SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV021691
         (651 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces...   130   2e-31
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb...    31   0.19 
SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha sub...    27   1.8  
SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|...    27   1.8  
SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyce...    27   2.3  
SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa...    26   5.4  
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac...    25   9.5  
SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||M...    25   9.5  
SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces...    25   9.5  

>SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1009

 Score =  130 bits (313), Expect = 2e-31
 Identities = 55/84 (65%), Positives = 68/84 (80%)
 Frame = +2

Query: 257 LPAYTTHGTIHIVANNQIGFTTDPRHSRSSAYCTDVARVVNAPIFHVNGDNPEAVMHVCN 436
           LP Y+T GT+HIV NNQIGFTTDPR +RS+ YCTD+A+ + APIFHVNGD+ EAV  +C 
Sbjct: 422 LPGYSTGGTVHIVINNQIGFTTDPRFARSTPYCTDIAKSMEAPIFHVNGDDVEAVTFICQ 481

Query: 437 VAAEWRATFHKDVVIDIVSYRRNG 508
           +AA+WR  F  DVV+DIV YRR+G
Sbjct: 482 LAADWRKAFKTDVVVDIVCYRRHG 505



 Score = 91.5 bits (217), Expect = 1e-19
 Identities = 48/84 (57%), Positives = 57/84 (67%), Gaps = 1/84 (1%)
 Frame = +3

Query: 3   SGDVKYHLGTYIERLNRVTNKNIRLAVCANPSHLEAVDPVVQGKTRAEQFYRGDNEG-KK 179
           SGDVKYHLG   +R    + K + L++ ANPSHLEA DPVV GK RA Q Y  D    ++
Sbjct: 337 SGDVKYHLGMNYQRPTP-SGKRVSLSLVANPSHLEAEDPVVLGKVRAIQHYTSDEASHEQ 395

Query: 180 VMSILLHGDAAFAGQGVVFETMHL 251
            M IL+HGDAAFA QGVV+ET  L
Sbjct: 396 SMGILIHGDAAFAAQGVVYETFGL 419



 Score = 44.8 bits (101), Expect = 1e-05
 Identities = 20/43 (46%), Positives = 26/43 (60%)
 Frame = +1

Query: 508 NNEVDEPMFTQPLMYQKIRKTKPVLEKYADQLIAEGVVTAEEV 636
           +NE D+P FTQP MY+ I K  P  + Y  QL+ E  V+  EV
Sbjct: 506 HNETDQPSFTQPRMYKAIAKHPPTFKIYTQQLLQEKTVSKAEV 548


>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1334

 Score = 30.7 bits (66), Expect = 0.19
 Identities = 21/56 (37%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
 Frame = -3

Query: 586 SPILVWSYESSGT*GAE*TSVRRL-RCSVSPVADDVNDNVLVERGSPFGSHVTNVH 422
           SP L W   S          VRR  + S SPV+D V++N+L    S   S  TN+H
Sbjct: 147 SPSLTWHTSSGDDSNQNPFFVRRQSQSSTSPVSDSVDENLLSAVSSVTESVETNLH 202


>SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha
           subunit Pda1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 409

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 21/74 (28%), Positives = 33/74 (44%)
 Frame = +2

Query: 299 NNQIGFTTDPRHSRSSAYCTDVARVVNAPIFHVNGDNPEAVMHVCNVAAEWRATFHKDVV 478
           NN+ G  T     RSSA      R    P   VNG +  AV+     A ++     + ++
Sbjct: 241 NNKYGMGTSAE--RSSAMTEFYKRGQYIPGLLVNGMDVLAVLQASKFAKKYTVENSQPLL 298

Query: 479 IDIVSYRRNGTTKS 520
           ++ V+YR  G + S
Sbjct: 299 MEFVTYRYGGHSMS 312


>SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase
            Prp16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1173

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 4/54 (7%)
 Frame = -3

Query: 526  VRRLRCSV----SPVADDVNDNVLVERGSPFGSHVTNVHHGLGIVAVHVEYRSV 377
            VRR+ CS     +  A  + + V +  G P   HVT+  +GLG +  +V Y  +
Sbjct: 1028 VRRVLCSAYFHQAACAKGIGEYVHLRSGMPCHLHVTSSLYGLGYLPDYVIYHEL 1081


>SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 14/37 (37%), Positives = 21/37 (56%)
 Frame = +2

Query: 488 VSYRRNGTTKSTNRCSLSPSCTRRFVRPNQYWRNTRT 598
           V + R+ T +    CS  PSC +RF R ++  R+ RT
Sbjct: 20  VRHIRSHTGEKPFECSY-PSCKKRFTRRDELIRHVRT 55


>SPCC550.03c |||RNA helicase involved in mRNA
           catabolism|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1213

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 13/33 (39%), Positives = 18/33 (54%)
 Frame = -2

Query: 215 ERRVAVQQDRHHLLALVVSPVELLGSGLALHHG 117
           E+ VA  +    LL  +    E+L  GLA+HHG
Sbjct: 589 EKAVARLKKEDRLLPQIGRMREMLSRGLAVHHG 621


>SPAC18G6.05c |||translation elongation regulator Gcn1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2670

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 12/39 (30%), Positives = 16/39 (41%)
 Frame = +1

Query: 475 CH*HRQLQEKRNNEVDEPMFTQPLMYQKIRKTKPVLEKY 591
           C+   Q    RN    +      +M Q +   KP LEKY
Sbjct: 218 CYYFHQSPNPRNEISQQAELFSKIMAQNVLMAKPALEKY 256


>SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1317

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 16/56 (28%), Positives = 24/56 (42%), Gaps = 1/56 (1%)
 Frame = -3

Query: 532 TSVRRLRCSVSPVADDVNDNVLVERGSPFGSHVTNVHHGLGIVAVHV-EYRSVYNA 368
           T  R +R        D++ + L   G   G H   +   LGI  V V +Y S+ +A
Sbjct: 474 TMARPIRALTEAKGHDISIHRLTSFGGAGGQHCAAIAKSLGITQVLVHKYSSILSA 529


>SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 466

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 9/22 (40%), Positives = 15/22 (68%)
 Frame = +3

Query: 3   SGDVKYHLGTYIERLNRVTNKN 68
           +GD+ +H G  IE ++R  N+N
Sbjct: 423 AGDLSFHAGDRIEVVSRTDNQN 444


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,582,600
Number of Sequences: 5004
Number of extensions: 50696
Number of successful extensions: 147
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -