIGHVDSGKSTTTGHLIYKCGGI 207
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
Score = 27.9 bits (59), Expect = 0.84
Identities = 27/81 (33%), Positives = 37/81 (45%), Gaps = 19/81 (23%)
Frame = +3
Query: 282 LDKLKAERERGITIDIA----LWK---------------FETSKYYVTIIDAPGHREAIK 404
+D ++ ERE+GITI A W+ FE S Y + IID PGH +
Sbjct: 102 MDFMELEREKGITIQSAATHCTWERTVDQIEANEKQKTDFEKS-YNINIIDTPGHIDFTI 160
Query: 405 NMIRGTSQADCAVLSVAAGTG 467
+ R D AVL + A +G
Sbjct: 161 EVERALRVLDGAVLVLCAVSG 181
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 26.6 bits (56), Expect = 1.9
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +3
Query: 369 IIDAPGHREAIKNMIRGTSQADCAVLSV 452
IID PGH RGTS + A+L +
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1234
Score = 25.8 bits (54), Expect = 3.4
Identities = 23/78 (29%), Positives = 38/78 (48%), Gaps = 3/78 (3%)
Frame = -3
Query: 452 YAEHSAISLRGSSDHVLDGLSVSRSINDGNIVLASFELPE---SNIDCDTTLTLSL*FVQ 282
++ S+ R S+ +LD L S S ++ + EL E S++ C + + F
Sbjct: 17 HSPESSRETRFSAQQLLDELKDSYS--SPSVAIQLLELNEQAFSSLGCKLDIHIVQHFSL 74
Query: 281 YPSIFEGSFPISWASFSN 228
S+FE S ++W SFSN
Sbjct: 75 --SLFETSVGMNWKSFSN 90
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 25.8 bits (54), Expect = 3.4
Identities = 14/53 (26%), Positives = 21/53 (39%), Gaps = 2/53 (3%)
Frame = +3
Query: 219 HREVREGGPGNGKGSFKDAWVLDKLKAERERGITIDIA--LWKFETSKYYVTI 371
H G G ++WVLD L E+ I+ +A +WK V +
Sbjct: 51 HENANAAGKEYGDSGVSESWVLDFLSVTGEKTISEFLAQKIWKTSNGDLNVAV 103
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.4 bits (53), Expect = 4.5
Identities = 9/26 (34%), Positives = 14/26 (53%)
Frame = -1
Query: 268 LKDPFPFPGPPSRTSRWYVCQYHHIC 191
+ P P PP +T + Y QY+ +C
Sbjct: 1 MSTPLIPPAPPKKTLQLYTPQYYGLC 26
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,912,932
Number of Sequences: 5004
Number of extensions: 35460
Number of successful extensions: 150
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 184020746
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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