BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV021691 (651 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces... 130 2e-31 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 31 0.19 SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha sub... 27 1.8 SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 27 1.8 SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyce... 27 2.3 SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa... 26 5.4 SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 25 9.5 SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||M... 25 9.5 SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces... 25 9.5 >SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1009 Score = 130 bits (313), Expect = 2e-31 Identities = 55/84 (65%), Positives = 68/84 (80%) Frame = +2 Query: 257 LPAYTTHGTIHIVANNQIGFTTDPRHSRSSAYCTDVARVVNAPIFHVNGDNPEAVMHVCN 436 LP Y+T GT+HIV NNQIGFTTDPR +RS+ YCTD+A+ + APIFHVNGD+ EAV +C Sbjct: 422 LPGYSTGGTVHIVINNQIGFTTDPRFARSTPYCTDIAKSMEAPIFHVNGDDVEAVTFICQ 481 Query: 437 VAAEWRATFHKDVVIDIVSYRRNG 508 +AA+WR F DVV+DIV YRR+G Sbjct: 482 LAADWRKAFKTDVVVDIVCYRRHG 505 Score = 91.5 bits (217), Expect = 1e-19 Identities = 48/84 (57%), Positives = 57/84 (67%), Gaps = 1/84 (1%) Frame = +3 Query: 3 SGDVKYHLGTYIERLNRVTNKNIRLAVCANPSHLEAVDPVVQGKTRAEQFYRGDNEG-KK 179 SGDVKYHLG +R + K + L++ ANPSHLEA DPVV GK RA Q Y D ++ Sbjct: 337 SGDVKYHLGMNYQRPTP-SGKRVSLSLVANPSHLEAEDPVVLGKVRAIQHYTSDEASHEQ 395 Query: 180 VMSILLHGDAAFAGQGVVFETMHL 251 M IL+HGDAAFA QGVV+ET L Sbjct: 396 SMGILIHGDAAFAAQGVVYETFGL 419 Score = 44.8 bits (101), Expect = 1e-05 Identities = 20/43 (46%), Positives = 26/43 (60%) Frame = +1 Query: 508 NNEVDEPMFTQPLMYQKIRKTKPVLEKYADQLIAEGVVTAEEV 636 +NE D+P FTQP MY+ I K P + Y QL+ E V+ EV Sbjct: 506 HNETDQPSFTQPRMYKAIAKHPPTFKIYTQQLLQEKTVSKAEV 548 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 30.7 bits (66), Expect = 0.19 Identities = 21/56 (37%), Positives = 27/56 (48%), Gaps = 1/56 (1%) Frame = -3 Query: 586 SPILVWSYESSGT*GAE*TSVRRL-RCSVSPVADDVNDNVLVERGSPFGSHVTNVH 422 SP L W S VRR + S SPV+D V++N+L S S TN+H Sbjct: 147 SPSLTWHTSSGDDSNQNPFFVRRQSQSSTSPVSDSVDENLLSAVSSVTESVETNLH 202 >SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha subunit Pda1|Schizosaccharomyces pombe|chr 1|||Manual Length = 409 Score = 27.5 bits (58), Expect = 1.8 Identities = 21/74 (28%), Positives = 33/74 (44%) Frame = +2 Query: 299 NNQIGFTTDPRHSRSSAYCTDVARVVNAPIFHVNGDNPEAVMHVCNVAAEWRATFHKDVV 478 NN+ G T RSSA R P VNG + AV+ A ++ + ++ Sbjct: 241 NNKYGMGTSAE--RSSAMTEFYKRGQYIPGLLVNGMDVLAVLQASKFAKKYTVENSQPLL 298 Query: 479 IDIVSYRRNGTTKS 520 ++ V+YR G + S Sbjct: 299 MEFVTYRYGGHSMS 312 >SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|Schizosaccharomyces pombe|chr 2|||Manual Length = 1173 Score = 27.5 bits (58), Expect = 1.8 Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 4/54 (7%) Frame = -3 Query: 526 VRRLRCSV----SPVADDVNDNVLVERGSPFGSHVTNVHHGLGIVAVHVEYRSV 377 VRR+ CS + A + + V + G P HVT+ +GLG + +V Y + Sbjct: 1028 VRRVLCSAYFHQAACAKGIGEYVHLRSGMPCHLHVTSSLYGLGYLPDYVIYHEL 1081 >SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 2.3 Identities = 14/37 (37%), Positives = 21/37 (56%) Frame = +2 Query: 488 VSYRRNGTTKSTNRCSLSPSCTRRFVRPNQYWRNTRT 598 V + R+ T + CS PSC +RF R ++ R+ RT Sbjct: 20 VRHIRSHTGEKPFECSY-PSCKKRFTRRDELIRHVRT 55 >SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosaccharomyces pombe|chr 3|||Manual Length = 1213 Score = 25.8 bits (54), Expect = 5.4 Identities = 13/33 (39%), Positives = 18/33 (54%) Frame = -2 Query: 215 ERRVAVQQDRHHLLALVVSPVELLGSGLALHHG 117 E+ VA + LL + E+L GLA+HHG Sbjct: 589 EKAVARLKKEDRLLPQIGRMREMLSRGLAVHHG 621 >SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2670 Score = 25.0 bits (52), Expect = 9.5 Identities = 12/39 (30%), Positives = 16/39 (41%) Frame = +1 Query: 475 CH*HRQLQEKRNNEVDEPMFTQPLMYQKIRKTKPVLEKY 591 C+ Q RN + +M Q + KP LEKY Sbjct: 218 CYYFHQSPNPRNEISQQAELFSKIMAQNVLMAKPALEKY 256 >SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1317 Score = 25.0 bits (52), Expect = 9.5 Identities = 16/56 (28%), Positives = 24/56 (42%), Gaps = 1/56 (1%) Frame = -3 Query: 532 TSVRRLRCSVSPVADDVNDNVLVERGSPFGSHVTNVHHGLGIVAVHV-EYRSVYNA 368 T R +R D++ + L G G H + LGI V V +Y S+ +A Sbjct: 474 TMARPIRALTEAKGHDISIHRLTSFGGAGGQHCAAIAKSLGITQVLVHKYSSILSA 529 >SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces pombe|chr 2|||Manual Length = 466 Score = 25.0 bits (52), Expect = 9.5 Identities = 9/22 (40%), Positives = 15/22 (68%) Frame = +3 Query: 3 SGDVKYHLGTYIERLNRVTNKN 68 +GD+ +H G IE ++R N+N Sbjct: 423 AGDLSFHAGDRIEVVSRTDNQN 444 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,582,600 Number of Sequences: 5004 Number of extensions: 50696 Number of successful extensions: 147 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 145 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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