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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021735
         (716 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g67270.1 68418.m08480 microtubule-associated EB1 family prote...    96   2e-20
At5g62500.1 68418.m07844 microtubule-associated EB1 family prote...    91   5e-19
At3g47690.1 68416.m05194 microtubule-associated EB1 family prote...    91   9e-19
At2g03200.1 68415.m00273 aspartyl protease family protein contai...    30   1.3  
At5g45400.1 68418.m05579 replication protein, putative similar t...    28   5.4  
At3g19090.1 68416.m02426 RNA-binding protein, putative similar t...    28   7.1  

>At5g67270.1 68418.m08480 microtubule-associated EB1 family protein
           similar to SP|Q9UPY8 Microtubule-associated protein
           RP/EB family member 3 (Protein EB3) {Homo sapiens};
           contains Pfam profiles PF00307: Calponin homology (CH)
           domain, PF03271: EB1 protein
          Length = 329

 Score = 96.3 bits (229), Expect = 2e-20
 Identities = 43/95 (45%), Positives = 62/95 (65%), Gaps = 1/95 (1%)
 Frame = +3

Query: 258 YCQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDKIVPIDKLVKGRFQD 437
           +CQ MD + PG+VPM ++ F    E+E IQN+K+LQ  F K+ + K + + KLVKGR  D
Sbjct: 43  HCQLMDSVHPGTVPMHKVNFDAKSEYEMIQNYKVLQDVFNKLKITKHIEVSKLVKGRPLD 102

Query: 438 NFEFLQWFKKFFDANYGGA-AYDAVRRRRSAHGAR 539
           N EF+QW KK+ D+  GG   Y A+ RR ++ G +
Sbjct: 103 NLEFMQWMKKYCDSVNGGQHNYHALERREASKGGK 137



 Score = 38.7 bits (86), Expect = 0.004
 Identities = 14/28 (50%), Positives = 21/28 (75%)
 Frame = +1

Query: 172 LSRHDMLAWVNDCLQSNFAKIEELCTGA 255
           + R ++LAW+N  LQ N +K+EE C+GA
Sbjct: 14  VGRSEILAWINSTLQLNLSKVEEACSGA 41


>At5g62500.1 68418.m07844 microtubule-associated EB1 family protein
           similar to EBF3-S (Microtubule-associated protein) [Homo
           sapiens] GI:12751131; contains Pfam profiles PF00307:
           Calponin homology (CH) domain, PF03271: EB1 protein
          Length = 293

 Score = 91.5 bits (217), Expect = 5e-19
 Identities = 42/89 (47%), Positives = 59/89 (66%), Gaps = 3/89 (3%)
 Frame = +3

Query: 261 CQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDKIVPIDKLVKGRFQDN 440
           CQ +DM FPG VPM ++ F+   E+E IQN+K++Q  F K+ + K + +++LVKGR  DN
Sbjct: 44  CQMLDMTFPGVVPMHKVNFEAKNEYEMIQNYKVMQEVFTKLKITKPLEVNRLVKGRPLDN 103

Query: 441 FEFLQWFKKFFDANYGGAA---YDAVRRR 518
            EFLQW K+F D+  GG     Y+ V RR
Sbjct: 104 LEFLQWLKRFCDSINGGIMNENYNPVERR 132



 Score = 33.5 bits (73), Expect = 0.14
 Identities = 12/28 (42%), Positives = 21/28 (75%)
 Frame = +1

Query: 172 LSRHDMLAWVNDCLQSNFAKIEELCTGA 255
           + R+++L+W+ND L  N ++IEE  +GA
Sbjct: 14  VGRNEILSWINDRLHLNLSRIEEAASGA 41


>At3g47690.1 68416.m05194 microtubule-associated EB1 family protein
           similar to SP|Q9UPY8 Microtubule-associated protein
           RP/EB family member 3 (Protein EB3) {Homo sapiens};
           contains Pfam profile PF03271: EB1 protein
          Length = 276

 Score = 90.6 bits (215), Expect = 9e-19
 Identities = 43/89 (48%), Positives = 58/89 (65%), Gaps = 3/89 (3%)
 Frame = +3

Query: 261 CQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDKIVPIDKLVKGRFQDN 440
           CQ +DM FPG VPM ++ F    E++ IQN+K+LQ  F K+ + K + I++LVKGR  DN
Sbjct: 44  CQMLDMTFPGVVPMHKVNFDAKNEYDMIQNYKVLQDVFNKLKITKPLEINRLVKGRPLDN 103

Query: 441 FEFLQWFKKFFDANYGGAA---YDAVRRR 518
            EFLQW K+F D+  GG     Y+ V RR
Sbjct: 104 LEFLQWLKRFCDSINGGIMNENYNPVERR 132



 Score = 32.7 bits (71), Expect = 0.25
 Identities = 11/28 (39%), Positives = 20/28 (71%)
 Frame = +1

Query: 172 LSRHDMLAWVNDCLQSNFAKIEELCTGA 255
           + R+++L W+ND L  N +++EE  +GA
Sbjct: 14  VGRNEILTWINDRLHLNLSRVEEAASGA 41


>At2g03200.1 68415.m00273 aspartyl protease family protein contains
           Pfam domain, PF00026: eukaryotic aspartyl protease
          Length = 461

 Score = 30.3 bits (65), Expect = 1.3
 Identities = 14/30 (46%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
 Frame = -3

Query: 621 NGLGSSCDFGDRGDGFLSGSGPRG-GRAPV 535
           +G+G  C   + GDGF  GSG  G GR P+
Sbjct: 212 SGIGFGCGVENEGDGFSQGSGLVGLGRGPL 241


>At5g45400.1 68418.m05579 replication protein, putative similar to
           replication protein A 70kDa [Oryza sativa (japonica
           cultivar-group)] GI:13536993; contains InterPro entry
           IPR004365: OB-fold nucleic acid binding domain, PF04057:
           Replication factor-A protein 1, N-terminal domain
          Length = 853

 Score = 28.3 bits (60), Expect = 5.4
 Identities = 14/47 (29%), Positives = 20/47 (42%), Gaps = 1/47 (2%)
 Frame = +1

Query: 157 VTSENLSRHDMLAWVNDCLQSNFAKIEELC-TGAPIASSWTCCSLAV 294
           +  E L   +   W+  C   +F K+E  C T  PI +    CS  V
Sbjct: 569 IKDEKLGTSEKPDWITVCATISFMKVENFCYTACPIMNGDRPCSKKV 615


>At3g19090.1 68416.m02426 RNA-binding protein, putative similar to
           RNA-binding protein homolog GB:AAF00075 GI:6449448 from
           [Brassica napus]; contains InterPro entry IPR000504:
           RNA-binding region RNP-1 (RNA recognition motif) (RRM)
          Length = 455

 Score = 27.9 bits (59), Expect = 7.1
 Identities = 19/69 (27%), Positives = 31/69 (44%), Gaps = 1/69 (1%)
 Frame = -1

Query: 293 TAREQHVHELAIGAPVHNSSIFAKFDWRQSFTHASISCRD-RFSDVTFVEYTFTAIFTMY 117
           TA+ QHVH+ A    + N ++        S +  ++   D R   V  VEY FT +  + 
Sbjct: 104 TAQHQHVHDPAAAFYISNPAVQFPASQNSSSSSKNLLSDDLRLKIVKQVEYQFTDMSLLA 163

Query: 116 QPLIKKFLT 90
              I K ++
Sbjct: 164 NESISKHIS 172


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,124,680
Number of Sequences: 28952
Number of extensions: 354099
Number of successful extensions: 910
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 881
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 910
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1555552968
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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