Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021746
(736 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g22320.1 68418.m02604 leucine-rich repeat family protein cont... 31 0.60
At1g08550.1 68414.m00948 violaxanthin de-epoxidase precursor, pu... 31 0.79
At5g40520.1 68418.m04916 expressed protein 30 1.4
At1g77840.1 68414.m09070 eukaryotic translation initiation facto... 30 1.4
At3g28770.1 68416.m03591 expressed protein 30 1.8
At5g10010.1 68418.m01159 expressed protein 29 2.4
At2g34357.1 68415.m04206 expressed protein 29 3.2
At1g16210.1 68414.m01941 expressed protein ESTs gb|T04357 and gb... 29 3.2
At1g02660.1 68414.m00216 lipase class 3 family protein contains ... 29 3.2
At2g19950.1 68415.m02332 expressed protein contains 2 transmembr... 29 4.2
At1g08340.1 68414.m00922 rac GTPase activating protein, putative... 29 4.2
At5g60370.1 68418.m07570 expressed protein predicted protein, Ar... 28 5.6
At4g02940.1 68417.m00401 oxidoreductase, 2OG-Fe(II) oxygenase fa... 28 5.6
At3g03060.1 68416.m00302 AAA-type ATPase family protein contains... 28 7.4
At1g76780.1 68414.m08935 expressed protein ; expression supporte... 28 7.4
At1g32810.1 68414.m04044 expressed protein 28 7.4
At5g04890.1 68418.m00513 small heat shock-like protein (RTM2) si... 27 9.8
At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains... 27 9.8
>At5g22320.1 68418.m02604 leucine-rich repeat family protein
contains leucine rich repeat (LRR) domains, Pfam:PF00560
Length = 452
Score = 31.5 bits (68), Expect = 0.60
Identities = 16/36 (44%), Positives = 21/36 (58%)
Frame = +2
Query: 266 EKRKAIKQGKRNEAKEIETNDEDIKKPVVKGFSDDN 373
E RK K KRN+++E E N+ED K K S+ N
Sbjct: 295 EDRKRKKSSKRNKSEEEEVNNEDHKSKKKKSKSNTN 330
>At1g08550.1 68414.m00948 violaxanthin de-epoxidase precursor,
putative (AVDE1) similar to EST gb|N37612
Length = 462
Score = 31.1 bits (67), Expect = 0.79
Identities = 14/40 (35%), Positives = 26/40 (65%), Gaps = 1/40 (2%)
Frame = +2
Query: 239 TCQKKAEIVEK-RKAIKQGKRNEAKEIETNDEDIKKPVVK 355
TC + +VE+ K +++G+R KE+E +E+++K V K
Sbjct: 361 TCGPEPALVERIEKTVEEGERIIVKEVEEIEEEVEKEVEK 400
>At5g40520.1 68418.m04916 expressed protein
Length = 693
Score = 30.3 bits (65), Expect = 1.4
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +2
Query: 203 KTDSQAKAKGRKTCQKKAEIVEKRKAIKQGKRNEAKEIETNDEDI 337
+TD ++ G Q+K E+ EK++A Q K +EA + E I
Sbjct: 631 ETDERSYLGGESRLQEKVEVSEKKQASSQAKSDEAIILSVVKEQI 675
>At1g77840.1 68414.m09070 eukaryotic translation initiation factor
5, putative / eIF-5, putative similar to SP|P55876
Eukaryotic translation initiation factor 5 (eIF-5) {Zea
mays}; contains Pfam profiles PF02020:
eIF4-gamma/eIF5/eIF2-epsilon, PF01873: Domain found in
IF2B/IF5
Length = 437
Score = 30.3 bits (65), Expect = 1.4
Identities = 13/56 (23%), Positives = 31/56 (55%)
Frame = +2
Query: 170 KGAIRD*RSGEKTDSQAKAKGRKTCQKKAEIVEKRKAIKQGKRNEAKEIETNDEDI 337
K +D ++ K + + +G +++ ++ K+KA+ GK ++ + ++DEDI
Sbjct: 150 KKVSKDKKAMRKAEKERLKEGELADEEQRKLKAKKKALSNGKDSKTSKNHSSDEDI 205
>At3g28770.1 68416.m03591 expressed protein
Length = 2081
Score = 29.9 bits (64), Expect = 1.8
Identities = 16/48 (33%), Positives = 29/48 (60%)
Frame = +2
Query: 200 EKTDSQAKAKGRKTCQKKAEIVEKRKAIKQGKRNEAKEIETNDEDIKK 343
E + SQ +K +KK+ +++K K+ K +E K+++ N+ED KK
Sbjct: 1169 ESSKSQKNEVDKK--EKKSSKDQQKKKEKEMKESEEKKLKKNEEDRKK 1214
>At5g10010.1 68418.m01159 expressed protein
Length = 434
Score = 29.5 bits (63), Expect = 2.4
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = +2
Query: 236 KTCQKKAEIVEKRKAIKQGKRNEAKEIETNDEDIKKPVVK 355
K + K E+ +K A + GKR A + +T +D KKPV K
Sbjct: 86 KAEEVKEEVEKKPVARRGGKRKRATKKDTEIKDEKKPVPK 125
>At2g34357.1 68415.m04206 expressed protein
Length = 1280
Score = 29.1 bits (62), Expect = 3.2
Identities = 16/52 (30%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
Frame = +2
Query: 227 KGRKTCQKKAEI-VEKRKAIKQGKRNEAKEIETNDEDIKKPVVKGFSDDNKE 379
K + ++AE VE R I++G+R++ KE+ D D K FS ++ +
Sbjct: 1125 KRKADSDEEAEFDVEGRLVIREGERSKRKELSDADSDAKSSKGSRFSGNSSK 1176
>At1g16210.1 68414.m01941 expressed protein ESTs gb|T04357 and
gb|AA595092 come from this gene
Length = 234
Score = 29.1 bits (62), Expect = 3.2
Identities = 18/52 (34%), Positives = 26/52 (50%)
Frame = +2
Query: 191 RSGEKTDSQAKAKGRKTCQKKAEIVEKRKAIKQGKRNEAKEIETNDEDIKKP 346
R E S+A K + +KKAE K+ K+ E KE+E + +KKP
Sbjct: 39 REAEGPKSKAVKKREEEAEKKAETAAKKLEAKRLAEQEEKELE---KALKKP 87
>At1g02660.1 68414.m00216 lipase class 3 family protein contains
Pfam profile PF01764: Lipase
Length = 713
Score = 29.1 bits (62), Expect = 3.2
Identities = 12/44 (27%), Positives = 27/44 (61%)
Frame = +2
Query: 194 SGEKTDSQAKAKGRKTCQKKAEIVEKRKAIKQGKRNEAKEIETN 325
S EKT+S KA+ + + IVE + +++ ++N++++I +
Sbjct: 225 SAEKTESALKAENGEVSGETKPIVEAEEEVEEEEKNKSRKISAS 268
>At2g19950.1 68415.m02332 expressed protein contains 2 transmembrane
domains; weak similarity to HPSR2 - heavy chain
potential motor protein (GI:871048) [Giardia
intestinalis]
Length = 702
Score = 28.7 bits (61), Expect = 4.2
Identities = 16/45 (35%), Positives = 23/45 (51%)
Frame = +2
Query: 170 KGAIRD*RSGEKTDSQAKAKGRKTCQKKAEIVEKRKAIKQGKRNE 304
K + D S E+ D Q A GRK Q K +K+K +K+ N+
Sbjct: 20 KSVVED-LSEEQNDLQLPASGRKGSQGKRTSSKKKKLVKEESSNK 63
>At1g08340.1 68414.m00922 rac GTPase activating protein, putative
similar to rac GTPase activating protein 1 GI:3695059
from [Lotus japonicus]; contains Pfam profile PF00620:
RhoGAP domain
Length = 331
Score = 28.7 bits (61), Expect = 4.2
Identities = 17/48 (35%), Positives = 26/48 (54%), Gaps = 3/48 (6%)
Frame = +2
Query: 200 EKTDSQAKAKGR---KTCQKKAEIVEKRKAIKQGKRNEAKEIETNDED 334
EKT + +A + C K+AE EK K ++ + +E +E E DED
Sbjct: 222 EKTVREREASSSVVDRRCSKEAEDGEKEKDNEEEEEDEEEEEEEEDED 269
>At5g60370.1 68418.m07570 expressed protein predicted protein,
Arabidopsis thaliana
Length = 413
Score = 28.3 bits (60), Expect = 5.6
Identities = 17/52 (32%), Positives = 30/52 (57%), Gaps = 3/52 (5%)
Frame = +2
Query: 230 GRKTCQKKAEIV---EKRKAIKQGKRNEAKEIETNDEDIKKPVVKGFSDDNK 376
G + C+K+ E V +RK K K +A+ ++ +E +KK VK S+++K
Sbjct: 125 GTEWCEKQMENVLCFGRRKVNKAMKVGQARHLQLEEEVVKKVRVKVESNEDK 176
>At4g02940.1 68417.m00401 oxidoreductase, 2OG-Fe(II) oxygenase
family protein similar to A. thaliana hypothetical
protein T13L16.2, GenBank accession number 2708738;
contains Pfam domain PF03171 2OG-Fe(II) oxygenase
superfamily
Length = 569
Score = 28.3 bits (60), Expect = 5.6
Identities = 11/42 (26%), Positives = 25/42 (59%)
Frame = +2
Query: 233 RKTCQKKAEIVEKRKAIKQGKRNEAKEIETNDEDIKKPVVKG 358
+K KKAE ++++K ++ + + + + T +E++KK G
Sbjct: 118 QKVAAKKAEDLKQKKTEEEAEEDLKEVVATEEEEVKKECFNG 159
>At3g03060.1 68416.m00302 AAA-type ATPase family protein contains a
ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
Length = 639
Score = 27.9 bits (59), Expect = 7.4
Identities = 19/72 (26%), Positives = 39/72 (54%), Gaps = 2/72 (2%)
Frame = +2
Query: 176 AIRD*RSGEKTDSQAKAKGRKTCQKKAEIVEKRKAIKQGKRNEAKEIETN-DEDI-KKPV 349
AIR ++ T+ Q +A+ R+T ++KAEI + +K E + E ED+ ++ +
Sbjct: 200 AIRQEQARRATEEQIQAQRRQTEREKAEIERETIRVKAIAEAEGRAHEARLAEDVNRRML 259
Query: 350 VKGFSDDNKEWL 385
V + + ++W+
Sbjct: 260 VDRANAEREKWV 271
>At1g76780.1 68414.m08935 expressed protein ; expression supported
by MPSS
Length = 1871
Score = 27.9 bits (59), Expect = 7.4
Identities = 15/62 (24%), Positives = 33/62 (53%)
Frame = +2
Query: 194 SGEKTDSQAKAKGRKTCQKKAEIVEKRKAIKQGKRNEAKEIETNDEDIKKPVVKGFSDDN 373
+G+K + K ++ +++ E+ E ++ K+ + E + +TNDE+ K+ + G
Sbjct: 528 TGKKINEDGTRKVQEMIRQQ-ELDEPARSEKENRSRELVKSKTNDEEKKEKEIAGTERKE 586
Query: 374 KE 379
KE
Sbjct: 587 KE 588
>At1g32810.1 68414.m04044 expressed protein
Length = 654
Score = 27.9 bits (59), Expect = 7.4
Identities = 14/38 (36%), Positives = 24/38 (63%), Gaps = 2/38 (5%)
Frame = +2
Query: 227 KGRKTCQKKAEIVEKRKAIK--QGKRNEAKEIETNDED 334
KG++ +K+ + +RK IK + KRNE + E ++ED
Sbjct: 574 KGKRKARKRRRLSIQRKGIKVLRKKRNEEEVSEEDEED 611
>At5g04890.1 68418.m00513 small heat shock-like protein (RTM2)
similar to 17.9 kDa heat-shock protein [Helianthus
annuus] GI:11990130; contains Pfam profile PF00011:
Hsp20/alpha crystallin family; supporting cDNA
gi|7407072|gb|AF208051.1|AF208051; identical to cDNA
small heat shock-like protein (RTM2) GI:7407072, small
heat shock-like protein [Arabidopsis thaliana]
GI:7407073
Length = 366
Score = 27.5 bits (58), Expect = 9.8
Identities = 14/46 (30%), Positives = 24/46 (52%)
Frame = +2
Query: 203 KTDSQAKAKGRKTCQKKAEIVEKRKAIKQGKRNEAKEIETNDEDIK 340
K +AKAK +K E +E ++ +++ K E + E ED+K
Sbjct: 180 KLQEEAKAKEEAAAKKLQEEIEAKEKLEERKLEERRLEERKLEDMK 225
>At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains
ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
Length = 1340
Score = 27.5 bits (58), Expect = 9.8
Identities = 13/60 (21%), Positives = 30/60 (50%)
Frame = +2
Query: 200 EKTDSQAKAKGRKTCQKKAEIVEKRKAIKQGKRNEAKEIETNDEDIKKPVVKGFSDDNKE 379
E+ + + + R +++ I+E+R+ +QG+R ++ + + P + S D KE
Sbjct: 383 ERERQRERERQRARDRERERILERREKERQGERERERKRALEIKRDRTPTARATSKDTKE 442
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,886,892
Number of Sequences: 28952
Number of extensions: 109506
Number of successful extensions: 467
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 444
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 466
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1614253080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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