BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021735 (716 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g67270.1 68418.m08480 microtubule-associated EB1 family prote... 96 2e-20 At5g62500.1 68418.m07844 microtubule-associated EB1 family prote... 91 5e-19 At3g47690.1 68416.m05194 microtubule-associated EB1 family prote... 91 9e-19 At2g03200.1 68415.m00273 aspartyl protease family protein contai... 30 1.3 At5g45400.1 68418.m05579 replication protein, putative similar t... 28 5.4 At3g19090.1 68416.m02426 RNA-binding protein, putative similar t... 28 7.1 >At5g67270.1 68418.m08480 microtubule-associated EB1 family protein similar to SP|Q9UPY8 Microtubule-associated protein RP/EB family member 3 (Protein EB3) {Homo sapiens}; contains Pfam profiles PF00307: Calponin homology (CH) domain, PF03271: EB1 protein Length = 329 Score = 96.3 bits (229), Expect = 2e-20 Identities = 43/95 (45%), Positives = 62/95 (65%), Gaps = 1/95 (1%) Frame = +3 Query: 258 YCQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDKIVPIDKLVKGRFQD 437 +CQ MD + PG+VPM ++ F E+E IQN+K+LQ F K+ + K + + KLVKGR D Sbjct: 43 HCQLMDSVHPGTVPMHKVNFDAKSEYEMIQNYKVLQDVFNKLKITKHIEVSKLVKGRPLD 102 Query: 438 NFEFLQWFKKFFDANYGGA-AYDAVRRRRSAHGAR 539 N EF+QW KK+ D+ GG Y A+ RR ++ G + Sbjct: 103 NLEFMQWMKKYCDSVNGGQHNYHALERREASKGGK 137 Score = 38.7 bits (86), Expect = 0.004 Identities = 14/28 (50%), Positives = 21/28 (75%) Frame = +1 Query: 172 LSRHDMLAWVNDCLQSNFAKIEELCTGA 255 + R ++LAW+N LQ N +K+EE C+GA Sbjct: 14 VGRSEILAWINSTLQLNLSKVEEACSGA 41 >At5g62500.1 68418.m07844 microtubule-associated EB1 family protein similar to EBF3-S (Microtubule-associated protein) [Homo sapiens] GI:12751131; contains Pfam profiles PF00307: Calponin homology (CH) domain, PF03271: EB1 protein Length = 293 Score = 91.5 bits (217), Expect = 5e-19 Identities = 42/89 (47%), Positives = 59/89 (66%), Gaps = 3/89 (3%) Frame = +3 Query: 261 CQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDKIVPIDKLVKGRFQDN 440 CQ +DM FPG VPM ++ F+ E+E IQN+K++Q F K+ + K + +++LVKGR DN Sbjct: 44 CQMLDMTFPGVVPMHKVNFEAKNEYEMIQNYKVMQEVFTKLKITKPLEVNRLVKGRPLDN 103 Query: 441 FEFLQWFKKFFDANYGGAA---YDAVRRR 518 EFLQW K+F D+ GG Y+ V RR Sbjct: 104 LEFLQWLKRFCDSINGGIMNENYNPVERR 132 Score = 33.5 bits (73), Expect = 0.14 Identities = 12/28 (42%), Positives = 21/28 (75%) Frame = +1 Query: 172 LSRHDMLAWVNDCLQSNFAKIEELCTGA 255 + R+++L+W+ND L N ++IEE +GA Sbjct: 14 VGRNEILSWINDRLHLNLSRIEEAASGA 41 >At3g47690.1 68416.m05194 microtubule-associated EB1 family protein similar to SP|Q9UPY8 Microtubule-associated protein RP/EB family member 3 (Protein EB3) {Homo sapiens}; contains Pfam profile PF03271: EB1 protein Length = 276 Score = 90.6 bits (215), Expect = 9e-19 Identities = 43/89 (48%), Positives = 58/89 (65%), Gaps = 3/89 (3%) Frame = +3 Query: 261 CQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDKIVPIDKLVKGRFQDN 440 CQ +DM FPG VPM ++ F E++ IQN+K+LQ F K+ + K + I++LVKGR DN Sbjct: 44 CQMLDMTFPGVVPMHKVNFDAKNEYDMIQNYKVLQDVFNKLKITKPLEINRLVKGRPLDN 103 Query: 441 FEFLQWFKKFFDANYGGAA---YDAVRRR 518 EFLQW K+F D+ GG Y+ V RR Sbjct: 104 LEFLQWLKRFCDSINGGIMNENYNPVERR 132 Score = 32.7 bits (71), Expect = 0.25 Identities = 11/28 (39%), Positives = 20/28 (71%) Frame = +1 Query: 172 LSRHDMLAWVNDCLQSNFAKIEELCTGA 255 + R+++L W+ND L N +++EE +GA Sbjct: 14 VGRNEILTWINDRLHLNLSRVEEAASGA 41 >At2g03200.1 68415.m00273 aspartyl protease family protein contains Pfam domain, PF00026: eukaryotic aspartyl protease Length = 461 Score = 30.3 bits (65), Expect = 1.3 Identities = 14/30 (46%), Positives = 18/30 (60%), Gaps = 1/30 (3%) Frame = -3 Query: 621 NGLGSSCDFGDRGDGFLSGSGPRG-GRAPV 535 +G+G C + GDGF GSG G GR P+ Sbjct: 212 SGIGFGCGVENEGDGFSQGSGLVGLGRGPL 241 >At5g45400.1 68418.m05579 replication protein, putative similar to replication protein A 70kDa [Oryza sativa (japonica cultivar-group)] GI:13536993; contains InterPro entry IPR004365: OB-fold nucleic acid binding domain, PF04057: Replication factor-A protein 1, N-terminal domain Length = 853 Score = 28.3 bits (60), Expect = 5.4 Identities = 14/47 (29%), Positives = 20/47 (42%), Gaps = 1/47 (2%) Frame = +1 Query: 157 VTSENLSRHDMLAWVNDCLQSNFAKIEELC-TGAPIASSWTCCSLAV 294 + E L + W+ C +F K+E C T PI + CS V Sbjct: 569 IKDEKLGTSEKPDWITVCATISFMKVENFCYTACPIMNGDRPCSKKV 615 >At3g19090.1 68416.m02426 RNA-binding protein, putative similar to RNA-binding protein homolog GB:AAF00075 GI:6449448 from [Brassica napus]; contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) Length = 455 Score = 27.9 bits (59), Expect = 7.1 Identities = 19/69 (27%), Positives = 31/69 (44%), Gaps = 1/69 (1%) Frame = -1 Query: 293 TAREQHVHELAIGAPVHNSSIFAKFDWRQSFTHASISCRD-RFSDVTFVEYTFTAIFTMY 117 TA+ QHVH+ A + N ++ S + ++ D R V VEY FT + + Sbjct: 104 TAQHQHVHDPAAAFYISNPAVQFPASQNSSSSSKNLLSDDLRLKIVKQVEYQFTDMSLLA 163 Query: 116 QPLIKKFLT 90 I K ++ Sbjct: 164 NESISKHIS 172 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,124,680 Number of Sequences: 28952 Number of extensions: 354099 Number of successful extensions: 910 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 881 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 910 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1555552968 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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