Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021734
(716 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g31180.2 68417.m04427 aspartyl-tRNA synthetase, putative / as... 66 2e-11
At4g31180.1 68417.m04426 aspartyl-tRNA synthetase, putative / as... 66 2e-11
At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative / as... 66 2e-11
At4g22430.1 68417.m03241 hypothetical protein 34 0.082
At4g34440.1 68417.m04894 protein kinase family protein contains ... 31 0.58
At4g28990.1 68417.m04143 RNA-binding protein-related contains we... 31 0.58
At2g42840.2 68415.m05305 protodermal factor 1 (PDF1) identical t... 30 1.8
At2g42840.1 68415.m05304 protodermal factor 1 (PDF1) identical t... 30 1.8
At5g05840.1 68418.m00642 expressed protein contains Pfam profile... 28 7.1
At5g23050.1 68418.m02695 acyl-activating enzyme 17 (AAE17) nearl... 27 9.4
At1g67590.1 68414.m07700 remorin family protein contains Pfam do... 27 9.4
At1g55255.1 68414.m06311 zinc finger (C3HC4-type RING finger) fa... 27 9.4
>At4g31180.2 68417.m04427 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative similar to
Aspartyl-tRNA synthetase (Aspartate--tRNA ligase)
(AspRS) [Homo sapiens] GI:20178330
Length = 558
Score = 66.5 bits (155), Expect = 2e-11
Identities = 33/71 (46%), Positives = 49/71 (69%), Gaps = 4/71 (5%)
Frame = +1
Query: 514 KDVELVAHQIWTVSSARSQLPLQIEDAARPEKSDEPE----ALKIRVNQDTRLDNRVLDL 681
+ VE+ +++ ++ + ++LPL +EDAAR E E + RVNQDTRL+ RVLDL
Sbjct: 185 QQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYRVLDL 244
Query: 682 RTPANQAVFRI 714
RTPANQA+F++
Sbjct: 245 RTPANQAIFQL 255
Score = 53.2 bits (122), Expect = 2e-07
Identities = 29/71 (40%), Positives = 42/71 (59%), Gaps = 1/71 (1%)
Frame = +2
Query: 290 VWVRARLQTSR-AKGKQCFAVLRQTSSTVQLLVSVNEQRKISKQMVKFTGNITRESIVDV 466
V +R R+ T+R K F VLR++ STVQ +VS +E+ K+ MVK+ ++RES VDV
Sbjct: 110 VLIRGRVHTNRPTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDV 169
Query: 467 EASVVRTAAPV 499
V P+
Sbjct: 170 IGVVTLPKEPL 180
>At4g31180.1 68417.m04426 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative similar to
Aspartyl-tRNA synthetase (Aspartate--tRNA ligase)
(AspRS) [Homo sapiens] GI:20178330
Length = 558
Score = 66.5 bits (155), Expect = 2e-11
Identities = 33/71 (46%), Positives = 49/71 (69%), Gaps = 4/71 (5%)
Frame = +1
Query: 514 KDVELVAHQIWTVSSARSQLPLQIEDAARPEKSDEPE----ALKIRVNQDTRLDNRVLDL 681
+ VE+ +++ ++ + ++LPL +EDAAR E E + RVNQDTRL+ RVLDL
Sbjct: 185 QQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYRVLDL 244
Query: 682 RTPANQAVFRI 714
RTPANQA+F++
Sbjct: 245 RTPANQAIFQL 255
Score = 53.2 bits (122), Expect = 2e-07
Identities = 29/71 (40%), Positives = 42/71 (59%), Gaps = 1/71 (1%)
Frame = +2
Query: 290 VWVRARLQTSR-AKGKQCFAVLRQTSSTVQLLVSVNEQRKISKQMVKFTGNITRESIVDV 466
V +R R+ T+R K F VLR++ STVQ +VS +E+ K+ MVK+ ++RES VDV
Sbjct: 110 VLIRGRVHTNRPTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDV 169
Query: 467 EASVVRTAAPV 499
V P+
Sbjct: 170 IGVVTLPKEPL 180
>At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative simialr to
aspartate-tRNA ligase (EC 6.1.1.12) from Drosophila
melanogaster GI:4512034, Homo sapiens SP|P14868, Rattus
norvegicus SP|P15178; contains Pfam profile PF00152 tRNA
synthetases class II (D, K and N)
Length = 532
Score = 66.5 bits (155), Expect = 2e-11
Identities = 36/71 (50%), Positives = 48/71 (67%), Gaps = 4/71 (5%)
Frame = +1
Query: 514 KDVELVAHQIWTVSSARSQLPLQIEDAARPE----KSDEPEALKIRVNQDTRLDNRVLDL 681
+ VE+ +++ +S + LPL +EDAAR E KS + RV QDTRL+NRVLD+
Sbjct: 159 QQVEIHVRKMYCLSRSLPNLPLVVEDAARSESDIEKSGKDGKQAARVLQDTRLNNRVLDI 218
Query: 682 RTPANQAVFRI 714
RTPANQA+FRI
Sbjct: 219 RTPANQAIFRI 229
Score = 49.2 bits (112), Expect = 3e-06
Identities = 26/80 (32%), Positives = 45/80 (56%)
Frame = +2
Query: 260 NLSIKMDGQTVWVRARLQTSRAKGKQCFAVLRQTSSTVQLLVSVNEQRKISKQMVKFTGN 439
NL ++ G V +R RL +R G + F +LR++ TVQ +V E+ ++ M+KF
Sbjct: 78 NLVEEIVGSEVSIRGRLHKNRLVGTKLFVILRESGFTVQCVV---EETRVGANMIKFVKQ 134
Query: 440 ITRESIVDVEASVVRTAAPV 499
++RES+V++ V P+
Sbjct: 135 LSRESVVELIGVVSHPKKPL 154
>At4g22430.1 68417.m03241 hypothetical protein
Length = 348
Score = 34.3 bits (75), Expect = 0.082
Identities = 18/68 (26%), Positives = 30/68 (44%), Gaps = 3/68 (4%)
Frame = -3
Query: 282 PSILILKFSNQCKPCHDVPLTIGSFSYSHKVLLRSQALIPLMAELMKPYARLSFV---VS 112
P +++L F + K CH +PL + + + L S + + L +P L V +
Sbjct: 162 PGLMVLDFYGKPKDCHFIPLPDHALNRNKTCLATSSGFVMYIKTLAQPGGNLLKVWRLID 221
Query: 111 QRFWQLFW 88
WQL W
Sbjct: 222 DSAWQLMW 229
>At4g34440.1 68417.m04894 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 670
Score = 31.5 bits (68), Expect = 0.58
Identities = 22/60 (36%), Positives = 28/60 (46%), Gaps = 3/60 (5%)
Frame = -2
Query: 691 PACADPKPDCRAGCPGSRGSSRLPAHHSSPVWPRPRSAEEAGIWRSIPSIS---DEPPVP 521
P + P P+ G P S G+S P++ SSP P P S + I P IS PP P
Sbjct: 5 PVDSSPAPETSNGTPPSNGTS--PSNESSPPTP-PSSPPPSSISAPPPDISASFSPPPAP 61
>At4g28990.1 68417.m04143 RNA-binding protein-related contains weak
similarity to Swiss-Prot:Q01844 RNA-binding protein EWS
(EWS oncogene)(Ewing sarcoma breakpoint region 1
protein) [Homo sapiens]
Length = 347
Score = 31.5 bits (68), Expect = 0.58
Identities = 21/74 (28%), Positives = 30/74 (40%), Gaps = 1/74 (1%)
Frame = +3
Query: 486 RPPQSNRAN*GRGTGGSSDMDGIERQIPA-SSADRGRGQTGEE**AGSLEDPREPGHPAR 662
RP SN GR G + + R P+ + +DR +G G S P G+
Sbjct: 23 RPSGSNDREDGRNAAGDYEPGEVSRDRPSFNRSDRYKGDNGGHRTRASSSSPGRRGYEDH 82
Query: 663 QSGFGSAHAG*PGR 704
+ G H+G P R
Sbjct: 83 KHGSDLNHSGVPPR 96
>At2g42840.2 68415.m05305 protodermal factor 1 (PDF1) identical to
protodermal factor 1 [Arabidopsis thaliana]
gi|4929130|gb|AAD33869
Length = 306
Score = 29.9 bits (64), Expect = 1.8
Identities = 15/41 (36%), Positives = 19/41 (46%)
Frame = -2
Query: 670 PDCRAGCPGSRGSSRLPAHHSSPVWPRPRSAEEAGIWRSIP 548
P C G P S S+ P+H S+P P P G + S P
Sbjct: 103 PPCNCGSPPSHPST--PSHPSTPSHPTPSHPPSGGYYSSPP 141
>At2g42840.1 68415.m05304 protodermal factor 1 (PDF1) identical to
protodermal factor 1 [Arabidopsis thaliana]
gi|4929130|gb|AAD33869
Length = 306
Score = 29.9 bits (64), Expect = 1.8
Identities = 15/41 (36%), Positives = 19/41 (46%)
Frame = -2
Query: 670 PDCRAGCPGSRGSSRLPAHHSSPVWPRPRSAEEAGIWRSIP 548
P C G P S S+ P+H S+P P P G + S P
Sbjct: 103 PPCNCGSPPSHPST--PSHPSTPSHPTPSHPPSGGYYSSPP 141
>At5g05840.1 68418.m00642 expressed protein contains Pfam profile
PF04788: Protein of unknown function (DUF620);
expression supported by MPSS
Length = 439
Score = 27.9 bits (59), Expect = 7.1
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = +1
Query: 517 DVELVAHQIWTVSSARSQLPLQIEDA 594
+VE++ H +W S R+ L +Q+ED+
Sbjct: 275 NVEIIRHTVWGCFSQRTGLLIQLEDS 300
>At5g23050.1 68418.m02695 acyl-activating enzyme 17 (AAE17) nearly
identical to acyl-activating enzyme 17 [Arabidopsis
thaliana] GI:29893266; similar to acetyl-CoA synthetase
[SP|P27095] from Methanothrix soehngenii; contains Pfam
AMP-binding enzyme domain PF00501; identical to cDNA
acyl-activating enzyme 17 (At5g23050) GI:29893265
Length = 721
Score = 27.5 bits (58), Expect = 9.4
Identities = 19/74 (25%), Positives = 33/74 (44%)
Frame = +1
Query: 421 GQIHRQHHERVDSRRGGFGREDGRPSRIVPIKDVELVAHQIWTVSSARSQLPLQIEDAAR 600
GQI R+H + + G+ R GR + + +++ + +I V ++ L+
Sbjct: 580 GQILRRHGDLFERTSKGYYRAHGRADDTMNLGGIKVGSIEIERVCNSVDDSVLETAAIGV 639
Query: 601 PEKSDEPEALKIRV 642
P S PE L I V
Sbjct: 640 PPPSGGPEQLVIAV 653
>At1g67590.1 68414.m07700 remorin family protein contains Pfam
domain, PF03763: Remorin, C-terminal region
Length = 347
Score = 27.5 bits (58), Expect = 9.4
Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
Frame = -2
Query: 670 PDCRAGCPGSRGSSRLPAHHSSPVW-PRPRSAEEAGIWRS 554
P+ G +GS+R P HH S + P P ++A W S
Sbjct: 17 PEISNGFEFQKGSNRTPNHHRSTMGKPAPSKWDDAQKWLS 56
>At1g55255.1 68414.m06311 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 383
Score = 27.5 bits (58), Expect = 9.4
Identities = 16/79 (20%), Positives = 42/79 (53%), Gaps = 1/79 (1%)
Frame = +2
Query: 278 DGQTVWVRARLQTSRAKGKQCFAVLRQTSST-VQLLVSVNEQRKISKQMVKFTGNITRES 454
D + W+++ + +S + +Q + R+T ++L E++K+ +++++ +
Sbjct: 251 DKEFRWLKSAVSSSEKEYEQ---ISRRTDDIKLELDDERREKKKLEEELMELNKELEELG 307
Query: 455 IVDVEASVVRTAAPVESCQ 511
VEA++VR V++C+
Sbjct: 308 SESVEAAIVRLQEEVKNCK 326
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,794,269
Number of Sequences: 28952
Number of extensions: 303477
Number of successful extensions: 954
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 905
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 949
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1555552968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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