Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV021730
(720 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 140 2e-34
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 132 5e-32
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 118 1e-27
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 115 6e-27
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 105 8e-24
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 58 2e-09
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 46 7e-06
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 33 0.031
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.88
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 27 2.7
SPBC365.02c |cox10||protoheme IX farnesyltransferase|Schizosacch... 25 8.2
SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 25 8.2
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 25 8.2
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 140 bits (338), Expect = 2e-34
Identities = 64/87 (73%), Positives = 74/87 (85%)
Frame = +3
Query: 255 LIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAE 434
LIGRKF+D VQ+DMKHWPF+V+S GKP ++V YKGE KTF PEE+SSMVL KM+ETAE
Sbjct: 71 LIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSMVLMKMRETAE 130
Query: 435 AYLGKTVQNAVITVPAYFNDSQRQAQK 515
AYLG V +AV+TVPAYFNDSQRQA K
Sbjct: 131 AYLGGKVTDAVVTVPAYFNDSQRQATK 157
Score = 129 bits (311), Expect = 5e-31
Identities = 59/67 (88%), Positives = 64/67 (95%)
Frame = +1
Query: 55 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 234
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63
Query: 235 TIFDAKR 255
TIFDAKR
Sbjct: 64 TIFDAKR 70
Score = 128 bits (310), Expect = 6e-31
Identities = 60/73 (82%), Positives = 67/73 (91%)
Frame = +2
Query: 500 KTSTKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILTIED 679
+ +TKDAG I+GLNVLRIINEPTAAAIAYGLD+ GE NVLIFDLGGGTFDVS+LTIE+
Sbjct: 153 RQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESNVLIFDLGGGTFDVSLLTIEE 212
Query: 680 GIFEVKSTAGDTH 718
GIFEVK+TAGDTH
Sbjct: 213 GIFEVKATAGDTH 225
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 132 bits (319), Expect = 5e-32
Identities = 60/87 (68%), Positives = 72/87 (82%)
Frame = +3
Query: 255 LIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAE 434
LIGR+F D VQ+DMKHWPF+V+ GKP I+V +KGE KTF PEE+SSMVL KM+E+AE
Sbjct: 71 LIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSMVLLKMRESAE 130
Query: 435 AYLGKTVQNAVITVPAYFNDSQRQAQK 515
A+LG V +AV+TVPAYFNDSQRQA K
Sbjct: 131 AFLGGKVTDAVVTVPAYFNDSQRQATK 157
Score = 129 bits (311), Expect = 5e-31
Identities = 59/67 (88%), Positives = 64/67 (95%)
Frame = +1
Query: 55 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 234
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63
Query: 235 TIFDAKR 255
TIFDAKR
Sbjct: 64 TIFDAKR 70
Score = 125 bits (302), Expect = 6e-30
Identities = 59/73 (80%), Positives = 66/73 (90%)
Frame = +2
Query: 500 KTSTKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILTIED 679
+ +TKDAG I+GLNVLRIINEPTAAAIAYGLD+ E NVLIFDLGGGTFDVS+LTIE+
Sbjct: 153 RQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETNVLIFDLGGGTFDVSLLTIEE 212
Query: 680 GIFEVKSTAGDTH 718
GIFEVK+TAGDTH
Sbjct: 213 GIFEVKATAGDTH 225
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 118 bits (283), Expect = 1e-27
Identities = 55/73 (75%), Positives = 63/73 (86%)
Frame = +2
Query: 500 KTSTKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILTIED 679
+ +TKDAG I+GLNVLRIINEPTAAAIAYGLD K +NVLIFDLGGGTFDVS+L I+
Sbjct: 157 RAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKNVLIFDLGGGTFDVSLLKIQG 216
Query: 680 GIFEVKSTAGDTH 718
G+FEV +TAGDTH
Sbjct: 217 GVFEVLATAGDTH 229
Score = 116 bits (279), Expect = 3e-27
Identities = 52/67 (77%), Positives = 59/67 (88%)
Frame = +1
Query: 55 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 234
A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP N
Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67
Query: 235 TIFDAKR 255
T+FDAKR
Sbjct: 68 TVFDAKR 74
Score = 115 bits (276), Expect = 8e-27
Identities = 54/97 (55%), Positives = 69/97 (71%)
Frame = +3
Query: 225 PQQHNIRCQTLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 404
P+ + LIGR++ED Q D+KHWPF+V+ + G P I+V Y GE K F +E+S+M
Sbjct: 65 PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124
Query: 405 VLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQAQK 515
VLTKMKE +EA L K V+ AVITVPAYF+DSQR A K
Sbjct: 125 VLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATK 161
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 115 bits (277), Expect = 6e-27
Identities = 52/97 (53%), Positives = 73/97 (75%)
Frame = +3
Query: 225 PQQHNIRCQTLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 404
P+ + LIGRKF++ T+ D+K +PF +V+D +P ++V G+ K F PEE+S+M
Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153
Query: 405 VLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQAQK 515
+L+KMK+TAEAYLGK V +AV+TVPAYFND+QRQA K
Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATK 190
Score = 114 bits (274), Expect = 1e-26
Identities = 50/73 (68%), Positives = 68/73 (93%)
Frame = +2
Query: 500 KTSTKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILTIED 679
+ +TKDAGTI+GLNV+RI+NEPTAAAIAYGLDK T E++++++DLGGGTFDVS+L+I++
Sbjct: 186 RQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDT-EKHIVVYDLGGGTFDVSLLSIDN 244
Query: 680 GIFEVKSTAGDTH 718
G+FEV +T+GDTH
Sbjct: 245 GVFEVLATSGDTH 257
Score = 112 bits (270), Expect = 4e-26
Identities = 50/66 (75%), Positives = 57/66 (86%)
Frame = +1
Query: 58 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNT 237
+GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ NP NT
Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97
Query: 238 IFDAKR 255
IFD KR
Sbjct: 98 IFDIKR 103
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 105 bits (251), Expect = 8e-24
Identities = 48/73 (65%), Positives = 61/73 (83%)
Frame = +2
Query: 500 KTSTKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILTIED 679
+ +TK AG I+GLNVLR++NEPTAAA+AYGLDKK + V +FDLGGGTFD+SIL + +
Sbjct: 199 RQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK--NDAIVAVFDLGGGTFDISILELNN 256
Query: 680 GIFEVKSTAGDTH 718
G+FEV+ST GDTH
Sbjct: 257 GVFEVRSTNGDTH 269
Score = 90.2 bits (214), Expect = 3e-19
Identities = 43/97 (44%), Positives = 61/97 (62%)
Frame = +3
Query: 225 PQQHNIRCQTLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 404
P+ + LIGR+F++ VQ D+K P+++V + KT+ P ++
Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGGF 166
Query: 405 VLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQAQK 515
+L+KM+ETA YLGK V+NAV+TVPAYFNDSQRQA K
Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATK 203
Score = 83.0 bits (196), Expect = 4e-17
Identities = 39/71 (54%), Positives = 49/71 (69%), Gaps = 1/71 (1%)
Frame = +1
Query: 46 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 222
K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q +
Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108
Query: 223 NPNNTIFDAKR 255
NP NT F KR
Sbjct: 109 NPENTFFATKR 119
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 57.6 bits (133), Expect = 2e-09
Identities = 25/71 (35%), Positives = 44/71 (61%)
Frame = +1
Query: 43 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM 222
++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A
Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62
Query: 223 NPNNTIFDAKR 255
N NT+ KR
Sbjct: 63 NFRNTVGSLKR 73
Score = 53.6 bits (123), Expect = 3e-08
Identities = 29/77 (37%), Positives = 42/77 (54%), Gaps = 4/77 (5%)
Frame = +2
Query: 494 LSKTSTKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGE----RNVLIFDLGGGTFDVS 661
+ + + +A I+GLN LR++N+ AAA+ YG+ K E R V I D G + VS
Sbjct: 155 IQRRALLEAANIAGLNPLRLMNDNAAAALTYGITKTDLPEPESPRRVAIVDFGHSNYSVS 214
Query: 662 ILTIEDGIFEVKSTAGD 712
I+ G F +KST D
Sbjct: 215 IVEFSRGQFHIKSTVCD 231
Score = 44.8 bits (101), Expect = 1e-05
Identities = 27/86 (31%), Positives = 45/86 (52%), Gaps = 1/86 (1%)
Frame = +3
Query: 255 LIGRKFEDATVQ-ADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETA 431
L GR ++D ++ + ++ G KV Y E+ F ++ + TK+K A
Sbjct: 74 LAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAYFTKIKAIA 133
Query: 432 EAYLGKTVQNAVITVPAYFNDSQRQA 509
EA L +V + VI++PA+F D QR+A
Sbjct: 134 EAELIGSVSDVVISIPAWFTDIQRRA 159
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 45.6 bits (103), Expect = 7e-06
Identities = 18/61 (29%), Positives = 35/61 (57%)
Frame = +1
Query: 58 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNT 237
+GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ N +N+
Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADNS 86
Query: 238 I 240
+
Sbjct: 87 V 87
Score = 41.5 bits (93), Expect = 1e-04
Identities = 19/48 (39%), Positives = 29/48 (60%)
Frame = +3
Query: 366 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQA 509
++K E S L ++ E+AE +LG V V++VP YF D+QR+A
Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKA 181
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 33.5 bits (73), Expect = 0.031
Identities = 21/72 (29%), Positives = 33/72 (45%), Gaps = 1/72 (1%)
Frame = +1
Query: 58 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 234
+ ID GT ++ + + G +EI+ R S VAF ER+ G A N P +
Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPAH 84
Query: 235 TIFDAKRSSDVS 270
+I + K D +
Sbjct: 85 SIRNVKELLDTA 96
Score = 30.7 bits (66), Expect = 0.22
Identities = 13/46 (28%), Positives = 26/46 (56%)
Frame = +3
Query: 366 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQR 503
+++ + EE+ +M + AE + + + V+TVP +FN+ QR
Sbjct: 131 DEENYSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQR 176
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 28.7 bits (61), Expect = 0.88
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +2
Query: 50 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 145
HP S W+P LVS+ W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 27.1 bits (57), Expect = 2.7
Identities = 28/126 (22%), Positives = 53/126 (42%)
Frame = -3
Query: 646 TAAEVKDKYISFSSTLFVKTVSNRSSSGFIDDSENVQA*DGTCIFCACL*ESLKYAGTVI 467
+++ + + S SS L T S S+S I S + F + S + I
Sbjct: 565 SSSSIPSTFSSVSSILSSSTSSPSSTSLSISSSST------SSTFSSASTSSPSSISSSI 618
Query: 466 TAFCTVLPR*ASAVSFIFVSTMELTSSGKKVLSSPLYATLILGLPPSLTTSKGQCFMSAC 287
++ T+L + S + +S+ + S +LSS + + + SL+T S+
Sbjct: 619 SSSSTILSSPTPSTSSLMISSSSIISGSSSILSSSIST---IPISSSLSTYSSSVIPSSS 675
Query: 286 TVASSN 269
T+ SS+
Sbjct: 676 TLVSSS 681
>SPBC365.02c |cox10||protoheme IX
farnesyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 387
Score = 25.4 bits (53), Expect = 8.2
Identities = 11/38 (28%), Positives = 19/38 (50%)
Frame = +1
Query: 436 LISAKLCRMQLSRFPRTSMTLKDKHKRCRYHLRLERSP 549
+ +A L Q F S +KD +K+C Y + ++P
Sbjct: 246 ITAAMLFAWQFPHFNAFSTMVKDDYKKCGYQMMAWKNP 283
>SPAC2F7.03c |pom1||DYRK family protein kinase
Pom1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1087
Score = 25.4 bits (53), Expect = 8.2
Identities = 17/66 (25%), Positives = 31/66 (46%)
Frame = +3
Query: 36 QNGKSTRSRNRSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCRQEPG 215
+NGK T +R +S + W P++E R G D + V R + + +EP
Sbjct: 477 RNGKKTPTRTKSRMQQFINWFKPSKE----RSSNGNSDSASPPPVPRLSITRSQVSREP- 531
Query: 216 GDEPQQ 233
++P++
Sbjct: 532 -EKPEE 536
>SPAC823.11 |||sphingosine-1-phosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 25.4 bits (53), Expect = 8.2
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = -3
Query: 136 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCI 32
G W SP S + H ++++ +F +RVL C+
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCM 310
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,196,419
Number of Sequences: 5004
Number of extensions: 68506
Number of successful extensions: 248
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 213
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 243
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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