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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021727
         (674 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g49600.1 68416.m05421 ubiquitin-specific protease 26 (UBP26) ...    31   0.70 
At1g74690.1 68414.m08650 calmodulin-binding family protein low s...    29   2.1  
At2g35890.1 68415.m04406 calcium-dependent protein kinase, putat...    28   6.6  
At3g48800.1 68416.m05329 sterile alpha motif (SAM) domain-contai...    27   8.7  
At1g11160.1 68414.m01278 WD-40 repeat family protein / katanin p...    27   8.7  

>At3g49600.1 68416.m05421 ubiquitin-specific protease 26 (UBP26)
            similar to GI:11993492; RNA binding protein - Homo
            sapiens, EMBL:AB016089 (N-terminus), several ubiquitin
            carboxyl-terminal hydrolases from aa pos. 712
          Length = 1067

 Score = 31.1 bits (67), Expect = 0.70
 Identities = 14/35 (40%), Positives = 19/35 (54%)
 Frame = +3

Query: 138  LCLVRSKSGETLMEDRSDSDVQIDRRNWV*GRKTN 242
            +C VR K     M + SDS  ++DRR     R+TN
Sbjct: 928  VCFVRGKEAPKAMLEASDSSFEVDRRTSKRSRRTN 962


>At1g74690.1 68414.m08650 calmodulin-binding family protein low
           similarity to SF16 protein [Helianthus annuus]
           GI:560150; contains Pfam profile PF00612: IQ
           calmodulin-binding motif
          Length = 587

 Score = 29.5 bits (63), Expect = 2.1
 Identities = 15/32 (46%), Positives = 20/32 (62%)
 Frame = -2

Query: 448 PLGTVIRSPASSFE*AGVLTHLKFENRLRSFR 353
           P  +V + PAS+FE + V T  +FE   RSFR
Sbjct: 282 PKKSVRKVPASNFESSSVQTSFEFEKPKRSFR 313


>At2g35890.1 68415.m04406 calcium-dependent protein kinase, putative
           / CDPK, putative similar to calcium-dependent protein
           kinase, isoform AK1 (CDPK). [Arabidopsis thaliana]
           SWISS-PROT:Q06850; contains protein kinase domain,
           Pfam:PF00069; contains EF hand domain (calcium-binding
           EF-hand), Pfam:PF00036, INTERPRO:IPR002048
          Length = 520

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 11/36 (30%), Positives = 18/36 (50%)
 Frame = +1

Query: 550 MTAGRWPWKSESAKECATTHLPKQPALKMDGAEAFC 657
           +T+  WP  SESAK+     L + P  ++   +  C
Sbjct: 351 LTSDPWPQVSESAKDLIRKMLERNPIQRLTAQQVLC 386


>At3g48800.1 68416.m05329 sterile alpha motif (SAM)
           domain-containing protein contains Pfam profile PF00536:
           SAM domain (Sterile alpha motif)
          Length = 278

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 14/42 (33%), Positives = 21/42 (50%)
 Frame = +1

Query: 211 VGTGYRGERLIEPSRAGSVRSFPQDSWRRYKILKQSHPVKRM 336
           + +GY     ++  R G+V SF   SWR  K +  S   KR+
Sbjct: 99  LSSGYENIGTLDDEREGNVDSFGVGSWRVKKRVGSSAAAKRV 140


>At1g11160.1 68414.m01278 WD-40 repeat family protein / katanin p80
            subunit, putative similar to contains 6 WD-40 repeats
            (PF00400); katanin p80 subunit (GI:3005601)
            [Strongylocentrotus purpuratus]
          Length = 974

 Score = 27.5 bits (58), Expect = 8.7
 Identities = 17/49 (34%), Positives = 24/49 (48%)
 Frame = -2

Query: 517  AFFGALTLRLVHPTAPVLLTKIGPLGTVIRSPASSFE*AGVLTHLKFEN 371
            +  G+ T R V+ +  +LL  +   GTVIRS  S+    GV  H    N
Sbjct: 879  SLLGSRTERPVNVSLDMLLKLVAVFGTVIRSTVSAPRIVGVDLHANERN 927


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,881,562
Number of Sequences: 28952
Number of extensions: 359225
Number of successful extensions: 808
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 793
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 808
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1432596384
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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